Preclinical evaluation of antitumor activity and toxicity of TROP2-specific CAR-T cells for treatment of triple-negative breast cancer.

Background: Triple-negative breast cancer (TNBC) represents a subtype of breast cancer with poorest prognosis due to limited effective targeted therapies. Chimeric antigen receptor T cell (CAR-T) therapy has shown remarkable efficacy in treating hematological cancers, but its application in TNBC requires further development. One major obstacle is the lack of suitable tumor-specific target in TNBC.

Classification of Fetal Congenital Heart Disease by Prenatal Ultrasound and Its Diagnostic Value.

Purpose: To evaluate diagnostic value of prenatal ultrasound in classifying fetal congenital heart disease (CHD) and to verify its diagnostic accuracy by comparing its results with postnatal pathological findings.

Methods: This retrospective study analyzed 372 pregnant women with fetal CHD, detected by ultrasound, who underwent interventional prenatal diagnosis. Prenatal CHD cases were classified at three levels.

Neuronal Endothelin a Receptor Mediates Experimental and Clinical Vascular Pain through an Endothelial-Neural Axis.

Vascular dysfunction causes vascular pain (VP), the most common clinical manifestation of prevalent vascular diseases, while the mechanisms remain elusive. Mouse models are developed by mimicking peripheral vascular diseases and combining multiple strategies to demonstrate that primary sensory neuronal endothelin A receptor (ETAR) mediates experimental and clinical VP through an endothelial-neural axis. Endothelial cells (ECs), but not macrophages or smooth muscle cells (SMCs), release endothelin-1 (ET-1) and directly activate primary sensory neurons by binding to ETAR on sensory neurons, resulting in VP.

Parylation dependent nuclear export of HMGB1 via exportin 1 contributes to morphine tolerance.

Background: Nucleocytoplasmic transport has been implicated in chronic pain, particularly through importin-mediated nuclear import. However, the role of nuclear export in opioid-induced adaptations remains unclear. Exportin 1 (XPO1), a key nuclear export protein, has not been studied in the context of morphine tolerance.

Integrated analysis of gut microbiota, fecal and serum metabolites in type 2 diabetes mellitus with peripheral neuropathy.

Purpose: Diabetic peripheral neuropathy (DPN) is one of the most common complications of type 2 diabetes mellitus (T2DM). In recent years, it has been reported that the progression of DPN is associated with altered gut microbiota and serum metabolites. However, the alterations of the gut microbiota and interaction with metabolites are not well understood in DPN patients.

Efficient Somatic Cell Nuclear Transfer by Overcoming Both Pre- and Post-Implantation Epigenetic Barriers.

Mammalian cloning through somatic cell nuclear transfer (SCNT) involves reprogramming terminally differentiated cells into totipotent embryos. Epigenetic barriers inherited from somatic cells impede reprogramming efficiency and lead to low SCNT embryo development rates. Recent studies have identified two primary types of epigenetic barriers in mouse SCNT embryos, defective pre-implantation development due to abnormal gene expression around zygotic genome activation, associated with aberrant H3K9me3, H3K4me3, and histone acetylation, and defective post-implantation development due to loss of H3K27me3-mediated non-canonical imprinting.

The Rhododendron agastum flavonoid 3-O-glycosyltransferase Ra3GT2 contributes to salt and drought stress tolerance through modulating anthocyanin synthesis.

Flavonoid 3-O-glycosyltransferases (3GTs), in charge of transferring UDP-sugars to 3-OH position of small substrates, control a variety of metabolic processes in plants. Extensive research about 3GTs has been centered in model plants, but little in Rhododendron. Here, three 3GT genes (Ra3GT1-3) were isolated from R.

Identification and validation of serum amino acids as diagnostic biomarkers for diabetic peripheral neuropathy.

Background: Diabetic peripheral neuropathy (DPN) is the most prevalent complication of type 2 diabetes mellitus (T2DM). Due to a lack of specific biomarkers, the early diagnosis of this disorder is limited.

Aim: To identify and validate serum amino acids that could discriminate T2DM patients with DPN from those without DPN.

IFN-I-mediated neutropoiesis bias drives neutrophil priming and inflammatory comorbidities.

Local chronic inflammation is increasingly recognized as a driver of systemic inflammatory comorbidities; however, the underlying mechanisms remain incompletely understood. This study investigates the impact of periodontitis on the reprogramming of bone marrow hematopoiesis, with a focus on neutropoiesis bias and its contribution to the exacerbation of arthritis. Single-cell multiomics sequencing was performed on hematopoietic stem and progenitor cells (HSPCs) isolated from control and ligature-induced periodontitis (LIP) mice to characterize transcriptional and epigenetic alterations.

Perovskite as a Promising Candidate for Treating Typical Contaminants in Medical Wastewater: Synthesis, Progress, and Mechanism.

With the fast development of global medical and health undertakings, medical wastewater has increasingly posed a great threat to human survival and ecosystems. This type of wastewater might contain many components, such as drugs, radioactive substances, heavy metals, etc., making it difficult to meet discharge standards.

Leveraging an mRNA Platform for the Development of Vaccines Against Egg Allergy.

Food allergy (FA) poses a major global health issue due to the increasing prevalence and lack of effective prevention strategies. Allergen-specific immunotherapy (AIT) has emerged as a disease-modifying therapy for FA. However, due to long-term treatment duration and unexpected adverse reactions, only a minority of patients benefit from AIT.

Options for postoperative radiation therapy in patients with de novo metastatic breast cancer.

Background: Although meta-analyses have demonstrated survival benefits associated with primary tumor resection in MBC, guidelines lack consensus on the survival benefit of postoperative radiation therapy (RT).

Methods: In this study, we included 1392 patients with de novo metastatic breast cancer (dnMBC) by integrating data from the SEER database (2010-2019) to systematically assess the efficacy of postoperative RT and develop a machine learning-driven prognostic tool. The primary endpoint was overall survival (OS).

An Insight into the Crystal Growth of 7-Aminodeacetoxycephalosporanic Acid from the Perspective of the Growth Unit.

Crystal growth plays a key role in product attributes and downstream processing, whereas insight into the crystal growth mechanism, especially growth unit structures and behaviors, has been limited. In this work, the growth unit structures and behaviors of 7-aminodeacetoxycephalosporanic acid were explored by experiments and molecular simulations. First, its growth units were determined as the monomer MON I and dimers connected by N-H···O═C (AGG I, 1.

3D bioprinting of prefabricated artificial skin with multicomponent hydrogel for skin and hair follicle regeneration.

The timely management of large-scale wounds and the regeneration of skin appendages constitute major clinical issues. The production of high-precision and customizable artificial skin via 3D bioprinting offers a feasible means to surmount the predicament, within which the selection of bioactive materials and seed cells is critical. This study is aimed at employing skin stem cells and multicomponent hydrogels to prefabricate artificial skin through 3D bioprinting, which enables the regeneration of skin and its appendages.

Superior Multimodal Luminescence in a Stable Single-Host Nanomaterial with Large-Scale Synthesis for High-Level Anti-Counterfeiting and Encryption.

Multimode luminescent materials exhibit tunable photon emissions under different excitation or stimuli channels, endowing them high encoding capacity and confidentiality for anti-counterfeiting and encryption. Achieving multimode luminescence into a stable single material presents a promising but remains a challenge. Here, the downshifting/upconversion emissions, color-tuning persistent luminescence (PersL), temperature-dependent multi-color emissions, and hydrochromism are integrated into Er ions doped CsNaYbCl nanocrystals (NCs) by leveraging shallow defect levels and directed energy migration.

Delayed Administration of IGFBP7 Improved Bone Defect Healing via ZO-1 Dependent Vessel Stabilization.

The vascular response following injury is pivotal for successful bone-defect repair but constitutes a major hurdle in the field of regenerative medicine. Throughout this process, vessel stabilization is crucial to provide an adequate nutrient supply and facilitate efficient waste removal. Therefore, this study investigated whether promoting vascular stabilization improves bone defect repair outcomes.

Swelling problems in immobilized filler: an improvement method and comparative study of the effect of different fillers on biotrickling filters.

Immobilized fillers have been increasingly utilized in biotrickling filters (BTFs) due to their positive impact on shock load resistance and recovery performance. However, due to the inherent characteristics of its immobilized carrier, the immobilized filler is prone to swelling during the long-term operation of the system, resulting in increased pressure drop. Polyurethane (PU) sponge was used as the cross-linked skeleton of immobilized filler and compared with direct emulsified cross-linked immobilized filler for treating ethylbenzene gas.

Substrate-Mediated Regulation of Src Expression Drives Osteoclastogenesis Divergence.

Background/objectives: Glass, bone, and dentin are commonly applied substrates for osteoclast cultures; however, the impact of these substrates on osteoclastogenesis remains underexplored. This study aimed to address a significant gap in understanding how different substrates influence the process of osteoclastogenesis.

Methods: RAW 264.

Genomic insights into prenatal diagnosis of congenital heart defects: value of CNV-seq and WES in clinical practice.

This study aimed to assess the efficiency of CNV-seq and WES in detecting genetic cause of congenital heart disease (CHDs) in prenatal diagnoses and to compare CNV detection rate between isolated and non-isolated CHD cases. We conducted a retrospective study of 118 Chinese fetuses diagnosed with CHD by prenatal ultrasound. Participants underwent CNV-seq and, if necessary, WES to detect chromosomal and single nucleotide variations.

The effect of redox fluctuation on carbon mineralization in riparian soil: An analysis of the hotspot zone of reactive oxygen species production.

Riparian zones are important depositional environments at the catchment scale and provide environmental services such as carbon sequestration. This zone is a highly dynamic interface for oxygen and electron exchange, which confers the basis for reactive oxygen species (ROS) production. However, the differences in soil ROS production and their impact on carbon turnover across various redox locations within the riparian zone remain to be fully elucidated.