Prognostic Value of Late Gadolinium Enhancement Cardiac MRI-derived Entropy in Patients after Myocardial Infarction.

Purpose To investigate the prognostic value of left ventricular (LV) and scar entropy for major adverse cardiac events (MACEs) and ventricular arrhythmias (VAs) in patients who have experienced myocardial infarction (MI). Materials and Methods The medical records of patients who underwent late gadolinium enhancement (LGE) cardiac MRI following MI between October 2015 and September 2022 were retrospectively evaluated for MACEs and VAs. LV and scar entropy data were derived from the probability distributions of the pixel signal intensities of the LV myocardium and scar tissue, respectively.

PCSK9 Loss-of-Function Disrupts Cellular Microfilament Network via LIN28A/HES5/JMY Axis in Neural Tube Defects.

Neural tube defects (NTDs) are complex multigenic disorders and are the most prevalent and severe congenital malformations that affect the central nervous system. PCSK9 is identified as a molecular marker for the prenatal diagnosis of NTDs during its early stages in fetuses; however, its role in NTD neurulation and pathogenesis remains unclear. This study introduces PCSK9 knockout embryonic stem cells (ESCs) into neural organoid (NO) and neural progenitor cell (NPC) models and finds that PCSK9 loss leads to an incomplete neural tube structure in NOs and microfilament network disorder in NPCs.

Single-Entity Resolution Single-Cell Nanosensor Reveals Reactive Oxygen Species at Stress Granules Are Formed by Interfacial Redox Chemistry.

The electrochemical activities of biomolecular condensates represent a new fundamental functioning mechanism in biochemistry and cell biology. However, our understanding of the underlying molecular mechanism and the interfacial field-dependent chemical activities remains limited. This is due to the lack of technology to probe such activities in real time and at a single-condensate level.

Thirty-Year Trends in Anxiety Disorders Incidence Across China, Japan, and Republic of Korea: An Age-Period-Cohort Analysis Based on GBD 2021.

East Asia accounts for the highest number of anxiety disorders cases globally, with China, Japan, and Republic of Korea representing 17.5% of global incidence according to GBD 2021. These nations offer a unique context for examining how different modernization paths impact anxiety disorders patterns.

Elucidating the Binding Mechanism of Oclacitinib With Bovine Serum Albumin: A Multidisciplinary Approach Combining Spectroscopy, Thermodynamics, and Molecular Docking.

This paper elucidates the in vitro binding of oclacitinib (OLTN) with BSA through a multifaceted approach that combines experimental methodologies and theoretical computations. Spectroscopic analyses reveal that OLTN significantly attenuates the intrinsical fluorescence of BSA through the formation of an OLTN-BSA complex, indicating a static quenching mechanism. Experimental data further confirmed the formation of a nonfluorescent OLTN-BSA complex.

Imaging Features and Clinical Characteristics of Granular Cell Tumors: A Single-Center Investigation.

Granular cell tumors (GCTs) are rare neurogenic tumors with Schwann cell differentiation. Although most are benign, 1-2% exhibit malignant behavior. The imaging features of GCTs remain poorly characterized due to their rarity and anatomic variability.

Utilization of High Iron Content Sludge and Ash as Partial Substitutes for Portland Cement.

Sludge is a semi-solid waste generated during the process of wastewater treatment. Due to the addition of polymerized ferric chloride in the flocculation process, the sludge produced by the sewage treatment plant in Liaocheng Jiaming Industrial Park contains a high content of iron oxide. In this paper, chemical analysis and particle size analysis of local sludge and sludge ash were conducted.

A novel tsRNA signature -tRF-58:76-Tyr-GTA-2-M3 as potential biomarker and therapeutic target for duodenal atresia.

Duodenal atresia (DA) is a common neonatal digestive tract obstruction, with unclear prenatal diagnostic specificity and optimal intervention timing. tRNA-derived small RNAs (tsRNAs), stable and enriched in blood, are promising biomarkers for disease diagnosis. Therefore, identifying tsRNA biomarkers, elucidating DA pathogenesis, and exploring potential intrauterine interventions is urgently needed.

Nerandomilast in Patients with Progressive Pulmonary Fibrosis.

Background: Nerandomilast (BI 1015550) is an orally administered preferential inhibitor of phosphodiesterase 4B with antifibrotic and immunomodulatory properties. Nerandomilast has been shown to slow the progression of idiopathic pulmonary fibrosis, but an assessment of its effects in other types of progressive pulmonary fibrosis is needed.

Methods: In a phase 3, double-blind trial, we randomly assigned patients with progressive pulmonary fibrosis in a 1:1:1 ratio to receive nerandomilast at a dose of 18 mg twice daily, nerandomilast at a dose of 9 mg twice daily, or placebo, with stratification according to background therapy (nintedanib vs.

Combining lipidomics and machine learning to identify lipid biomarkers for nonsyndromic cleft lip with palate.

Nonsyndromic cleft lip with palate (nsCLP) is a common birth defect disease. Current diagnostic methods comprise fetal ultrasound images, which are mainly limited by fetal position and technician skills. We aimed to identify reliable maternal serum lipid biomarkers to diagnose nsCLP.

Methane Valorization to Antioxidant Polysaccharides by Methanotrophic Bacteria.

Bioconversion of methane into functional polysaccharides presents a promising strategy for mitigating methane-induced greenhouse gas emissions and addressing the limitations of plant-derived polysaccharide and sugar-based microbial polysaccharides production. In this study, the novel methane-derived polysaccharide (MePS) was obtained from a newly isolated methanotrophic bacterium WONF2802. Structural characterization found that MePS is a branched-chain glucan with a weight-average molecular weight of 283.

Epicardial Adipose Tissue and Extracellular Volume Fraction in Patients with Hypertrophic Cardiomyopathy: A Multi‑center Prognosis Study.

Rationale And Objectives: Epicardial adipose tissue (EAT) is thought to have a deleterious effect on the progression of myocardial disease; the extracellular volume (ECV) fraction has been validated by histology to correlate with adverse myocardial remodeling. The aim of this study was to investigate the prognostic value of EAT volume index (EATVI) and ECV in patients with hypertrophic cardiomyopathy (HCM).

Materials And Methods: ECV and EAT were measured using cardiac magnetic resonance (CMR) imaging in 201 subjects with HCM.

Insulin resistance aggravates myocardial fibrosis in non-diabetic hypertensive patients by altering the function of epicardial adipose tissue: a cardiac magnetic resonance study.

Background: The effect of insulin resistance (IR) on epicardial adipose tissue (EAT) remains uncertain. This study aimed to investigate how early-stage IR influences EAT, contributing to myocardial fibrosis and left ventricular dysfunction in non-diabetic patients with hypertension.

Methods: A total of 166 hypertensive patients who underwent cardiovascular magnetic resonance (CMR) treatment at two medical centers in China from June 2015 to August 2024 were included.

Risk-adapted scoring model to identify candidates benefiting from adjuvant chemotherapy after radical nephroureterectomy for localized upper urinary tract urothelial carcinoma: A multicenter study.

Purpose: Adjuvant chemotherapy (AC) is recommended for muscle-invasive or lymph node-positive upper urinary tract urothelial carcinoma (UTUC) after radical nephroureterectomy (RNU). However, disease recurrences are frequently observed in pT1 disease, and AC may increase the risk of overtreatment in pT2 UTUC patients. This study aimed to validate a risk-adapted scoring model for selecting UTUC patients with ≤pT2 disease who would benefit from AC.

Sympathetic neurons can modify the intrinsic structural and functional properties of human pluripotent stem cell-derived cardiomyocytes.

The sympathetic nervous system densely innervates all cardiac chambers and is a key player in cardiac control, yet this relationship has scarcely been investigated using a stem cell-based model. This study investigates the effects that sympathetic neurons (SNs) have on human pluripotent stem cell-derived cardiomyocytes (hPSC-CMs) in vitro, and whether they induce any degree of functional or structural maturity in these conventionally immature cells. SNs were isolated from neonatal rat pups, and cocultured with hPSC-CMs for up to 15 days.

Investigations into Replacing Calcined Clay with Sewage Sludge Ash in Limestone Calcined Clay Cement (LC3).

The effects of replacing calcined clay with sewage sludge ash (SSA) treated under room temperature and high temperature ranging from 500 °C to 900 °C in limestone calcined clay cement (LC3) have been investigated in this paper. The optimal calcination temperature for SSA was found to be 800 °C based on the results of strength and microstructure observations. The main inorganic components of sludge ash are FeO, SiO, AlO, and CaO, which are very similar to the components of calcined clay in LC3, but with a very high content of FeO (55-61%) and PO (9-10%).

Thermoresponsive gel polymer electrolytes for smart rechargeable zinc-ion batteries.

Thermoresponsive double-network hydrogels based on poly(-isopropylacrylamide) (PNIPAM) with lower critical solution temperature (LCST) were developed to prevent the thermal runaway of rechargeable zinc-ion batteries (ZIBs) due to rapid, reversible, and intelligent blocking of the zinc ion transport channels at a specific trigger temperature.

Phase II Randomized Trial of BI 730357, an Oral RORγt Inhibitor, for Moderate-to-Severe Plaque Psoriasis.

Trial Design: This 2-part, double-blinded trial assessed the RORγt inhibitor BI 730357 in plaque psoriasis.

Methods: In part 1, patients were randomized 2:2:2:2:1 to 25, 50, 100, and 200 mg BI 730357 or placebo once daily (fasting conditions); nonresponders were switched to higher doses. In part 2, a separate patient set was randomized 4:4:1 to receive BI 730357 (400 mg once daily, 200 mg twice daily) or a placebo (fed conditions).

ApoM maintains cellular homeostasis between mitophagy and apoptosis by affecting the stability of Nnt mRNA through the Zic3-ApoM-Elavl2-Nnt axis during neural tube closure.

Research on the aetiology of neural tube defects (NTDs) has made progress in recent years. However, the molecular mechanism of apolipoproteins underlying NTDs development remains unclear. This study aimed to investigate the function of apolipoprotein M (ApoM) in the pathogenesis of NTDs and its underlying mechanisms.

Prognostic Value of Epicardial Adipose Tissue in Heart Failure With Mid-Range and Preserved Ejection Fraction: A Multicenter Study.

Background: Epicardial adipose tissue (EAT) accumulation is thought to play a role in the pathophysiology of heart failure (HF) with mid-range ejection fraction and HF with preserved ejection fraction, but its effect on outcome is unknown.

Methods And Results: A total of 692 patients with HF with mid-range ejection fraction or HF with preserved ejection fraction who underwent cardiovascular magnetic resonance at 2 medical centers in China between October 2016 and October 2022 were included in this study. EAT volume and extracellular volume were calculated using cardiovascular magnetic resonance.

Polysaccharides isolated from Ampelopsis grossedentata and their immunomodulatory activity.

To explore the immunomodulatory activity of polysaccharides from Ampelopsis grossedentata, two polysaccharides named AGP1 and AGP2 were isolated and purified by DEAE-cellulose 52 column and Sephacryl S-300HR chromatography. AGP1 and AGP2 were composed of fucose, arabinose, rhamnose, galactose, glucose, mannose, galacturonic acid, and glucuronic acid, with a ratio of 0.5: 10.

Predictive value of pericoronary fat attenuation index for graft occlusion after coronary artery bypass grafting.

Purpose: Based on coronary computed tomography angiography (CCTA), this study aimed to evaluate the predictive value of pericoronary fat attenuation index (FAI) for graft occlusion in patients following coronary artery bypass grafting (CABG).

Materials And Methods: The clinical and imaging data of 100 patients with coronary artery disease (CAD) who underwent CCTA and subsequently received successful CABG between December 2012 and March 2024 were retrospectively collected. According to the subsequent CCTA evaluation of grafts, they were categorized into occlusion group (n = 27) and patency group (n = 73).

Prognostic value of extracellular volume fraction in myocardial infarction and myocardial infarction with nonobstructive coronary arteries: A multicenter study.

Rationale And Objectives: The aim of the present retrospective study was to evaluate the prognostic role of the extracellular volume fraction (ECV) in patients with myocardial infarction (MI) and myocardial infarction with nonobstructive coronary arteries (MINOCA). The present study hypothesized that ECV is associated with major adverse cardiovascular events (MACEs) in MI and MINOCA patients.

Materials And Methods: Cardiac magnetic resonance (CMR) imaging was performed on 351 consecutive patients (mean age: 58 ± 12 years; 252 [71.

Development of a Photoelectrochemical Microelectrode Using an Organic Probe for Monitoring Hydrogen Sulfide in Living Brains.

Hydrogen sulfide (HS) is an important bioactive molecule that plays a significant role in various functions, particularly in the living brain, where it is closely linked to cognition, memory, and several neurological diseases. Consequently, developing effective detection methods for HS is essential for studying brain functions and the underlying mechanisms of these diseases. This study aims to construct a novel photoelectrochemical (PEC) microelectrode Ti/TiO@HSP for the quantitative monitoring of HS levels in the living brain.

The Interrelation of Blood Urea Nitrogen-to-Albumin Ratio with Three-Month Clinical Outcomes in Acute Ischemic Stroke Cases: A Secondary Analytical Exploration Derived from a Prospective Cohort Study.

Objective: This study targeted elucidating the intricate correlation of the blood urea nitrogen (BUN)-to-albumin (BUN/Alb) ratio with adverse outcomes (AOs) at 3-month in acute ischemic stroke (AIS) cases within a Korean cohort.

Methods: The cohort involved a comprehensive dataset of 1850 AIS cases from a South Korean hospital, spanning from January 2010 to December 2016. To discern the linear relationship of the BUN/Alb ratio with AOs in AIS cases, utilization of a binary logistic regression model (BLRM) was implemented.