Transcriptomic analysis of the effect of dietary melatonin on liver immunity in rice field eel (Monopterus albus).

The aim of this study was to investigate the effects of the dietary effects of melatonin, particularly in relation to its ability to regulate the immune system. A total of 360 fish were fed with different concentrations of melatonin (0, 30, 60, 120, and 240 mg/kg) for 70 days. The activities of antioxidant and immune-related enzymes in the liver showed that at a concentration of 120 mg/kg, the activities of superoxide dismutase, catalase, glutathione peroxidase, lysozyme, and alkaline phosphatase were significantly increased, while the content of malondialdehyde and the activities of alanine aminotransferase and aspartate aminotransferase were decreased (p < 0.

Effects of Different Farming Models on Muscle Quality, Intestinal Microbiota Diversity, and Liver Metabolism of Rice Field Eel ().

As consumer demand for quality fish products continues to rise, quality has become a key factor in market competition. Ecological aquaculture research is exploring various farming methods to balance high-quality demand with environmental protection. This study compared three aquaculture models-cage culture (CG), recirculating aquaculture (RAG), and rice-fish co-culture (RG)-by analyzing muscle quality (AOAC, GC-MS), intestinal microbiota (16S rRNA), and liver metabolism (LC-MS) to assess their effects on .

Tire wear particle leachate induces lipid metabolism disorders in Procambarus clarkii, leading to liver inflammation and mitochondrial dysfunction in hepatocytes.

Tire wear particle (TWP) leachate, an emerging pollutant that has gained widespread attention in recent years, poses a serious threat to aquatic ecosystems and particularly to economically important freshwater species. The toxicity of TWP leachate to aquatic organisms underscores its detrimental impact on these ecosystems. In this study, the crayfish Procambarus clarkii was exposed to TWPs at concentrations of 0 %, 0.

Comparative study of trace metal concentration in the diagnosis of category III prostatitis.

Background: Chronic prostatitis (CP) is one of the general diseases in urological practice, with category III prostatitis being particularly prevalent. The trace metal abnormalities might be a primary cause of prostatitis, however, their specific roles in category III prostatitis remain largely unexplored.

Method: In total, 42 expressed prostatic secretion (EPS) samples from IIIa prostatitis patients, 42 from IIIb prostatitis patients, and 45 from controls were collected, along with 42 serum samples from IIIa prostatitis patients, 45 from IIIb prostatitis patients, and 50 from controls for analysis in this study.

Effects of Substituting and as Feed on Growth, Flesh Quality and Intestinal Microbiota of Red Swamp Crayfish ().

This study aimed to evaluate the impact of substituting a portion of feed with (TM) and (EN) on crayfish culture. A total of 270 crayfish (5.1 ± 0.

Effects of dietary melatonin supplementation on growth performance and intestinal health of rice field eel (Monopterus albus).

The objective was to assess the impact of melatonin supplementation on the growth performance and intestinal health of rice field eel, Monopterus albus. Three hundred and sixty fish (28.46 ± 0.

Discharge of pharmaceuticals from a municipal solid waste transfer station: Overlooked influence on the contamination of pharmaceuticals in surface waters.

During the temporal storage of municipal solid wastes (MSWs), pharmaceutically contained in MSWs may percolate into leachates and migrate into receiving waters via surface runoff. However, knowledge of their intra-event variations during the rainfall is quite limited. To fill in this gap, we collected runoff samples in a typical MSW transfer station over the full length of a rainfall event to comprehensively characterize the pharmaceutical contamination profiles.

Diagnostic potential of trace metals concentration in expressed prostatic secretion and serum of patients with category IV prostatitis.

Background: The National Institutes of Health (NIH) category IV prostatitis is a painless prostate gland inflammation, just as its name implies, this type of prostatitis is related with inflammation of the prostate, but most men are not conscious of it. However, category IV prostatitis is fairly common in general populations and reported having indirect relationships with prostate cancer.

Method: We analyzed the concentration of zinc (Zn), copper (Cu), calcium (Ca) and magnesium (Mg) in expressed prostatic secretion (EPS) and serum of patients with category IV prostatitis and healthy controls, investigating the diagnostic potential of different metals in category IV prostatitis using a flame atomic absorption spectrometer (FAAS).

Erratum: Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

[This corrects the article DOI: 10.3892/etm.2015.

Fractional Amplitude of Low-Frequency Fluctuations and Functional Connectivity in Comatose Patients Subjected to Resting-State Functional Magnetic Resonance Imaging.

Objective: The objective of the study is to investigate the changes of fractional amplitude of low-frequency fluctuations (fALFFs) and functional connectivity (FC) in the brain function of comatose patients with resting-state blood-oxygenation-level-dependent functional magnetic resonance imaging (BOLD-fMRI) and to discuss the underlying neurophysiological mechanism of disease.

Materials And Methods: Resting-state BOLD-fMRI scans were conducted on 20 comatose patients and 20 age-matched and gender-matched controls. The differences of fALFF between two groups were analyzed with two-sample -test; significant differences of connectivity between groups were acquired to calculate the FC of the precuneus with other brain regions.

Phosphatidyl inositol 3-kinase (PI3K)-mTOR inhibitor PKI-402 inhibits breast cancer induced osteolysis.

Bone metastasis causes bone pain and pathological bone fracture in breast cancer patients with a serious complication. Previous studies have demonstrated that a novel phosphatidyl inositol 3-kinase (PI3K)-mTOR inhibitor PKI-402 suppressed the growth of breast cancer cells. However, the role of PKI-402 involved in osteolysis induced by breast cancer remains unclear.

Effects of Zinc Combined with Probiotics on Antibiotic-associated Diarrhea Secondary to Childhood Pneumonia.

Aim: The aim of this study was to evaluate the impact of zinc combined with probiotics (Bifico) on antibiotic-associated diarrhea (AAD) secondary to pneumonia.

Methods: A total of 50 patients with AAD secondary to pneumonia were randomly divided into a probiotics group (Bifico) and a combined group (zinc combined with Bifico) and 25 pneumonia patients without AAD as the control group. Serum levels of zinc, diamine oxidase (DAO) activity, D-lactate and intestinal flora [Bifidobacterium, Escherichia coli and Bifidobacterium/E.

A Human Chondrocyte-Derived In Vitro Model of Alcohol-Induced and Steroid-Induced Femoral Head Necrosis.

BACKGROUND Worldwide, femoral head necrosis (FHN), which is also known as avascular necrosis of the femoral head or osteonecrosis of the femoral head, affects millions of people. Excess alcohol intake and steroid use are two common associations with FHN, but their pathogenesis remains unknown. The aim of this study was to develop an in vitro model using human chondrocytes to study alcohol-induced and steroid-induced FHN.

Collagen, agarose, alginate, and Matrigel hydrogels as cell substrates for culture of chondrocytes in vitro: A comparative study.

Autologous chondrocyte implantation (ACI) has emerged as a new approach to cartilage repair through the use of harvested chondrocytes. But the expansion of the chondrocytes from the donor tissue in vitro is restricted by limited cell numbers and dedifferentiation of chondrocytes. In this study, we used four types of hydrogels including agarose, alginate, Matrigel, and collagen type I hydrogels to serve as cell substrates and investigated the effect on proliferation and phenotype maintenance of chondrocytes.

Serum Macroelement and Microelement Concentrations in Patients with Polycystic Ovary Syndrome: a Cross-Sectional Study.

Polycystic ovarian syndrome (PCOS) is one of the most common endocrine diseases. However, its pathogenesis is unclear. We aim to explore the potential relationships between serum macroelements/microelements and PCOS.

Role of (-)-epigallocatechin-3-gallate in the osteogenic differentiation of human bone marrow mesenchymal stem cells: An enhancer or an inducer?

Epidemiological investigations have revealed that the consumption of green tea, which is a rich source of (-)-epigallocatechin-3-gallate (EGCG), is associated with a reduced risk of osteoporosis. A number of in vitro and in vivo studies have also demonstrated that EGCG exerts a significant positive effect on osteogenesis; however, the single effect of EGCG on osteogenic differentiation has been seldom studied. EGCG was hypothesized to function as an enhancer or an inducer.

Relationship of lead and essential elements in whole blood from school-age children in Nanning, China.

Objectives: The aim of this study was to investigate blood lead level and its relationship to essential elements (zinc, copper, iron, calcium and magnesium) in school-age children from Nanning, China.

Methods: A total of 2457 children aged from 6 to 14 years were enrolled in Nanning, China. The levels of lead (Pb), zinc (Zn), copper (Cu), iron (Fe), calcium (Ca) and magnesium (Mg) were determined by an atomic absorption spectrometer.

Impact of partial DAZ1/2 deletion and partial DAZ3/4 deletion on male infertility.

This study aims to investigate the effect of the partial DAZ1/2 deletion and partial DAZ3/4 deletion on male infertility through a comprehensive literature search. All case-control studies related to partial DAZ1/2 and DAZ3/4 deletions and male infertility risk were included in our study. Odd ratios (ORs) and 95% confidence intervals (CIs) were used to assess the strength of the association and its precision, respectively.

Serum quantitative proteomic analysis reveals potential zinc-associated biomarkers for nonbacterial prostatitis.

Background: Prostatitis is one of the most common urological problems afflicting adult men. The etiology and pathogenesis of nonbacterial prostatitis, which accounts for 90-95% of cases, is largely unknown. As serum proteins often indicate the overall pathologic status of patients, we hypothesized that protein biomarkers of prostatitis might be identified by comparing the serum proteomes of patients with and without nonbacterial prostatitis.

Blood lead level and its relationship to essential elements in preschool children from Nanning, China.

Objective: Our study aimed to assess the distribution of blood lead level and its relationship to essential elements in preschool children in an urban area of China.

Design And Methods: A total of 6741 children aged 0- to 6-year-old were recruited. Levels of lead, zinc, copper, iron, calcium, and magnesium in whole blood samples were determined using atomic absorption spectrometry.

Effect of a novel synthesized sulfonamido-based gallate-SZNTC on chondrocytes metabolism in vitro.

The ideal therapeutic agent for treatment of osteoarthritis (OA) should have not only potent anti-inflammatory effect but also favorable biological properties to restore cartilage function. Gallic acid (GA) and its derivatives are anti-inflammatory agents reported to have an effect on OA (Singh et al., 2003) [1].

[Research for the production of recombinant human epidermal growth factor using Samia Cynthia Ricini pupae bioreactor].

The protein production system using a baculovirus Antheraea pernyi nucleopolyhedrovirus (AnpeNPV) as a gene expression vector and its host insect as a natural bioreactor was successful established and its excellent performance in the protein production has been demonstrated. In this paper, the system is used to produce recombinant human epidermal growth factor (rhEGF), which have been widely used in medical and cosmetic treatment. A recombinant AnpehEGF virus has been constructed by replacing the viral polyhedrin gene with the rhEGF gene, and then injected it to Samia cynthia ricini pupae.

Associations of diet and lifestyle with hyperlipidemia for middle-aged and elderly persons among the Guangxi Bai Ku Yao and Han populations.

Background: There are fifty-six ethnic groups in China. Han is the largest group and Bai Ku Yao is the isolated subgroup of the Yao minority. Little is known about the difference in the prevalence of hyperlipidemia between the Han and other minority groups in the rural areas.

Diet, alcohol consumption, and serum lipid levels of the middle-aged and elderly in the Guangxi Bai Ku Yao and Han populations.

Bai Ku Yao is an isolated subgroup of the Yao minority in China. The special customs and cultures including their clothing, intraethnic marriages, corn wine and rum intakes are still completely conserved to the present day. Little is known about the association of diet and alcohol consumption with serum lipid levels in this population.