Interobserver Agreement in Immunohistochemical Evaluation of Folate Receptor Alpha (FRα) in Ovarian Cancer: A Multicentre Study.

Folate receptor alpha (FRα) is a high-affinity folate transporter overexpressed in various epithelial malignancies, particularly high-grade serous ovarian carcinoma. Given its restricted expression in normal tissues and accessibility in tumors, FRα is an emerging therapeutic target. Immunohistochemistry (IHC) is the standard method for FRα assessment; however, interpretation is semi-quantitative and prone to interobserver variability.

Ocular and Neurological Sequelae in Long COVID: Dry Eye, Asthenopia, Sleep Disorders, Asthenia, and Restless Legs Syndrome-A Case Report with Literature Review.

This case report presents a unique constellation of symptoms-including dry eye disease, visual and general asthenia, sleep disturbances, and restless legs syndrome-in a patient with a recent history of coronavirus disease 2019 (COVID-19) infection. While these symptoms have individually been associated with either COVID-19 or long COVID, their concurrent presentation and the simultaneous, positive response across all manifestations to a combined therapeutic regimen have not been previously described in a single case. The patient demonstrated notable improvement in both ocular and systemic symptoms following a six-week treatment with topical tear substitutes and oral administration of melatonin, and a multivitamin supplement including B-complex vitamins, antioxidants, and neuroprotective agents (Colinplus Delta, Farmaplus Italia Srl, Via Giovanni Porzio 4, 80143 Napoli, Italy).

Personal and General Views on Aging, Non-Communicable Diseases, and Their Interaction as Cross-Sectional Correlates of Vigorous Physical Activity in UK Individuals Aged 50.

: This study investigated the cross-sectional associations of personal and general views on aging, number of non-communicable diseases, and their interactions as cross-sectional predictors of vigorous physical activity. : Participants were 1699 individuals aged 50 years and over (Mean age = 67.79) and living in the community in the UK; 70.

The Impact of a Video-Educational and Tele-Supporting Program on the Caregiver-Stroke Survivor Dyad During Transitional Care (D-STEPS: Dyadic Support Through Tele-Health and Educational Programs in Stroke Care): A Longitudinal Study Protocol.

: Stroke is a leading cause of long-term disability and substantially affects the quality of life (QoL) of both survivors and their caregivers. The transition from hospital to home is a vulnerable period characterized by discontinuity of care and insufficient caregiver support. Dyadic interventions-targeting both the survivor and caregiver-have shown promise in improving recovery outcomes.

PCOS and the Genome: Is the Genetic Puzzle Still Worth Solving?

Polycystic ovary syndrome (PCOS) is a complex and multifactorial disorder affecting reproductive, endocrine, and metabolic functions in women of reproductive age. While environmental and lifestyle factors play a role, increasing evidence highlights the contribution of genetic and epigenetic mechanisms to its pathogenesis. This narrative review aims to provide an updated overview of the current evidence regarding the role of genetic variants, gene expression patterns, and epigenetic modifications in the etiopathogenesis of PCOS, with a focus on their impact on ovarian function, fertility, and systemic alterations.

New Neonatal and Prenatal Approach to Home Therapy with Amoxicillin, Rifaximin, and Anti-Inflammatory Drugs for Pregnant Women with COVID-19 Infections-Monitoring of Fetal Growth as a Prognostic Factor: A Triple Case Series (N.A.T.H.A.N.).

Since the COVID-19 pandemic, managing acute infections in symptomatic individuals, regardless of vaccination status, has been widely debated and extensively studied. Even more concerning, however, is the impact of COVID-19 on pregnant women-especially its effects on fetuses and newborns. Several studies have documented complications in both expectant mothers and their infants following infection.

Comparative Effects of Pulsed Field and Radiofrequency Ablation on Blood Cell Parameters During Pulmonary Vein Isolation.

Pulsed field ablation (PFA) is a novel non-thermal modality for pulmonary vein isolation (PVI) in atrial fibrillation (AF), offering myocardial selectivity through irreversible electroporation while sparing surrounding structures. However, concerns have emerged regarding potential subclinical hemolysis, reflected by alterations in biochemical markers such as lactate dehydrogenase (LDH). We conducted a retrospective, single-center study involving 249 patients undergoing PVI: 121 treated with PFA (PulseSelect or FARAPULSE) and 128 with radiofrequency (RF) ablation (PVAC catheter).

Muscle Wasting and Treatment of Dyslipidemia in COPD: Implications for Patient Management.

Chronic Obstructive Pulmonary Disease (COPD) is a multifactorial condition associated with significant systemic complications such as cardiovascular disease (CVD), metabolic disorders, muscle wasting, and sarcopenia. While Body Mass Index (BMI) is a well-established indicator of obesity and has prognostic value in COPD, its role in predicting disease outcomes is complex. Muscle wasting is prevalent in COPD patients and exacerbates disease severity, contributing to poor physical performance, reduced quality of life, and increased mortality.

Antioxidant Activity of Apple By-Products at Different Ripening Stages: A Sustainable Valorization Approach.

This study explores the sustainable valorization of apple by-products by examining the polyphenolic content and antioxidant activity of peel, flesh, and core at two ripening stages. Ripening significantly enhanced the concentration of bioactive compounds, particularly in the peel, where total polyphenols increased from 124.4 to 423.

Oxidative Stress and Poly(ADPribosyl)ation in Zebrafish Eyes After Exposure to Aluminium.

Aluminium (Al) is a widespread environmental contaminant known to induce oxidative stress and genotoxic effects in aquatic organisms. While its neurotoxic properties are well documented, the molecular impact of Al on the visual system remains poorly understood. In this study, adult zebrafish () were exposed to 11 mg/L Al for 10, 15, and 20 days to investigate the oxidative and genotoxic responses in ocular tissue.

Predicting Cognitive Decline in Parkinson's Disease Using Artificial Neural Networks: An Explainable AI Approach.

Background/objectives: The study aims to identify key cognitive and non-cognitive variables (e.g., clinical, neuroimaging, and genetic data) predicting cognitive decline in Parkinson's disease (PD) patients using machine learning applied to a sample ( = 618) from the Parkinson's Progression Markers Initiative database.

Multimodal imaging in young male with bicuspid aortic valve, right-sided aorto-atrial fistula and single coronary artery.

A 35-year-old male, without significant cardiovascular history, presented with recurrent palpitations. Initial echocardiographic evaluation demonstrated eccentric left ventricular hypertrophy, mild systolic dysfunction, suspicion of a ventricular septal defect, bicuspid aortic valve, and right ventricular dilation. Transesophageal echocardiography revealed an aneurysmal dilation of the right coronary sinus with an aorto-atrial/ventricular fistula, further confirmed by contrast-enhanced computed tomography angiography.

Effects of Trichoderma treatments on the phenolic and sensory quality of Aglianico grapes and wine.

Background: As environmental awareness grows, interest in sustainable agriculture is increasing. A promising alternative is the use of plant-beneficial microorganisms such as Trichoderma spp., which suppress pathogens, promote growth and enhance productivity.

Intravesical recurrence after therapeutic ureteroscopy for upper tract urothelial carcinoma: a meta-analysis.

Objective: To assess the role of ureteroscopy (URS) with laser ablation on oncological outcomes, namely, intravesical recurrence (IVR) rate, radical nephroureterectomy (RNU)-to-treat ratio and overall survival (OS), in patients with upper tract urothelial carcinoma (UTUC), an area of study that has been insufficiently addressed in the literature.

Materials And Methods: A systematic search (PROSPERO: CRD42025642480) was performed according to the Preferred Reporting Items for Systematic Reviews and Meta-analyses (PRISMA) statement. A pooled analysis was performed to quantify the effect size (ES) for IVR rate, radical nephroureterectomy (RNU)-to-treat ratio and OS.

Engineering gold nanoparticles aggregation in acrylate hydrogel-photopolymers for SERS-based on-site highly sensitive dimethoate detection on olives.

Hydrogel-based flexible plasmonic devices represent a cutting-edge technology for real-time monitoring of food safety, particularly for pesticide detection. This study presents a cost-effective, portable, and sensitive method to detect dimethoate (DMT), a hazardous organophosphorus pesticide, at concentrations below the maximum residue limit (MRL) of 0.01 ppm on olives.

Association between bioelectrical impedance analysis parameters and muscle-related measurements obtained using a non-diagnostic ultrasound-based technique.

Background: An in-depth analysis of body composition (BC) is fundamental for the assessment of nutritional status. A multifactorial approach is important for this assessment, integrating parameters and measurements obtained through different techniques, most notably bioelectrical impedance analysis (BIA) and ultrasound. In this study, we aimed to evaluate associations between BIA parameters and ultrasound measurements.

Polystyrene Microplastic Interferes with Yolk Reserve Utilisation in Early Nauplii.

Polystyrene microfragments are among the most common plastic pollutants globally. They significantly affect aquatic life, harming various organs and tissues. In this study, we examined the effects of 3 µm polystyrene beads (MPs, 20 µg/L) on development and yolk resorption in pre-feeding nauplii of , a lecithotrophic crustacean used in toxicity testing.

Consider the unexpected! An overlooked, elusive, rare but dramatic diagnosis: anorectal melanoma.

Background: Anorectal melanoma (AM) is a rare and aggressive malignancy, often misdiagnosed due to its clinical resemblance to benign anorectal conditions. Early diagnosis remains challenging, with a poor prognosis and high rates of metastasis at presentation.

Methods: We conducted a retrospective multicenter study of 21 patients diagnosed with AM between 2013 and 2023 across four high-volume Italian surgical centers.

TSLP: contrasting roles in cancer.

Thymic stromal lymphopoietin (TSLP) is an alarmin cytokine possessing a plethora of pleiotropic properties. Human and mouse TSLP exerts their activity a heterodimeric complex composed of TSLP receptor (TSLPR) chain and IL-7Rα. TSLP is predominantly expressed by epithelial cells and keratinocytes but can also be produced by several immune cells and some cancers.

Reduced rank regression for mixed predictor and response variables.

In this paper, we propose the generalized mixed reduced rank regression method, GMR for short. GMR is a regression method for a mix of numeric, binary and ordinal response variables. The predictor variables can be a mix of binary, nominal, ordinal and numeric variables.

Optimising implantable loop recorder alerts with artificial intelligence: role of R-wave amplitude and workload reduction.

Background: False-positive (FP) alerts from implantable loop recorders increase clinical workload and may delay appropriate intervention. AccuRhythm AI, a cloud-based filtering algorithm, is designed to reduce these alerts in Reveal LINQ and LINQ II devices. This study assessed the algorithm's effect on FP and clinician burden reduction, with a focus on the influence of R-wave sensing amplitude.

Investigating temperature-induced unfolding pathways of DNA G-quadruplexes via 2D UV resonant Raman spectroscopy.

G-quadruplexes are highly polymorphic noncanonical secondary structures of nucleic acids that play important biological roles. Understanding their unfolding mechanisms is essential for elucidating their stability and interactions. In this study, we investigated the thermal unfolding pathways of four biologically relevant G-quadruplex-forming DNA sequences using ultraviolet resonance Raman (UVRR) spectroscopy combined with advanced spectral analysis techniques.

Marine-Inspired Ovothiol Analogs Inhibit Membrane-Bound Gamma-Glutamyl-Transpeptidase and Modulate Reactive Oxygen Species and Glutathione Levels in Human Leukemic Cells.

The enzyme γ-glutamyl transpeptidase (GGT), located on the surface of cellular membranes, hydrolyzes extracellular glutathione (GSH) to guarantee the recycling of cysteine and maintain intracellular redox homeostasis. High expression levels of GGT on tumor cells are associated with increased cell proliferation and resistance against chemotherapy. Therefore, GGT inhibitors have potential as adjuvants in treating GGT-positive tumors; however, most have been abandoned during clinical trials due to toxicity.