Distribution and Variation Characteristics of Branched Glycerol Dialkyl Glycerol Tetraethers (BrGDGTs) in Sediment Cores Along the Nearshore-to-Offshore Gradient of the East China Sea and Their Correlation with Microbial Community Diversity.

Branched glycerol dialkyl glycerol tetraethers (brGDGTs) are promising molecular biomarkers widely applied in paleoenvironmental reconstructions, including temperature and pH. However, knowledge of the microorganisms responsible for brGDGT production in marine environments remains limited, which constrains the further development and application of brGDGT-based proxies for reconstructing past marine conditions. In this study, both 'living' intact polar lipid-derived brGDGTs (IPL-brGDGTs) and 'fossil' core brGDGTs (CL-brGDGTs), together with bacterial community compositions, were analysed in multiple sediment cores collected along a nearshore-to-offshore transect in the East China Sea (ECS).

Mutation profiling, evolution analysis, molecular dynamics simulation, and functional characterization of Omicron sub-strains.

The ongoing mutation and evolution of SARS-CoV-2 have posed a severe threat to global health, and their functional impact remains to be further characterized. Here, we analyzed the selection pressure from 49 Omicron sub-strains at the gene and amino acid levels. We also examined the impact of mutations on the binding affinity between the receptor binding domain (RBD) and angiotensin-Converting Enzyme 2 (ACE2) and evaluated the immune escape ability of RBD responding to the monoclonal antibodies (mAbs) through molecular dynamics simulation on eight representative Omicron sub-variants (B.

Bench-Stable Carbamoylzinc Pivalates for Modular Access to Amides, Ureas, and Thiocarbamates.

Amide units and relative carbonyl families such as ureas and their derivatives are central important backbones of numerous compounds with activities of relevance to biology or medicinal chemistry. Driven by their prevalence, a general technology that enables sustainable amide-unit synthesis should afford new opportunities for chemical innovation. Generally, stoichiometric quantities of activating reagents, (tri)phosgene and its derivatives, or CO are commonly used in the literature to construct such scaffolds, which represent the drawbacks of these approaches.

Immune checkpoint inhibitors or targeted therapy by mismatch repair status in endometrial cancer: a meta-analysis.

Objective: This study evaluated the benefits of immune checkpoint inhibitors (ICIs) and/or targeted therapies in mismatch repair-deficient (dMMR) and mismatch repair-proficient (pMMR) patients with advanced or recurrent endometrial cancer (EC) via network meta-analysis.

Methods: English databases were searched from inception through January 2025. Randomized controlled trials (RCTs) assessing the efficacy and safety of related therapies for patients with EC stratified by MMR status were included.

Adipocyte RNA-binding protein CELF1 promotes beiging of white fat through stabilizing Dio2 mRNA.

RNA-binding proteins (RBPs) regulate diverse post-transcriptional processes and play roles in adipocyte development; however, their role in white fat beiging remains unclear. Here we identify CUG-BP Elav-like family member 1 (CELF1) as a key RBP promoting beiging of inguinal white adipose tissue in response to cold. Adipocyte-specific Celf1 deficiency impairs cold-induced thermogenic gene expression and reduces energy expenditure.

and activity of quisinostat against .

an opportunistic pathogen, poses severe threats to immunocompromised individuals and fetuses of newly infected pregnant women. The current gold-standard treatment, a combination of pyrimethamine and sulfadiazine, is limited by severe adverse events, necessitating the development of novel therapeutic agents. , a CCK8 assay demonstrated that quisinostat inhibited HeLa cell proliferation in a dose-dependent manner, with a CC of 8.

Integrating Ion Mobility Spectrometry and Mass Spectrometry Imaging for Characterizing the Distribution of Biologically Active Disaccharide Isomers.

The ability to differentiate disaccharide isomers within plant tissues, in a spatially resolved fashion, is required to render a more precise understanding of their biological function within these systems. In this study, we report drift tube ion mobility spectrometry (DTIMS) and trapped ion mobility spectrometry (TIMS) profiling of two important plant disaccharides, sucrose and trehalose, along with their 6-phosphate derivatives. We then map these disaccharides in poplar root and soybean root nodules using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) with premass analysis TIMS.

Ferroptosis is important for replication and virulence and .

The protozoan parasite employs intricate mechanisms to exploit host cells while sustaining their viability, yet its interaction with ferroptosis - an iron-dependent cell death driven by lipid peroxidation - remains poorly defined. Here, we show infection induces ferroptotic hallmarks in RAW264.7 macrophages, including elevated lactate dehydrogenase release, labile Fe accumulation, reactive oxygen species (ROS) generation, and lipid peroxidation.

Prenatal Phenotype in a Neonate with Prader-Willi Syndrome and Literature Review.

Prader-Willi syndrome (PWS) is a rare genetic disease caused by imprinted gene dysfunction, typically involving deletion of the chromosome 15q11.2-q13 region, balanced translocation, or related gene mutations in this region. PWS presents with complex and varied clinical manifestations.

Resection of urethral caruncles with holmium laser vs. conventional excision: a comparative study of minimally invasive outcomes.

Purpose: The purpose of this study was to compare the efficacy and safety of holmium laser resection versus conventional surgical excision for the treatment of urethral caruncle. The study aimed to evaluate the potential advantages of holmium laser, such as its precision and minimal invasiveness, over traditional surgical methods.

Methods: A retrospective cohort study included 116 patients (50 laser vs.

The construction of HMME-PDT efficacy prediction model for port-wine stain based on machine learning algorithms.

Hematoporphyrin monomethyl ether-photodynamic therapy (HMME-PDT) is a safe and effective treatment for port-wine stain (PWS). Comprehensive methods for predicting HMME-PDT efficacy based on clinical factors are lacking. This study aims to develop and validate two machine learning models to predict the therapeutic effect of HMME-PDT for PWS.

The histidine kinase PhpA regulates the biocontrol activity of Pseudomonas bijieensis 2P24 through the Gac/Rsm signaling pathway.

Background: Histidine kinases in rhizosphere bacteria regulate physiological behaviors through two-component systems (TCSs) in response to environmental cues. The RetS-GacS/GacA multikinase network is critical for 2,4-diacetylphloroglucinol (2,4-DAPG) production and biocontrol activity in Pseudomonas bijieensis 2P24. However, the involvement of additional histidine kinases in this regulatory pathway remains unclear.

ANXA1 binds and stabilizes EGFR to promote nasopharyngeal carcinoma radioresistance.

Radioresistance is a serious obstacle to successful nasopharyngeal carcinoma (NPC) treatment. ANXA1 is associated with EGFR and is involved in EGFR-promoting tumors, but mechanism of ANXA1-stabilizing EGFR and its effect on NPC radioresistance are unclear. Here, we report that ANXA1 competes with E3 ubiquitin ligase Cbl for binding EGFR, and increases its stability by inhibiting Cbl-mediated EGFR ubiquitination degradation in NPC cells.

Zebrafish Affects Rod Cell Phototransduction Through Regulating Ca Transport and MAPK Signaling Pathway.

Mutations in the pathogenic gene are known to cause Usher syndrome, affecting both auditory and visual functions. Our previous results provided valuable insights into the mechanisms underlying congenital hearing loss associated with mutations. However, the molecular mechanisms and signaling pathways that influence vision remain largely unknown.

Development of a Recombinant Outer Membrane Vesicles (OMVs)-Based Vaccine Against Helicobacter pylori Infection in Mice.

The current vaccine development for Helicobacter pylori (H. pylori) still faces challenges of weak immune responses stimulated by existing antigens and a lack of safe adjuvants. The modification of the lipopolysaccharide (LPS) structure by H.

Flexible and navigable suction ureteral access sheath versus traditional ureteral access sheath for flexible ureteroscopy in renal and proximal ureteral stones: a meta-analysis of efficacy and safety.

Background: Traditional ureteral access sheaths (T-UAS) are limited by rigidity and lack of suction, potentially increasing complications. Flexible and navigable suction ureteral access sheaths (FANS-UAS) offer improved maneuverability and active suction, but comparative evidence on their efficacy and safety is scarce. This meta-analysis evaluates FANS-UAS versus T-UAS in flexible ureteroscopy (fURS).

The SlGRAS9-SlMYC1 regulatory module controls glandular trichome formation and modulates resilience to pest in tomato.

Trichomes of aerial plant organs contribute to adaptive responses to abiotic and biotic stresses. In horticultural plants, increasing glandular trichome density is an effective breeding strategy to enhance resistance to herbivores through promoting the capacity to produce specialized metabolites. The regulatory mechanisms controlling multicellular trichome formation are only partially understood.

Flexible suction ureteral access sheaths vs. sheathless RIRS for renal and proximal ureteral stones: a comparative study.

Purpose: This study aimed to compare the efficacy and safety of flexible and navigable suction ureteral access sheath (FANS)-assisted retrograde intrarenal surgery (RIRS) vs. sheathless techniques in treating renal/proximal ureteral stones, with a focus on immediate and short-term stone clearance rates, stenting duration, and complications.

Methods: A retrospective cohort analysis was conducted on 115 patients (FANS group: 73; control group: 42) with comparable mean stone diameters (FANS: 14.

AHL-mediated quorum sensing drives microbial community succession and metabolic pathway in algal-bacterial biofilm system.

Microalgae, ammonia-oxidizing bacteria (AOB), and anaerobic ammonium-oxidizing bacteria (AnAOB) have been proven to form an integrated algal-bacterial biofilm system with over 93 % of total nitrogen removal. Compared to conventional nitrification-denitrification process, this system operated without additional organic carbon or aeration. In order to understand the interaction mechanisms between bacteria and algae, this study investigated microbial community succession, the changes in metabolic pathways and the potential role of acyl-homoserine lactone (AHL)-mediated quorum sensing (QS) during the formation of the algae/partial nitrification/anammox biofilm system.

Highly selective electrochemical sensing of hydroquinone and catechol using Co nanoparticles anchored on N-doped carbon nanotube hollow sphere.

Background: Hydroquinone (HQ) and catechol (CC), two important isomers with similar structures, are highly toxic, often coexisting, and impeding each other in the simultaneous detection. Electrochemical technique provides a promising alternative toward the quantification of HQ and CC, due to its inherent advantages in terms of highly sensitive reaction, ease of monitoring, low-cost, simplicity and quick response. Development of a sensing material with outstanding electrocatalytic capabilities and its utilization for the fabrication of an electrochemical sensor for highly selective monitoring of HQ and CC is of great significance.

Attention to the interactions between vincristine and triazole antifungals: perspectives from real-world retrospective analysis and pharmacovigilance assessment.

Background: In patients with hematologic malignancies, the combination of triazole antifungals and vincristine can lead to severe neurological toxicity. We attempted to assess the adverse events (AEs) associated with these interactions, particularly ileus.

Research Design And Methods: We retrieved AEs data from FAERS database (2004-2023) focusing on the combination of four triazoles (fluconazole, itraconazole, voriconazole, posaconazole) with vincristine.

BMAL1-depletion remodels ceramide metabolism to regulate ferroptosis and sorafenib chemosensitivity in acute myeloid leukemia.

Acute myeloid leukemia (AML) is a hematologic malignancy with a poor prognosis. We discovered that BMAL1 is a ferroptosis suppressor. Furthermore, it was also found to be overexpressed in AML patients, affecting the cell cycle and promoting tumor cell growth and progression.

The therapeutic promise of probiotic (BF839) in cancer immunotherapy.

Background: Overwhelming evidence suggests that the gut microbiota modulates tumor response to immune checkpoint inhibitors (ICIs). The probiotic (BF839) was extensively used in China to improve gut microbiota dysbiosis-related symptoms. We hypothesized that probiotic BF839 could enhance tumor sensitivity to ICIs.

Improvement of epilepsy secondary to acquired immunodeficiency syndrome with intestinal microbiota preparations: a case report.

Background: Epilepsy secondary to acquired immunodeficiency syndrome (AIDS) can be challenging to manage. The potential interactions between antiretroviral drugs and antiepileptic drugs may result in the failure of both treatments. Therefore, it is crucial to develop more effective strategies to enhance the clinical outcomes of patients.