Analysis of m7G-related signatures in the tumor immune microenvironment and identification of clinical prognostic regulators in ovarian cancer.

Ovarian cancer (OV) is the most lethal gynecological malignancy in the world. At present, the effect of m7G modification-related genes on the development of ovarian cancer remains unclear. We performed consensus clustering of ovarian cancer samples based on the expression of 24 m7G modification-related genes, and obtained 2 subtypes.

Lower serum GPX4 and GSH/GSSG ratio are associated with poor prognosis in severe community-acquired pneumonia.

Objectives: Disruption of redox homeostasis and ferroptosis have been increasingly implicated in the pathogenesis of inflammatory lung injury. Glutathione peroxidase 4 (GPX4) and the ratio of reduced to oxidized glutathione (GSH/GSSG) are key regulators in this process. However, the clinical significance of ferroptotic factors in severe community-acquired pneumonia (CAP) is still lacking.

Prostate Cancer Cells Secrete PD-1 in Exosomes to Enhance Myeloid-Derived Suppressor Cell Activity and Promote Tumor Immune Evasion.

PD-1 restrains effective killing of cancer cells by the immune system and is predominantly located on the surface of T cells or other immune cells. However, cancer cells also express PD-1 to varying degrees, which is commonly associated with a poor prognosis. Here, we investigated the regulation and function of PD-1 expression in prostate cancer (PCa) and revealed the impact on the tumor microenvironment.

Construction of mitochondrial signature (MS) for the prognosis of ovarian cancer.

Background: Ovarian cancer (OV) continues to be the most lethal type of gynecological cancer with a poor prognosis. During tumorigenesis and cancer advancement, mitochondria are key players in energy metabolism. This study focuses on exploring the mitochondria-related genes for the prognosis of OV.

Multiple Host-Guest Interactions in Metal-Organic Frameworks Constructed by Inverted Calix[4]arenes.

Calixarenes are versatile macrocyclic compounds featuring unique basket-like cavities that are capable of encapsulating guest molecules selectively. Yet, their application potentials as the building blocks for supramolecular assemblies have not been thoroughly explored. In this work, a carboxyl-modified azocalix[4]arene (CAC4A) is selected as the organic ligand to construct metal-organic frameworks (MOFs), an emerging class of porous materials based on coordination units.

High-throughput LDI MS technology decodes the distinct metabolic landscape of prostate cancer in a large-scale cohort.

Background: Prostate cancer (PCa) remains a leading global malignancy, yet current diagnostic reliance on prostate-specific antigen (PSA) testing is limited by suboptimal sensitivity and specificity for early-stage detection. The present study aims to establish an effective high-throughput screening technique for accurate PCa diagnosis.

Methods: A large-scale cohort of 28,892 subjects was considered for inclusion in this study, and 1133 volunteers were finally selected, including 600 healthy controls, 160 patients diagnosed with other diseases of urinary system, 89 patients diagnosed with benign prostate hyperplasia (BPH), and 284 PCa patients.

Enhancing ovarian cancer prognosis with an artificial intelligence-derived model: Multi-omics integration and therapeutic implications.

Background: Gynecological malignancies, particularly ovarian cancer, pose a formidable challenge to women's wellbeing, as evidenced by the global incidence and mortality rates, emphasizing the pressing need for advanced diagnostic and treatment modalities. The heterogeneity of ovarian cancer poses challenges for traditional therapeutic approaches, necessitating the exploration of novel, precision medicine techniques.

Methods: This study leveraged multi-dataset analysis to construct and validate an Artificial Intelligence-Derived Prognostic Index (AIDPI) for ovarian cancer.

Dual locking plate fixation, PRP-augmented autologous bone grafting, and bioactive core construction for femoral fracture nonunion: a retrospective study of 52 cases.

Background: Femoral nonunion remains a challenging orthopedic condition. This study evaluates a combined protocol integrating biomechanical stabilization (dual locking plate fixation) and maximal biological stimulation (PRP-augmented autologous bone grafting with bioactive core construction) to optimize bone healing.

Methods: A retrospective analysis included 52 femoral nonunion patients treated at a tertiary trauma center (2020-2024).

Targeting MAN1B1 potently enhances bladder cancer antitumor immunity via deglycosylation of CD47.

Background: Only a few bladder cancer patients benefit from anti-programmed cell death protein 1/programmed cell death ligand 1 immunotherapy. The cluster of differentiation 47 (CD47) plays an important role in tumor immune evasion. CD47 is a highly glycosylated protein, however, the mechanisms governing CD47 glycosylation and its potential role in immunosuppression are unclear.

Multi-Mode Mechanochromic Responses from Cholesteric Liquid Crystal Elastomer Tubes of Uniform Sheath.

Materials that exhibit varied optical responses to different modes of mechanical stimuli are attractive for complex sensing and adaptive functionalities. However, most mechanochromic materials are fabricated from films or fibers with limited actuation modes. Here, hollow tubes of a symmetric sheath are created using cholesteric liquid crystal elastomers (CLCEs) at the sub-millimeter scale.

Spatiotemporal assessment of renewable adequacy during diverse extreme weather events in China.

China has one of the world's largest wind and solar energy capacity worldwide; however, frequent extreme weather events remain major challenges to power systems. This study provides a nuanced examination of power balance issues on seven extreme weather types that occurred from 2020 to 2050. Utilizing an advanced metric for renewable adequacy and a comprehensive dataset comprising 1,089,891 alert records from 2843 counties, the results indicate that both adequacy surplus and deficit coexist in power systems under extreme weather conditions.

Case Report: Based on the diamond theory, successful treatment of stubborn tibial nonunion after six surgeries using PRP-augmented therapy: a case report and literature review.

Background: The treatment of stubborn tibial nonunion remains a significant challenge. This case report describes a patient who underwent six surgeries and was treated using a stepwise surgical approach based on the diamond theory. The application of platelet-rich plasma (PRP) enhanced autologous iliac bone grafting combined with locked compression plate fixation ultimately achieved successful healing.

Factors Influencing the Mental Health of First-Year College Students: Evidence from Digital Records of Daily Behaviors.

The mental health of college students, particularly first-year students, deserves significant attention. As they transition from high school to college, they experience substantial changes not only in their learning behaviors but also in their daily behaviors, which may pose mental health challenges. However, existing studies exploring the factors influencing college students' mental health still have limitations in the selection of predictor variables and behavior assessment.

Based on the diamond concept, application of platelet-rich plasma in the treatment of aseptic femoral shaft nonunion: A retrospective controlled study on 66 patients.

Background: Biologics, particularly platelet-rich plasma (PRP), have gained attention for promoting bone healing. This study assesses the efficacy of PRP-enhanced therapy in treating aseptic femoral shaft nonunion.

Methods: This retrospective study analyzed patients who underwent femoral fracture nonunion revision surgery at a high-level trauma center between January 2021 and April 2024.

Inherently anti-metastatic peptide hydrogels for sonodynamic-amplified ferroptosis in cancer therapy.

Cancer metastasis remains a significant challenge in oncology, prompting the exploration of innovative biomaterials to enhance treatment efficacy. While many hydrogels only serve as passive carriers, this study presents two novel self-assembling peptides, and , which form supramolecular hydrogels with intrinsic anti-metastatic properties. We demonstrate a correlation between the nanofibrous morphology of these peptides and their enhanced anti-metastatic activity, mediated by disruption of F-actin organization and impacting pathways related to cancer cell adhesion and actin filament dynamics.

Event-Based Video Reconstruction With Deep Spatial-Frequency Unfolding Network.

Current event-based video reconstruction methods, limited to the spatial domain, face challenges in decoupling brightness and structural information, leading to exposure distortion, and in efficiently acquiring non-local information without relying on computationally expensive Transformer models. To address these issues, we propose the Deep Spatial-Frequency Unfolding Reconstruction Network (DSFURNet), which explores and utilizes knowledge in the frequency domain for event-based video reconstruction. Specifically, we construct a variational model and propose three regularization terms: a brightness regularization term approximated by Fourier amplitudes, a structural regularization term approximated by Fourier phases, and an initialization regularization term that converts event representations into initial video frames.

The membrane-proximal external region of human immunodeficiency virus (HIV-1) envelope glycoprotein trimers in A18-lipid nanodiscs.

During human immunodeficiency virus (HIV-1) entry, the metastable pretriggered envelope glycoprotein (Env) trimer ((gp120/gp41)) opens asymmetrically. We present cryo-EM structures of cleaved asymmetric Env trimers in amphipol-lipid nanodiscs. The gp41 membrane-proximal external region (MPER) could be traced in Env protomers that remained close to the nanodisc despite Env tilting.

Rapaprotin is Activated by an Endopeptidase to Disassemble 26S Proteasome.

The 19S regulatory particle (RP) associates with the 20S core particle (CP) to form the 26S proteasome, an evolutionarily conserved holoenzyme that plays key roles in both physiological and pathological processes. Proteasome inhibitors that target the catalytic subunits within the 20S have proven to be valuable research tools and therapeutics for various cancers. Herein we report the discovery of rapaprotin, a 26S proteasome assembly inhibitor from our natural product-inspired hybrid macrocycle rapafucin library.

Potential Biomarkers of Post-stroke Cognitive Impairment in Chinese Population: a Systematic Review and Meta-Analysis.

It is still controversial whether peripheral blood biomarkers have the potential to be diagnostic, prognostic, and therapeutic targets for post-stroke cognitive impairment (PSCI). All studies reporting the correlation between peripheral blood biomarkers and PSCI in Chinese acute ischemic stroke (AIS) patients were screened in eight databases and meta-analyses were performed to explore their predictive value for PSCI. The results showed that the levels of C-reactive protein (CRP), fasting blood glucose (FBG), homocysteine (Hcy), and cystatin C (CysC) were significantly higher in the PSCI group than in the post-stroke cognitive impairment no dementia (PSNCI) group.

After-School Behaviors, Self-Management, and Parental Involvement as Predictors of Academic Achievement in Adolescents.

How adolescents' after-school behaviors, self-management, and parental involvement are associated with their academic success and personal development has drawn growing attention. This study systematically examined how these three factors predict academic achievement. Data were collected from 353 seventh-grade students through a self-designed daily behavior log sheet and self-management app, and multiple linear regression analysis was conducted to identify key predictors.

Indirect Construction of Chiral Metal-Organic Frameworks for Enantioselective Luminescence Sensing.

ConspectusChiral metal-organic frameworks (MOFs) are promising candidates as luminescent sensing materials for chiral species, which are essential components in modern industries, pharmaceuticals, and biological processes. The discrimination of enantiomers with highly similar physical and chemical properties is crucial because they are often present concurrently in the same system but may feature distinct effects on living matters. While the rapid and precise sensing capabilities of chiral MOFs outshine traditional detection methods for chiral species in daily life, chemical production, and the natural environment, it requires well-matched chemical and electronic structures between MOFs and chiral species.

Single-Cell RNA Sequencing Reveals the Cellular Origin and Evolution of Small-Cell Neuroendocrine Carcinoma of the Cervix.

Small-cell neuroendocrine cancer (SCNEC) of the uterine cervix is an exceedingly rare, highly aggressive tumor with an extremely poor prognosis. The cellular heterogeneity, origin, and tumorigenesis trajectories of SCNEC of the cervix remain largely unclear. We performed single-cell RNA sequencing and whole-exome sequencing on tumor tissues and adjacent normal cervical tissues from two patients diagnosed with SCNEC of the cervix.