The immunological and prognostic landscape of TFAP4 in cancer: A single-cell RNA sequencing perspective.

Transcription factor activating enhancer-binding protein 4 (TFAP4) has been implicated in tumorigenesis, yet its precise role in the tumor microenvironment remains inadequately characterized. In this study, we employed single-cell RNA sequencing and multi-omics analysis to investigate the immunological and prognostic significance of TFAP4 across various cancer types. Our analysis reveals that TFAP4 is upregulated in malignant epithelial cells across multiple cancer types and correlated with advanced tumor stages and poor patient survival.

Esculin delivery system based on phospholipid complex technology: research on solubility optimization and antibacterial activity improvement.

Esculin (ES) has antibacterial effects, but its low solubility and bioavailability limit applications. This study prepared an esculin phospholipid complex (ES-PC) solvent evaporation. SEM, FTIR, XRD and H-NMR verified its amorphous structure formed by intermolecular forces.

Loading esculin can improve the morphology and antibacterial properties of chitosan/whey protein isolate composite film.

In this study, chitosan (CTS), whey protein isolate (WPI) and glycerol were used as biodegradable film-forming matrices to prepare composite films by solution blending. Esculin (ES) as antibacterial active compound, was added during the film modification process. CTS, CTS/WPI (C/W) and CTS/WPI/ES (C/W/ES) films were compared in terms of structure, physical properties and functions.

Preparation, characterisation and evaluation of kaempferol phospholipid complex and its application in preventing the deterioration of soybean oil.

This study aims to improve the solubility of kaempferol (KA) by preparing a kaempferol phospholipid complex (KA-PC), which can be applied to prevent soybean oil from spoilage. KA-PC was prepared by solvent evaporation and characterised by FTIR, DSC, and XRD. Furthermore, KA-PC was added to soybean oil and the peroxide value and acid value were determined.

Preparation and characterisation of kaempferol composite carrier solid dispersion: evaluation of its application in preventing soybean oil spoilage.

Kaempferol (KPF) can be used as a natural antioxidant and food additive in food processing. However, the poor solubility of KPF limited its bioavailability and application. In order to improve the solubility of KPF, kaempferol composite carrier solid dispersion (KPF-CC-SD) was prepared and the process was optimised.

Melittin alleviates sepsis-induced acute kidney injury by promoting GPX4 expression to inhibit ferroptosis.

Objectives: Melittin, the main component of bee venom, is a natural anti-inflammatory substance, in addition to its ability to fight cancer, antiviral, and useful in diabetes treatment. This study seeks to determine whether melittin can protect renal tissue from sepsis-induced damage by preventing ferroptosis and explore the protective mechanism.

Methods: In this study, we investigated the specific protective mechanism of melittin against sepsis-induced renal injury by screening renal injury indicators and ferroptosis -related molecules and markers in animal and cellular models of sepsis.

Analysis of spatiotemporal variations and influencing factors of soil erosion in the Jiangnan Hills red soil zone, China.

Soil erosion is an important environmental problem in China. The hilly region of Jiangnan is characterized by severe soil erosion due to its unique climate and intensive human activities. Therefore, assessing soil erosion in this area is of great significance for achieving regional sustainable development.

PEO/cellulose composite paper based triboelectric nanogenerator and its application in human-health detection.

Recently, cellulose paper based triboelectric nanogenerators (CPTENGs) has gained widely attention due to the development of wearable, green and miniaturized electronic products. Modification of cellulose fibers or paper is a feasible method to improve the output performance of CPTENGs, however, the simple and effective routes to improve the triboelectric property of cellulose paper still remain a challenge. Herein, we report a simple method to prepare PEO/cellulose composite paper (PEO/CCP) via mixing polyethylene oxide (PEO) with cationic cellulose fibers.

Bioactivity and Component Analysis of Water Extract of against Hyperuricemia by Inhibiting Xanthine Oxidase Activity.

Hyperuricemia (HUA) is a metabolic condition caused by excessive production or low excretion of uric acid (UA) in the body. Xanthine oxidase (XOD) is the key enzyme in the process of metabolism purines to generate UA. In this study, the in vitro inhibitory effect of water extract of the flower bud of (WESJ) on XOD was investigated by ultraviolet spectrophotometry.

Bifidobacterium breve predicts the efficacy of anti-PD-1 immunotherapy combined with chemotherapy in Chinese NSCLC patients.

Background And Purpose: Gut microbes play an important role in the occurrence of lung cancer, immunotherapy, and chemotherapy. In this study, we analyzed the characteristics of gut microbes in patients with lung cancer and investigated the effect of gut microbes on anti-PD-1 therapy combined with chemotherapy.

Methods: Fecal samples from 21 non-small cell lung cancer (NSCLC) patients and 22 healthy volunteers who were treated in the Fourth Hospital of Hebei Medical University from 2019 to 2021 were collected.

Preparation of an isorhamnetin phospholipid complex for improving solubility and anti-hyperuricemia activity.

To improve the solubility and anti-hyperuricemia activity of the insoluble natural flavonoid isorhamnetin (ISO), an isorhamnetin phospholipid complex (ISO-PC) was prepared. ISO-PC was prepared through solvent evaporation and its prescription process was optimized. The formation of ISO-PC was verified via multiple characterization methods.

Extracellular vesicle-packaged miR-181c-5p from epithelial ovarian cancer cells promotes M2 polarization of tumor-associated macrophages via the KAT2B/HOXA10 axis.

Objectives: The molecular mechanistic actions of tumor-derived extracellular vesicles (EVs) in modulating macrophage polarization in the tumor microenvironment of epithelial ovarian cancer (EOC) is largely unknown. The study was performed to clarify the effect and downstream mechanism of microRNA-181c-5p (miR-181c-5p)-containing EVs from EOC cells in the M2 polarization of tumor-associated macrophages (TAMs).

Methods: EVs were isolated from normoxic and hypoxic human EOC cells SKOV3.

Opportunities and challenges in microwave absorption of nickel-carbon composites.

In recent years, the problem of electromagnetic wave (EMW) pollution has attracted more and more attention with the development of science and technology. In order to solve this complex problem, the research and development of EMW-absorbing materials is crucial. The new absorbing materials should have the characteristics of light weight, high efficiency, wide bandwidth, environmental protection, oxidation resistance, and other characteristics.

Quantitation of vistusertib by UHPLC-MS/MS in rat plasma and its application to a pharmacokinetic study.

The present study aimed to develop a UHPLC-MS/MS method for determination of vistusertib in biological matrix, and to describe the pharmacokinetic behavior of vistusertib in SD rats. After protein precipitation with acetone and acetonitrile (1:1), the chromatographic separation was achieved on an Agilent Poroshell 120 EC-C column and detected with a SCIEX QTRAP 4500 mass spectrometer under positive ionization mode. The developed UHPLC-MS/MS method showed an excellent linearity within the range of 1.

Signal extraction using complementary ensemble empirical mode in pipeline magnetic flux leakage nondestructive evaluation.

The magnetic flux leakage (MFL) evaluation is often used for the overhauling of oil extracting operation in the oil field to realize the real-time damage assessment of the pipeline. Since the MFL signal is affected by various noise sources in the field, this paper introduces the complementary ensemble empirical mode decomposition with adaptive noise (CEEMDAN). On the basis of this, a particle swarm optimization wavelet threshold (PSO-WT) method is proposed, and the signal reconstruction option is improved to extract the leakage magnetic flux signal of tubing defects.

Expression of MIF and c-erbB-2 in endometrial cancer.

The aim of the present study was to investigate the expression of c-erbB-2 and macrophage migration inhibitory factor (MIF) in endometrial cancer and to elucidate the significance of the early diagnosis and prognosis of endometrial cancer. The gene copy number of c‑erbB‑2 and MIF was characterized by reverse transcription quantitative polymerase chain reaction and the reactivity was assessed by immunohistochemistry in 70 patients using a polyclonal antibody, and evaluated semiquantitatively according to the percentage of cells demonstrating membranous or diffuse cytoplasmic staining. A correlation between age, tumor stage, grade, myometrial invasion and lymph node metastasis was observed.

Impacts of laparoscopic hysterectomy on functions of coagulation and fibrinolysis system.

The main objective of the study is to compare the impacts of laparoscopic hysterectomy and total abdominal hysterectomy on the functions of coagulation and fibrinolysis system. Seventy-five patients who had undergone hysterectomy were randomly divided into laparoscopic hysterectomy group (n = 38) and total abdominal hysterectomy group (n = 37). The prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen, D-dimer, von Willebrand factor, α-granule membrane protein-140, thrombin-activated fibrinolysis inhibitor (TAFI) and platelet count were detected at preoperative 24 h (N0), postoperative 24 h (N1) and postoperative 48 h (N2).

Associations between antioxidant vitamins and the risk of invasive cervical cancer in Chinese women: A case-control study.

Previous studies on the associations between dietary antioxidant vitamins and the risk of cervical cancer remain inconsistent, and little evidence is available for serum antioxidant vitamins, which provide more accurate measurements of these nutrients. We conducted a case-control study of 458 incident cases with invasive cervical cancer and 742 controls to assess the effects of diet or serum antioxidant vitamins. Higher serum antioxidant vitamins were associated with a lower risk of cervical cancer after adjusting for potential confounders.

RNAi-mediated knockdown of the CLN3 gene inhibits proliferation and promotes apoptosis in drug-resistant ovarian cancer cells.

CLN3 is a recently identified anti-apoptotic gene, which has been demonstrated to be highly expressed in a diverse range of cancer cell lines, including ovarian cancer. In the present study, RNA interference, mediated by a lentivirus expressing CLN3 short hairpin RNA (shRNA) was utilized to knockdown the expression of CLN3 in the A2780 human ovarian cancer cell line, and its cisplatin‑resistant and carboplatin‑resistant sublines, A2780/DDP and A2780/CBP cells. It was revealed that the mRNA and protein expression levels of CLN3 were significantly reduced in the CLN3‑specific shRNA‑transduced cells, compared with the untransduced and control shRNA‑transduced cells.