Volatile Organic Compounds in Teas: Identification, Extraction, Analysis, and Application of Tea Aroma.

Volatile organic compounds (VOCs) are important for teas' quality and act as a critical evaluative criterion in teas. The distinctive aromatic profile of tea not only facilitates tea classification but also has potential applications in aroma-driven product innovation. In this review, we summarized the tea aroma from tea classification, VOCs extraction methodologies, and VOCs detection techniques.

A proximity cleavage assisted DNAzyme catalyzed loading DNA walker system for accurate and sensitive small extracellular vesicle analysis.

The significant biological functions and clinical importance of small extracellular vesicles (sEVs) in lung malignancies make them crucial diagnostic markers. However, the current detection methods for cancer related sEVs typically recognize single molecules on the surface of sEVs, which lack enough specificity. We developed a proximity cleavage-assisted DNAzyme-catalyzed loading DNA walker system for the precise investigation of sEVs.

Spatiotemporal evolution, driving factors, and policy impacts on ecological quality in the li river basin from 1995 to 2021.

Ecological quality (EQ) protection constitutes a cornerstone of sustainable development. This study develops a Remote Sensing Ecological Index (RSEI) via the Google Earth Engine (GEE) platform, integrating Theil-Sen slope estimation, spatial autocorrelation, GeoDetector, geographically weighted regression (GWR), and text mining to investigate EQ dynamics in the Li River Basin (1995-2021). Key findings reveal: (1) The basin exhibited a "low-central, high-peripheral" RSEI pattern with a fluctuating upward EQ trend that peaked in 2003 (forest conservation and urban green policies) and 2009 (accelerated farmland-to-forest conversion and energy-saving initiatives).

Quantification of Tryptophan Enantiomers in a Single Cell by β-Cyclodextrin-Modified Carbon Nanopipettes.

Tryptophan levels in the human body are closely related to disease development and metabolic processes, but identification and quantification of tryptophan enantiomers at the single-cell level is still very challenging now. Herein, the mono-(6-ethanediamine-6-deoxy)-β-cyclodextrin (β-CD)-modified carbon nanopipet (CNP) was fabricated, and high-enantioselective electrochemical detection of tryptophan was achieved. Interestingly, the selectivity of the prepared CNP toward l-tryptophan (l-Trp) and d-Tryptophan (d-Trp) could be modulated by adjusting the solution pH.

Integrated Physiological, Transcriptomic, and Metabolomic Analysis Reveals Mechanism Underlying the -Enhanced Drought Tolerance in Tea Plants.

Drought stress significantly impairs the output of tea plants and the quality of tea products. Although has demonstrated the ability to enhance drought tolerance in host plants, its impact on tea plants () experiencing drought stress is unknown. This study assessed the response of tea plants by inoculating under drought conditions.

Investigating the modulatory effects of Pu-erh tea on the gut microbiota in ameliorating hyperuricemia induced by circadian rhythm disruption.

Circadian rhythm disruption (CRD) can induce a variety of metabolic disorders. Our previous laboratory studies have shown that Pu-erh tea could alleviate CRD-induced syndromes, including obesity, intestinal dysfunction, and tryptophan metabolism disorders. However, its potential protective mechanism against CRD-induced hyperuricaemia remains unclear.

Serum lipid profiling reveals characteristic lipid signatures associated with stroke in patients with leukoaraiosis.

Many lipid biomarkers of stroke have been identified, but the lipid metabolism in elderly patients with leukoaraiosis remains poorly understood. This study aims to explore lipid metabolic processes in stroke among leukoaraiosis patients, which could provide valuable insights for guiding future antithrombotic therapy. In a cohort of 215 individuals undergoing MRI, 13 stroke patients were matched with controls, and 48 stroke patients with leukoaraiosis were matched with 40 leukoaraiosis patients.

Comparing the utility of lung function parameters and fractional exhaled nitric oxide in predicting lung cancer.

This study aimed to investigate the potential clinical factors that may be associated with the incidence of lung cancer. A total of 150 individuals were enrolled in this cohort study, of which 78 were diagnosed with lung cancer. The results of this study revealed some interesting findings.

A broadband modeling method for range-independent underwater acoustic channels using physics-informed neural networks.

Accurate broadband modeling of underwater acoustic channels is vital for underwater acoustic detection, localization, and communication. Conventional modeling methodologies, based on methods such as the finite element method, finite difference method, and boundary element method, generally facilitate computation for only a single frequency at a time. However, in broadband modeling, this characteristic presents limitations, requiring multiple computations across frequencies, thereby leading to significant time challenges.

Well-differentiated inflammatory liposarcoma in the muscularis of the gallbladder.

Inflammatory liposarcoma is one of the rarest subtypes of well-differentiated liposarcoma. We present an extremely rare case of well-differentiated inflammatory liposarcoma that occurs in the muscularis of the gallbladder, which was difficult to diagnose before surgery due to the lack of specific clinical and imaging findings. Since cyclin-dependent kinase 4 (CDK4) and murine double minute 2 (MDM2) both displayed amplification in this case, they are not only important markers for auxiliary diagnosis but also the focus of current targeted therapy.

Fast and accurate wideband sparse spatial spectrum estimation in an underwater strong interference environment.

Wideband sparse spatial spectrum estimation is an important direction-of-arrival (DOA) estimation method that can obtain a high resolution with few snapshots and a low signal-to-noise ratio. However, in an underwater strong interference environment, the accuracy of DOA estimation may be seriously affected, and even the weak targets could be completely masked. In this paper, we propose a fast matrix filter design method based on truncated nuclear norm regularization to attenuate strong interferences while passing weak targets.

Sensitive and Extraction-Free Detection of Methicillin-Resistant through Ag Aptamer-Based Color Reaction.

Refractory infections, such as hospital-acquired pneumonia, can be better diagnosed with the assistance of precise methicillin-resistant (MRSA) testing. However, traditional methods necessitate high-tech tools, rigorous temperature cycling, and the extraction of genetic material from MRSA cells. Herein, we propose a sensitive, specific, and extraction-free strategy for MRSA detection by integrating allosteric probe-based target recognition and exonuclease-III (Exo-III)-enhanced color reaction.

Multi-omics reveal neuroprotection of Acer truncatum Bunge Seed extract on hypoxic-ischemia encephalopathy rats under high-altitude.

Hypoxic-ischemic encephalopathy (HIE) at high-altitudes leads to neonatal mortality and long-term neurological complications without effective treatment. Acer truncatum Bunge Seed extract (ASO) is reported to have effect on cognitive improvement, but its molecular mechanisms on HIE are unclear. In this study, ASO administration contributed to reduced neuronal cell edema and improved motor ability in HIE rats at a simulated 4500-meter altitude.

Multilayer omics landscape analyses reveal the regulatory responses of tea plants to drought stress.

Adverse environments, especially drought conditions, deeply influence plant development and growth in all aspects, and the yield and quality of tea plants are largely dependent on favorable growth conditions. Although tea plant responses to drought stress (DS) have been studied, a comprehensive multilayer epigenetic, transcriptomic, and proteomic investigation of how tea responds to DS is lacking. In this study, we generated DNA methylome, transcriptome, proteome, and phosphoproteome data to explore multiple regulatory landscapes in the tea plant response to DS.

Towards an Optimized Distributed Message Queue System for AIoT Edge Computing: A Reinforcement Learning Approach.

The convergence of artificial intelligence and the Internet of Things (IoT) has made remarkable strides in the realm of industry. In the context of AIoT edge computing, where IoT devices collect data from diverse sources and send them for real-time processing at edge servers, existing message queue systems face challenges in adapting to changing system conditions, such as fluctuations in the number of devices, message size, and frequency. This necessitates the development of an approach that can effectively decouple message processing and handle workload variations in the AIoT computing environment.

Lipidomics reveal the cognitive improvement effects of seed oil on hypoxic-ischemic encephalopathy rats.

Hypoxic-ischemic encephalopathy (HIE) is one of the leading causes of acute neonatal death and chronic neurological damage, and severe HIE can have secondary sequelae such as cognitive impairment and cerebral palsy, for which effective interventions are lacking. In this study, we found that continuous 30-day intake of seed oil (ASO) reduced brain damage and improved cognitive ability in HIE rats. Using lipidomic strategies, we observed that HIE rats had decreased unsaturated fatty acids and increased lysophospholipids in the brain.

Transcriptome Analysis Reveals That Ascorbic Acid Treatment Enhances the Cold Tolerance of Tea Plants through Cell Wall Remodeling.

Cold stress is a major environmental factor that adversely affects the growth and productivity of tea plants. Upon cold stress, tea plants accumulate multiple metabolites, including ascorbic acid. However, the role of ascorbic acid in the cold stress response of tea plants is not well understood.

Aroma characteristics of Wuyi rock tea prepared from 16 different tea plant varieties.

Wuyi rock tea (WRT) is famous for its long history and unique characteristic of floral, fruity and nutty flavors. This study investigated the aroma characteristics of WRTs prepared from 16 different oolong tea plant varieties. The sensory evaluation results showed that all WRTs had an 'Yan flavor' taste, and the odor was strong and lasting.

Experimental Determination of an Isolated trans-Dinitrosyl Manganese(II) Heme Analogue.

The lack of direct proof in either natural or synthetic systems for trans-dinitrosyl hemes, a key intermediate in the reactions of heme proteins (e.g. soluble guanylate cyclase (sGC), cytochrome c' and So H-NOX) with nitric oxide (NO), has hampered understanding of the exact reaction mechanisms, such as the formation of the five-coordinate heme complex with NO at the proximal side (5c NO ).

Nitrato(5,10,15,20-tetra-phenyl-porphinato)manganese(III)-benzene--hexa-ne (2/1/1).

The crystal structure of solvated [Mn(TPP)(NO)] (TPP = 5,10,15,20-tetra-phenyl-porphyrinato, CHN), [Mn(CHNO)(NO)]·0.5CH·0.5CH, has been determined in the space group .

DNA Compatible Oxidization and Amidation of Terminal Alkynes.

Through a modified Kinugasa reaction, a novel method of amidation on terminal oligo alkyne conjugates by copper-promoted oxidation with nitrones has been developed. Unprotected bifunctional carboxylic acid-amine reagents can be transformed directly to the respective amide products under these edited Kinugasa reaction conditions. 3-Cycle DNA-encoded libraries (DELs) can be built in three steps of chemical conversion.

Extracellular Vesicles-Encapsulated miR-153-3p Potentiate the Survival and Invasion of Lung Adenocarcinoma.

Extracellular vesicles (EVs) play an essential role in the communication between cells and the tumor micro-environment. However, the effect of tumor-derived EVs on the growth and metastasis of lung adenocarcinoma (LUAD) remains to be explored. This study aimed to elucidate the role of miR-153-3p-EVs in the invasion and migration capabilities of LUAD cells and explore its mechanism through and experiments.

The quantitative evaluation of contrast-enhanced ultrasound in the differentiation of small renal cell carcinoma subtypes and angiomyolipoma.

Background: Contrast-enhanced ultrasound (CEUS) has been widely used for renal lesion diagnosis and differential diagnosis. However, qualitative analysis of CEUS is subject to examinations with low reproducibility. This study aims to investigate the diagnostic value of CEUS quantitative parameters in differentiating small renal cell carcinoma (RCC) subtypes and angiomyolipoma (AML).