T2T Genomes Unveil Centromere Architecture and Adaptive Divergence in Large Yellow Croaker (Larimichthys crocea).

The large yellow croaker (Larimichthys crocea) is a key aquaculture species, yet gaps in high-quality genomes hinder studies of centromeric and adaptive evolution. This study presents two telomere-to-telomere (T2T), gapless genomes from the Min-Yuedong (MYD) and Daiqu (DQ) populations, which diverged 4.18 million years ago.

Recyclable chemical-enzymatic hydrolysis cascade for the degradation and closed-loop recycling of blended fabrics.

The annual increase in textile consumption has led to significant waste generation, resulting in severe environmental pollution and resource waste. Particularly for the most common polyester/cotton blended fabrics, the composition is intricate, and recycling is even more challenging. In this study, a recyclable chemical-enzymatic hydrolysis cascade was developed to degrade and achieve closed-loop recycling of blended fabrics.

An in vivo target mutagenesis system for multiple hosts.

In vivo target mutagenesis is a powerful approach to accelerate protein evolution. However, current approaches have been primarily developed in conventional organisms, limiting their capacity to evolve proteins with subtle variations across non-conventional host species. Here, we design an in vivo target mutagenesis system for multiple hosts (ITMU) utilizing the broad host-range plasmid RSF1010 replication element.

A rapid and efficient strategy for combinatorial repression of multiple genes in Escherichia coli.

Background: The regulation of multiple gene expression is pivotal for metabolic engineering. Although CRISPR interference (CRISPRi) has been extensively utilized for multi-gene regulation, the construction of numerous single-guide RNA (sgRNA) expression plasmids for combinatorial regulation remains a significant challenge.

Results: In this study, we developed a combinatorial repression system for multiple genes by optimizing the expression of multi-sgRNA with various inducible promoters in Escherichia coli.

Single-crystal ZrCo nanoparticle for advanced hydrogen and H-isotope storage.

Hydrogen-isotope storage materials are essential for the controlled nuclear fusion. However, the currently used smelting-ZrCo alloy suffers from rapid degradation of performance due to severe disproportionation. Here, we reveal a defect-derived disproportionation mechanism and report a nano-single-crystal strategy to solve ZrCo's problems.

Upcycling of PET oligomers from chemical recycling processes to PHA by microbial co-cultivation.

Polyethylene terephthalate (PET) is the most widely consumed polyester plastic and can be recycled by many chemical processes, of which glycolysis is most cost-effective and commercially viable. However, PET glycolysis produces oligomers due to incomplete depolymerization, which are undesirable by-products and require proper disposal. In this study, the PET oligomers from chemical recycling processes were completely bio-depolymerized into monomers and then used for the biosynthesis of biodegradable plastics polyhydroxyalkanoates (PHA) by co-cultivation of two engineered microorganisms Escherichia coli BL21 (DE3)-LCC and Pseudomonas putida KT2440-ΔRDt-ΔZP46C-M.

Unique Raoultella species isolated from petroleum contaminated soil degrades polystyrene and polyethylene.

Polyolefin plastics, such as polyethylene (PE) and polystyrene (PS), are the most widely used synthetic plastics in our daily life. However, the chemical structure of polyolefin plastics is composed of carbon-carbon (C-C) bonds, which is extremely stable and makes polyolefin plastics recalcitrant to degradation. The growing accumulation of plastic waste has caused serious environmental pollution and has become a global environmental concern.

[Advances in biodegradation of polyolefin plastics].

Polyolefin plastics are a group of polymers with C-C backbone that have been widely used in various areas of daily life. Due to their stable chemical properties and poor biodegradability, polyolefin plastic waste continues to accumulate worldwide, causing serious environmental pollution and ecological crises. In recent years, biological degradation of polyolefin plastics has attracted considerable attention.

Biodegradation of polyurethane by the microbial consortia enriched from landfill.

Polyurethanes (PU) are one of the most used categories of plastics and have become a significant source of environmental pollutants. Degrading the refractory PU wastes using environmentally friendly strategies is in high demand. In this study, three microbial consortia from the landfill leachate were enriched using PU powder as the sole carbon source.

Honeycomb ZrCo Intermetallic for High Performance Hydrogen and Hydrogen Isotope Storage.

Hydrogen isotope storage materials are of great significance for controlled nuclear fusion, which is promising to provide unlimited clean and dense energy. Conventional storage materials of micrometer-sized polycrystalline ZrCo alloys prepared by the smelting method suffer from slow kinetics, pulverization, disproportionation, and poor cycling stability. Here, we synthesize a honeycomb-structured ZrCo composed of highly crystalline submicrometer ZrCo units using electrospray deposition and magnesiothermic reduction.

Valorization of Polyethylene Terephthalate to Muconic Acid by Engineering .

Plastic waste is rapidly accumulating in the environment and becoming a huge global challenge. Many studies have highlighted the role of microbial metabolic engineering for the valorization of polyethylene terephthalate (PET) waste. In this study, we proposed a new conceptual scheme for upcycling of PET.

Characterization of Salmonella endolysin XFII produced by recombinant Escherichia coli and its application combined with chitosan in lysing Gram-negative bacteria.

Background:  Salmonella is a common foodborne pathogen, which can cause intestinal diseases. In the last decades, the overuse of antibiotics has led to a pandemic of drug-resistant bacterial infections. To tackle the burden of antimicrobial resistant pathogens, it is necessary to develop new antimicrobial drugs with novel modes of action.

Enhancing thermal stability and lytic activity of phage lysin PlyAB1 from Acinetobacter baumannii.

With the increasingly serious drug resistance of Acinetobacter baumannii, there is an increasingly urgent need for new antibacterial drugs. Phage lysin PlyAB1 has a bactericidal effect on drug-resistant A. baumannii, which has the potential to replace antibiotics to fight infection caused by A.

ICGRM: integrative construction of genomic relationship matrix combining multiple genomic regions for big dataset.

Background: Genomic prediction is an advanced method for estimating genetic values, which has been widely accepted for genetic evaluation in animal and disease-risk prediction in human. It estimates genetic values with genome-wide distributed SNPs instead of pedigree. The key step of it is to construct genomic relationship matrix (GRM) via genome-wide SNPs; however, usually the calculation of GRM needs huge computer memory especially when the SNP number and sample size are big, so that sometimes it will become computationally prohibitive even for super computer clusters.