Transcript isoform diversity defines molecular subtypes and prognosis in acute myeloid leukemia through long-read sequencing.

Acute myeloid leukemia (AML) is a genetically complex and clinically heterogeneous hematopoietic malignancy. This study employs long-read transcriptome analysis using oxford nanopore technologies sequencing on 60 primary AML bone marrow samples. This approach delivers comprehensive isoform-level resolution of splicing abnormalities and overcomes limitations of short-read sequencing.

Microbiome characteristics associated with lymph node metastasis in laryngeal squamous cell carcinoma.

Lymph node (LN) metastasis is a key prognostic factor in laryngeal squamous cell carcinoma (LSCC). Emerging evidence implicates the role of the microbiome in cancer progression. This study aimed to investigate the microbial features associated with lymph node metastasis in LSCC and their potential for patient stratification.

Identification of epidemiological risk factors associated with missed abortion in polycystic ovary syndrome: a retrospective analysis.

Background: Patients with polycystic ovary syndrome (PCOS) face a greater risk of miscarriage during pregnancy. However, the relationship between PCOS and missed abortion (MA) has not been comprehensively studied.

Method: This retrospective study included 194 pregnant women with PCOS, diagnosed using the 2004 Rotterdam criteria.

tiRNA-HAR contributes to ischemic myocardial injury via facilitating HuR-mediated stability of p53.

Cardiomyocyte death due to heart occlusion of coronary artery is the main driver to myocardial infarction (MI) and subsequent heart failure progression. tsRNA, a small RNA fragment from tRNA, has been shown to be implicated in many physiological and pathological processes by exerting different biological functions, but the roles of tsRNA in ischemic cardiac injury remain to be determined. The present study identified a hypoxia responsive-tiRNA (tiRNA-HAR) was markedly upregulated in ischemic mouse myocardium and hypoxic cardiomyocytes, respectively.

Negative Pressure Tube Drainage for the Management of Pharyngocutaneous Fistulas in Patients Following Total Laryngectomy.

: The treatment of a pharyngocutaneous fistula (PCF) is typically a lengthy and arduous process, often causing significant pain for patients. This study aims to introduce a new method of treating PCF, negative pressure tube drainage (NPTD). : A retrospective study was conducted on 43 patients who developed PCF after laryngectomy.

Preconception body mass index is associated with the risk of spontaneous preterm birth among women with gestational diabetes: A retrospective cohort study.

Objective: Prior research efforts have not effectively clarified the relationship between preconception body mass index (BMI) and spontaneous preterm birth among women with gestational diabetes mellitus (GDM), particularly among Asian women. This study explores the relationship between pre-pregnancy BMI and spontaneous preterm birth among women with GDM, taking into account triacylglycerol (TG), glycated hemoglobin A1c (HbA1c), and gestational weight gain (GWG) levels.

Method: Data from 1116 women with GDM who produced singleton live births were retrospectively analyzed.

Single-cell multiomics reveals a gene regulatory circuit driving leukemia cell differentiation.

Cancer differentiation therapy aims to induce the maturation of neoplastic cells, but the mechanisms regulating cell fate decisions in oncogenic contexts remain unclear. In this study, we integrated single-cell chromatin accessibility and single-cell transcriptome analyses to explore the regulatory trajectories of a classical PML/RARα acute promyeloid leukemia (APL) cell line (NB4) post treatment by all-trans-retinoid acid (ATRA). Our findings indicated that ATRA activated specific PML/RARα-target enhancers to trigger a regulatory circuit composed of a positive feedforward gene regulatory circuit involving two transcription factors, SPI1 and CEBPE.

Influence of early rehabilitation nursing on postoperative rehabilitation of patients with hallux valgus based on information-motivation-behavior skills model.

Objective: To probe the influence of early rehabilitation nursing on postoperative rehabilitation of patients with hallux valgus on the basis of information-motivation-behavior skills (IMB) model.

Methods: Convenience sampling was adopted, and 80 patients with hallux valgus admitted to the Sixth People's Hospital Affiliated to Shanghai Jiaotong University from July 2020 to July 2022 were randomly separated into control group (CG) and observation group (OG) with 40 patients in each group according to the time of admission. Patients in the CG received routine nursing and rehabilitation guidance and follow-up.

A phase 2 pilot study of umbilical cord blood infusion as an adjuvant consolidation therapy in elderly patients with acute myeloid leukemia.

Acute myeloid leukemia (AML) is an aging-related malignancy, with patients aged ≥60 years old facing significantly poorer prognosis. Umbilical cord blood (UCB) has emerged as a promising source with effective anti-aging roles. Here, we conducted a prospective, phase 2, single-arm trial of UCB infusion as an adjuvant consolidation therapy in elderly AML patients (ChiCTR-OPC-15006492).

Efficacy of Sound Touch Elastography in Assessing Subcutaneous Lipohypertrophy from Insulin Injections.

Objective: Lipohypertrophy (LH) is a localized complication of type 2 diabetes mellitus in patients on long-term insulin therapy. Sound touch elastography (STE) is a crucial tool in the quantitative real-time assessment of tissue hardness within specific target regions. This study aims to explore the efficacy of STE in evaluating the hardness of subcutaneous LH at insulin injection sites in individuals with type 2 diabetes mellitus and investigate the correlations between the hardness of LH lesions and various clinical indicators.

Is transcranial alternating current stimulation effective for improving working memory? A three-level meta-analysis.

Working memory, an essential component of cognitive function, can be improved through specific methods. This meta-analysis evaluates the effectiveness of transcranial alternating current stimulation (tACS), an emerging technique for enhancing working memory, and explores its efficacy, influencing factors, and underlying mechanisms. A PRISMA systematic search was conducted.

Revealing the distinct impacts of effectiveness recognition and memory retention on the transfer of creative cognitive reappraisal.

Previous research has shown that creative cognitive reappraisal is highly effective in regulating negative emotions. We conducted three experiments to explore its transferability. In Experiment 1, we observed that free recall performance was better for creative reappraisal compared to non-creative reappraisal.

Glycerol Kinase Drives Hepatic de novo Lipogenesis and Triglyceride Synthesis in Nonalcoholic Fatty Liver by Activating SREBP-1c Transcription, Upregulating DGAT1/2 Expression, and Promoting Glycerol Metabolism.

Glycerol kinase (GK) participates in triglyceride (TG) synthesis by catalyzing glycerol metabolism. Whether GK contributes to nonalcoholic fatty liver (NAFL) is unclear. The expression of hepatic Gk is found to be increased in diet-induced and genetic mouse models of NAFL and is positively associated with hepatic SREBP-1c expression and TG levels.

Mettl1 knockdown alleviates cardiac I/R injury in mice by inactivating the Mettl1-CYLD-P53 positive feedback loop.

The N7-methylguanosine (m7G) methyltransferase Mettl1 has been recently implicated in cardiac repair and fibrosis. In this study we investigated the role of Mettl1 in mouse cardiomyocytes injury and the underlying mechanisms. Cardiac ischemia/reperfusion (I/R) I/R model was established in mice by ligation of the left anterior descending coronary artery (LAD) for 45 min followed by reperfusion for 24 h.

The deficit in cognitive reappraisal capacity in individuals with anxiety or depressive disorders: meta-analyses of behavioral and neuroimaging studies.

Background: The deficit in cognitive reappraisal capacity is a key factor in developing and maintaining emotional disorders such as anxiety disorders and depressive disorders. However, the results from both neuroimaging and behavioral studies are mixed. Therefore, we systematically conducted a series of meta-analyses based on behavioral and neuroimaging studies to clarify this issue.

DeepMineLys: Deep mining of phage lysins from human microbiome.

Vast shotgun metagenomics data remain an underutilized resource for novel enzymes. Artificial intelligence (AI) has increasingly been applied to protein mining, but its conventional performance evaluation is interpolative in nature, and these trained models often struggle to extrapolate effectively when challenged with unknown data. In this study, we present a framework (DeepMineLys [deep mining of phage lysins from human microbiome]) based on the convolutional neural network (CNN) to identify phage lysins from three human microbiome datasets.

The m7G Methyltransferase Mettl1 Drives Cardiac Hypertrophy by Regulating SRSF9-Mediated Splicing of NFATc4.

Cardiac hypertrophy is a key factor driving heart failure (HF), yet its pathogenesis remains incompletely elucidated. Mettl1-catalyzed RNA N7-methylguanosine (m7G) modification has been implicated in ischemic cardiac injury and fibrosis. This study aims to elucidate the role of Mettl1 and the mechanism underlying non-ischemic cardiac hypertrophy and HF.

Does working memory training improve emotion regulation and reduce internalizing symptoms? A pair of three-level meta-analyses.

Background: Emotional dysfunction is a core feature of many mental disorders. Working memory training (WM-T) is promising to improve emotion regulation and reduce internalizing symptoms (anxiety and depressive symptoms), but the results are mixed. Therefore, we conducted meta-analyses to clarify these mixed results.

Multicomponent qualitative and quantitative analysis of Farfarae Flos in serum using UHPLC-Q TOF-MS.

Farfarae Flos is a traditional herb widely employed for treating coughs, bronchitis, and asthmatic disorders. In the current study, we utilized SWATH and IDA data acquisition modes in combination with multiple data processing techniques to identify Farfarae Flos metabolites in mice serum. A total of 56 compounds were characterized, including 31 phenolic acids, 13 flavonoids, 11 sesquiterpenoids and 1 alkaloid.

Familial genetic and environmental transmission of depression: A multi-informant twin family study.

The phenomenon of familial clustering in depression is well established, yet the mechanisms by which depression is transmitted within families remain poorly understood. In the current study, we investigate the familial genetic and environmental transmission of depression by incorporating data from both adolescent twins and their parents. A total of 987 twin families were recruited from the Beijing Twin Study.

The positive feedback loop of the NAT10/Mybbp1a/p53 axis promotes cardiomyocyte ferroptosis to exacerbate cardiac I/R injury.

Ferroptosis is a nonapoptotic form of regulated cell death that has been reported to play a central role in cardiac ischemia‒reperfusion (I/R) injury. N-acetyltransferase 10 (NAT10) contributes to cardiomyocyte apoptosis by functioning as an RNA ac4c acetyltransferase, but its role in cardiomyocyte ferroptosis during I/R injury has not been determined. This study aimed to elucidate the role of NAT10 in cardiac ferroptosis as well as the underlying mechanism.

CRISPR RNA-Guided Transposases Facilitate Dispensable Gene Study in Phage.

Phages provide a potential therapy for multi-drug-resistant (MDR) bacteria. However, a significant portion of viral genes often remains unknown, posing potential dangers. The identification of non-essential genes helps dissect and simplify phage genomes, but current methods have various limitations.

Prevalence and risk factors of oral human papillomavirus infection among 4212 healthy adults in Hebei, China.

Background: Human papillomavirus (HPV) infection is an essential cause of oropharyngeal squamous cell carcinoma that is increasing in incidence worldwide. However, little is known about the epidemiology of oral HPV infection among healthy adults in China.

Methods: A study in northern China was conducted in 2021 as baseline data of Diverse Life-Course Cohort (DLCC).