Activation of PP2A-B56α leads to aberrant EGFR signaling and proliferative phenotypes in PDAC.

Pancreatic ductal adenocarcinoma (PDAC) stands to become the second most deadly cancer by 2030. The small GTPase, KRAS, is mutated in over 90% of PDAC patients and considered the primary driver mutation. Despite being an almost ubiquitous event, KRAS mutations have been difficult to target therapeutically, particularly KRAS , the most common mutation in PDAC.

In vivo modeling of lethal congenital contracture syndrome 1 suggests pathomechanisms in cellular stress responses.

The mRNA export factor GLE1 protein plays critical yet enigmatic functions in RNA processing and has been linked with multiple developmental disorders, including lethal congenital contracture syndrome 1 (LCCS1). Using in vivo genetic engineering to study disturbed GLE1 functions under physiological conditions, we demonstrate that total inactivation of GLE1 results in disorganization of the blastocyst inner cell mass and early embryonic lethality due to defects in lineage specification. In contrast, the knock-in mice genocopying the LCCS1-associated GLE1 variant (Gle1) survive the prenatal period but die suddenly at midadulthood.

Embedding Fe/FeO Heterojunctions in Nitrogen-Doped Carbon Nanotubes for Efficient Removal of Organic Pollutants via Heterogeneous Electro-Fenton Catalysis.

The electro-Fenton process is considered one of the most reliable techniques for eliminating organic pollutants from water. However, its performance is still not up to par with its potential due to the lack of cathodic catalysts with the essential cycling catalytic functionality required for sustained Fenton reactions. Herein, Fe and FeO heterojunctions embedded in N-doped carbon nanotubes stitched on porous nitrogen-doped carbon (denoted Fe/FeO@PNC) are fabricated and employed as a bifunctional cathode catalyst for the highly efficient degradation of organic pollutants.

VEGFA induces anxiety and depression-like behaviors in post-stroke mice by regulating the permeability of the blood-brain barrier.

Post-stroke depression (PSD) is the most common neuropsychiatric sequela of stroke, and its pathogenesis remains unclear. The blood-brain barrier (BBB) is an important barrier for maintaining the normal operation of neurons. The impairment of its function leads to the occurrence of various neurological diseases.

Identification and validation of differentially expressed genes in intramuscular fat metabolism in Guizhou yellow chickens using RNA-Seq analysis.

Intramuscular fat (IMF) content is crucial for meat quality, and genetic, environmental, and nutritional factors influence its deposition. This study aims to identify genes involved in the regulation of IMF deposition in Guizhou Yellow Chickens. Thirty Guizhou Yellow Chicken hens aged 120 days were selected, and their IMF content was measured using the Soxhlet extraction method.

HMGB1/RAGE signaling mediates the activation of microglia and participates in depressive-like behaviors and cognitive deficits in rats after ischemia-reperfusion.

Post-stroke depression (PSD) is a common psychiatric complication that occurs after stroke, especially in ischemic stroke (I/S). It has been reported that high-mobility group box-1 (HMGB1) is highly expressed in clinical PSD patients, but the exact molecular mechanism of its involvement in PSD is not completely clear, the neuroinflammation may participate in its development. Thus, we established a PSD rat model, observed behavioral and cognitive deficits, and found that the HMGB1/ receptor for advanced glycation end products (RAGE) pathway were activated in microglia.

Dose-dependent CHCHD10 dysregulation dictates motor neuron disease severity and alters creatine metabolism.

Dominant defects in CHCHD10, a mitochondrial intermembrane space protein, lead to a range of neurological and muscle disease phenotypes including amyotrophic lateral sclerosis. Many patients present with spinal muscular atrophy Jokela type (SMAJ), which is caused by heterozygous p.G66V variant.

Bone-derived factors mediate crosstalk between skeletal and extra-skeletal organs.

Bone has long been acknowledged as a fundamental structural entity that provides support and protection to the body's organs. However, emerging research indicates that bone plays a crucial role in the regulation of systemic metabolism. This is achieved through the secretion of a variety of hormones, cytokines, metal ions, extracellular vesicles, and other proteins/peptides, collectively referred to as bone-derived factors (BDFs).

Genetic parameter estimation and molecular foundation of Double-yolk eggs trait in white leghorn.

Double-yolk (DY) eggs refer to the presence of two yolks in an egg, and they are often present in fowl flocks. As abnormal eggs, DY eggs occur frequently in the early stages of egg-laying in hens, as well as in hens with early sexual maturity. In order to understand the inheritant pattern of DY eggs and explore candidate genes associated with DY eggs, we selected over 10,000 white Leghorn (WL) chickens from 4 generations and recorded the data during the egg laying period, including total egg number and the rate of DY egg number during the first 2 months and the period of 18-58 weeks of age (EN2month, EN38, EN58, DY2month, DY38, and DY58), age at first egg (AFE), first egg weight (FEW), and body weight at first egg (BWA).

Overexpression of S100B promotes depressive-like behaviors in stroke-induced rats by modulating the PI3K/AKT/NF-κB pathway.

Post-stroke depression (PSD) is a common complication following a stroke, primarily characterized by low mood, cognitive sluggishness, and sleep disturbances. Currently, the precise pathogenic mechanisms underlying PSD remain elusive. Research indicates that S100B protein levels may serve as a specific biochemical marker of organic brain injury, with significantly elevated serum S100B levels noted in patients with ischemic stroke, depression, and schizophrenia.

rTMS ameliorates CUMS-induced anxiety-depression-like behaviour and cognitive dysfunction in rats by modulating the COX-2/PGE2 signalling pathway.

Background: rTMS is a safe and effective neuromodulation method for treating depression, but the specifics of its antidepressant effects and the underlying mechanisms remain uncertain.

Methods: Male SD rats were randomly divided into four groups: control group, CUMS group, CUMS + rTMS (10 Hz) group, and CUMS + celecoxib (25 mg/kg, as a positive control) group. Depression-like behavior was assessed by weight change, SPT, and FST; anxiety by OFT and EPM; and cognitive function by the Y-maze.

Piezo1 Regulates Odontogenesis via a FAM83G-Mediated Mechanism in Dental Papilla Cells In Vitro and In Vivo.

This study explored the role of Piezo1 in the odontogenic differentiation of dental papilla cells (DPCs) and tissue, focusing on a mechanism involving family with sequence similarity 83, member G (FAM83G). Here, we found Piezo1, a mechanosensitive cation channel, was upregulated during odontogenesis in DPCs and dental papilla tissues. Knockdown of Piezo1 impaired odontogenic differentiation, while its activation by Yoda1 enhanced the process.

Identification and validation of a major QTL, QFhb-6AL, for Fusarium head blight resistance on chromosome 6AL in wheat.

A novel major QTL, QFhb-6AL, accounting for 34.6% phenotypic variation for FHB resistance, was identified in the Chinese cultivar Xunong 029, and a near-diagnostic marker was developed for marker-assisted selection. Fusarium head blight (FHB) is a destructive disease in wheat (Triticum aestivum L.

Mapping and validation of QTkw.cau-3DL, a major QTL controlling thousand-kernel weight in wheat.

A novel major QTL, QTkw.cau-3DL, for thousand-kernel weight has been identified on the wheat chromosome arm 3DL and enhances grain yield by 6.2% under field conditions.

High-Efficiency Catalytic Ozonation Degradation of Ni Complex Wastewater Using Mn-N Codoped Active Carbon Catalyst.

With the rapid development of electroless nickel (Ni) plating industry, a large amount of Ni complex wastewater is inevitably produced, which is a serious threat to the ecological environment. Herein, a novel Mn-N codoped active carbon (Mn-N@AC) catalyst with high catalytic ozonation ability was synthesized by the impregnation precipitation method and was characterized by BET, XRD, Raman, SEM, FTIR, and TPR. Meanwhile, Mn-N@AC showed excellent catalytic ozonation ability, stability, and applicability.

The chicken cecal microbiome alters bile acids and riboflavin metabolism that correlate with intramuscular fat content.

Intramuscular fat (IMF) is a key indicator of chicken meat quality and emerging studies have indicated that the gut microbiome plays a key role in animal fat deposition. However, the potential metabolic mechanism of gut microbiota affecting chicken IMF is still unclear. Fifty-one broiler chickens were collected to identify key cecal bacteria and serum metabolites related to chicken IMF and to explore possible metabolic mechanisms.

Analysis of genetic diversity and genetic structure of indigenous chicken populations in Guizhou province based on genome-wide single nucleotide polymorphism markers.

Guizhou province in China is rich in indigenous chicken breeds, playing an essential role in the genetic improvement of modern chickens. Genetic diversity has decreased in recent decades due to accelerated breeding processes and changing conservation priorities. To determine the genetic diversity and population structure of Guizhou indigenous chicken breeds, we used 55K genotyping arrays to conduct population genetic analysis on 233 individuals from 8 Guizhou indigenous breeds and 263 individuals from 9 Guizhou indigenous chicken populations.

CUMS induces depressive-like behaviors and cognition impairment by activating the ERS-NLRP3 signaling pathway in mice.

Background And Objective: Endoplasmic reticulum stress (ERS), as a primary defense mechanism against stress, is closely related to mental disorders, but its pathogenesis is still unclear. This research seeks to explore the influence of ERS-nucleotide-bound oligomerized domain-like receptor protein 3 (NLRP3) signaling on mice's depressive-like behaviors and cognitive impairment.

Design And Method: We carried out a study on 32 male C57BL/6J mice to investigate how chronic unpredictable mild stress (CUMS) can give rise to depressive-like behaviors and cognitive dysfunction, randomly dividing them into control, model, inhibitor, and agonist groups.

Integrating microbial 16S rRNA sequencing and non-targeted metabolomics to reveal sexual dimorphism of the chicken cecal microbiome and serum metabolome.

Background: The gut microbiome plays a key role in the formation of livestock and poultry traits via serum metabolites, and empirical evidence has indicated these traits are sex-linked.

Methods: We examined 106 chickens (54 male chickens and 52 female chickens) and analyzed cecal content samples and serum samples by 16S rRNA gene sequencing and non-targeted metabolomics, respectively.

Results: The cecal microbiome of female chickens was more stable and more complex than that of the male chickens.

CRHR1 antagonist alleviated depression-like behavior by downregulating p62 in a rat model of post-stroke depression.

Post-stroke depression (PSD) is a complication of cerebrovascular disease, which can increase mortality after stroke. CRH is one of the main signaling peptides released after activation of the hypothalamic-pituitary-adrenal (HPA) axis in response to stress. It affects synaptic plasticity by regulating inflammation, oxidative stress and autophagy in the central nervous system.

HSD17B1 Compensates for HSD17B3 Deficiency in Fetal Mouse Testis but Not in Adults.

Hydroxysteroid (17β) dehydrogenase (HSD17B) enzymes convert 17-ketosteroids to 17beta-hydroxysteroids, an essential step in testosterone biosynthesis. Human XY individuals with inactivating HSD17B3 mutations are born with female-appearing external genitalia due to testosterone deficiency. However, at puberty their testosterone production reactivates, indicating HSD17B3-independent testosterone synthesis.

DAPK1 mediates cognitive dysfunction and neuronal apoptosis in PSD rats through the ERK/CREB/BDNF signaling pathway.

Post-stroke depression (PSD) is one of the most common mental sequelae after a stroke and can damage the brain. Although PSD has garnered increasing attention in recent years, the precise mechanism remains unclear. Studies have indicated that the expression of DAPK1 is elevated in various neurodegenerative conditions, including depression, ischemic stroke, and Alzheimer's disease.

The histone lysine methyltransferase MLL1 regulates the activation and functional specialization of regulatory T cells.

The activation and specialization of regulatory T cells (Tregs) are crucial for maintaining immune self-tolerance; however, the regulation of these processes by histone modifications is not fully understood. Here, we show that T cell-specific deletion of the lysine methyltransferase MLL1 results in a spontaneous lymphocyte proliferation phenotype in aged mice without disturbing the development of conventional T cells and Tregs. Treg-specific MLL1 ablation leads to a systemic autoimmune disease associated with Treg dysfunction.

FTO Positively Regulates Odontoblastic Differentiation via SMOC2 in Human Stem Cells from the Apical Papilla under Inflammatory Microenvironment.

Odontoblastic differentiation of human stem cells from the apical papilla (hSCAPs) is crucial for continued root development and dentin formation in immature teeth with apical periodontitis (AP). Fat mass and obesity-associated protein (FTO) has been reported to regulate bone regeneration and osteogenic differentiation profoundly. However, the effect of FTO on hSCAPs remains unknown.

Recent progress in nanomaterials for bacteria-related tumor therapy.

Many studies suggest that tumor microbiome closely relates to the oncogenesis and anti-tumor responses in multiple cancer types (, colorectal cancer (CRC), breast cancer, lung cancer and pancreatic cancer), thereby raising an emerging research area of bacteria-related tumor therapy. Nanomaterials have long been used for both cancer and bacterial infection treatment, holding great potential for bacteria-related tumor therapy. In this review, we summarized recent progress in nanomaterials for bacteria-related tumor therapy.