Synergistic inhibition of autophagic flux and induction of apoptosis in cervical cancer cells by Mito-TEMPO and hyperthermia.

Background: Hyperthermia (HT), while a cancer treatment approach, isn't always effective alone. Therefore, identifying hyperthermia enhancers is crucial. We demonstrated that Mito-TEMPO ([2-[(1-Hydroxy-2,2,6,6-tetramethylpiperidin-4-yl) amino]-2-oxoethyl]-triphenylphosphanium, MT) acts as a potent thermosensitizer, promoting cell death in human cervical cancer (HeLa) cells.

In vivo efficacy of fidaxomicin against rpoB mutant Clostridioides difficile infection.

Objectives: Clostridioides difficile infection (CDI) is a well-known healthcare-associated diarrheal disease. Fidaxomicin, a key antibiotic used to treat CDI, targets bacterial RNA polymerase. However, some clinical isolates have mutations in rpoB, which reduces their susceptibility to this antibiotic.

FMR1 mutant marmosets show fragile X syndrome phenotypes.

Fragile X syndrome (FXS) is the foremost monogenic cause of autism spectrum disorder and intellectual disability, caused by FMR1 gene silencing. Here, we report that common marmosets carrying FMR1 mutation, a non-human primate model for FXS, share common features in behavioral and molecular phenotypes with patients with FXS. Founder mutants with markedly reduced fragile X messenger ribonucleoprotein expression display hyperactivity, spontaneous seizures, and transcriptome changes in synapse-related genes that overlap with those reported in patients with FXS.

Risk factors for moderate/severe bronchopulmonary dysplasia: A retrospective cohort study including results of an accurate assessment of intra-uterine microbes.

A highly sensitive PCR method developed in our university accurately identifies the presence or absence of intra-uterine (IU) microbes without false positive results. With the inclusion of the results of an accurate assessment of IU microbes, risk factors for the development of moderate/severe bronchopulmonary dysplasia (BPD), a chronic lung disease that affects premature infants who require prolonged oxygen therapy or medical ventilation, were examined in 107 spontaneous preterm neonates. Perinatal risk factors were compared between cases of moderate/severe BPD (N = 49) and mild/non-BPD (N = 58).

A Case of Advanced Urothelial Carcinoma Requiring Treatment Following a Positive Interferon-Gamma Release Assay Prior to Avelumab Administration.

Introduction: The association between the risk of latent tuberculosis infection (LTBI) reactivation and immune checkpoint inhibitor (ICI) administration has been reported.

Case Presentation: A man in his seventies underwent robot-assisted laparoscopic radical cystectomy with ileal conduit diversion for muscle-invasive bladder cancer. Three years postoperatively, CT revealed metastases to the para-aortic lymph nodes and rectum.

Rapid and long-lasting antidepressant-like effects of the pituitary adenylate cyclase-activating polypeptide receptor antagonist PA-915 in chronic stress mouse models.

Stress-related disorders, such as depression and anxiety, have been one of the most important medical issues. Accumulating evidence suggests that the activation of the pituitary adenylate cyclase-activating polypeptide and its receptor PAC1 are involved in the stress axis and the development of stress-related disorders. We recently developed PA-915, a small-molecule, non-peptide, high-affinity PAC1 antagonist, and demonstrated that it significantly suppresses anxiety-like behavior in acute stress-induced mice.

Decrease and recovery of integrated information Φ during anesthesia and sleep on human functional magnetic resonance imaging.

Integrated information theory (IIT) offers an axiomatic framework based on phenomenological properties, allowing the quantification and characterization of consciousness through a measure known as Φ. According to IIT, Φ reflects the level of consciousness and is expected to decrease with loss of consciousness, although empirical data supporting this claim remain limited. In this study, we analyzed two functional magnetic resonance imaging (fMRI) datasets acquired during anesthesia (propofol-induced) and natural sleep to determine whether Φ changes with the loss and recovery of consciousness.

Axillary Dissection with Lobectomy and Chest Wall Resection for Locally Advanced Primary Lung Cancer.

Introduction: Lung cancer with chest wall (CW) involvement can develop metastases directly to the ipsilateral axillary lymph node (ALN) via lymphatic flow of the CW. Such metastatic ALNs should be evaluated as locoregional metastases, and multimodal treatment of patients with stage III lung cancer including surgery may be utilized.

Case Presentation: A 59-year-old man presented with a chief complaint of back pain and was diagnosed as having primary lung adenocarcinoma of the right upper lobe with CW involvement and an ipsilateral ALN metastasis (cT3N0M1b: IVA, 8th edition of the tumor, node, metastasis).

Effect of Preoperative Nutritional Status on Postoperative Clinical Outcomes of Patients with Metastatic Spinal Tumors: Japan Association of Spine Surgeons with Ambition Multicenter Study.

Study Design: A multicenter prospective study.

Objective: To evaluate the effect of preoperative nutritional status, assessed using the Prognostic Nutritional Index (PNI), on clinical outcomes, including survival prognosis, postoperative complications, hospitalization duration, and functional prognosis, in patients with metastatic spinal tumors undergoing surgery.

Summary Of Background Data: Malnutrition is common in patients with cancer and is associated with poor clinical outcomes.

Features of mismatch negativity in an at-risk mental state with the traits associated with the autistic spectrum.

Introduction: Accurately distinguishing individuals with autism spectrum disorder (ASD) from those with schizophrenia spectrum disorder (SSD) can be challenging, especially in individuals with an at-risk mental state (ARMS) for psychosis. Given the need for objective markers, we focused on mismatch negativity (MMN). This study aimed to determine whether ARMS individuals with ASD traits exhibit different MMN patterns compared to ARMS individuals without such traits and healthy controls.

Experimental study on improving the durability of underground/underwater structures by mixing impregnating agents into unhardened concrete.

Components exposed to soil and water are difficult to maintain during their service life, and have traditionally been designed to ensure greater durability than components exposed to air. However, in recent years, severe damage caused by aging has also been observed in components exposed to soil and water, prompting the development of methods to achieve higher durability. To address this issue, the authors developed a method (mixing method) that enhances the durability of concrete components by mixing sodium silicate-based impregnation materials with fresh concrete.

A novel synthetic bile acid derivative inhibits hepatitis B virus infection at entry step by interfering with the oligomerization of sodium taurocholate co-transporting polypeptide.

Hepatitis B virus (HBV) infection is a major global health burden worldwide despite the availability of an effective vaccine and effective anti-HBV drugs. The currently approved anti-HBV drugs-i.e.

[Microglia-mediated cognitive impairment induced by methamphetamine].

More than half of chronic methamphetamine (METH) users exhibit multi-domain cognitive deficits, including impaired attention, executive function, and memory. MRI studies consistently demonstrate hippocampal atrophy and frontotemporal cortical thinning; these structural changes spatially overlap with glial activation, indicating the coexistence of morphological damage and ongoing neuroinflammation. To clarify causality, we developed a mouse model in which low-dose METH is micro-infused into the nucleus accumbens.

Correction: Unveiling the role of cobalt in the product regulation for CO hydrogenation to light olefins over alumina-supported Co-Fe catalysts.

[This corrects the article DOI: 10.1039/D5SC04407C.].

Label-free diagnostic procedure for hirschsprung's disease to detect intestinal mucosal characteristics of aganglionosis by Raman spectroscopy with optimized decision algorithms.

Purpose: Hirschsprung's disease (HSCR) is an intestinal disorder characterized by the absence of nerve cells in parts of the intestinal tract. The definitive diagnosis is confirmed by a full-thickness rectal biopsy to verify the absence of ganglion cells. However, incomplete removal often causes post-operative complications.

Quantitative evaluation of crystallinity of active pharmaceutical ingredients in amorphous solid dispersion using T relaxometry measured by time-domain NMR.

The aim of this study is to propose a novel method for quantifying the crystallinity of active pharmaceutical ingredients (APIs) in solid dispersion (SD)-based pharmaceuticals. The key technology employed for this quantitative method is time-domain NMR (TD-NMR) T relaxometry. Model SDs were prepared using indomethacin (IMC) and polyvinylpyrrolidone (PVP).

A novel method for generating chimeric antigen receptor-transduced primary human natural killer cells by the use of multiple cytokines and anti-CD2 and anti-NKp46 stimulatory antibodies.

Allogenic chimeric antigen receptor (CAR)-transduced natural killer (NK) cells have garnered attention due to their efficacy, safety, and potential off-the-shelf nature in patients with refractory and relapsed hematological malignancies. However, producing clinical doses of genetically modified NK cells remains challenging. The current methods for ex vivo expansion and genetic modification of primary NK cells depend on irradiated feeder cells, particularly K562 leukemic cells enforced to express stimulatory molecules.

Molecular basis of potent antiviral HLA-C-restricted CD8 T cell response to an immunodominant SARS-CoV-2 nucleocapsid epitope.

The emergence of SARS-CoV-2 Variants of Concern (VOC) is a major clinical threat; however, VOC remain susceptible to cytotoxic T lymphocyte (CTL) recognition. Therefore, it is crucial to identify potent CTL responses targeting conserved epitopes across VOCs. Here, we demonstrate that the nucleocapsid (N) protein induces efficient CTL responses in early pandemic COVID-19 convalescent donors.

Optimal Timing of Vericiguat Therapy in Patients with Heart Failure with Reduced/Mildly Reduced Ejection Fraction for Improving Mortality and Heart Failure Re-Hospitalization Rate.

: Vericiguat, a soluble guanylate cyclase stimulator, reduces cardiovascular events in patients with heart failure with reduced ejection fraction following clinical deterioration against guideline-directed medical therapy. However, the optimal timing for initiating vericiguat remains unclear. : We retrospectively analyzed heart failure patients with reduced/mild-reduced ejection fraction who received vericiguat between 2021 and 2025 upon optimal guideline-directed medical therapy.

Interplay of Oxidative Stress, Autophagy, and Rubicon in Ovarian Follicle Dynamics: Orchestrating Ovarian Aging.

Organ functions generally decline with age, but the ovary is a prototypical organ that undergoes functional loss over time. Autophagy plays a crucial role in maintaining organ homeostasis, and age-related upregulation of the autophagy inhibitor protein, Rubicon, has been linked to cellular and tissue dysfunction. This review describes how granulosa cell autophagy supports follicular growth and oocyte selection and maturation by regulating cellular energy metabolism and protein quality control.

Relationship Between Postoperative Feeding Status and Salivary Bacterial Load Following Oral Surgical Procedures.

Introduction:  Oral and maxillofacial surgery involves clean-contaminated wounds exposed to saliva, increasing the risk of surgical site infections (SSIs). While systemic antibiotics are commonly used postoperatively, the effect of feeding status on salivary bacterial counts remains unclear.

Methods:  This prospective observational study included 24 patients (greater than or equal to six years) undergoing oral surgery with postoperative enteral feeding.

Automated Assessment to Predict Lethal Arrhythmias in Brugada Syndrome: Significance of R' in Lead III.

Background: Previous investigations of predictors of sudden cardiac arrest (SCA) in Brugada syndrome (BrS) have been conducted using manual electrocardiogram (ECG) measurement, which lacks objectivity. This study aimed to examine predictive factors for SCA in BrS using automated ECG measurements.

Methods: In 270 patients with BrS, ECGs were recorded using the same electrocardiograph when a Type 1 ECG was initially observed.

Dynamics of color vision recovery in Vogt-Koyanagi-Harada disease: a longitudinal study using cone contrast test and adaptive optics imaging.

Background: Vogt-Koyanagi-Harada (VKH) disease affects visual function, but the recovery process of color vision remains incompletely understood. This study aimed to assess color vision recovery in VKH using cone contrast testing and explore its relationship with cone cell density measured using adaptive optics imaging.

Methods: Twenty-two eyes of 11 patients with VKH were evaluated at baseline (serous retinal detachment resolution) and at 3, 6, and 12 months post-treatment.