The effect of complex prebiotics on quality and functional properties of synbiotic fermented milk.

Prebiotic-probiotic synergy is vital to fermented milk's overall performance. This study investigates the effects of complex prebiotics (CPs) on the quality and functional properties of probiotic-fermented milk. Results indicated that CPs improved the physicochemical properties and sensory characteristics of fermented milk.

Qula-derived Limosilactobacillus fermentum TD-3 and Lactococcus lactis MQ1-1 alleviate aging-related intestinal barrier dysfunction via microbiota-short-chain fatty acid-AMPK/MLCK-tight junction axis.

The aging process is associated with detrimental effects on the integrity of the intestinal barrier, consequently elevating susceptibility to various diseases. Qula exhibits outstanding nutritional composition and has a characteristic taste profile, positioning it as a prime candidate for probiotic exploration given its rich reservoir of probiotic potential. In the present study, a probiotics screening strategy was developed based on the production of short-chain fatty acids (SCFA) to reverse aging-related intestinal barrier dysfunction.

Enhancing the availability of construction waste as substrate for constructed wetlands-through mixing substrates and adjusting C/N ratio.

Construction waste offers environmental and economic benefits as a substrate for constructed wetlands (CWs), but its adverse effects, particularly elevated pH reducing removal efficiencies (REs), remain poorly understood. This study investigated mitigating these effects by combining waste concrete with other substrates and adjusting influent carbon to nitrogen (C/N) ratios at 3:1 and 7:1. Vertical subsurface flow CW simulators with four treatments-volcanic rock (Vo), waste concrete (Co), a 1:1 volcanic rock/concrete mixture (Fm), and a 3:1 mixture (Tm)-were established, assessing pollutant RE, microbial community functions, and associated risks/potentials.

Characterization and genomics of phage Henu2_3 against K1 Klebsiella pneumoniae and its efficacy in animal models.

The emergence and spread of multidrug-resistant and super-resistant bacterial strains have positioned phage therapy as a highly promising approach for future infection treatments. Klebsiella pneumoniae, a leading cause of clinical infections and hospital-acquired diseases, is responsible for over 95% of infections within the Klebsiella genus. Phages targeting K.

Nonlinear association between gamma-glutamyl transferase to high-density lipoprotein cholesterol ratio and risk of progression from normoglycemia to prediabetes: a 5-year cohort study.

Objective: Current research on the association between the Gamma-glutamyl transferase to high-density lipoprotein ratio (GHR) and the risk of prediabetes (pre-DM) remains scarce. This study aims to explore the potential link between GHR and the risk of progression from normoglycemia to pre-DM.

Methods: This retrospective cohort study included 8,168 individuals who voluntarily underwent health examinations at Shenzhen Dapeng New District Kuichong People's Hospital between January 2018 and December 2023.

Species-level enumeration of low-concentration lactic acid bacteria in breast milk.

It is crucial to identify the distribution of lactic acid bacteria (LAB) in breast milk which is a source of live probiotics for the infant gut. Quantitative PCR (qPCR) is a powerful technique for selectively counting LAB at the species or strain level. However, the limit of quantification (LoQ) of qPCR is inadequate for human milk samples.

HF01 postbiotics reprogram gut microbial tryptophan metabolism to coordinate enterohepatic barrier-insulin signaling axis.

High-fat diet (HFD) disrupts gut microbiota homeostasis, induces intestinal barrier damage and systemic inflammation, ultimately leading to insulin resistance and hepatic glucose metabolism disorders. This study investigated the therapeutic effects of HF01 ( HF01) -derived postbiotic (cell-free supernatant, CFS; defined as metabolites derived from probiotics) on HFD-induced metabolic dysregulation and its regulatory mechanisms via the gut-liver axis. The findings revealed that CFS suppressed pro-inflammatory genera (, , , and ) while enriching beneficial genera such as , , , , and .

Synthesis of SGLT2 inhibitor -glucoside derivatives for enhancing anti-heart failure activity.

SGLT2 inhibitors were derived from the natural product phlorizin and were used clinically to treat heart failure with preserved ejection fraction (HFpEF). In this study, based on phlorizin as the lead compound, tyramine moiety was introduced while retaining the pharmacophoric glucoside, thereby enhancing SGLT2 protein inhibitory activity and improving gastrointestinal absorption properties. We synthesized 11 new -glucoside derivatives and evaluated their anti-heart failure activities.

Design, synthesis and evaluation of anti-heart failure activity of O-glucoside derivatives.

Heart failure (HF) is a progressive disease characterized by persistent or episodic worsening of symptoms, leading to functional deterioration. Clinically, guidelines recommend the use of SGLT2 inhibitors for the treatment of heart failure. However, the SGLT2 inhibitors exist potential risks including weight loss and euglycemic diabetic ketoacidosis.

UV-Induced Mutants of : Improved Biological Parameters, Resistance to Stressful Factors, and Comparative Transcriptomic Analysis.

, a well-known species of entomopathogenic fungi with great potential as a biological control agent, is vulnerable to UV damage, which restricts its use in the field. To improve the fungal resistance to UV irradiation, UV-induced mutant strains of s. l.

Design, synthesis and evaluation of styrylquinolinium derivatives targeting c-MYC /BCL2 G-quadruplexes against breast cancer.

The G-quadruplex (G4) structures in the promoter regions of proto-oncogenes c-MYC and BCL2, which suppress transcription, have emerged as potential therapeutic targets for breast cancer. This study proposes a dual-targeting G4 ligand design strategy to synergistically block their oncogenic functions through simultaneous stabilization of c-MYC and BCL2 G4 structures.Based on the topological features of c-MYC and BCL2G4, fifteen styrylquinoline derivatives were designed and synthesized.

Synergistic effects of polydopamine-coated reduced graphene oxide on osteogenesis and anti-inflammation in periodontitis.

Owing to its extremely high prevalence and the distressing consequence of tooth loss, periodontitis has attracted substantial research attention. In light of these conditions, graphene-based biomaterials have emerged as a potentially promising approach for periodontal regeneration. This study focuses on the synthesis of polydopamine-coated reduced graphene oxide (RGO@PDA), designed to harness the anti-inflammatory properties of dopamine and the osteogenic potential of graphene oxide for synergistic periodontitis treatment.

Steroids, peniciversiols and aspergilosidols from endophytic fungus Aspergillus sp. TZS-Y4.

Endophytic fungi have emerged as a crucial resource for medicinal drug development. The genus Aspergillus, in particular, has yielded diverse bioactive secondary metabolites. In this study, we isolated nine steroids, five peniciversiols, and two aspergilosidols from the endophytic fungus Aspergillus sp.

Recent progress in tuberculosis diagnosis: insights into blood-based biomarkers and emerging technologies.

Tuberculosis (TB) remains a global health challenge, with timely and accurate diagnosis being critical for effective disease management and control. Recent advancements in the field of TB diagnostics have focused on the identification and utilization of blood-based biomarkers, offering a non-invasive, rapid, and scalable approach to disease detection. This review provides a comprehensive overview of the latest progress in blood-based biomarkers for TB, highlighting their potential to revolutionize diagnostic strategies.

Probiotic Combination of HF07 and HF08 Targeting Gut Microbiota-Secondary Bile Acid Metabolism Ameliorates Inflammation and Intestinal Barrier Dysfunction in Aging Colitis.

This study investigated the intestinal protective effects of a probiotic mixture (PM) composed of HF07 and HF08 on d-gal/DSS-induced aging colitis in mice. The PM alleviated age-related colitis symptoms including weight loss, increased disease activity index scores, colonic shortening, and tissue damage. PM supplementation reshaped the gut microbiota by restoring the relative abundances of , , , and -014, thereby enhancing levels of bile acids (BAs) such as alpha-muricholic acid, isolithocholic acid, and ursodeoxycholic acid.

The virulent bacteriophage Henu8 as an antimicrobial synergist against .

As the overuse of antibiotics has not yet been strictly limited in urban areas, drug-resistant has become a fatal pressure for bacteremia treatment. Considering the outstanding performance of bacteriophages , bacteriophages may serve as an alternative to heal chronic refractory infections. In this study, a 49,890 bp double-stranded circular DNA phage, Henu8, was isolated and was able to lyse the group of strains tested in this study.

Molecular interaction mechanisms of Lactobacillus helveticus KLDS1.8701 on construction of yogurt gel network, transformation of milk protein conformation, and formation of characteristic flavor.

The excellent properties of Lactobacillus helveticus KLDS1.8701 (L. helveticus KLDS1.

Design and synthesis of novel styrylquinolinium derivatives for the treatment of breast Cancer: Targeting the c-Myc G-quadruplex.

Inhibiting c-Myc gene expression by targeting the c-Myc G-quadruplex (G4) represents an effective strategy for breast cancer treatment. In order to find ligands that can specifically target the c-Myc G4, we utilized styrylquinolinium as the core element to anchor G4, and proposed three guiding principles for the design and synthesis of G4 ligands. Finally, compound W11 was identified as the compound skeleton which has the potential to target c-Myc G4.

Klotho senses mechanical stimuli and modulates tension-induced osteogenesis.

Delicate external mechanosensing, efficient intracellular mechanotransduction and effective alveolar bone remodeling lay the foundation of orthodontic tooth movement (OTM). Periodontal ligament stem cells (PDLSCs) are thought to be the primary cells that withstand mechanical stimuli and respond to biomechanical signals during orthodontic treatment. Nevertheless, the cellular and molecular mechanisms of orthodontic force-induced mechanosignaling and osteogenesis in PDLSCs still remain unclear.

Enhancing object pose estimation for RGB images in cluttered scenes.

Estimating the 6D pose of objects is crucial for robots to interact with the environment. 6D Object pose estimation from RGB images in a cluttered scene and heavy occlusions is a critical issue. Most existing methods use two stages to estimate object pose: First, extract the object features, and then use the PnP/RANSAC method to estimate object pose.

How can graduate students' research pressure be transformed into motivation and innovative behavior? The role of graduate students' mentorship homegate (or team) support.

Introduction: This study explores how graduate students' mentorship homegate (or team) support (GSMTS) and challenging-hindering pressures impact their intrinsic motivation for research, identification with research roles, and innovative behaviors.

Methods: Data from 548 graduate students were collected using convenience sampling and analyzed using Amos and SPSS statistical software package via questionnaires distributed to universities in SiChuan province of China.

Result: The findings reveal that (1) research stress can not directly and positively predict innovative behaviors among graduate students, while intrinsic research motivation and research role identification mediate the relationship between research stress and graduate students' innovative behavior; (2) hindering research pressure negatively impacts the intrinsic motivation for research, whereas challenging research pressure has a positive effect; (3) GSMTS directly fosters innovative behaviors among graduate students, with intrinsic motivation and roles' identification for research as sequential mediators; and (4) GSMTS positively moderates the relationship between challenging research pressure and both the intrinsic motivation for research and role identity.

Self-Reconstruction of High Entropy Alloys for Efficient Alkaline Hydrogen Evolution.

Alkaline water (HO) electrolysis is currently a commercialized green hydrogen (H) production technology, yet the unsatisfactory hydrogen evolution reaction (HER) performance severely limits its energy conversion efficiency and cost reduction. Herein, PtRuFeCoNi high entropy alloys (HEAs) is synthesized and subsequently exploited electrochemically induced structural oxidation processes to construct self-reconfigurable HEAs, as an efficient alkaline HER catalyst. The optimized self-reconstructed PtRuFeCoNi HEAs with the HEAs and cobalt rutheniate interface (HEAs-CoRuO) exhibits excellent alkaline HER performance, requiring just 11.

Bioactive milk-derived nutrient MFG-E8 ameliorates skeletal muscle atrophy induced by mitochondria damage in aging rats via activating the MAPK/ERK signaling pathway.

Sarcopenia is the age-related loss of muscle and fiber number and decreased regenerative capacity with increased abundance of reactive oxygen species levels and electron transport chain abnormalities. The aim of this study was to investigate the antisarcopenia effect of MFG-E8 in alleviating skeletal muscle dysfunction induced by D-galactose, and reveal the mechanism promoting myoblast cell proliferation and mediating the cell cycle. This in vivo experiment showed that MFG-E8 can improve the antioxidant status and increase soleus muscle mass (35.