The Role of p53 in Adipocyte Differentiation and Lipid Metabolism in Obese Mice via Transcriptional Regulation of Lgals3.

Objective: This study investigates the regulatory role of p53 on Lgals3 expression and its impact on preadipocyte differentiation, fatty acid synthesis, and oxidation in obesity.

Methods: Bioinformatics analysis of six obesity-related microarray datasets and single-cell RNA sequencing (scRNA-seq) data identified Lgals3 as a key obesity-associated gene. A high-fat diet (HF) mouse model was established to evaluate obesity-related phenotypes, including body weight, hepatic Lgals3 expression, adipose tissue pathology, blood lipid profiles, and glucose tolerance.

RePaint High-Density Surface Electromyography Signal Using Denoising Diffusion Probabilistic Model.

Objective: High-density surface electromyography (HD-sEMG) has emerged as a powerful tool for myoelectric control and activation pattern analysis. However, signal loss due to poor electrode contact and channel corruption remains a significant challenge, limiting the reliability and practical applications of HD-sEMG signals. Conventional interpolation methods fail to effectively reconstruct corrupted signals, especially when multiple adjacent channels are affected.

Recent advances in universal chimeric antigen receptor T cell therapy.

While chimeric antigen receptor (CAR) T cell therapy is highly effective for hematological malignancies, its widespread use is limited by complex, patient-specific manufacturing. Universal CAR-T (UCAR-T) cells, derived from allogeneic donors, offer a potential "off-the-shelf" solution. However, their clinical translation hinges on overcoming two key immunological barriers: graft-versus-host disease (GvHD) and host-versus-graft rejection (HvGR), which compromise safety and therapeutic persistence.

Integrated multi-omics characterization across clinically relevant subgroups of long COVID.

When SARS-CoV-2 became regional epidemics, a substantial number of patients suffered from post-acute sequelae of COVID-19 (PASC, aka long COVID). Exploring the pathogenesis and especially the heterogenicity features of long COVID subgroups is of paramount importance for understanding its etiology. In this study, through integrative multi-omics analyses encompassing transcriptomics, proteomics, and metabolomics, long COVID patients exhibited overall elevated MAPK pathway activation, while patients who have recovered from long COVID showed down-regulation of this response.

Molecular characterization of zinc metalloproteinase Nas-14 from Trichinella spiralis and its participation in intestinal invasion.

Astacins, a family of zinc metalloproteinases, are involved in invasion and tissue migration processes in a variety of parasites. An astacin-like proteinases have been detected in the excretory-secretory products (ESPs) of Trichinella spiralis (T. spiralis), zinc metalloproteinase Nas-14 (TsNas14), but its function in T.

Distinct STING-IRF3 activation determines IL-35 Breg differentiation in rodent malaria.

Augmented regulatory B cell (Breg) responses are commonly observed in malaria; however, the specific parasite components and Breg subtypes involved remain unclear. In this study, we investigated C57BL/6 mice infected with Plasmodium berghei ANKA, which induces cerebral malaria pathology, in comparison to P. yoelii YM, which does not.

Dual Role of Host Toll-Like Receptor 3 in Parasitic Diseases.

Parasitic diseases, caused by a diverse array of parasites, remain a substantial threat to global health. Toll-like receptor 3 (TLR3) represents a pivotal element in the innate immune system, distinguished by an ability to signal via the TIR-domain-containing adapter-inducing interferon-β (TRIF)-dependent pathway upon detecting pathogen-derived double-stranded RNA (dsRNA), exosomal RNA (exoRNA), and long non-coding RNA (lncRNA). Predominantly localized on endosomal membranes, TLR3 is extensively expressed in neurons, immune cells, fibroblasts, and epithelial cells.

Neural Signals-Based Respiratory Motion Tracking: A Surface Electromyography Study.

Purpose: Neural signals-based respiratory motion tracking offers a potential solution to the system latency issue of medical linear accelerators in respiratory motion tracking radiation therapy. However, decoding respiratory-related neural signals from scalp electroencephalography in real-time is challenging. Herein we propose a clinically applicable neural signals-based respiratory motion tracking approach using surface electromyography (sEMG).

Functionalized Trichinella spiralis anti-tumor protein with polydopamine for hepatocellular carcinoma phototherapy and immunotherapy.

As a novel anti-tumor protein recently identified from Trichinella spiralis (T. spiralis) via cDNA display library screening, T. spiralis anti-tumor protein (TspATP) exhibits remarkable potential for inducing immunogenic cell death (ICD).

Surface Gene Mutations of Hepatitis B Virus and Related Pathogenic Mechanisms: A Narrative Review.

Liver cancer has high incidence and mortality rates worldwide, with hepatocellular carcinoma (HCC) being the main histological subtype, accounting for 90% of primary liver cancers. The high mutation rate of viruses combined with endoplasmic reticulum stress may lead to the occurrence of cancer. Hepatitis B virus (HBV) infection is one of the most important pathogenic factors of HCC.

Predictive Value of Hepatitis B Core-Related Antigen for Multiple Recurrence Outcomes After Treatment Cessation in Chronic Hepatitis B: A Meta-Analysis Study.

Background: Hepatitis B core-related antigen (HBcrAg), a novel serum biomarker reflecting the activity of intrahepatic covalently closed circular DNA (cccDNA), has generated conflicting evidence regarding its clinical utility for predicting post-antiviral therapy relapse in chronic hepatitis B (CHB) patients.

Methods: We systematically analyzed 13 studies (15 cohorts, = 1529 patients) from PubMed, Web of Science, Wanfang, and CNKI (through April 2025). A bivariate model evaluated HBcrAg's predictive performance for relapse outcomes, including virological relapse, clinical relapse, and hepatitis flares.

LW-AFC Protects Against Neurological and Neuropsychiatric Effects of SARS-CoV-2 Spike Protein in Mice.

Numerous clinical studies show that besides respiratory symptoms, COVID-19 patients frequently undergo neurological and neuropsychiatric problems. However, effective treatments for these problems remain insufficient. LW-AFC, derived from traditional Chinese medicine Liuwei Dihuang decoction, is effective in improving cognitive and mental dysfunctions in many animal models.

Effectiveness of intramedullary nails in Tibiotalocalcaneal arthrodesis for Charcot neuroarthropathy: a systematic review and meta-analysis.

Objective: To systematically evaluate the efficacy and safety of intramedullary nails (IMNs) in tibial-talocalcaneal arthrodesis (TTCA) for treating Charcot neuroarthropathy (CN).

Methods: A comprehensive search for relevant literature was conducted in the PubMed, Embase, Cochrane Library, Web of Science, Scopus and SinoMed databases, covering studies from 2014 to October 30, 2024. The inclusion criteria were based on the PICOS framework: the study population consisted of CN patients, the intervention was TTCA with IMNs, and the outcomes assessed included bone union rate, complication rate, and limb salvage rate.

Optimal dose of resistance training to improve handgrip strength in older adults with sarcopenia: a systematic review and Bayesian model-based network meta-analysis.

Background: Sarcopenia is prevalent in older adults and affects their quality of life and overall health, low handgrip strength is one of the main manifestations of sarcopenia. Resistance training is an effective intervention for improving muscle strength in older adults, but the optimal dose of resistance training remains unclear. Therefore, the aim of this meta-analysis was to investigate the dose-response relationship between different doses of resistance training and grip strength in older adults.

TDP-43 pathology induces CD8 T cell activation through cryptic epitope recognition.

Aggregation and nuclear depletion of the RNA binding protein TDP-43 are the crucial pathological features of amyotrophic lateral sclerosis (ALS) and inclusion body myositis (IBM), two degenerative diseases of the CNS and muscle. The loss of TDP-43 nuclear function results in the aberrant inclusion of cryptic exons in mRNA transcripts, leading to the expression of proteins. Clonally expanded and highly differentiated CD8 T cells have been observed in individuals with TDP-43 proteinopathies and therapeutics modulating the T cell response have recently been found to extend survival.

Improvements in Wettability and Tribological Behavior of Zirconia Artificial Teeth Using Surface Micro-Textures.

Zirconia ceramics are promising materials for restoration and are widely used in the field of artificial teeth. However, wear resistance affects the longevity of artificial teeth. In this study, peacock tail feather micro-textures and groove micro-textures are prepared on the surfaces of zirconia ceramics via the laser ablation technique to improve their tribological properties.

Investigating the correlation between tertiary lymphoid structures and clinical outcomes in pancreatic ductal adenocarcinoma: insights into tumor immunology.

Pancreatic ductal adenocarcinoma (PDAC) is associated with poor prognosis and high mortality rates, necessitating the identification of reliable biomarkers for patient stratification. This study investigates the prognostic significance and biological functions of tertiary lymphoid structures (TLS) in PDAC. We analyzed clinical data from 65 PDAC patients and RNA sequencing data from The Cancer Genome Atlas (TCGA) to evaluate the presence of TLS and its impact on overall survival (OS) and recurrence-free survival (RFS).

Schistosoma japonicum cathepsin L1: A potential target for anti-schistosomiasis strategies.

Background: To achieve sustainable and integrated control of schistosomiasis, it necessitates the implementation of comprehensive strategies, where effective vaccines could play a pivotal role. The limited identification and validation of schistosome antigens hinders the progress of vaccine development for the disease. Schistosome cysteine proteinases are considered as important targets for novel anti-schistosomiasis immunoprophylaxis due to their primary role in nutrient absorption.

A sialic acid-binding protein in Toxoplasma gondii contains a conserved globular domain in apicomplexan parasites.

Background: Apicomplexan protozoans employ an intricate invasion mechanism involving dynamic interactions with host cells, characterized by sequential secretion of adhesins and lectins. Our laboratory previously identified TgSABP1, a novel Toxoplasma gondii adhesin, demonstrating specific binding affinity for sialic acid (SA) receptors on host cell surfaces. However, the structural determinants governing SA recognition by this adhesin remain undefined.

LINC00525 drives aggressive phenotypes in bladder cancer via YAP stabilization-mediated transcriptional activation.

LINC00525, a long noncoding RNA (lncRNA), has been implicated in the regulation of cancer progression across various types. However, its role in bladder cancer (BLCA) remains unconfirmed. In this study, we observed upregulation of LINC00525 expression in both bladder cancer tissues and cell lines compared to normal controls.

TIGIT blockade improves anti-Mycobacterium tuberculosis immunity.

Despite the therapeutic benefit of immune checkpoint blockade in cancers, there is no consensus on its effect in infectious diseases. Here we investigated whether blocking the immune checkpoint T cell immunoreceptor with immunoglobulin and immunoreceptor tyrosine-based inhibitory motif domains (TIGIT) increases T cell immunity in active Mycobacterium tuberculosis infection. TIGIT expression in both peripheral blood and lung lesions in tuberculosis patients was assessed, and the correlation with clinical features analyzed.

Anxiolytic Effects of L. Oligo-Polysaccharides by Modulating Gut Microbiota, Neuronal Signaling Pathways, and Neuroinflammation in Chronic Sleep Deprivation-Stressed Mice.

Prolonged sleep deprivation impairs brain function and increases the risk of mental health disorders. L. Oligo-polysaccharides (JSO), bioactive compounds derived from chicory, belong to the category of food-medicine homologous substances, possess gut microbiota-modulating and anti-inflammatory properties, and serve as a natural prebiotic, having significant research value in food science.