Treatment Strategies for Patients with Mitral Regurgitation: A Meta-Analysis of Randomized Controlled Trials.

: Several treatment strategies are available for patients with mitral valve regurgitation (MR). However, evidence regarding their comparative effectiveness remains limited. We sought to compare the performance of different treatment strategies for personalized treatment of patients with MR.

Delayed vs. Concomitant Urethrectomy for Non-Metastatic Urothelial Carcinoma of the Urinary Bladder Undergoing Radical Cystectomy: Perioperative and Survival Outcomes from a Single Tertiary Centre in the United Kingdom.

The role of urethrectomy at the time of Robotic-Assisted or Open Radical Cystectomy (RARC, ORC) is controversial. Whether urethrectomy should be performed at the time of RARC/ORC or delayed up to a 3-6 month interval is unclear. We performed a retrospective cohort analysis of perioperative and survival outcomes in patients with high-risk NMIBCs or non-metastatic MIBCs at our institution who underwent either concomitant or deferred urethrectomy after RC.

Functional Mitral Regurgitation in the Transcatheter Era: Diagnostic and Therapeutic Pathways.

Functional mitral regurgitation (FMR) is a common condition with significant prognostic implications, primarily driven by left atrial or ventricular remodeling secondary to ischemic or non-ischemic cardiomyopathies. While guideline-directed medical therapy (GDMT) remains the cornerstone of management, reducing mitral regurgitation severity in up to 40-45% of cases, additional interventions are often necessary. In patients where atrial fibrillation (AF) or ventricular dyssynchrony due to abnormal electrical conduction contributes to disease progression, guideline-directed AF management or cardiac resynchronization therapy plays a pivotal role.

Development and Validation of LC-MS/MS and IC-HRMS Methods for Highly Polar Pesticide Detection in Honeybees: A Multicenter Study for the Determination of Pesticides in Honeybees to Support Pollinators and Environmental Protection.

The widespread use of agrochemicals raises concerns about environmental impacts, particularly on pollinators, such as bees, which serve as bioindicators of contamination. Developing methods to assess contamination risks in bioindicators supports regulatory frameworks, including EU regulations on the maximum residue limits (MRLs) for pesticides in food and the environment. This study presents the development and validation of two complementary analytical methods (LC-MS/MS and IC-HRMS) for highly polar pesticide (HPP) detection and quantification in bee matrices.

Evaluation of Internal and Marginal Shrinkage Stress in Adhesive Class III Cavities Restored with Different Resin Composite Combinations-A 3D-FEA Study.

To study the effects of internal and marginal polymerization shrinkage stress and distribution in different resin composite class III dental restorations in relation to the restorative technique using numerical finite element analysis (FEA). A 3D model of a human hemi-maxilla with a sound maxillary central incisor were created. Four class III distal cavities were shaped and differently restored.

The 2018 Classification of Periodontitis: Challenges from Clinical Perspective.

: The objective of this narrative review was to evaluate the clinical challenges encountered in the application of the 2018 AAP/EFP Classification of Periodontitis. : Electronic and manual searches were conducted to identify studies reporting diagnostic accuracy and inter- and intra-examiner agreement when using the 2018 Classification, both with and without the aid of implementation tools. : Eleven studies were included, encompassing a total of 459 clinical cases.

Heterozygous variants in affect hearing, vision, cardiac, and immune function.

Phospholipase C isozymes (PLCs) hydrolyze phosphatidylinositol 4,5-bisphosphate (PIP) into inositol 1,4,5-trisphosphate (IP) and diacylglycerol (DAG), important signaling molecules involved in many cellular processes including Ca release from the endoplasmic reticulum (ER). encodes the PLCγ1 isozyme that is broadly expressed. Hyperactive somatic mutations of are observed in multiple cancers, but only one germline variant has been reported.

Microbiome profiling of Grana Padano and Parmigiano Reggiano cheeses reveals cheese-specific biomarkers, psychobiotic potential, and bioprotective activities.

Grana Padano (GP), Trentingrana (TG), and Parmigiano Reggiano (PR) are among the finest Italian Protected Designation of Origin (PDO) cheeses. GP, TG, and PR undergo extensive proteolysis during ripening, where the microbiome metabolizes amino acids, producing flavour and bioactive molecules. We explored the microbiome, volatilome, and metaproteome of PDO GP (n = 42), TG (n = 18), and PR (n = 60).

Adverse cardiovascular events in coronary Plaques not undeRgoing pErcutaneous coronary intervention evaluateD with optIcal Coherence Tomography. The PREDICT-AI risk model.

Introduction: Most acute coronary syndromes (ACS) originate from coronary plaques that are angiographically mild and not flow limiting. These lesions, often characterised by thin-cap fibroatheroma, large lipid cores and macrophage infiltration, are termed 'vulnerable plaques' and are associated with a heightened risk of future major adverse cardiovascular events (MACE). However, current imaging modalities lack robust predictive power, and treatment strategies for such plaques remain controversial.

Leukemia inhibitory factor promotes human cholangiopathies, and its inhibition improves cholestasis in Abcb4-/- mice.

Background: Primary sclerosing cholangitis (PSC) and primary biliary cholangitis (PBC) are immune-mediated cholestatic disorders characterized by progressive biliary inflammation and fibrosis, for which treatment options remain limited, underscoring the need for novel therapeutic targets. The leukemia inhibitory factor (LIF) is an IL-6-related cytokine that dysregulates the communication between epithelial cells and extracellular matrices by binding a heterodimeric complex formed by LIF receptor (LIFR) and gp130. The role of the LIF/LIFR system in PSC and PBC and its potential as a therapeutic target remain unclear.

The Clinical Significance of Atrial Fibrillation in Non-High-Risk Brugada Syndrome: The BruFib Study.

Background: Atrial fibrillation (AF) occurs in up to 20% of patients with Brugada syndrome (BrS), yet its risk factors and prognostic implications remain uncertain.

Objectives: This study sought to identify risk factors for AF in patients with non-high-risk BrS and to evaluate the impact of AF on ventricular arrhythmias (VAs), sick sinus syndrome (SSS), and stroke in non-high-risk BrS.

Methods: This was a multicenter, retrospective study conducted across 20 international centers.

The identification of adenylyl cyclase modulators as potential receptors for 6-nitrodopamine in human-induced pluripotent stem cell (hiPSC)-derived cardiomyocytes and their relevance in heart inotropism.

6-Nitrodopamine (6-ND) has potent positive chronotropic and inotropic effects. At a very low dose, i.e.

Calcium, phosphate and fluoride ionic release from dental restorative materials for elderly population: an analysis.

Introduction: With the increasing prevalence of cervical and root lesions in elderly patients, dental filling materials able to release bioactive ions are gaining importance in the restorative treatment. This study evaluated the release of calcium (Ca ), phosphate (PO ), and fluoride (F) ions from different restorative materials (, and ) for elderly population through testing conducted under different pH levels and temperature conditions.

Methods: Specimens (10 mm diameter and 2 mm thickness;  = 3 for each material) were prepared according to manufacturers' instructions, immersed in buffer solutions at pH 4.

Integrated , microarray, and network pharmacology analysis reveals the multi-target anti-diabetic potential of .

Introduction: Diabetes mellitus (DM), particularly type 2 DM (T2DM), is a chronic metabolic disorder requiring novel therapeutic approaches as the available therapies are not meeting the current challenges. This study investigates the anti-diabetic potential of Vigna unguiculata using a network pharmacology approach, supported by and analyses.

Methods: The plant was collected from Khyber Pakhtunkhwa, Pakistan, and subjected to hydroalcoholic extraction and fractionation.

Phenotypic and genotypic characterization of carbapenem encoding genes among carbapenem-resistant Gram-negative bacteria isolated from North Casablanca, Morocco.

Carbapenem resistance genes in Gram-negative bacteria (CR-GNB) are a major cause of critical infections and are considered an urgent public health concern. The present study aimed to describe the prevalence of CR-GNB and the dissemination of extended-spectrum beta-lactamase (ESBL) and carbapenemase genes in clinical isolates from Casablanca, Morocco. Firstly, the strains were collected and identified using phenotypic and biochemical methods, then the antibiotic susceptibility was evaluated by the disc diffusion assay to screen isolates resistant to carbapenems.

Advanced integrated strategy for structural and mineralogical exploration of inaccessible regions employing remote sensing and multiscale analysis of aeromagnetic data.

Rugged terrains and remote desert environments present notable challenges for geological data analyses due to limited accessibility and scarcity of surface and subsurface data. One of such challenging areas is Wadi Dif, located in South Eastern Desert (SED) of Egypt. This study presents an integrated approach combining aeromagnetic and remote sensing data to effectively investigate such environments.

Leveraging agent-based models and deep reinforcement learning to predict taxis in cell migration.

We present a novel computational framework that combines Agent-Based Modeling (ABM) with Reinforcement Learning (RL) using the Double Deep Q-Network (DDQN) algorithm to determine cellular behavior in response to environmental signals. With this approach, the model captures the transduction of environmental cues into biological responses directly from experimental observations, without explicitly predefining cell behavior. This enables the prediction of dynamic, environment-dependent cell behavior and offers a scalable and flexible alternative to traditional rule-based ABM.

Censoring in trials testing immunotherapy in advanced cancers. A systematic review and a meta-research study.

Background: Informative censoring affects interpretation of trials results. We investigated censoring rates in randomized controlled trials (RCTs) of immune checkpoint inhibitors (ICIs).

Methods: We searched articles of RCTs testing ICIs in advanced cancers, published up to 12/2023.

ESR Essentials: artificial intelligence in breast imaging-practice recommendations by the European Society of Breast Imaging.

Artificial intelligence (AI) can enhance the diagnostic performance of breast cancer imaging and improve workflow optimization, potentially mitigating excessive radiologist workload and suboptimal diagnostic accuracy. AI can also boost imaging capabilities through individual risk prediction, molecular subtyping, and neoadjuvant therapy response predictions. Evidence demonstrates AI's potential across multiple modalities.

Impact of hyaluronic acid molecular weight on silibinin intranasal delivery via hybrid nanoparticle formulation.

This study aimed to develop hybrid nanoparticles (LPNs) using hyaluronic acid (HA) of different molecular weights for intranasal delivery of silibinin (SLB) to glioma tumors. LPNs were prepared using low, medium, and high molecular weight HA. Blank LPNs measured approximately 100 nm, whereas SLB-LPNs were about 160 nm.

Syncope is associated with multidimensional frailty in older adults.

Background: Multidimensional frailty and syncope are common in older adults, yet their relationship remains unclear.

Methods: This retrospective multicenter study, involving outpatients who underwent Comprehensive Geriatric Assessment, aims to evaluate the relationship between frailty and syncope. Patients were classified as fit/light frail, moderate frail or severe frail based on their fr-AGILE score.