Effects of a Dendritic Cell Vaccine Loaded With Whole Tumor Antigen on Bladder Cancer Model in hu-PBL-SCID Mice.

The aim of this study was to establish a humanized immune system model in severe combined immunodeficient (SCID) mice, assess dendritic cell (DC) phenotype, and evaluate the therapeutic efficacy of a DC-based vaccine in a bladder cancer model. Bladder cancer was induced in SCID mice by injection of T24 cells, followed by human peripheral blood leukocyte (hu-PBL) inoculation to reconstitute the human immune system. DCs were generated in vitro by culturing hu-PBL for 5 days and matured on the eighth day.

Mechanistic Study on the Inhibitory Effect of Dandelion Extract on Breast Cancer Cell Proliferation and Its Induction of Apoptosis.

This study aimed to investigate the underlying mechanisms by which dandelion extract inhibits the proliferation of breast cancer MDA-MB-231 cells. Dandelion root and leaf extracts were prepared using a heat reflux method and subjected to solvent gradient extraction to obtain fractions with different polarities. MTT assays revealed that the ethyl acetate fraction exhibited the strongest inhibitory effect on cell proliferation.

Research and advances in mouse models of diabetic nephropathy: a narrative review.

Diabetic nephropathy (DN) is a leading cause of end-stage renal disease (ESRD), and thus, appropriate animal models are critically needed to investigate its pathogenesis and identify new therapeutic targets. DN mouse models are important tools for studying the mechanisms of DN and exploring therapeutic strategies. Common features of the renal pathology in diabetic patients include thickening of the glomerular basement membrane, mesangial expansion, glomerulosclerosis, tubular injury, and interstitial fibrosis.

Effects of dual-task mode brain-computer interface based on motor imagery and virtual reality on balance and attention in patients with stroke: a randomized controlled pilot trial.

Background: Brain-computer interface (BCI) has been shown to be beneficial in improving lower limb motility in stroke, but their effectiveness on balance and attention is unclear. In addition, current BCIs are mostly in single-task mode. The BCI system used in this study was based on a dual-task model of motor imagery (MI) and virtual reality (VR).

T2T Genomes Unveil Centromere Architecture and Adaptive Divergence in Large Yellow Croaker (Larimichthys crocea).

The large yellow croaker (Larimichthys crocea) is a key aquaculture species, yet gaps in high-quality genomes hinder studies of centromeric and adaptive evolution. This study presents two telomere-to-telomere (T2T), gapless genomes from the Min-Yuedong (MYD) and Daiqu (DQ) populations, which diverged 4.18 million years ago.

Smoking status and the efficacy of remote ischaemic conditioning: a secondary analysis of the RICAMIS trial.

Background: Remote ischaemic conditioning (RIC) is a potential non-invasive neuroprotective strategy, but it remains unclear whether its efficacy is influenced by smoking status. This study explored the impact of smoking status on the therapeutic effect of RIC in patients with acute moderate ischaemic stroke through a post hoc subgroup analysis.

Methods: Based on the data from the Remote Ischemic Conditioning for Acute Moderate Ischemic Stroke trial, 1717 patients with moderate stroke (National Institutes of Health Stroke Scale score 6-16) who did not undergo reperfusion therapy were included and categorised into current smokers (n=505) and non-smokers (n=1212) based on their smoking status.

Dual Antiplatelet Therapy Versus Alteplase for Mild Stroke with Admission NIHSS Score 0-3 Versus 4-5: A Secondary Analysis of the ARAMIS Randomized Trial.

Background: The ARAMIS trial demonstrated the non-inferiority of dual antiplatelet therapy (DAPT) to alteplase in patients with minor nondisabling ischemic stroke. We aimed to investigate whether the degree of neurological deficit can affect the benefit of DAPT versus alteplase.

Methods: On the basis of the as-treated analysis set, eligible patients with acute minor nondisabling stroke were divided into NIHSS 0-3 and NIHSS 4-5 groups, which was further subdivided into DAPT and alteplase groups.

The Impact of Informal Digital Learning of English (IDLE) on EFL Learners' Engagement: Mediating Roles of Flow, Online Self-Efficacy, and Behavioral Intention.

In the evolving landscape of online language learning, informal digital learning of English (IDLE) plays a crucial role, particularly among English as a foreign language (EFL) learners. Previous research has investigated the direct impact of IDLE on EFL learners' engagement. However, little attention has been given to the underlying mechanisms that drive this relationship.

Assessing the biomechanics of scheuermann's kyphosis affected thoracolumbar spine in forward flexion at the tissue-level using a finite element model.

Thoracolumbar kyphosis (TLK) secondary to Scheuermann's disease often leads to low back pain, which may be related to altered biomechanical properties of the spine. However, There is a lack of biomechanical studies in the literature that comprehensively evaluate tissue-level stresses and strains in the thoracolumbar spine affected by Scheuermann's kyphosis, particularly during functional motions such as forward flexion. This study analyzed biomechanical changes during forward flexion in TLK patients using musculoskeletal dynamics and finite element modeling.

Surgical Correction for Pediatric Epiblepharon and Trichiasis.

Lower eyelid epiblepharon is a condition that is common in Asian children and improves with age in most patients; however, the problem can persist until school age or even adulthood. No difference has been observed in terms of incidence in association with sex or the affected eye, and the condition mainly affects the lower eyelid, usually in both eyes. The condition typically features horizontal semilunar skin folds lying parallel to the palpebral margin that accumulate and push the eyelashes towards the ocular surface, which rub against the cornea, resulting in corneal injury and irritation symptoms that affect the daily life of the child.

Dynamic changes in flavonoid profiles and their bioactive properties during rice yellowing.

Rice readily undergoes yellowing under heat stress. Traditionally viewed as a sign of quality decline, this phenomenon is concurrent with the accumulation of flavonoids. Consequently, our study optimized flavonoid extraction (60-min ultrasound, 40-min shaking time, 50 g/mL solid-liquid ratio) and analyzed yellowing-stage dynamics.

Senescence of dental pulp stem cells: phenotypes, underlying mechanisms and regulatory molecules.

Dental pulp stem cells (DPSCs) are a population of adult stem cells with self-renewal capacity and multilineage differentiation potential, widely utilized in tissue engineering and regenerative medicine. However, with increasing donor age or prolonged in vitro expansion, DPSCs gradually exhibit senescence-associated phenotypes, including reduced proliferative capacity and impaired differentiation potential. This review comprehensively summarizes current advances in the study of DPSCs senescence.

Optimized DWI-based virtual MR elastography for diagnosis and therapeutic monitoring of focal liver lesions.

Objectives: This study aimed to develop and evaluate an optimized DWI-based virtual MR elastography (vMRE) technique for diagnosing focal liver lesions (FLLs) and monitoring therapeutic responses in malignant tumors, comparing its performance with traditional MRE.

Materials And Methods: This retrospective analysis of 142 patients with FLLs using six b-value DWI (150-1500 s/mm2) to calculate eight apparent diffusion coefficient (ADC) combinations. Linear regression analysis was performed to evaluate the correlation between ADC and traditional MRE, and a vMRE formula was constructed.

Efficacy of argatroban plus alteplase according to time from onset to thrombolysis in acute ischemic stroke: a prespecified analysis of the ARAIS trial.

Background: The Argatroban Plus Recombinant Tissue-Type Plasminogen Activator for AIS (ARAIS) trial failed to demonstrate the benefit of argatroban in patients diagnosed with acute ischemic stroke who were treated with intravenous alteplase. A analysis was conducted to investigate whether the time from onset to thrombolysis (OTT) affected the outcomes.

Methods: The included patients were classified into two subgroups according to OTT.

Effect of the Kickstart exoskeleton lower extremity walking system on improving lower extremity walking ability in subacute stroke patients: a randomized controlled trial.

Background: Walking dysfunction is a primary cause of a reduced ability to perform activities of daily living and decreased quality of life in stroke patients. The Kickstart Walk assist system is portable and easy to don and remove. There is a lack of high-quality, randomized controlled trials to validate its effectiveness.

Analysis of influencing factors on recurrence and metastasis of pT1b ~ pT4aNOM0 esophageal squamous cell carcinoma after radical surgery in Northeast Sichuan region.

Background: The incidence and mortality rates of esophageal cancer are high, yet public awareness of this disease remains low, particularly in the northeastern Sichuan region. This study aims to investigate the factors influencing recurrence and metastasis after radical surgery for pT1b ~ pT4aN0M0 esophageal squamous cell carcinoma in northeastern Sichuan, providing a personalized reference for postoperative follow-up of patients undergoing radical esophagectomy for esophageal squamous cell carcinoma.

Methods: A retrospective analysis was conducted on the clinical data of 151 patients with pT1b to pT4aN0M0 esophageal squamous cell carcinoma who underwent radical surgery in the northeastern Sichuan region.

Platelet-to-Neutrophil Ratio and Efficacy of Remote Ischemic Conditioning in Acute Ischemic Stroke.

Background: Remote ischemic conditioning (RIC) has been proven to improve neurologic function after stroke in RICAMIS trial. Aggravated thrombosis and inflammatory through interaction between platelet and immune cells affected the prognosis of stroke. We conducted an exploratory secondary analysis of RICAMIS to investigate whether platelet-to-neutrophil ratio (PNR) predicted efficacy of RIC in stroke.

Integrative multi-omics and bioinformatics analysis of the effects of BaiRui YuPingFeng Powder on intestinal health in broilers.

Introduction: In recent years, global poultry consumption has increased rapidly, making chicken the most widely consumed meat worldwide by 2019. To increase livestock development, antibiotics are often added to animal feed as growth promoters. But overuse of antibiotics may alter the gut microbiota, make people more resistant to them, and raise the possibility that they will spread antibiotic resistance genes to the human microbiome.

Imaging and Tailoring Chemical Evolution Kinetics of (0001) Facet on Single β-Co(OH) Nanoplates for the Electrocatalytic Oxygen Evolution Reaction.

The (0001) crystal facet of β-Co(OH) has been widely accepted as an inert facet for the oxygen evolution reaction (OER), while the (101̅0) facet is considered more active. However, mechanistic details regarding the origin of the differences at the coordination environment and the electronic state level remain unexplored to date. Herein, we used a multimode optical imaging method to track the evolving heterogeneous dynamics of cobalt species during the OER process and correlate it with the OER performance.

Machine learning model for the prediction of postoperative acute kidney injury (AKI) in neonates undergoing digestive surgery.

Objective: The aim of our study is to determine the main predictors of postoperative AKI in neonates using machine learning models compared with the logistic regression model.

Methods: Demographic and pre-operative data of 742 neonates who underwent digestive surgery were collected during this retrospective study. Data were randomly split into training and testing datasets at a ratio of 7:3.

Dynamic formation tracking and fault-tolerant control of multi-agent systems based on distance and topology reconstruction methods.

This paper introduces a distributed approach for dynamic formation tracking and formation fault-tolerant control within the port-Hamiltonian energy framework for multi-agent system (MAS) affected by Coulomb friction. The coupling relationships between agents are equivalently modeled as virtual springs, which simulate the interaction forces between agents to reflect the relative positions and motion states of the agents. A distance-based distributed control scheme is designed, to ensure that the formation composed of multiple agents can continuously adjust the direction and size of the formation while achieving target tracking.

[Interpretation of the WHO's "Ethics and Governance of Artificial Intelligence for Health： Guidance on Large Multi-Modal Models" and its implications for China].

With the rapid advancement and iterative development of new artificial intelligence technologies, there remains a regulatory vacuum in corresponding governance measures among governments worldwide. Simultaneously, a technological and governance gap exists between developing countries and developed economies. In response, the World Health Organization (WHO) has released "Ethics and Governance of Artificial Intelligence for Health: Guidance on Large Multi-Modal Models" to assist governments in strengthening governance capabilities in this field.

Efficacy of Cylindrical Annular Refractive Elements (CARE) Spectacle Lenses in Slowing Myopia Progression Over 2 Years.

Purpose: To evaluate over a 2-year period the efficacy of spectacle lenses incorporating cylindrical annular refractive elements (CARE) in slowing myopia progression compared with single vision (SV) spectacle wear.

Design: Double-masked, multicenter, randomized clinical trial.

Methods: A total of 240 Chinese children aged 6 to 13 years, with a spherical equivalent (SE) refractive error of -0.

Application of multi-objective optimization algorithm to the preparation of polycaprolactone microsphere formulations.

Background: Polycaprolactone microspheres (PCL-MS) are widely used in tissue filling, while particle size and uniformity are crucial for improving filling and therapeutic effects. Efficient optimization methods are essential for accelerating their development.

Methods: Box-Behnken design was applied to investigate three factors for PCL-MS: PCL concentration (X), polyvinyl alcohol concentration (X) and water-oil ratio (WOR,X).