Evaluating the potential of GSPT2 and CIRBP as biomarkers in endometrial cancer: Multicenter RT-PCR and IHC study.

Endometrial cancer (EC) is a prevalent gynecological malignancy that imposes significant health and economic burden on women worldwide. The aim of this study was to investigate the expression levels of G1 to S phase transition 2 (GSPT2) and cold-inducible RNA-binding protein (CIRBP) in endometrial cancer tissues relative to normal endometrial tissues and to evaluate their potential as biomarkers for diagnosis and prognosis. We conducted a prospective analysis involving RNA extraction, real-time polymerase chain reaction (RT-PCR), and immunohistochemistry (IHC) to assess gene expression and protein localization.

First Molecular Identification of spp. in Sheep, Beef Cattle, and Dairy Cattle in Shanxi Province, North China.

spp. are common zoonotic intestinal protozoa, which can lead to serious intestinal diseases in both humans and animals through fecal-oral transmission, leading to significant economic losses and public health challenges. To reveal the prevalence of in sheep and cattle in Shanxi Province, North China, fecal samples were collected from 311 sheep, 392 dairy cattle, and 393 beef cattle from three representative counties in the northern, central, and southern regions of Shanxi Province.

A novel cross-priming amplification technique combined with lateral flow strips for rapid and visual detection of zoonotic Toxoplasma gondii.

Toxoplasma gondii, an obligate intracellular protozoan, infects almost all warm-blooded animals and humans, with felines serving as its sole definitive hosts. Cats release T. gondii oocysts into the environment through feces, contributing to environmental contamination that can lead to toxoplasmosis in humans upon exposure through ingestion of contaminated food, water, or soil.

A novel single-tube LAMP-CRISPR/Cas12b method for rapid and visual detection of zoonotic Toxoplasma gondii in the environment.

Background: Toxoplasma gondii oocysts, excreted in cat feces, pose a significant health risk to humans through contaminated soil and water. Rapid and accurate detection of T. gondii in environmental samples is essential for public health protection.

The prevalence and molecular epidemiology of vancomycin-resistant Enterococcus (VRE) carriage in patients admitted to intensive care units in Beijing, China.

Background: Vancomycin-resistant Enterococcus (VRE) can be carried in the gut for a long period and its carriage status is associated with subsequent infections. This study aimed to investigate the frequency of intestinal VRE carriage in intensive care patients in Beijing.

Methods: A multicenter, retrospective cross-sectional study was conducted at six hospitals in Beijing, China.

Real-time PCR for the rapid detection of vanA, vanB and vanM genes.

Background/purpose: To evaluate the ability of quadruple Taqman probe real-time PCR to the detection of vanA, vanB and vanM in enterococcal isolates.

Methods: A total of 343 strains, including 253 vancomycin-resistant enterococcus (VRE) strains and 90 non-VRE strains, were tested by both quadruple Taqman probe real-time PCR and gel-based PCR assay.

Results: When differentiating among three genotypes of vanA, vanB and vanM in VRE strains, the sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), diagnostic accuracy and consistency of the quadruple Taqman probe real-time PCR were all 100%.