ChREBP-Mediated Choline Deprivation and Chemokine Secretion Shape Tumor-Associated Macrophages to Promote Immune Evasion.

Tumor metabolic reprogramming has been recognized as a critical determinant in tumor development and cancer immunotherapy response. Aberrant choline metabolism is emerging as a defining hallmark of cancer. Here, we found that carbohydrate responsive element binding protein (ChREBP)-mediated choline deprivation induced tumor-associated macrophage (TAM) reprogramming and maintained an immunosuppressive tumor microenvironment (TME).

Effect of Intravenous Lidocaine on Postoperative Cognitive Dysfunction in Patients Undergoing Laparoscopic Colorectal Surgery: A Two-Center, Randomized, Double-Blind Controlled Trial.

Background: In patients undergoing gastrointestinal surgery, enhancing perioperative cognitive function and facilitating expedited postoperative recovery are critical components for achieving swift rehabilitation. Intravenous administration of lidocaine has been shown to mitigate the perioperative inflammatory response in surgical patients; however, its influence on postoperative cognitive performance remains unassessed. Consequently, this study was conducted to investigate the impact of intravenous lidocaine on postoperative cognitive function in participants undergoing laparoscopic surgery for colorectal cancer.

Homozygous variant in JARID2 causes female infertility characterized by compromised blastulation efficiency.

Background: Impaired embryonic developmental competence is a critical determinant of assisted reproductive technology (ART) failure, yet current genetic diagnostics primarily address complete early embryonic arrest, leaving partial developmental defects unexplained, such as compromised blastulation efficiency (CBE). Jumonji and AT-rich interaction domain containing 2 (JARID2), encoding a chromatin-modifying factor essential for histone methylation regulation, emerges as a novel candidate in this context.

Results: We identified a homozygous JARID2 missense variant (c.

Case report of non-gene editing CD7 CAR T cell therapy in CD7 Sézary syndrome: preclinical validation and first-in-human use.

Introduction: Sézary syndrome (SS) is a leukemic form of cutaneous T cell lymphoma (CTCL), distinguished from mycosis fungoides by the presence of cancerous lymphocytes in the blood and often bears very poor prognoses. SS treatment is palliative, and thus novel therapies are needed. The CD7 surface antigen is highly expressed and confined to the surface of T cells, therefore when present, serves as a promising target for immunotherapy.

Urinary Bladder Matrix as a Guide Bone Regeneration Barrier Membrane for Inhibiting Cell Invasion and Promoting Bone Formation.

Natural resorbable collagen membranes are widely used in guided bone regeneration (GBR) in oral implantology and prosthodontics. However, the rapid degradation and inadequate mechanical properties raise concerns about potentially compromising the ultimate bone regeneration efficacy. In this study, we developed a novel GBR membrane by coating a urinary bladder matrix (UBM) onto small intestinal submucosa (SIS), a commonly used acellular material, to improve the cell-barrier and bone-regeneration performances.

Hybrid low-complexity baud-rate sampling and adaptive equalization technique for 400 G/800 G coherent metropolitan area networks.

Baud-rate sampling and adaptive equalization (BSAE) techniques digitize and adaptively equalize received optical signals at the baud-rate and thus can concurrently reduce ADC and equalization power consumption compared with oversampling and fractionally-spaced adaptive equalization (OFAE) techniques operating at a higher sampling rate. Although suitable for short-reach intensity modulation-direct detection (IM-DD) based access networks or intra-datacenter networks, the BSAE techniques are generally regarded as not suitable for metropolitan area networks (MANs), owing to their limited capability to compensate for the large chromatic dispersion (CD) and in-phase/quadrature (IQ) skew distortions encountered in MANs. This constraint renders them inferior to the OFAE techniques able to compensate for arbitrary CD and IQ skew distortions.

Quantitative Phosphoproteomic Analysis of Testes from -Deficient Mice Highlights the Significance of Calmodulin Signaling in Spermiogenesis.

Calmodulin (CaM) plays a crucial role in sperm function. Studies have reported that proteins containing the IQ motif interact with CaM, subsequently engaging with downstream target proteins known as calmodulin-binding proteins (CaMBPs). However, no relevant reports have been published detailing which CaMBPs exist and the mechanisms by which they are regulated.

OTUD3-mediated stabilization of SLC7A11 drives sunitinib resistance by suppressing ferroptosis in clear cell renal cell carcinoma.

Clear cell renal cell carcinoma (ccRCC), as the main type of RCC was treated with tyrosine kinase inhibitor (TKI) at the late stage. While drug resistance is the main challenge for TKIs like sunitinib, and the underline mechanisms remain unclear. Here, we found that OTUD3 is over-expressed in ccRCC and promotes sunitinib resistance in tumor cells.

miRNA-125b-5p-rich exosomes derived from fibro-adipogenic progenitors promoting muscle regeneration through inducing pro-regenerative macrophages.

It is still unclear the role of exosomes derived from fibro-adipogenic progenitors (FAP) on macrophage polarization and muscle repair. In this study, the effects of exosomes collected from primary FAPs isolated from acute injured muscles at different timepoints on macrophage polarization were compared. We found exosomes derived from FAPs isolated at 10 (days post injury, DPI) can effectively induce M2 macrophage polarization both in vitro and in vivo.

Wide-range human physiological signal acquisition with carbonized composite nanofibers.

Flexible pressure sensors provide a powerful platform for information acquisition from the surrounding environment and individual body with bendable, stretchable, conformal and biocompatible properties. However, current pressure sensors with high sensitivity often come at a high cost and have a limited pressure measurement range, which remarkably constrains their adaptability to the diverse physiological activities. Herein, we developed carbonized polyacrylonitrile (PAN) nanofibers (CPNs) by combining electrospinning with high-temperature carbonization.

Perioperative management of total hip arthroplasty in hemophilia A following gene therapy.

Not available.

The niacin receptor HCAR2 prevents sleep deprivation-induced cognitive impairment by inhibiting microglia-mediated neuroinflammation in mice.

Sleep loss has become a common occurrence nowadays, posing significant concerns to public health by increasing risks of various diseases. Studies have shown that sleep loss can cause neuroinflammation and cognitive decline, yet its exact mechanisms and reliable prevention strategies remain unclear. Hydroxycarboxylic acid receptor 2 (HCAR2) is a G protein-coupled receptor expressed in various immune cells, and its activation shows beneficial anti-inflammatory effects in some peripheral and central inflammatory diseases.

A UGW communication method using Barker encoding and improved PPM for rail structural health monitoring.

Given the high communication cost in the existing rail structural health monitoring (SHM) system UGW-based and no GPRS signals in the tunnel, a novel communication method in the rail based on Barker encoding and improved pulse position modulation (IPPM) is proposed in this work. Firstly, to overcome the low signal-to-noise ratio (SNR) of UGW signals resulting from dispersion and multimodal properties, Barker codes are involved in encoding excitation signals for piezoelectric ultrasonic transducers. Besides, an IPPM method is presented to diminish the multipath effect, improving communication robustness.

Clinical changes of cardiac function from high altitude returning to plain.

The clinical feature of cardiac functions in the areas of high altitude and plain still remains unclear. The present study intends to explore the changes of cardiac enzymes and echocardiography in population from high altitude to plain. Medical examinations were performed in a group of young male migrants who worked at high altitude of 4,888 or 5,418 m and returned to the plain of 400 m.

Preimplantation genetic testing for facioscapulohumeral dystrophy caused by contractions of 4q35 D4Z4 repeats.

Research Question: Preimplantation genetic testing for monogenic diseases (PGT-M) is based on mutation testing combined with linkage analysis; so, how should PGT-M for patients with facioscapulohumeral dystrophy type 1 (FSHD1) be conducted when direct mutation testing is unavailable and specific linked markers are lacking?

Design: Patients with a definite FSHD1 diagnosis who intended to prevent the transmission of the condition to their children were recruited in the Reproductive and Genetic Hospital of CITIC-Xiangya, China between May 2021 and December 2023. Familial genetic risk was evaluated using Bionano, 4q haplotyping and whole-exome sequencing. Linkage markers near the causative 4q35 D4Z4 repeats were identified using targeted sequencing and MicroSeq.

Dual Pathway Enables High-Level Production of Very Long-Chain Fatty Alcohol in .

Very long-chain fatty alcohols (VLCFOHs) are crucial components for the production of lubricants, cleaners, and cosmetics. Microbial production of these fatty alcohols has emerged as a more sustainable and potentially cost-effective alternative to traditional manufacturing methods; however, the production levels require significant optimization. Oleaginous microorganisms have received special attention for their ability to produce fatty acid derivatives due to their high lipid content.

Activation of the pentose phosphate pathway mitigates platelet storage lesions and improves platelet preservation quality.

Background: Platelet storage lesions (PSLs) impact platelet lifespan and transfusion quality. This study explores platelet storage metabolism for potential interventions.

Methods: Samples were collected on days 1, 3, and 5 for untargeted metabolomics analysis to identify metabolites that differ with storage time and potential metabolic pathways.

Self-measurement and calibration of amplitude-frequency response for coherent optical transmitters with limited DAC resolution.

Precise measurement and calibration of the amplitude-frequency response (AFR) of coherent optical transmitters (Tx) are paramount for generating high-quality signals, especially when the transmitter has insufficient bandwidth. The previously proposed self-measurement and calibration (SMC) method based on multi-tone signals (MTS) is cost-effective, fast, and convenient. However, this method exhibits a compromised AFR measurement precision in high-frequency regions, especially when the digital-to-analog converters (DAC) of the Tx have a limited resolution.

Study of shrimp tropomyosin on in vitro digestive stability, and allergenicity from mice model by enzyme-mediated glycosylation.

The study aimed to investigate the digestive stability and allergenicity of shrimp tropomyosin (TM) by transglutaminase (TG)-catalyzed glycosylation (TGcG). The structure of samples was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), circular dichroism (CD), fluorescence, and surface hydrophobicity. The digestive stability was determined by simulated gastrointestinal fluid.

Thrombomodulin Arg403Lys mutation impairs protein C activation and increases thrombosis risk.

Thrombomodulin (TM) is an important regulator in various physiological processes, including coagulation, fibrinolysis and inflammation. TM exerts its cofactor activities for protein C (PC) activation and thrombin-activatable fibrinolysis inhibitor (TAFI) activation through its binding with thrombin. Numerous studies have reported that mutations of TM are associated with thromboembolic diseases.

Novel Resolvin D1-Loaded Biologics as an Advanced Approach for Inflammation Control and Tissue Regeneration: Preparation and Characterization.

Constant inflammation can be a detrimental response in bone regeneration. To regulate of the inflammatory response and synchronically promote rapid tissue regeneration is a vital clinical challenge. The urinary bladder matrix (UBM) and small intestinal submucosa (SIS) composite are commonly used extracellular matrix (ECM) materials.

Comprehensive quantitative evaluation and mechanism analysis of influencing factors on yield and quality of cultivated Gastrodia elata Blume.

Gastrodia elata Blume (G. elata Bl.) is a dual-purpose herb for medicine and food.

Advance on Blast-Induced Traumatic Brain Injury.

Blast-induced traumatic brain injury (bTBI) is an injury to brain tissue caused by the blast shock wave of an explosive, which is the leading cause of death and disability among military personnel during wartime. By establishing animal models of bTBI that simulate different degrees and types of injuries, it is possible to clarify the neuropathological changes including molecular and cellular mechanisms. The aim of this review is to summarize the establishment of animal models of bTBI, the mechanism, diagnosis, treatment and brain-related diseases of bTBI, which will provide a basis for improving the understanding of bTBI.