External validation of N2H3 nomogram to predict outcomes in patients with acute ischemic stroke treated by intravenous thrombolysis.

Background: The N2H3 model was evaluated for forecasting the 3-month outcomes for patients experiencing acute ischemic stroke who received intravenous thrombolysis (IVT), in our previous study. The present study aimed to validate the predictive ability of the N2H3 model and to compare its accuracy to the THRIVE-c and START models (both of which are widely employed for prognostic predictions following IVT).

Methods: Our study prospectively enrolled consecutive stroke patients who received IVT from 16 hospitals.

Quantification of brain tissue injury and prediction of prognosis using serum GFAP and UCH-L1: A multicenter prospective cohort study.

Background: It remains unclear whether the serum levels of the brain injury biomarkers (glial fibrillary acidic protein [GFAP] and ubiquitin C-terminal hydrolase-L1 [UCH-L1]) can be used to quantitatively evaluate brain tissue injury and predict prognosis in patients with intravenous thrombolysis (IVT).

Aim: This study investigates the association between serum GFAP and UCH-L1 levels with functional outcomes in patients receiving IVT.

Methods: Patients were prospectively enrolled from 16 hospitals.

TNF receptor-associated factors: promising targets of natural products for the treatment of osteoporosis.

Tumor necrosis factor receptor-associated factors (TRAFs) are crucial intracellular signaling proteins in bone homeostasis. TRAFs mediate pathways associated with bone remodeling, particularly in response to inflammatory stimuli, influencing osteoclast differentiation and function. Dysregulation of TRAF-mediated signaling contributes significantly to osteoporosis, a condition marked by increased bone resorption and fragility.

Photoredox-Catalyzed Direct C(sp)-H Difluoromethylation of Hydrazones with Difluoromethyltriphenylphosphonium Salt via Aminyl Radical/Polar Crossover.

This study describes an efficacious and generally applicable synthetic strategy for the incorporation of biologically and physiologically prominent difluoromethyl entity into synthetically crucial hydrazone scaffolds with bench-stable and easily accessible difluoromethyltriphenylphosphonium bromide. The broad substrate scope, excellent functional group compatibility, feasibility of step and atom economical one-pot synthetic manipulation, and environmentally benign and mild reaction conditions rendered this methodology an efficient tool for the preparation of synthetically and pharmaceutically prominent fluorine-containing imino compounds.

Clinical efficacy and safety of photodynamic therapy combined with tea polyphenols in treating peri-implantitis.

Objective: To evaluate the clinical efficacy and safety of photodynamic therapy (PDT) plus tea polyphenols (TP) in treating peri-implantitis.

Methods: The clinical records of 91 peri-implantitis patients treated at the North China University of Science and Technology Affiliated Hospital and Tangshan Hongci Hospital were analyzed retrospectively. Forty-one patients who received PDT alone were assigned to the control group, while the other 50 patients receiving both PDT and TP were allocated to the study group.

Recurrence of Rosacea Induced by Compound Vitamin Tablets: A Case Report.

We present a case of rosacea recurrence in a 37-year-old woman associated with the intake of compound vitamin tablets during the preconception period, with a Naranjo score of 7. These tablets, commonly used for nutritional supplementation to prevent anemia, contain a variety of vitamins, minerals, and trace elements. Despite their widespread use, reports of such supplements causing rosacea recurrence are rare.

The implications of mutations in multiple genes associated with ethambutol resistance among multidrug-resistant tuberculosis isolates from China.

Objectives: To assess the mutation effects of ethambutol (EMB) resistance-associated genes, including embCAB operon, ubiA, embR, and aftA, on the EMB resistance levels among multidrug-resistant tuberculosis (MDR-TB) isolates from China.

Methods: A total of 159 MDR-TB from China had their EMB MICs quantified, and the sequences of the four ethambutol resistance-associated regions were analyzed. A multivariate regression model was established to evaluate the effects of mutations on EMB resistance.

[Influencing Factors of FeCl Induced Mouse Carotid Artery Thrombosis Model].

Objective: To investigate the factors affecting ferric chloride (FeCl) - induced carotid artery thrombosis model experiment.

Methods: After the common carotid artery was damaged by FeCl, the injured vessels were dissected for fixation, embedding, frozen section, and then processed HE staining. The carotid thrombus area ratio was calculated.

Liriodendrin alleviates myocardial ischemia‑reperfusion injury via partially attenuating apoptosis, inflammation and mitochondria damage in rats.

Myocardial ischemia‑reperfusion (I/R) injury may lead to dysfunction of signaling pathways related to cell apoptosis, inflammation, oxidative stress, and mitochondrial damage. The present study investigated the defensive effect of liriodendrin, as a natural product isolated from , on reperfusion injury in rats and the underlying mechanisms involved in this process. An rat model of I/R constructed by ligation of the left anterior descending artery, as well as an model using H9C2 cells under hypoxic conditions, was established to assess the cardioprotective effects of liriodendrin.

Study on PD-1 inhibitor combined with recombinant human endostatin and chemotherapy followed by IMRT in the treatment of advanced NSCLC.

Although chemotherapy, targeted therapy, and antiangiogenic drugs have become the cornerstones of treatment for advanced non-small-cell carcinoma (NSCLC) in clinical practice, the emergence of immune checkpoint inhibitors (such as PD-1 inhibitors) in recent years has also provided new options for the treatment of NSCLC. To explore whether PD-1 inhibitors combined with recombinant human endostatin and chemotherapy followed by IMRT have a certain curative effect in the treatment of advanced NSCLC. We retrospectively analyzed 47 patients with stage IIIB, IIIC, and IV NSCLC admitted to our hospital from August 2022 to June 2023.

Clinical efficacy and safety of Tinidazole combined with minocycline in treating peri-implantitis.

Objective: To evaluate the effects of tinidazole (TNZ) combined with minocycline (MINO) on therapeutic effectiveness, bone resorption, and inflammation in peri-implantitis (PI).

Methods: This retrospective study included 96 PI patients admitted between January 2023 and February 2024. Patients were divided into a control group (n = 46) treated with MINO and a research group (n = 50) treated with TNZ plus MINO.

Visual codon: a user-friendly Python program for viewing and optimizing gene GC content.

Due to the codon bias of different species, codon optimization is usually carried out in the process of heterologous protein expression. At present, there are a variety of codon optimization tools. However, the optimized sequences may still have high or low points of local guanine and cytosine (GC) content, which is not conducive to the primer design of gene subcloning, and also makes it difficult to perform the experiment of synthesizing the whole gene with DNA fragments by polymerase chain reaction (PCR) reaction.

STT3-mediated aberrant N-glycosylation of CD24 inhibits paclitaxel sensitivity in triple-negative breast cancer.

Paclitaxel is one of the main chemotherapic medicines against triple-negative breast cancer (TNBC) in clinic. However, it has been perplexed by paclitaxel resistance in TNBC patients, resulting in a poor prognosis. Abnormal protein glycosylation is closely related to the occurrence and progression of tumors and malignant phenotypes such as chemotherapy resistance.

Clinical characteristics of patients with variant-associated epilepsy and therapeutic exploration: a case report and literature review.

The gene encodes a transmembrane prolyl 4-hydroxylase, which is responsible for the degradation of hypoxia-inducible transcription factors (HIF) under normoxia. Clinically, biallelic variants have been identified in patients with hypotonia, hypoventilation, intellectual disabilities, dysautonomia, epilepsy, and eye abnormalities (HIDEA syndrome). Seizure was one of the most prominent symptoms.

[Regulation of Reactive Oxygen Species on Platelet Activation and Apoptosis].

Objective: To investigate how reactive oxygen species (ROS) regulates the signal transduction of platelet activation and apoptosis, and to explore the relationship between platelet activation and apoptosis.

Methods: Platelets were directly stimulated with thrombin or pretreated with ROS inhibitor N-acetylcysteine (NAC) before being stimulated with thrombin, and then flow cytometry was used to detect the effects of thrombin and NAC on P-selectin expression, αⅡbβ3 activation, mitochondrial membrane potential depolarization, phosphatidylserine (PS) externalization, ROS expression and platelet aggregation.

Results: Thrombin could induce the production of ROS in platelets in a concentration- and time-dependent manner.

A Python program to merge Sanger sequences: an update.

Gene cloning is an important step in investigating gene structure and function. To verify gene sequence, Sanger sequencing is used, which may produce several overlapping sequencing files that need to be merged before alignment to the target gene sequence is performed. Previously, we reported the Python program to Merge Sanger sequences (https://peerj.

Porto-Sinusoidal Vascular Disease: A New Nomenclature Different from Idiopathic Non-Cirrhotic Portal Hypertension.

Background And Aims: Porto-sinusoidal vascular disease (PSVD) as a novel clinical conception was modified on the basis of idiopathic non-cirrhotic portal hypertension (INCPH). This study aimed to compare the clinical, biochemical histological features and prognosis between the diagnostic criteria for PSVD and that of INCPH.

Methods: A total of 65 patients who underwent liver biopsies were analyzed retrospectively.

Response of CH and NO emissions to the feeding rates in a pond rice-fish co-culture system.

Feeding rate is an important factor influencing the carbon and nitrogen input and greenhouse gas emission from aquaculture systems. However, the quantitative relationship between feeding rates and GHG emissions is still poorly understood. In this study, we conducted a laboratory-scale experiment to examine the impact of feeding rate (0%, 2%, 4%, 6%, and 8%) on the CH and NO emissions from a pond rice-fish co-culture system.

Analysis on epidemiological and drug resistance characteristics of lymph node tuberculosis from Hunan province, China.

Objectives: To investigate the clinical epidemiological and drug resistance (DR) characteristics of lymph node tuberculosis (LNTB) in Hunan Province which locates in South-central China, and to provide scientific clues for effective prevention and treatment of LNTB.

Methods: We retrospectively collected LNTB patients with culture positive at Hunan Chest Hospital, the biggest TB reference hospital in South-central China, from January 2013 to December 2021. The multiple demographic, clinical and drug susceptibility data of patients were collected from the hospital's electronic patient records.

Radiofrequency ablation combined with transcatheter arterial chemoembolization for recurrent liver cancer.

Background: The recurrence rate of liver cancer after surgery is high. Radiofrequency ablation (RFA) combined with transcatheter arterial chemoembolization (TACE) is an effective treatment for liver cancer; however, its efficacy in recurrent liver cancer remains unclear.

Aim: To investigate the clinical effect of TACE combined with RFA in the treatment of recurrent liver cancer.

Catalase catalyzed tannic acid-Fe network coating: A theranostic strategy for intestinal barrier restoration.

Epithelial barrier impairment of intestinal inflammation leads to the leakage of bacteria, antigens and consequent persistent immune imbalance. Restoring the barrier function holds promise for management of intestinal inflammation, while the theragnostic strategies are limited. In this study, we developed a novel coating by catalase (CAT)-catalyzed polymerization of tannic acid (TA) and combined chelation network with Fe.