Enhancing the nutritional profile of purple sweet potato flour through fermentation with InaCC B157: A functional food perspective.

Background And Aim: Purple sweet potatoes ( var. Ayamurasaki) possess high nutritional potential due to their rich content of amino acids, minerals, and fatty acids. However, their nutritional profile can be further improved through fermentation.

Evaluation of Roxb. extract as a functional alternative to antibiotic growth promoters in broiler chicken nutrition.

Background And Aim: The global ban on antibiotic growth promoters (AGPs) in poultry production has intensified the search for effective phytogenic alternatives. Roxb., commonly known as Javanese turmeric, exhibits antimicrobial and antioxidant properties attributed to its bioactive compounds, including xanthorrhizol and curcumin.

Forest Type, Bark Wounding, and Tapping: Their Combined Influence on Bacteria Biota of Styrax Paralleloneurus in Natural and Community Forest.

Styrax paralleloneurus is a resin-producing tree native to Sumatra, Indonesia. This study investigated the effects of tapping, bark wounding and forest type on bacterial biota in the stem of styrax in natural and community forests. Amplicon metagenomic sequencing of the 16S rRNA region was deployed to identify the bacterial communities associated with tapped and untapped trees across various environmental and experimental conditions.

High-efficiency chemiluminescent POCT assay for anti-Müllerian hormone: innovative development and application in polycystic ovary syndrome diagnosis.

In recent years, the decline in ovarian reserve function has become a major cause of reduced female fertility. Early identification, evaluation, and management of ovarian reserve dysfunction are essential for improving female fertility and reproductive health. Currently, the main diagnostic method is assessing ovarian function through measuring AMH serum concentration and follicle count.

Efficacy of MAA01-influenced hydroxyapatite/graphene oxide nanocomposites for bone ossification.

This study investigates hydroxyapatite (HAp) nanocomposites infused with MAA01-pretreated graphene oxide (GO) for bone tissue engineering. Hydrothermally synthesized HAp/GO (HH-G) nanocomposites showed rod with sheetlike structures with nano level crystallite size, and appropriate bioactivity and degradability. Raman spectroscopy confirmed the successful reduction of GO to reduced graphene oxide (rGO), enhancing antibacterial properties and cellular interactions.

Methylene blue removal using alginate-PVA- biocomposite: Kinetics and biodegradation studies.

Methylene blue (MB) is a common synthetic dye used in various industries due to its abundance and cost-effectiveness. However, the presence of this synthetic dye in industrial wastewater has the potential to cause significant effects on the environment and public health when released into soil or water bodies. According to results, biological wastewater treatment has emerged as a promising approach due to its economic feasibility, efficiency, and environmental compatibility.

Direct Detection of Textile Dyes in Environmental Samples by LDI and MALDI FT-ICR MS.

Dye contamination poses a serious problem in some developing countries, which often lack the facilities for effective analysis. International shipment of samples for high-resolution mass spectrometry is hindered by strict regulations and high costs. Therefore, there is a need for a direct, low-volume analytical method.

Formation of an Amyloid-like Structure During In Vitro Interaction of Titin and Myosin-Binding Protein C.

Protein association and aggregation are fundamental processes that play critical roles in a variety of biological phenomena from cell signaling to the development of incurable diseases, including amyloidoses. Understanding the basic biophysical principles governing protein aggregation processes is of crucial importance for developing treatment strategies for diseases associated with protein aggregation, including sarcopenia, as well as for the treatment of pathological processes associated with the disruption of functional protein complexes. This work, using a set of methods such as atomic force microscopy (AFM), transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction, as well as bioinformatics analysis, investigated the structures of complexes formed by titin and myosin-binding protein C (MyBP-C).

Hydrothermal synthesis of nanohydroxyapatite-activated carbon composites and its slow-release performance for urea.

Urea is the most widely used nitrogen-based fertilizer, but its excessive application leads to nutrient loss through leaching and evaporation. This study presents a nanohydroxyapatite (nHA)-activated carbon (AC) composite synthesized via hydrothermal method as a slow-release material (SRM) for urea. The composites of nHA-AC (HC) enhances urea adsorption and release control.

Study of the Bactericidal Efficacy of Short-Wave UV Irradiation on Pathogenic Klebsiella pneumoniae, Moraxella catarrhalis, and Kocuria rhizophila by Confocal Laser Scanning Microscopy.

The effect of UV radiation in the range of 185-254 nm on the viability of stress-resistant dormant forms of Moraxella catarrhalis, Kocuria rhizophila and biofilm-forming Klebsiella pneumoniae was studied. This bacterial form is much more resistant to UV irradiation in comparison with vegetative (planktonic) cultures. The antimicrobial efficacy of the experimental UV emitter (Ishlinsky Institute for Problems in Mechanics, Russian Academy of Sciences) was studied by confocal microscopy with differentiated staining of live/dead cells of K pneumoniae, M.

Optimization and Partial Characterization of Trypsin-Like Protease Enzyme (TLP) from Indonesian Indigenous Lactiplantibacillus plantarum M4 for Digestive Enzyme.

Trypsin is a digestive enzyme crucial for protein breakdown and the activation of other digestive enzymes. Several studies have shown that trypsin deficiency often leads to various health problems, indicating the need to produce its supplements. These supplements can be obtained from various sources, including different strains of Lactiplantibacillus plantarum, which produces a protease similar to trypsin, called trypsin-like protease (TLP).

Combinatorial Effects of CPP-Modified Antimicrobial Peptides: Synergistic and Additive Interactions Against Pathogenic Bacteria.

The development of novel antimicrobial peptides (AMPs) with broad-spectrum activity represents a promising strategy to overcome multidrug resistance in pathogenic bacteria. In this study, we investigated the antimicrobial activity of three designed peptides-R44K*, V31K*, and R23F*-engineered to incorporate an amyloidogenic fragment from the S1 protein of and one or two cell-penetrating peptide (CPP) fragments to enhance cellular uptake. The antimicrobial efficacy of these peptides and their combinations was assessed against , , , methicillin-resistant (MRSA), and .

Carbapenem-resistant Acinetobacter baumannii isolate BAL114 from Vietnam produces capsular polysaccharide with two forms of the K58 unit.

Capsular polysaccharide (CPS) produced by two Acinetobacter baumannii isolates carrying the KL58 locus were recently determined and found to differ due to the presence of additional CPS biosynthesis genes found in prophage. Here, we have examined the CPS produced by a third KL58-carrying A. baumannii isolate, BAL114, which was recovered in 2009 in Vietnam.

Production of recombinant D-allulose 3-epimerase utilizing an auto-induction approach in fermentor cultures suitable for industrial application.

D-Allulose 3-epimerase (DAEase) is the key enzyme catalyzing D-fructose to catalyze into D-allulose, a rare sugar in foods, which has lately drawn increasing worldwide attention owing to its possible health advantages and application as a substitute sucrose. This work focused on the development of an economical, scalable production method of DAEase by using the Escherichia coli BL21 star™ (DE3) as host expression. The research work aims to optimize the production of the enzyme through an auto-induction strategy in chemically defined media by using lactose as a natural inducer, thereby overcoming various limitations of conventional IPTG induction methods.

Genome assembly of strain 1432 carrying KL130 and eight prophage regions.

We report the genome assembly of strain 1432, a bacterial host for the previously described phage vB_AbaP_AS12, which was isolated from a burn patient in Nizhny Novgorod, Russia. The strain was found to carry capsule biosynthesis locus KL130. Eight prophage regions were bioinformatically predicted in the genome assembly.

Enhancement of Recombinant Adeno-Associated Virus Production in HEK293 Cells by Application of Small Molecule Additives and Genetic Regulation Based on Perfusion Technique.

Adeno-associated virus (AAV) vectors offer numerous advantages, including low immunogenicity and a high safety profile, but their development and wide application are still hindered by some technical limitations. Recent studies have shown that small molecule chemical additives can significantly increase the yield of rAAV vectors in HEK293. Our study found that the antimitotic nocodazole, a positive regulator of the rAAV genomic titer, approximately doubled the yield of rAAV vectors.

DC-SIGN (CD209)-mediated interactions between bacteria, lung cancer tissues, and macrophages promote cancer metastasis.

One of the hallmarks of lung cancers is the earlier metastasis resulting from the dissemination of cancer cells. Although accumulating evidence suggests that bacterial infection may be involved in the development of the metastasis of lung cancer, few studies have explored the molecular mechanisms of bacterial infection in the dissemination of lung cancer cells. A series of studies have indicated that certain Gram-negative bacteria are able to hijack antigen-presenting cells (APCs) via interaction with DC-SIGN (CD209) receptors to facilitate the dissemination of pathogens, including viruses, bacteria, fungi, and parasites.

Optimization of ultrasound-assisted deep eutectic solvent extraction, characterization, and bioactivities of flavonoids from Cercis glabra leaves.

This study presents an innovative and environmentally friendly approach for extracting flavonoids from Cercis glabra leaves using ultrasound - assisted deep eutectic solvent extraction (UAE - DESs). Through systematic evaluation of 22 deep eutectic solvent (DESs) formulations, choline chloride-levulinic acid (ChCl:Lev) was identified as the optimal solvent system. Response surface methodology optimization revealed ideal extraction parameters: a liquid - solid ratio of 25:1 mL/g, extraction temperature of 40 °C, time of 39 min, achieving a flavonoid yield of 8.

Complexity of antibiotic resistance and its impact on gut microbiota dynamics.

The present review explores the influence of the gut microbiota on antibiotic resistance dynamics, particularly those associated with dysbiosis. The improper use of antibiotics can induce resistance in pathogens through various pathways, which is a topic of increasing interest within the scientific community. This review highlights the importance of microbial diversity, gut metabolism, and inflammatory responses against the dysbiosis due to the action of antibiotics.

Biophysical and microbiological aspects of the interaction between Yersinia pestis PsaA and bacteriophage L-413C.

There has been a great interest in developing the phage-containing remedy against plague caused by antimicrobial resistant strains of Yersinia pestis, which have been increasingly isolated in recent years from sick humans and animals. Studies thus are under way to develop a "phage cocktail", which is expected to be effective against a wide range of pathogenic strains. Our paper sheds light on the role of Y.

Unveiling the ecological processes driving soil and lichen microbiome assembly along an urbanization gradient.

Global biodiversity loss is accelerating due to the transformation of natural landscapes into agricultural and urban areas. Yet, research on the urbanization impact on environmental and host-associated microbiomes, particularly on the ecological processes that mediate their assembly and function, remains scarce. This study investigated the effects of an urbanization gradient on the diversity and assembly processes of the soil microbiome and the microbiomes of three epiphytic lichen species (Candelaria concolor, Physcia adscendens, and Xanthoria parietina).

Lipoic acid in metabolic dysfunction-associated steatotic liver disease: a review.

The incidence and prevalence of metabolic dysfunction-associated steatotic liver disease (MASLD), a chronic liver disease characterized by hepatic steatosis without substantial alcohol consumption, are rapidly increasing worldwide. Liver cirrhosis and cancer are relatively common in MASLD patients. Therefore, it is essential to take proactive measures in preventing its onset or initiating prompt treatment.

Differential expression of and genes in mammary HC11 cells challenged with and : Implications for mastitis resistance.

Background And Aim: Mastitis remains a major health challenge in dairy cattle, often caused by Gram-positive pathogens. Toll-like receptors (TLRs) and chemokine receptors (CXCRs) play essential roles in the innate immune response of mammary epithelial cells (MECs). However, the differential expression of these genes in response to specific mastitis-causing spp.

: A Novel Species of Tropical (, , and ) Discovered in UNESCO Rinjani-Lombok Biosphere Reserve, Indonesia.

A new species of was described from Mount Rinjani National Park (MRNP), Indonesia, by combining comprehensive morphological characters and molecular data from four genes (ITS, LSU, Tef1, and RPB2). The species is characterized by having large ascomata, with irregular or not parallel pits shaped and ridges; enlarged based stipe; large and spheroidal to broadly spheroidal basidiospores, corrugated or maze-like with a few striations of spore surface. Comparisons with similar taxa were also carried out using morpho-molecular data.