ACO1 OGDH axis drives mitochondrial immune crosstalk in preeclampsia through systems biology enabling dual target therapy.

Preeclampsia (PE), a devastating pregnancy complication affecting 5% of gravidas worldwide, exhibits poorly characterized connections between mitochondrial dysfunction and immune dysregulation. This study aims to identify integrated mitochondrial-immune biomarkers for preeclampsia by multi-omics analysis of severe PE cohorts, enabling mechanistic insights and diagnostic potential. We developed a novel computational framework integrating multi-omics analysis (GSE10588 transcriptomics), machine learning (LASSO-SVM algorithm), and molecular dynamics simulation.

Development and validation of a machine learning-based survival prediction model for Asian glioblastoma patients using the SEER database and Chinese data.

Glioblastoma is an aggressive, malignant primary brain tumour and the most prevalent histological type of glioma. Our study attempted to investigate the independent predictors of overall survival (OS) and cancer-specific survival (CSS) in Asian patients with glioblastoma and establish predictive models for the OS and CSS of Asian patients with glioblastoma based on the machine learning algorithms. Data from Asian patients with glioblastoma in the SEER database were retrieved and stochastically grouped into a training set (n = 845) and a validation set (n = 362), and patients in our centre were assigned to the test set (n = 172).

Efficacy of Microwave Ablation in the Treatment of Hyperthyroidism With Benign/Malignant Nodules.

Objectives: This study aims to compare and analyze the 3-year efficacy and safety differences of microwave ablation in the treatment of patients with hyperthyroidism with benign nodules/papillary thyroid carcinoma (PTC).

Methods: This retrospective study included 66 patients with hyperthyroidism accompanied by nodules who received microwave ablation treatment from December 2009 to December 2024. A 3-year follow-up was conducted after the operation to monitor indicators such as the complete remission rate of hyperthyroidism and the tumor disappearance rate, and the differences in therapeutic effects between the two groups were analyzed through propensity score matching (PSM).

Efficacy and safety of thermal ablation for the treatment of recurrent and persistent secondary hyperparathyroidism: a multicenter retrospective study.

Objective: To evaluate the long-term efficacy and safety of Ultrasound (US)-guided percutaneous thermal ablation for recurrent or persistent secondary hyperparathyroidism (SHPT) and identify predictors of treatment failure.

Materials And Methods: From January 2014 to July 2023, 102 patients with recurrent or persistent SHPT after parathyroidectomy or ablation received thermal ablation. The median follow-up period was 30 months.

Highly Sensitive Perovskite Nanowire Photodetectors Enabled by Surface State Engineering for Non-Contact Heart Rate Monitoring.

Non-contact heart rate detection is essential in health monitoring, emphasizing the need for highly sensitive photodetectors to ensure accurate measurement. Nanowire photodetectors, benefiting from surface states, exhibit excellent performance. However, the distribution and charge-trapping capability of these surface states are primarily dictated by the intrinsic material properties, limiting tunability.

Bisphenol A in utero induced glutamate and D-serine metabolic dysregulation in the hippocampus of rats and primary cultured astrocytes.

Bisphenol A (BPA) is a widely used synthetic compound that could cause neurobehavioral abnormalities in mammals. Previous studies have suggested that NMDAR may be a potential target of BPA-induced neurotoxicity. However, the impact of exposure to BPA on glutamate (Glu) and D-serine (D-ser) metabolism (key regulators of NMDAR activation) has not been clarified yet.

Development of a machine learning-based model to predict urethral recurrence following radical cystectomy: a multicentre retrospective study and updated meta-analysis.

Urethral recurrence (UR) following radical cystectomy for bladder cancer represents an aggressive disease failure with typically poor survival outcomes. Our study aimed to assess the predictive risk factors for UR, to develop and validate an easy-to-use predictive tool of UR in our region based on machine learning algorithms and to update the meta-analysis of risk factors for UR in combination with our results to improve its accuracy and reliability. Clinicopathological data from the patients who underwent RC between 2010 and 2020 at multiple centres were collected.

Functional screening of somatic mutant events in extranodal natural killer/T-cell lymphoma with adrenal involvement.

Background: Extranodal natural killer/T-cell lymphoma (ENKTL) involving the adrenal glands is extremely rare, and only a few cases have been reported. However, the genetic alterations, clinicopathological features and prognosis of these patients have not yet been fully elucidated.

Methods: Profiling of tumor mutations in ENKTL patients with adrenal involvement was conducted by whole-genome sequencing, and the predisposing genes and driver mutation gene variants were verified through Sanger sequencing.

Clinicopathological features and genetic mutation spectrum of primary anastomosing hemangioma arising from the kidney.

Background: Renal anastomosing hemangioma (RAH) is a rare benign renal tumor, and its clinicopathologic characteristics and genetic mutation spectrum related to its mechanisms of pathogenesis are unclear.

Methods: We carried out whole-genome sequencing (WGS) on RAH samples to explore the genetic mutation spectrum and verified the results by Sanger sequencing. Immunohistochemical analysis was also performed to reveal the histopathological characteristics and the tumor microenvironment components.

Heme oxygenase 1 confers gilteritinib resistance in FLT3-ITD acute myeloid leukemia in a STAT6-dependent manner.

Background: Acute myeloid leukemia (AML) is the most common type of acute leukemia in adults. We previously discovered that heme oxygenase 1 (HO1) is crucial for chemoresistance in AML, but the detailed molecular mechanism of that remains unclear.

Methods: RNA sequencing was conducted to assess transcriptomic changes in three pairs of AML cells after regulating the expression of HO1.

Perioperative and oncological outcomes of single position retroperitoneoscopic radical nephroureterectomy for upper urinary tract urothelial carcinoma.

Radical nephroureterectomy (RNU) is the gold standard therapy for patients with upper urinary tract urothelial carcinoma (UTUC). In this study, we compared the effect of two surgical techniques, single-position complete retroperitoneoscopic radical nephroureterectomy (SCRNU) and laparoscopic nephroureterectomy with open bladder cuff excision (LNOBE), on perioperative and long-term oncological outcomes in UTUC patients. We retrospectively collected information on baseline characteristics, clinicopathological characteristics, surgical approaches, perioperative data, and survival outcomes from 174 patients who underwent RNU for UTUC between January 2018 and June 2023 in our center.

Prioritizing microbial functions over soil quality for enhanced multifunctionality in saline-sodic soil remediation.

Paddy cultivation has become a widely adopted approach for saline-sodic wasteland reclamation, aiming to mitigate the food crisis and enhance soil quality. Nevertheless, the impact of long-term paddy cultivation on the interplay between soil quality, microbial metabolic functions, and soil ecosystem multifunctionality (EMF) remains unclear. Here, we evaluated soil physicochemical properties, the abundance of 132 biomarker functional genes, and soil EMF across a 78-year period of saline-sodic paddy cultivation.

Sesamin targets ClpP which attenuates virulence of S. aureus and protects mice from fatal pneumonia induced by MRSA.

Aims: The aim of this study was to identify sesamin as a Casein hydrolase P (ClpP) inhibitor and to determine whether it could attenuate the virulence of methicillin-resistant Staphylococcus aureus (MRSA).

Methods And Results: Through fluorescence resonance energy transfer screening, a natural compound sesamin demonstrated a significant inhibitory effect on ClpP enzyme activity with an IC50 of 20.62 μg/ml.

One-dimensional hybrid copper halides with high-efficiency photoluminescence as scintillator.

A new one-dimensional hybrid [APCHA]CuI was designed and applied as an X-ray scintillator. It exhibits broad-band green emission with a high PLQY of 74.80% and excellent stability.

Dual roles of CD11bCD33HLA-DRCD14 myeloid-derived suppressor cells with a granulocytic morphology following allogeneic hematopoietic stem cell transplantation: from inflammation promoters to immune suppressors within 90 days.

Introduction: Granulocytic myeloid-derived suppressor cells (G-MDSCs) show fast recovery following allogeneic hematopoietic stem cell transplantation (allo-HSCT) constituting the major part of peripheral blood in the early phase. Although G-MDSCs mediate immune suppression through multiple mechanisms, they may also promote inflammation under specific conditions.

Methods: G-MDSCs were isolated from 82 patients following allo-HSCT within 90 days after allo-HSCT, and their interactions with autologous CD3 T-cells were examined.

Identification of a novel monocyte/macrophage-related gene signature for predicting survival and immune response in acute myeloid leukemia.

Acute myeloid leukemia (AML) is a heterogeneous hematological tumor with poor immunotherapy effect. This study was to develop a monocyte/macrophage-related prognostic risk score (MMrisk) and identify new therapeutic biomarkers for AML. We utilized differentially expressed genes (DEGs) in combination with single-cell RNA sequencing to identify monocyte/macrophage-related genes (MMGs).

Bismuth oxymetallate-modified biochar derived from Euryale ferox husk for efficient removal of Congo red from wastewater: adsorption behavior and mechanisms.

Using Euryale ferox husk as raw material, pristine biochar (EBC), BiMoO-modified biochar (BM-EBC), and BiFeO-modified biochar (BF-EBC) were prepared and employed for decontaminating Congo red (CR) from wastewater. Compared with EBC (217.59 mg/g) and BF-EBC (359.

Value of three-dimensional visualization of preoperative prostatic magnetic resonance imaging based on measurements of anatomical structures in predicting positive surgical margin after radical prostatectomy.

Background: Positive surgical margin (PSM) or apical positive surgical margin (APSM) is an established predictive factor of biochemical recurrence or disease progression in prostate cancer (PCa) patients after radical prostatectomy. Since there are limited usable magnetic resonance imaging (MRI)-based models, we sought to explore the role of three-dimensional (3D) visualization for preoperative MRI in the prediction of PSM or APSM.

Methods: From December 2016 to April 2022, 149 consecutive PCa patients who underwent radical prostatectomy were retrospectively selected from the Second Affiliated Hospital of Dalian Medical University.

Mitochondrial IRG1 traps MCL-1 to induce hepatocyte apoptosis and promote carcinogenesis.

Hepatocarcinogenesis is initiated by repeated hepatocyte death and liver damage, and the underlying mechanisms mediating cell death and the subsequent carcinogenesis remain to be fully investigated. Immunoresponsive gene 1 (IRG1) and its enzymatic metabolite itaconate are known to suppress inflammation in myeloid cells, and its expression in liver parenchymal hepatocytes is currently determined. However, the potential roles of IRG1 in hepatocarcinogenesis are still unknown.

BM-MSCs display altered gene expression profiles in B-cell acute lymphoblastic leukemia niches and exert pro-proliferative effects via overexpression of IFI6.

Background: The tumor microenvironment (TME) is a supportive environment responsible for promoting the growth and proliferation of tumor cells. Current studies have revealed that the bone marrow mesenchymal stem cells (BM-MSCs), a type of crucial stromal cells in the TME, can promote the malignant progression of tumors. However, in the adult B-cell acute lymphoblastic leukemia (B-ALL) microenvironment, it is still uncertain what changes in BM-MSCs are induced by leukemia cells.

Level of humanistic care ability and its influencing factors among nursing aides in long-term care facilities.

Objective: To determine the level and influencing factors of the humanistic care ability (HCA) in nursing aides, thus providing a baseline for its improvement.

Methods: This study investigated 302 nursing aides in six long-term care facilities (LTCFs) in Suzhou between December 2021 and June 2022 by convenience sampling. A descriptive questionnaire and the Caring Ability Inventory were applied in this study.

Directional methanolysis of kitchen waste for the co-production of methyl levulinate and fatty acid methyl esters: Catalytic strategy and machine learning modeling.

To add value to ordinary kitchen waste, heterogeneous acid-base catalytic methanolysis was conducted to produce high-value liquid biofuels, methyl levulinate (ML) and fatty acid methyl esters (FAMEs). Yields of 53.3 % ML and 98.