Innovative applications of plasma-activated water in Chinese mitten crab (Eriocheir sinensis): Improving quality, safety and sustainability.

This study explores plasma-activated water (PAW) as a sustainable aquaculture water treatment for Chinese mitten crab (Eriocheir sinensis), evaluating its impact on antioxidant responses, microbial dynamics, and crab quality. We evaluated the physiological and microbial responses of E. sinensis using high-throughput sequencing and antioxidant enzyme assays.

A turn-on supramolecular probe for visible detection and accurate identification of phenethylamine with orange emission.

The supramolecular fluorescent probe exhibited promising application in the field of detection and analysis, but developing supramolecular fluorescent probes with long wavelength emission and enhancement functions for the detection of phenylethylamines is a challenge. In this work, the guest molecule SPn with a D-π-A structure was designed and synthesized, and supramolecular fluorescent probe (SPn/Q[8]) with long wavelength emission was constructed by the host-guest interaction with cucurbit[8]uril (Q[8]). This probe detects phenethylamines based on an indicator displacement assay, which is a process characterized by a fast effect rate (within 6.

Systemic IFN-I Synergizes with Topical TLR7/8 Agonists to Suppress Metastatic Tumors.

Advancing targeted cancer immunotherapy is pivotal for overcoming distant metastasis and tumor relapse. The recent study in by Sanlorenzo et al. demonstrates a breakthrough strategy combining systemic type I interferon with topical Toll-like receptor 7/8 agonists, where oral imiquimod primes plasmacytoid dendritic cells (DCs) to produce type I interferon, thereby sensitizing conventional DCs in tumors to local Toll-like receptor 7 activation.

Sequential Separation of Metal Cations Using Nor-seco-cucurbit[10]uril as a Supramolecular Material.

The treatment of metal-bearing wastewater is a critical step in safeguarding aquatic ecosystems and human health. In this study, the coordination characteristics of --cucurbit[10]uril (-Q[10]) were systematically examined with various metal cations, encompassing alkali metal, alkaline earth metal, and rare earth metals, in HCl aqueous solution. Through X-ray single-crystal diffraction analysis, 12 distinct -Q[10]-based coordination complexes were identified, incorporating Ca, Y, La, Ce, Pr, Sm, Eu, Gd, Tb, Dy, Ho, and Er metal ions.

Targeting One Precise Aggregation-Induced Emission (AIE) Mechanism to Design Indolocarbazole-Based AIE-Active Phosphorescent Pt(II) Complex and Its Anion Sensing Property.

AIE mechanisms of the restrained intramolecular rotation (RIR) and restrained molecular configuration transformation (RMCT) jointly contribute to the AIE nature of the Pt(II)(C^N)(N-donor ligand)Cl-type complex. However, focusing on one certain mechanism would provide more accessibilities to AIE molecular designs. Herein, by combining a rigid cyclometallating ligand 11,12-dibutyl-3-(pyridin-2-yl)-11,12-dihydroindolo[2,3-]carbazole (ICZ--Py) with a rotational monodentate ligand 2-hydroxy-4-(pyridin-4-yl)benzaldehyde (L-SYQ), an AIE-active Pt(II) complex receiving rigid coordination configuration of the Pt center is designed and synthesized.

Regenerative growth in Eriocheir sinensis is driven by Slc7a5-TORC1-regulated cell signaling.

Limb regeneration is a widespread phenomenon among animals, yet the underlying molecular mechanisms remain incompletely understood. Crustaceans like the Chinese mitten crab (Eriocheir sinensis), possess the remarkable ability to regrow lost limbs through a highly regulated regenerative process. In this study, we investigated the molecular mechanisms underlying limb regeneration in E.

Comparative Analysis of Transposable Element Evolution in Crustaceans.

Transposable elements (TEs) are pivotal in driving genome evolution, playing a significant role in generating genetic diversity, modulating genome size, and inducing chromosome structural changes. Despite extensive research in model species, studies on TE evolution and structure in crustaceans are notably limited. This study offers an in-depth analysis of TEs diversity and its impact on representative crustacean genomes' genome structure and function.

Manipulation mechanisms of bubble aggregation and evolution in inertial cavitation fields.

Spherical bubble clusters was observed at 28 kHz and 40 kHz, and the evolution of the clusters was investigated. It was found that the cluster was dense when it located at the antinode of standing waves, while it became sparse when it deviates from the antinode, and the bifurcation of period doubling was observed in this nonlinear bubble system. In clusters, there exists complex fragmentation and coalescence, implying a bubble transportation cycle inside the clusters, which may enhance the interaction between the cluster and surrounding tiny bubbles.

Draft genome assembly of the largemouth bass (Micropterus salmoides).

Objective: Largemouth bass (Micropterus salmoides, LMB) is an important species in aquaculture, and the annual production is rapidly increasing. Genetic and breeding studies related to LMB have promising applications, and a high-quality genome assembly is essential for interpreting genetic and sequencing data. In this study, we sequenced the genome of a male LMB using the PacBio Sequel platform, high-throughput chromosome conformation capture (Hi-C), and paired-end Illumina sequencing.

The oscillations of non-spherical bubbles in liquid.

In this paper, the interaction between non-spherical bubbles is studied using a high-speed camera, and the effects of the interaction on the temperature within the bubble and the velocity of the surrounding fluid are theoretically investigated. It is found that the mean radius and the mean wall velocity of the middle bubble in three-bubble system are slightly greater than those in two-bubble system when the initial parameters are consistent. The acoustic response of the middle bubble presented a leftward shift of resonance peak and an increase of resonance peaks with increasing sound pressure.

Fish gut microbiome and its application in aquaculture and biological conservation.

Understanding the diversity and function of fish gut microbiomes has advanced substantially, yet many aspects remain poorly understood, particularly the interplay among microbiota, host species, and environmental factors in the context of conservation. This review explores the composition and abundance of gut bacterial communities in key aquaculture fish groups-cyprinids, ictalurids (catfish), salmonids, and cichlids (tilapia)-alongside the model organism zebrafish, across diverse geographic regions. The findings highlight environmental habitats and host species as primary determinants of gut microbiome structure, offering a global perspective on these microbial communities.

Comparative the effect of bisphenol A and bisphenol S on the development and spectral sensitivity of cone photoreceptors in zebrafish larvae (Danio rerio).

Color vision, which is mediated by cone photoreceptors in vertebrates, is essential for perceiving the external environment. Bisphenol A (BPA) and its substitute bisphenol S (BPS) have been widely used worldwide, while the evaluation of their safety, especially the newly discovered visual toxicity mechanism caused by them in recent years, has not been clearly explored. In the present study, we investigated the effects of BPA treatment (1, 10, and 100 μg/L) on cone cell development and function to evaluate visual toxicity.

Supramolecular phosphorescent assemblies based on cucurbit[8]uril and bromophenylpyridine derivatives for dazomet recognition.

A bromophenylpyridine derivative (N1) was designed, synthesized, and the molecule was incorporated into the cavity of the cucurbit[8]uril (Q[8]) as a guest to form a 2:1 host-guest complex. This complex demonstrates good room temperature phosphorescence (RTP) properties in aqueous solution. The host-guest interaction and optical properties of N1@Q[8] in aqueous solution were studied by means of H NMR, ultraviolet-visible absorption spectroscopy, fluorescence spectroscopy, phosphorescence spectroscopy, scanning electron microscopy and inverted fluorescence microscopy.

PLK1 inhibition impairs erythroid differentiation.

Polo-like kinase 1 (PLK1), a key regulator of the G2/M phase in mitosis, is frequently overexpressed in numerous tumors. Although PLK1 inhibitors have emerged as promising therapeutic agents for cancer, their use has been linked to significant anemia in a subset of patients, yet the underlying mechanisms remain poorly understood. In this study, we utilized an human umbilical cord blood-derived CD34 cell-based erythroid differentiation system, alongside a murine model, to investigate the impact of PLK1 inhibitors on erythropoiesis.

Effects of short-term salt exposure on gill damage, serum components and gene expression patterns in juvenile Largemouth bass (Micropterus salmoides).

The Largemouth bass (Micropterus salmoides; LMB) is a freshwater fish that plays a significant role in aquaculture, and its cultural base is expanding into inland saline water areas. To study the effect of short-term salt exposure on LMB, fish with an average body weight of 11.69 (±1.

Mutation of CRYAB encoding a conserved mitochondrial chaperone and antiapoptotic protein causes hereditary optic atrophy.

The degeneration of retinal ganglion cells (RGC) due to mitochondrial dysfunctions manifests optic neuropathy. However, the molecular components of RGC linked to optic neuropathy manifestations remain largely unknown. Here, we identified a potentially novel optic atrophy-causative CRYAB gene encoding a highly conserved major lens protein acting as mitochondrial chaperone and possessing antiapoptotic activities.

Evolutionary mechanism of Y-branches in acoustic Lichtenberg figures just below the water surface.

The acoustic Lichtenberg figure (ALF) in an ultrasonic cleaner with a frequency of 28 kHz at different power levels was observed using high-speed photography. The nonlinear response of the cavitation structure was analyzed by the entropy spectrum in the ALF images, which showed the modulation influence of the primary acoustic field, exhibiting the fluctuations of the bubble distribution with time. Typical Y-branches predict the paths by which surrounding bubbles are attracted and converge into the structure, the branches are curved due to bubble-bubble interactions, and the curvature increases as the bubbles are approaching the main chain.

[Source Profiles of VOCs for Different Types of Industrial Boilers in Sichuan, China].

Seven different types of industrial boilers in Sichuan Province were selected to determine the VOC emission components and the source profiles of VOCs containing 115 components were established using Teflon sampling and GC-MS/FID analysis. The ozone formation potential （OFP） and emission factors of VOCs from different types of industrial boilers were analyzed. The results showed that the VOC components emitted from different types of industrial boilers were different.

Acoustic black hole immersed sonoreactor for high-efficiency cavitation treatment.

Developing innovative sonoreactors to enhance acoustic processing efficiency holds immense importance in the field of sonochemistry. Traditional immersed sonoreactors (TISs) mainly produce cavitation at the probe tip, with a relatively weak cavitation around the probe, resulting in posing challenges for high-efficiency cavitation treatment. Here we propose an acoustic black hole immersed sonoreactor (ABHIS) in longitudinal-flexural coupled vibration, enabling high-efficiency cavitation treatment by unleashing the cavitation potential of the probe.

Gut Microbiome-Driven metabolites influence skin pigmentation in TYRP1 mutant Oujiang Color Common Carp.

The gut microbiome plays a key role in regulating the gut-skin axis, and host genetics partially influence this regulation. The study investigated the role of gut microbiota and host genetics in the gut-skin axis, focusing on the unusual "coffee-like" color phenotype observed in TYRP1 mutant Oujiang Color Common Carp. We employed comparative high-throughput omics data from wild-type and mutant fish to quantify the influence of both genetics and gut microbes on skin transcriptomic expression and blood metabolites.

Application value of adrenal venous sampling in the surgical treatment of primary aldosteronism.

The diagnosis of primary aldosteronism (PA) is critical for determining treatment strategies. The aim of the present study was to investigate the clinical value of preoperative adrenal venous sampling (AVS) for determining the benefit of PA surgery. Patients diagnosed with PA at Liaocheng People's Hospital (Liaocheng, China) between January 2015 and December 2020 were selected and divided into two groups: Group A underwent adrenal computed tomography (CT) only, whereas Group B underwent adrenal CT and successful AVS.