Efficacy and safety of utidelone in pretreated patients with metastatic breast cancer in China: a multicenter, real-world study.

Background: Utidelone (UTD1), a genetically engineered epothilone derivative, has been approved in China for use in combination with capecitabine in treating metastatic breast cancer (MBC) patients previously treated with anthracyclines or taxanes.

Objectives: To evaluate the real-world efficacy and safety of UTD1 in Chinese patients with MBC and to explore potential predictors of therapeutic effectiveness.

Design: A multicenter, retrospective, real-world study.

Improvement of Objectivity and Accuracy in Cervical Disc Degeneration Grade Assessment Using Imaging Parameters: A Cross-Sectional Retrospective Study.

Study DesignA cross-sectional retrospective study.ObjectivesThis study aims to analyze the correlation between cervical disc degeneration grade and cervical imaging parameters and improve grade assessment objectivity and accuracy under the Miyazaki disc degeneration grading system.MethodsCervical disc degeneration was graded using the Miyazaki disc degeneration grading system.

m6A-modified circZNF548 regulates exosomal miR-7108-3p to activate CD3CD8 T cells and suppress NSCLC growth by JMY.

Background: Communication between cancer cells and tumor microenvironment (TME) plays a complicated role in cancer malignancy. Circular RNAs (circRNAs), known for their stability and conservation, contribute to TME remodeling in various cancers. This study aims to investigate the role of N6-methyladenosine (m6A)-modified circZNF548 in the proliferation and migration of non-small cell lung cancer (NSCLC) within the TME.

Exploring the Critical Environmental Optima and Biotechnological Prospects of Fungal Fruiting Bodies.

Fruiting body development is a principal mechanism in fungal morphogenesis, which often involves complex interplays of hormonal regulation, gene expression, and metabolic immobilisation influenced by environmental interactions, ultimately leading to the differentiation of multicellular structures. In fungal communities, including ascomycetes and basidiomycetes, fruiting body development ensures protection and facilitates the dispersal of ascospores. Constrained by environmental factors that vary across morphogenetic stages, a thorough synthesis of the critical ecological optima, which primarily regulate the multi-omics footprint encompassing diverse molecular perspectives characterising fruiting body formations, is key.

Heavy metal burden in the urine and cancer risk.

Aims: The purpose of this study was to evaluate the association between exposure to urinary heavy metals and cancer risk in adults in the United States.

Methods: The statistical data for this study were obtained from the National Health and Nutrition Examination Survey (NHANES) spanning 2009-2018. Participants lacking complete data on urinary heavy metals exposure burden and/or cancer status information were excluded.

Dual Role of MtHAC-1 in Regulating Cellulase and Xylanase Production in Myceliophthora thermophila.

Filamentous fungi produce large quantities of cellulase and xylanase as extracellular enzymes to degrade plant-derived polysaccharides. This process is controlled by a complex network of transcription factors (TFs). Here, we present the bZIP TF Mthac-1 exhibiting dual regulatory effects on the production of cellulase and xylanase in Myceliophthora thermophila.

BEBT-209, a primary CDK4 selective inhibitor, for the treatment of HR+/HER2- advanced breast cancer (BECTOP1): a phase 1, multicentre, open-label study.

Purpose: Several cyclin-dependent kinase 4/6 (CDK4/6) inhibitors have been approved for the treatment of hormone receptor-positive (HR+) and human epidermal growth factor receptor 2-negative (HER2-) advanced breast cancer. Despite the side effects that affect patients' quality of life, most patients still opt for CDK4/6 inhibitors due to their significant benefits. However, to further enhance treatment efficacy and safety, new approaches are still needed.

An exploratory framework for EEG-based monitoring of motivation and performance in athletic-like scenarios.

Motivation is a key psychological factor influencing athletic performance, especially in high-intensity disciplines such as track and field. However, traditional assessment methods-ranging from self-report questionnaires to static physiological models-often fail to capture the temporal, individualized, and context-dependent nature of the motivation-performance relationship. In this study, we propose a hybrid EEG-based framework for modeling motivational states and forecasting athletic performance.

Research on farmers' willingness to grow high-quality rice based on the TAM-TPB model: evidence from China.

Introduction: Professional grain farmers play a key role in China's food production, and growing high-quality rice is essential to secure their income. Existing studies have analyzed the willingness of farmers to adopt new rice varieties from a number of perspectives, such as farm characteristics and external factors, and have focused on the overall category of farmers. There are few studies to explore how professional grain farmers induce the planting intention of high-quality rice from the level of subjective psychological mechanism.

A Bibliometric Study of Interleukin Cytokines in the Treatment of Intervertebral Disc Degeneration.

Background: In recent years, the research on the occurrence and development of interleukin in intervertebral disc degeneration has been more and more extensive. The purpose of this study is to predict the future research direction or prospect by analyzing the relevant literature in this field.

Objective: Interleukin family cytokines are becoming more and more important in the research field of intervertebral disc degeneration, but all previous studies only focus on one aspect and are not comprehensive.

Danshensu Ethyl Ester Alleviates LPS-Induced Acute Lung Injury by Targeting the NLRP3 Inflammasome.

Purpose: To investigate whether Danshensu ethyl ester (DEE) can attenuate acute lung injury (ALI) and explore the detailed mechanism.

Methods: The ALI model was induced in mice using LPS. The effects of DEE on lung wet-to-dry weight ratio (W/D), bronchoalveolar lavage fluid (BALF) protein levels, and neutrophil infiltration (neutrophils) were assessed.

Visualized knowledge map of robot application in surgical field: a bibliometric analysis.

This study aims to conduct a bibliometric analysis of the literature related to robotic surgery to understand the research hotspots and development trends in this field. We focus on the fields of robotics and surgery, having conducted a comprehensive literature review utilizing the Web of Science Core Collection (WOSCC) database, covering the period from 1999 to 2024. We quantified the annual publication count using GraphPad Prism.

Genomic Full-Length Sequence of the HLA-DQB1*06:47 Allele Was Identified by PacBio Sequencing.

The complete genomic sequence of HLA-DQB1*06:47 was determined using a PacBio sequencing approach conducted in China.

Geopolymer Modified with Insoluble Calcite and Various Silica Fumes Originated from Different Manufacturing Processes.

It has been proven that silica fume (SF), which is a by-product from the manufacturing of single-crystal silicon, is beneficial for enhancing the mechanical properties, durability, and workability of geopolymers, as it can be quickly dissolved and form silicate-based cementitious phases in alkaline environments. However, the reinforcement mechanism of SF on geopolymer remains unclear due to the chemical complexity of geopolymer and the variety of SF types. Additionally, the solubility of calcite in an alkali environment is quite limited, and thus the formation of the amorphous calcium-based gels will be thwarted due to the lack of soluble calcium ions.

Generation of Plexin-B1 Conditional Knockout Mouse With CRISPR/Cas9 Technology.

Plexins are axon guidance transmembrane receptors that control cytoskeleton and membrane dynamics in development and adult physiology. As plexins are expressed in multiple cell types in various tissues, floxed alleles that enable conditional deletion are needed to facilitate cell type-specific functional analysis. We report here the generation of a conditional floxed allele of Plexin-B1 (gene symbol Plxnb1) in mouse using CRISPR/Cas9 technology to insert two loxP sites flanking critical exons.

Tumor Invasion Distance Based on MRI Is a Novel Prognostic Indicator for I-IIIB Cervical Cancer Patients Treated with Radiotherapy.

This study aimed to identify the prognostic value of tumor invasion distance (TID) based on MRI findings in cervical-cancer (CC) patients treated with radiotherapy (RT). : A total of 218 CC patients diagnosed at Fujian Cancer Hospital from December 2018 to December 2019 were included in the study. Cox regression analyses were conducted to identify independent prognostic factors for overall survival (OS), including low 1/3 vaginal involvement, a longer TID, and RT without chemotherapy.

Development of Multiplex qPCR Method for Accurate Detection of Enzyme-Producing Psychrotrophic Bacteria.

Microbial detection in milk is crucial for food safety and quality, as beneficial and harmful microorganisms can affect consumer health and dairy product integrity. Identifying and quantifying these microorganisms helps prevent contamination and spoilage. The study employs advanced molecular techniques to detect and quantify the genomic DNA for the target hydrolytic enzyme coding genes and based on the multi-align sequence conserved region, specific primer pair, and hydrolysis probes designed using the singleplex qPCR and multiplex qPCR.

How does livelihood capital influence the green production behaviors among professional grain farmers cultivating high-quality rice?

Introduction: As a vital food crop in China, the sustainable production of high-quality rice is essential for ensuring food security and facilitating the green transformation of agriculture. However, the limited adoption of green production technologies for high-quality rice among farmers poses a significant obstacle to the sustainable development of the grain sector. While previous studies have primarily focused on the adoption of green technologies by traditional farmers, there has been a lack of attention on professional grain farmers as a distinct category of agricultural operators.

Oxygen-Generating Biomimetic Nano-Herb System for Synergistic Therapy & Pain Relief in Triple-Negative Breast Cancer via HIF-1α/VEGF Pathway.

Purpose: This study aims to develop an innovative delivery system, (Q+M/MnOx)@Clip, to enhance the bioavailability and therapeutic efficacy of quercetin both in tumor treatment and pain alleviation.

Methods: The (Q+M/MnOx)@Clip system was evaluated to enhance the release of quercetin, investigate its ability to target cancer cells, alleviate tumor hypoxia, and improve the efficacy of chemodynamic therapy (CDT). Tumor hypoxia markers and immune response activation were assessed, along with the impact on pain relief biomarkers.

Role of the SPI1/CDKN2A/p53 signaling pathway in cuproptosis of lung adenocarcinoma cells.

Lung adenocarcinoma (LUAD) is among the most prevalent malignancies worldwide. Cuproptosis, a copper-induced form of cell death, has been identified as a key process in LUAD progression; however, the molecular mechanisms underlying cuproptosis in LUAD and potential therapeutic targets remain unclear. The present study utilized The Cancer Genome Atlas database to retrieve mRNA expression profiles and clinical information of LUAD, identifying 10 candidate genes from differentially expressed genes associated with cuproptosis.

Cinobufagin regulates the microRNA-149-3p/AFF4 axis to affect the proliferation and apoptosis of cisplatin-resistant ovarian cancer cells.

Although cinobufagin has been demonstrated to inhibit the growth of various malignant tumors, its functional role in ovarian cancer remains unclear. In light of this, the present study aims to thoroughly investigate the effects of cinobufagin on ovarian cancer progression and elucidate its underlying molecular mechanisms, thereby providing novel insights into future therapeutic strategies. A2780/DDP, a cisplatin-resistant cell line for ovarian cancer, was treated with gradient doses of cinobufagin.

Utilization of Native CRISPR-Cas9 System for Expression of Glucagon-like Peptide-1 in .

Type 2 diabetes is one of the main causes of cardiovascular diseases, kidney diseases, and visual impairments, posing a global healthcare challenge. The current treatment of this disease, involving glucagon-like peptide-1 (GLP-1), is faced with problems such as frequent injections and plasmid instability. In this study, we used the native clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9 (CRISPR-Cas9) system of to develop a novel, genetically stable, and orally administrable strain expressing human GLP-1.

Integrative omics and multi-cohort identify and biological targets related to sepsis-associated acute respiratory distress syndrome.

Interferon-related genes are involved in antiviral responses, inflammation, and immunity, which are closely related to sepsis-associated acute respiratory distress syndrome (ARDS). We analyzed 1972 participants with genotype data and 681 with gene expression data from the Molecular Epidemiology of ARDS (MEARDS), the Molecular Epidemiology of Sepsis in the ICU (MESSI), and the Molecular Diagnosis and Risk Stratification of Sepsis (MARS) cohorts in a three-step study focusing on sepsis-associated ARDS and sepsis-only controls. First, we identified and validated interferon-related genes associated with sepsis-associated ARDS risk using genetically regulated gene expression (GReX).

ARHGAP11A is a potential prognostic biomarker and therapeutic target for pancreatic adenocarcinoma.

The prognosis of pancreatic adenocarcinoma remains dismal up to now, partly owing to a lack of clinically feasible therapeutic targets. ARHGAP11A, a member of Rho GTPase-activating proteins family, has been reported as a prognostic biomarker and oncogene for multiple cancers. However, the specific effects of ARHGAP11A in pancreatic adenocarcinoma remain obscure.