Effects of Moringa oleifera Seed Protein on the Gel Behavior and In Vitro Digestive Properties of Japonica, Indica, and Glutinous Rice Starch.

This study aimed to investigate the effects of different concentrations of Moringa oleifera seed protein (MOSP) on the water holding capacity (WHC), rheological properties (viscoelasticity), textural properties, microstructure, and in vitro digestibility of japonica rice starch (JRS), indica rice starch (IRS), and glutinous rice starch (GRS). The results showed that MOSP was randomly dispersed in the starch-based gel network structure, and addition of MOSP enhanced the WHC, textural properties, and rheological properties of the gel, so as to obtain a more solid gel network structure. Both moduli (G' and G″) of MOSP-JRS gel reached up to 1.

hijacks mitophagy and lysosomal function to persist in endothelial cells.

Introduction: The direct infection of endothelial cells by (), a keystone periodontal pathogen, has been implicated in the development of atherosclerosis. While non-selective autophagy facilitates its intracellular persistence in endothelial cells, the role of selective autophagy in this process remains unclear. This study investigated whether hijacks mitophagy and lysosomes to persist in endothelial cells.

Differentiation of volatile organic compounds in chili powders of different spiciness levels via E-nose, HS-GC-IMS, and chemometrics.

Introduction: Chili powder is a widely used seasoning whose pungency largely depends on its capsaicin content and volatile compounds.

Methods: This study evaluated the capsaicin levels and pungency of three commercial chili powders labeled light, medium, and strong using a pungency meter. Volatile organic compounds (VOCs) were analyzed via electronic nose and headspace gas chromatography-ion mobility spectrometry (HS-GC-IMS) coupled with multivariate statistical analyses.

Post-Quantum Linkable Hash-Based Ring Signature Scheme for Off-Chain Payments in IoT.

Off-chain payments in the Internet of Things (IoT) enhance the efficiency and scalability of blockchain transactions. However, existing privacy mechanisms face challenges, such as the disclosure of payment channels and transaction traceability. Additionally, the rise of quantum computing threatens traditional public key cryptography, making the development of post-quantum secure methods for privacy protection essential.

Bibliometric analysis of global research trends in pancreatic cancer immunotherapy.

Pancreatic cancer is a highly malignant tumor with poor prognosis and limited treatments. Immunotherapy shows promise in improving outcomes, but bibliometric analyses in this field are scarce. Our work reveals emerging trends, identifies research gaps, and highlights shifts in focus, offering valuable guidance for future research of immunotherapy and clinical practice.

A Post-Quantum Blockchain and Autonomous AI-Enabled Scheme for Secure Healthcare Information Exchange.

Secure healthcare information exchange (HIE) is critical to improving medical services, enabling data interoperability, and ensuring patient privacy. However, the increasing threat posed by quantum computing challenges the reliability of conventional cryptographic mechanisms. To address this, we propose a post-quantum secure healthcare data-sharing scheme that combines the Extended Merkle Signature Scheme (XMSS) and consortium blockchain technology to guarantee the integrity, authenticity, and traceability of electronic medical records (EMRs).

Oxidative stress-induced decreased expression of FABP5 leads to mitochondrial damage and survival disorder of decidual stromal cells in women with recurrent spontaneous abortion.

Background: The survival of decidual stromal cells (DSCs) at the maternal-fetal interface is critical for maintaining pregnancy. Dysfunction of DSCs is a significant contributor to recurrent spontaneous abortion (RSA), though the precise regulatory mechanisms remain elusive. Here, we report a marked downregulation of fatty acid-binding protein 5 (FABP5) in decidual tissues and DSCs from RSA donors, suggesting its potential role in RSA pathogenesis.

Detection of an 8p23.1 Inversion Using High-Resolution Optical Genome Mapping.

Objective: To evaluate the performance of optical genome mapping (OGM) in identifying an inversion located in the short arm of chromosome 8 (8p, 8p23.1), flanked by regions of complex segmental duplication (SD), using the GRCh38 and telomere-to-telomere (T2T) genome references.

Methods: We investigated a couple suspected of carrying the 8p23.

Porphyromonas gingivalis Induces Endothelial Dysfunction Through Sirt3-Dependent CypD Acetylation.

Aims: To investigate how Porphyromonas gingivalis induces endothelial dysfunction, focusing on the regulatory role of Sirtuin 3 (Sirt3) in mitochondrial function.

Methods: Differentially expressed Sirtuin family genes in P. gingivalis-infected human aortic endothelial cells (HAECs) were identified through RNA sequencing and validated by quantitative real-time PCR and Western blot.

Dietary emulsifier Polysorbate 80-induced lipotoxicity promotes intestinal senescence.

Intestinal senescence, often characterized by increased oxidative stress, is linked to gastrointestinal disorders such as inflammatory bowel disease and colorectal cancer. While previous studies have suggested that diets rich in food additives, such as the emulsifier Polysorbate 80 (P80), may influence gut health, the impact of P80 exposure on intestinal senescence remains unclear. This study aimed to explore the effects of P80 on intestinal senescence in a senescence-accelerated mouse prone model.

Maternal high-fat diet disrupts intestinal mucus barrier of offspring by regulating gut immune receptor LRRC19.

Maternal high fat diet (MHFD) increased colitis susceptibility in adulthood. However, the mechanism remains unclear. We sought to explore whether novel gut immune receptor leucine-rich repeat C19 (LRRC19) contributed to the impaired mucus barrier of offspring exposed to MHFD via gut immune response and microbiota.

Molecular mechanism and role of miRNA-155 ribonucleic acid in podocyte apoptosis in lupus nephritis: SOCS1 protein expression regulates JAK/STAT pathway transduction.

This study aimed to explore the specific role of miRNA-155 in the process of podocyte apoptosis within the context of lupus nephritis, as well as to elucidate the underlying molecular mechanisms through which miRNA-155 exerts its effects, particularly focusing on the SOCS1/JAK1-STAT1 signaling pathway. To investigate the direct influence of miRNA-155 on podocyte apoptosis, the researchers employed a cultured glomerular podocyte system, in which they transfected these cells with either mimics of miRNA-155 or inhibitors. The subsequent effects on podocyte apoptosis were carefully monitored.

Long Noncoding RNA SNHG12 Regulates Ischemia/reperfusion (I/R)-mediated Acute Kidney Injury (AKI) Through miR-129-1-3p/Ubiquitin Specific Peptidase 25 axis.

A growing body of evidence suggests the involvement of long noncoding ribose nucleic acids (lncRNAs) in acute kidney injury (AKI). This study focused on the mechanistic role of lncRNA small nucleolar RNA host gene 12 (SNHG12) in ischemia/reperfusion (I/R)-mediated AKI. A model of hypoxia/reoxygenation (H/R) was created using human kidney cells (HK-2).

Gut microbiota in post-acute COVID-19 syndrome: not the end of the story.

The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), has led to major global health concern. However, the focus on immediate effects was assumed as the tip of iceberg due to the symptoms following acute infection, which was defined as post-acute COVID-19 syndrome (PACS). Gut microbiota alterations even after disease resolution and the gastrointestinal symptoms are the key features of PACS.

Comparison of Interactions Between Soy Protein Isolate and Three Folate Molecules: Effect on the Stabilization, Degradation, and Oxidization of Folates and Protein.

This study selected three approved folate sources-folic acid (FA), L-5-methyltetrahydrofolate (MTFA), and calcium 5-methyltetrahydrofolate (CMTFA)-to explore their interaction mechanisms with soy protein isolate (SPI) through spectrofluorometric analysis and molecular docking simulations. We investigated how these interactions influence the structural and physicochemical stability of folates and SPI. Three folates spontaneously bound to SPI, forming complexes, resulting in a decrease of approximately 30 kJ·mol in Gibbs free energy and an association constant (K) of 10 L·mol.

Beneficial Effects of Epigallocatechin Gallate in Preventing Skin Photoaging: A Review.

Skin photoaging, primarily caused by ultraviolet (UV) radiation, leads to skin metabolic disorders, which have adverse psychological and physiological effects on individuals. However, traditional medications for repairing skin photoaging cause side effects. Natural bioactive compounds have been shown to prevent and treat skin photoaging with fewer side effects.

Exploring the Potential of Anthocyanins for Repairing Photoaged Skin: A Comprehensive Review.

Long-term exposure to ultraviolet (UV) rays can result in skin photoaging, which is primarily characterized by dryness, roughness, pigmentation, and a loss of elasticity. However, the clinical drugs commonly employed to treat photoaged skin often induce adverse effects on the skin. Anthocyanins (ACNs) are water-soluble pigments occurring abundantly in various flowers, fruits, vegetables, and grains and exhibiting a range of biological activities.

The clinical application of affected-embryo-based SNP haplotype analysis for patients with de novo pathogenic mutations in PGT-M cycles.

Purpose: In preimplantation genetic testing for monogenic/single gene disorders (PGT-M) cycles, direct detection of the pathogenic mutation combined with indirect haplotype analysis are recommended to achieve accurate diagnosis. However, it poses a challenge to conduct haplotype analysis for patients carried de novo pathogenetic mutations or without no identified haplotype in families. Herein, the strategy of affected-embryo-based haplotype analysis was implemented in clinical practice to provide a convenient, economical and effective way for such patients.

pH-driven fabrication of a caseinate-pectin polyelectrolyte complex as a promising carrier for lutein and zeaxanthin delivery: Microencapsulation, stability, and sustained release properties.

In this study, caseinate-pectin polyelectrolyte complexes and co-solutions were successfully fabricated at pH 3.0 and 7.0, respectively, to encapsulate bioactive molecules.

Decreased formulation pH and protein preheating treatment enhance the interaction, storage stability, and bioaccessibility of caseinate-bound lutein/zeaxanthin.

Heat treatment and pH are crucial factors in the formulation and processing of food and beverages; thus, a thorough understanding of the impact of these factors on the interactions between bioactive constituents and proteins is essential to developing effective protein-based delivery systems. This study explores the influences of pH (ranged from 1.5 to 7.

Non-Covalent Interaction of Folic Acid and 5-Methyltetrahydrofolate with Caseinates Improves the Folates Stability Studied by Multi-Spectroscopic Analysis and Molecular Docking.

Folates, a crucial B-group vitamin, serve as a significant functional food supplement. Nevertheless, considerable obstacles persist in improving folates stability in liquid products. In this study, folic acid (FA) and 5-methyltetrahydrofolate (MTFA), two approved sources of folates, were encapsulated with sodium caseinate (NaCas) to enhance their stability.

Use of GC-IMS and Stoichiometry to Characterize Flavor Volatiles in Different Parts of Lueyang Black Chicken during Slaughtering and Cutting.

Chilled and cut chicken is preferred by consumers for its safeness and readiness to cook. To evaluate the quality characteristics of various chilled chicken products, differences in volatile organic components (VOCs) of six different cut parts (breast, back, leg, heart, liver, and gizzard) of Lueyang black chicken were characterized through gas chromatography-ion mobility spectroscopy (GC-IMS) combined with stoichiometry. A total of 54 peaks in the signal of VOCs were detected by GC-IMS, and 43 VOCs were identified by qualitative analysis.

LncRNA KIFAP3-5:1 inhibits epithelial-mesenchymal transition of renal tubular cell through PRRX1 in diabetic nephropathy.

Long noncoding RNAs play an important role in several pathogenic processes in diabetic nephropathy, but the relationship with epithelial-mesenchymal transition in DN is unclear. Herein, we found that KIFAP3-5:1 expression was significantly down-regulated in DN plasma samples, db/db mouse kidney tissues and high glucose treated renal tubular epithelial cells compared to normal healthy samples and untreated cells. Overexpression of KIFAP3-5:1 improved renal fibrosis in db/db mice and rescued epithelial-mesenchymal transition of high glucose cultured renal tubular epithelial cells.

Non-covalent complexes of lutein/zeaxanthin and whey protein isolate formed at different pH levels: Binding interactions, storage stabilities, and bioaccessibilities.

Lutein (Lut) and zeaxanthin (Zx) are promising healthy food ingredients; however, the low solubilities, stabilities, and bioavailabilities limit their applications in the food and beverage industries. A protein-based complex represents an efficient protective carrier for hydrophobic ligands, and its ligand-binding properties are influenced by the formulation conditions, particularly the pH level. This study explored the effects of various pH values (2.

miR-208a-3p regulated by circUQCRC2 suppresses ischemia/reperfusion-induced acute kidney injury by inhibiting CELF2-mediated tubular epithelial cell apoptosis, inflammation and ferroptosis.

Background : Acute kidney injury (AKI) is a prevalent clinical syndrome with persistent kidney dysfunction. Renal ischemia/reperfusion (I/R) injury is a major cause of AKI. miR-208a-3p overexpression attenuated myocardial I/R injury.