A novel synergistic regulatory mechanism involving the MYB39-MYB111-bHLH51-TTG1 module in the phenolic and diterpenoid biosynthetic pathways of Salvia miltiorrhiza.

Plants produce a variety of secondary metabolites, and their biosynthesis is often regulated by multiple transcription factors. However, the potential connections between the biosynthesis of different types of metabolites remain unclear. In this study, we present a novel mechanism in which the MYB39-MYB111-bHLH51-TTG1 module synergistically regulates both the phenolic and diterpenoid biosynthetic pathways in the medicinal model plant Salvia miltiorrhiza.

Comparative transcriptomic analysis and genome-wide identification provide insights into the potential role of fungal-responsive MAPK cascade genes in tanshinone accumulation in .

is a well-known traditional Chinese medicine (TCM) for its bioactive tanshinones that are used to treat various diseases and have high antimicrobial properties. Previous studies have shown that tanshinone accumulation in was shown to be significantly induced by fungal elicitors. Mitogen-activated protein kinases (MAPKs), which play critical roles in plant-microbe interactions and cellular processes, are known to regulate the accumulation of antimicrobial metabolites.

The Critical Role of Phenylpropanoid Biosynthesis Pathway in Lily Resistance Against Gray Mold.

Gray mold caused by is one of the most determinative factors of lily growth and has become a major threat to lily productivity. However, the nature of the lily interaction remains largely unknown. Here, comparative transcriptomic and metabolomic were used to investigate the defense responses of resistant ('Sorbonne') and susceptible ('Tresor') lily cultivars to infection at 24 hpi.

Metabolome and transcriptome analyses provide new insights into the mechanisms underlying the enhancement of medicinal component content in the roots of (Rupr. et Maxim.) Harms through foliar application of zinc fertilizer.

(Rupr. et Maxim.) Harms is a perennial shrub of the genus in the Araliaceae family and has a high medicinal value.

Unveiling the distribution of chemical constituents at different body parts and maturity stages of Ganoderma lingzhi by combining metabolomics with desorption electrospray ionization mass spectrometry imaging (DESI).

Ganoderma lingzhi is an important medicinal fungus, which is widely used as dietary supplement and for pharmaceutical industries. However, the spatial distribution and dynamic accumulation pattern of active components such as ganoderic acids (GAs) among different parts of G. lingzhi fruiting body are still unclear.

Metabolomic and antioxidant analyses of Bunge and Hemsl. seeds.

and seeds are rich in metabolites that are beneficial to human health and can be utilised as nutritional supplements. In this study, UPLC-MS and GC-MS based on extensively focused metabolomics were used to compare the seed metabolomics of the two species. LC-MS detected 118 metabolites, primarily Lipids and phenylpropanoids.

Germplasm resources and secondary metabolism regulation in Reishi mushroom ().

is a valuable medical macrofungus with a myriad of diverse secondary metabolites, in which triterpenoids are the major constituents. This paper introduced the germplasm resources of genus from textual research, its distribution and identification at the molecular level. Also we overviewed in the components, the biological activities and biosynthetic pathways of ganoderic acid, aiming to provide scientific evidence for the development and utilization of germplasm resources and the biosynthesis of ganoderic acid.

LC-MS metabolomics profiling of Salvia aegyptiaca L. and S. lanigera Poir. with the antimicrobial properties of their extracts.

Background: Salvia L. (Lamiaceae) found in almost all countries in temperate and tropical regions. Both S.

The complete chloroplast genome sequence of E.Peter, 1936 (Lamiaceae).

E.Peter is a medicinal herb mainly distributed in Huangshan Mountain of Anhui province, China. In this study, the first complete chloroplast genome of was sequenced and assembled.

Metabolite Profiles Provide Insights into Underlying Mechanism in (Apiaceae) in Response to Three Levels of Phosphorus Fertilization.

Phosphorus (P) deficiency affects plant yield and quality, yet at the same time, excessive phosphorus application does not necessarily promote the growth of plants. How to maintain a balance between biomass accumulation and phosphorus application is a problem. Therefore, the purpose of this research was to explore the relationship between yield and quality of and phosphorus fertilization, based on three phosphorus fertilization levels (20 kg∙ha; 10 kg∙ha; and 0 kg∙ha).

UV-B Radiation Largely Promoted the Transformation of Primary Metabolites to Phenols in Seedlings.

Ultraviolet-B (UV-B) radiation (280-320 nm) may induce photobiological stress in plants, activate the plant defense system, and induce changes of metabolites. In our previous work, we found that between the two varieties prescribed by the Chinese Pharmacopoeia, has better tolerance to UV-B. Thus, it is necessary to study the metabolic strategy of under UV-B radiation further.

Comparative metabolomics of two saline-alkali tolerant plants and based on GC-MS platform.

and are two important saline-alkali tolerant plants that can improve the soil properties. For exploring the different tolerance mechanisms between them, GC-MS-based metabolomics was used to comprehensively evaluate the primary metabolites differences, a total of 51 different metabolites were present in different quantities. The identified compounds were mainly 11 sugars, 7 amino acids, 5 alcohols and 18 organic acids; they play an important role in responding to the saline-alkali stress and distinguish between and .

Metabolomics Analysis Reveals that Ethylene and Methyl Jasmonate Regulate Different Branch Pathways to Promote the Accumulation of Terpenoid Indole Alkaloids in Catharanthus roseus.

The medicinal plant Catharanthus roseus accumulates large numbers of terpenoid indole alkaloids (TIAs), including the pharmaceutically important vinblastine, vincristine, ajmalicine, and serpentine. The phytohormone ethylene or methyl jasmonate (MeJA) can markedly enhance alkaloid accumulation. The interaction between ethylene or MeJA in the regulation of TIA biosynthesis in C.

Seed metabolite profiling of Vicia species from China via GC-MS.

In this study, we examined Vicia seeds using gas chromatography-mass spectrometry (GC-MS). The metabolic differences of seeds of twelve Vicia species were assessed. 184 metabolites were identified.

The Different Resistance of Two Astragalus Plants to UV-B Stress is Tightly Associated with the Organ-specific Isoflavone Metabolism.

In this work, the changes in isoflavone levels and the expression of genes involved in their biosynthesis were studied in two Astragalus by UPLC-MS and real-time PCR after 10 days of UV-B treatment (λ  = 313 nm, 804 J m ). Isoflavones were significantly induced by UV-B irradiation. The influence might be activated by the regulation of these target genes.

GC-MS Metabolomic Analysis to Reveal the Metabolites and Biological Pathways Involved in the Developmental Stages and Tissue Response of Panax ginseng.

Ginsenosides, the major compounds present in ginseng, are known to have numerous physiological and pharmacological effects. The physiological processes, enzymes and genes involved in ginsenoside synthesis in have been well characterized. However, relatively little information is known about the dynamic metabolic changes that occur during ginsenoside accumulation in ginseng.

Ethylene Improves Root System Development under Cadmium Stress by Modulating Superoxide Anion Concentration in .

This work aims at identifying the effects of ethylene on the response of root system to cadmium chloride (CdCl) stress. Two ethylene-insensitive mutants, and , were subjected to (25, 50, 75, and 100 μM) CdCl concentrations, from which 75 μM concentration decreased root growth by 40% compared with wild type Col-0 as a control. Ethylene biosynthesis increased in response to CdCl treatment.

The integration of GC-MS and LC-MS to assay the metabolomics profiling in Panax ginseng and Panax quinquefolius reveals a tissue- and species-specific connectivity of primary metabolites and ginsenosides accumulation.

The traditional medicine Ginseng mainly including Panax ginseng and Panax quinquefolius is the most widely consumed herbal product in the world. Despite the extensive investigation of biosynthetic pathway of the active compounds ginsenosides, our current understanding of the metabolic interlink between ginsenosides synthesis and primary metabolism at the whole-plant level. In this study, the tissue-specific profiling of primary and the secondary metabolites in two different species of ginseng were investigated by gas chromatography- and liquid chromatography coupled to mass spectrometry.

Simultaneous determination of six active metabolites in Astragalus mongholicus (Fisch.) Bge. under salt stress by ultra-pressure liquid chromatography with tandem mass spectrometry.

Astragalus membranaceus Bge. var. mongholicus (Bge.