Label-free diagnostic procedure for hirschsprung's disease to detect intestinal mucosal characteristics of aganglionosis by Raman spectroscopy with optimized decision algorithms.

Purpose: Hirschsprung's disease (HSCR) is an intestinal disorder characterized by the absence of nerve cells in parts of the intestinal tract. The definitive diagnosis is confirmed by a full-thickness rectal biopsy to verify the absence of ganglion cells. However, incomplete removal often causes post-operative complications.