Bacterial Acetyltransferase Effector AopP2 Primes Effector-Triggered Immunity in Watermelon by Acetylating a Conserved Transcription Factor.

Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli (Pc), threatens global watermelon production, yet genetic resistance remains scarce. This study investigates the potential of non-adapted interaction triggered by Paracidovorax avenae (Pa), a maize pathogen, to combat BFB in watermelon. We demonstrate that Pa strain ATCC 19860 elicits a hypersensitive response (HR) in watermelon via its type III secretion system (T3SS), inducing effector-triggered immunity (ETI).

Transcription Factor OsNF-YC12 Positively Regulates Resistance in Rice through Activation.

Globally, rice () is one of the most important food crops, but its production is threatened by root-knot nematodes (). Although NF-YC transcription factors play important roles in plant growth and development, their involvement in disease resistance has been poorly characterized. In this study, transcriptome analysis revealed that transcription factor OsNF-YC12 was upregulated upon treatment with nematode PAMP (Ascr#18).

Cytochrome reductase 4 efficiently reduces Neuroglobin and Cytoglobin.

Cytoglobin and Neuroglobin are heme-containing proteins expressed in most vertebrates, including mammals, with still not completely defined physiological roles. Most of the putative functions of cytoglobin/neuroglobin, such as oxygen binding or nitric oxide dioxygenation, rely on the heme iron being in the ferrous ( ) oxidation state. Therefore, it is very possible that reducing systems are active in the cell to maintain both proteins in the ferrous state.

Isolation and preliminary pathogenicity of a recent feline astrovirus strain in China.

Feline astroviruses (FeAstVs) have been increasingly detected in cats in recent years, yet their isolation and pathogenicity remain poorly characterized. In this study, we screened 86 feline diarrheal samples and identified FeAstV in 9.3% (8/86) of the cases, most of which were co-infected with feline parvovirus (FPV).

A Piezoelectric-Actuated Variable Stiffness Miniature Rotary Joint.

With the acceleration of industrialization, deformable mechanisms that can adapt to complex environments have gained widespread applications. Joints serve as carriers for transmitting forces and motions between components, and their stiffness significantly influences the static and dynamic characteristics of deformable mechanisms. A variable stiffness joint is crucial for ensuring the safety and reliability of the system, as well as for enhancing environmental adaptability.

Structure and stability analysis of basaltic glasses for the immobilization of simulated waste.

A power reactor in China was used as the subject, and based on the composition of its spent fuel, simulated waste basaltic glasses were prepared by the solid-state melting method. Among them, Ce/Ce, Nd and La are used to simulate Np/Np, Am and U in high-level nuclear waste (HLW), respectively. The structure, thermal stability and leaching characteristics of basaltic glasses with different contents of simulated waste were investigated.

HrpW Modulates Paracidovorax citrulli Virulence and Plant Immunity via ClRAR1 Interaction in Watermelon.

Bacterial fruit blotch (BFB), caused by Paracidovorax citrulli, severely threatens watermelon production. This study investigates the role of HrpW, an atypical harpin in P. citrulli AAC00-1, in bacterial virulence and host immune modulation.

Ralstonia solanacearum injected protein RipH1 targets transcription factor B-box (BBX)-containing protein 31 in Arabidopsis thaliana to manipulate plant immunity.

The type III effector RipH1, a conserved virulence factor in Ralstonia solanacearum, manipulates plant immunity by targeting the transcription factor B-box (BBX)-containing protein 31, AtBBX31 in Arabidopsis thaliana. Here, we demonstrate that RipH1 suppresses reactive oxygen species (ROS) production and triggers programmed cell death (PCD) in multiple plant species, including Nicotiana benthamiana, tomato, and Arabidopsis thaliana. Notably, RipH1 negatively regulates the pathogenicity of R.

Hepatocellular carcinoma cells downregulate PGAM2 via SIRT2-mediated deacetylation modification to enhance aerobic glycolysis.

Phosphoglycerate mutase 2 (PGAM2) is a crucial glycolytic enzyme. Recently, we have found that both the protein and acetylation levels of PGAM2 are down-regulated in hepatocellular carcinoma (HCC) tissues. However, the functional significance of PGAM2 in HCC progression remains poorly characterized.

Designing Flexible Carbon Nanofiber Membranes by Electrospinning and Cross-Linking for Proton Exchange Membrane Fuel Cells.

Traditional carbon-based materials suffer from fragility, low mechanical strength, and electrical conductivity when they are used as a gas diffusion layer (GDL) in proton exchange membrane fuel cells (PEMFCs), resulting in low power density. In this study, a flexible carbon nanofiber membrane (CFM) was studied for use as a GDL, prepared by polyacrylonitrile (PAN) electrospinning with the incorporation of carboxylated multiwalled carbon nanotubes (MWCNTs), polyethylenimine (PEI) impregnation, glutaraldehyde (GA) cross-linking, and thermal treatment. The concentrations of MWCNTs in the electrospinning solution and PEI in the impregnation solution were investigated.

A simultaneous EEG and eye-tracking dataset for remote sensing object detection.

We introduce the EEGET-RSOD, a simultaneous electroencephalography (EEG) and eye-tracking dataset for remote sensing object detection. This dataset contains EEG and eye-tracking data when 38 remote sensing experts located specific objects in 1,000 remote sensing images within a limited time frame. This task reflects the typical cognitive processes associated with human visual search and object identification in remote sensing imagery.

Factors influencing advance care planning among cancer patients: A qualitative study.

Purpose: The study aims to explore the factors influencing advance care planning among cancer patients, recognizing the importance of understanding these factors to enhance patient outcomes and ensure that patients' preferences are honored.

Methods: Conducted from May to November 2023, this qualitative descriptive study employed a phenomenological research approach. In-depth face-to-face interviews and observations were utilized to gather insights into the factors influencing participation in advance care planning, guided by a semi-structured interview outline based on social ecosystem theory.

High TCTA expression is an adverse prognostic biomarker in acute myeloid leukemia.

BackgroundAcute myeloid leukemia (AML) prognosis varies greatly, underscoring the need for novel biomarkers to improve patient stratification. T-cell leukemia translocation-associated gene (TCTA) has emerged as a potential player in hematological malignancies, yet its role in AML remains unexplored.ObjectiveTo investigate the prognostic significance of TCTA in AML and elucidate its functional mechanisms.

Jumping Characteristics of Broiler Breeder Hens at Different Perching Platform Heights.

Excessive mating is a significant welfare concern for floor-reared broiler breeder hens. Perches have shown potential as a solution to mitigate this issue; however, limited research is available on the design parameters of perches. Understanding the jumping characteristics of broiler breeder hens is crucial for designing safe and functional perches.

The Immune Modulatory Role of Surfactants in Infection.

is a prevalent respiratory microbe that causes acute inflammation in the respiratory system. Surfactant proteins (SP), particularly SP-A and SP-D, are essential for the immunological protection against infection. Variant SP-A2 may lead to immune reactions, which could account for the variability in clinical manifestations among individuals.

Adverse event profile of ocular injury associated with JAK inhibitors in patients with rheumatoid arthritis: a disproportionality analysis.

Background: Janus kinase inhibitors (JAKIs) have been approved for the treatment of rheumatoid arthritis (RA) for several years. A recent real-world study has shown the possibility of a relationship between the occurrence of ocular adverse events (OEs) and the use of JAKIs but failed to rule out whether those OEs are related to RA.

Research Design And Methods: A retrospective, pharmacovigilance study using the FDA Adverse Event Reporting System to evaluate OE reports following treatment with JAK inhibitors in patients with RA from Q1 2004 to Q1 2024.

Structure and quantification of Ce/Ce and stability analysis of basaltic glasses for the immobilization of simulated tetravalent amines.

Basaltic glass was prepared via the solid-state melt method, using Ce to simulate tetravalent actinides. The structure, thermal stability and leaching characteristics of basaltic glass with different contents of CeO were investigated. The XRD/SEM-EDX/Raman results showed that the simulated waste loading of CeO in basaltic glass reached ~ 18 wt%, and CeO crystals precipitated when the CeO content reached 20 wt%.

One-pot ligation of multiple mRNA fragments on dsDNA splint advancing regional modification and translation.

Region-specific RNA modifications are crucial for advancing RNA research and therapeutics, including messenger RNA (mRNA)-based vaccines and immunotherapy. However, the predominant method, synthesizing regionally modified mRNAs with short single-stranded DNA (ssDNA) splints, encounters challenges in ligating long mRNA fragments due to the formation of RNA self-folded complex structures. To address this issue, we developed an efficient strategy using an easily obtained long double-stranded DNA (dsDNA) as a ligation splint after in situ denaturing, while parts of this dsDNA are the templates for transcribing mRNA fragments.

Preparation and Performance Study of Composite Aramid Paper for High-Frequency Working Conditions.

When the power converter connects to the high-frequency transformer breaks through the bottleneck and reaches a frequency of 100 kHz or even higher, the high-frequency transformer's inter-turn insulation faces more serious high-frequency discharge and high-temperature problems. In order to improve the service performance of oil-immersed high-frequency transformer insulation paper, composite K-BNNS particles are prepared by ultrasonic stripping, heat treatment, and thermomagnetic stirring. Then, K-BNNS particles are mixed with PMIA (polymeric m-phenylenediamine solution) slurry to produce composite aramid paper.

Immunomodulatory Effects of Lactiplantibacillus plantarum Strain RW1 During Salmonella Infection in Murine Intestinal Epithelial Cells and Dextran Sulfate Sodium-Induced Murine Colitis.

Inflammatory diseases resulting from bacterial infections or inflammatory bowel disease pose significant threats to the health of both animals and humans. Although probiotics have emerged as a crucial preventive and adjunctive therapy for these conditions, the precise mechanisms through which probiotics regulate inflammatory diseases remain incompletely understood. In our previous study, animal-derived Lactiplantibacillus plantarum strain RW1 (L.

Research on doctor-patient communication teaching for oncology residents: a new teaching model.

Background: At present, there is no specific teaching method for doctor-patient communication for oncology residents. This study combined BOPPPS (bridge-in, learning objective, pretest, participatory learning, posttest, and summary) teaching model and SBAR (situation-background-assessment-recommendation) communication model to try a new teaching and assessment model of doctor-patient communication, aiming to explore and improve the teaching method of doctor-patient communication for oncology residents.

Methods: Ninety residents were randomly divided into two groups: the experimental group (n = 45) was trained with the BOPPPS teaching model combined with the SBAR communication model, the routine teaching method was adopted in the control group (n = 45).

Effects of Incubation Light on Behaviour, Growth Performance, Blood Parameters, and Digestive Enzymes in Post-Hatch Layer Chicks.

Manipulation of light during incubation may have an effect on post-hatch chicks through the role of prenatal stage. The effects of providing different wavelengths of light (white, blue, and green lights, dark as control) during incubation on the growth performance, organ development, immune response, stress related hormones, digestive enzymes and behaviour of post-hatch chicks were investigated for 1-42 days. A total of 60 chicks per light treatment in three batches were used in this study.

Development and validation of a nomogram for predicting cognitive frailty in patients on maintenance haemodialysis.

Aims: This study aimed to construct a nomogram for predicting the risk of cognitive frailty in patients on maintenance haemodialysis.

Design: An explorative cross-sectional design was adopted.

Methods: From April 2022 to July 2022, 496 participants were recruited from five haemodialysis centres in Qingdao, Shandong Province, China.