Reusable magnetic alginate nanocomposite with immobilized Pseudozyma antarctica yeast cells offers the potential for bioremediation and detoxification of reactive black 5 dye.

Azo dyes in wastewater of textile, paper and pulp, cosmetics, and leather tanning industries have toxicity and mutagenicity for all living organisms. The aim of the current research was the study of free and immobilized yeast cells of Pseudozyma antarctica on the beads of magnetic alginate nanocomposite to decolorize and detoxify Reactive Black 5 (RB5) as a representative of azo dyes. This yeast decolorized a high concentration of RB5 (2500 mg/L) with 82.

Assessing the Role of Myxobacteria Isolates in the Biotransformation of Flavoring Substances.

Myxobacteria are well known for producing secondary metabolites with various potential applications, including volatile compounds that serve as flavors, although this capability is less documented. This study characterizes the morphology of six Myxococcus strains (InaCC B1497, B1482, B1498, B1483, B1484, and B1486) obtained from the Indonesian Culture Collection (InaCC) and evaluates their potential for flavor biotransformation. Morphological observations were conducted on fruiting bodies, vegetative cells, myxospores, and colony shapes using dissection and binocular microscopes.

Current Paradigms and Future Challenges in Harnessing Nanocellulose for Advanced Applications in Tissue Engineering: A Critical State-of-the-Art Review for Biomedicine.

Nanocellulose-based biomaterials are at the forefront of biomedicine, presenting innovative solutions to longstanding challenges in tissue engineering and wound repair. These advanced materials demonstrate enhanced mechanical properties and improved biocompatibility while allowing for precise tuning of drug release profiles. Recent progress in the design, fabrication, and characterization of these biomaterials underscores their transformative potential in biomedicine.

Exploring the Biodiversity and Anticancer Potential of Fungal Endophytes from Peronema canescens Jack.

Peronema canescens Jack has shown promising anticancer activities. This study set out to investigate the rich biodiversity and potential anticancer properties of fungal endophytes derived from P. canescens.

Synthesis and the impact of hydroxyapatite nanoparticles on the viability and activity of rhizobacteria.

Preserving the viability of rhizobacteria during plant application poses a significant challenge when utilizing rhizobacteria as biofertilizers, especially under adverse environmental conditions. Therefore, the selection of a suitable carrier material for rhizobacteria plays a crucial role in ensuring the sustained viability of these microorganisms. Nanomaterials, particularly nanohydroxyapatite (nHA), have garnered attention for sustaining rhizobacterial viability, high loading capacity, high biodegradability, and biocompatibility, which facilitate microbial interactions.

A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor.

The DNA polymerase derived from Thermus aquaticus is the most widely utilized among various DNA polymerases, indicating its significant economic importance. Consequently, efforts to achieve a substantial yield of Taq DNA polymerase (Taq-pol) are ongoing. The expression of recombinant protein using T7-induced promoters presents challenges in cost-effectiveness, primarily due to the reliance on traditional induction method.

Novel biocomposite of Pseudomonas aeruginosa supported by metal-organic framework UiO-66 in sodium alginate-polyvinyl alcohol matrices for methylene blue decolorization: Effect of crosslinking agents and optimization using response surface methodology.

The excessive use of synthetic dyes in textile industry is significantly causing water pollution due to their toxicity and resistance to degradation. Therefore, this study aimed to investigate the immobilization of the dye-degrading bacterium Pseudomonas aeruginosa supported by the metal-organic framework (MOF) UiO-66 in sodium alginate-polyvinyl alcohol (SA-PVA) matrices to enhance methylene blue (MB) decolorization. Different crosslinkers, such as CaCl, CaCl/boric acid (CaCl/BA), CaCl/glutaraldehyde (CaCl/GA), and CaCl/boric acid/glutaraldehyde (CaCl/BA/GA) were used to fabricate the SA/PVA@UiO-66/P.

Tat-fimbriae ("tafi"): An unusual type of haloarchaeal surface structure depending on the twin-arginine translocation pathway.

The surface structures of archaeal cells, many of which exist at high temperatures, high salinity, and non-physiological pH, are key factors for their adaptation to extreme living conditions. In the haloarchaeon , we have discovered a thin filamentous surface appendage called tat-fimbriae ("tafi"), which were identified to be composed of three protein subunits, TafA, TafC, and TafE, among which TafA is the major fimbrial subunit. Molecular genetic evidence demonstrates TafA was transported through the twin-arginine translocation pathway (Tat-pathway).

Vic9 mycobacteriophage: the first subcluster B2 phage isolated in Russia.

Mycobacteriophages are viruses that specifically infect bacteria of the Mycobacterium genus. A substantial collection of mycobacteriophages has been isolated and characterized, offering valuable insights into their diversity and evolution. This collection also holds significant potential for therapeutic applications, particularly as an alternative to antibiotics in combating drug-resistant bacterial strains.

A report on the physicochemical and antioxidant properties of three Indonesian forest honeys produced by .

Indonesia, one of the largest tropical forests, offers a diverse range of nectar sources that contribute to the unique characteristics of forest honey. This study aims to investigate physicochemical and antioxidant properties of forest honey from three distinct regions of Indonesia. Key physicochemical parameters include moisture, color, electrical conductivity (EC), total dissolved solids (TDS), total suspended solids (TSS), density, diastase number (DN), hydroxymethylfurfural (HMF), pH, total acidity, ash content, protein content, and reducing sugars.

Bacteriophage and Phage-Encoded Depolymerase Exhibit Antibacterial Activity Against K9-Type in Mouse Sepsis and Burn Skin Infection Models.

is a widely distributed nosocomial pathogen that causes various acute and chronic infections, particularly in immunocompromised patients. In this study, the activities of the K9-specific virulent phage AM24 and phage-encoded depolymerase DepAPK09 were assessed using in vivo mouse sepsis and burn skin infection models. In the mouse sepsis model, in the case of prevention or early treatment, a single K9-specific phage or recombinant depolymerase injection was able to protect 100% of the mice after parenteral infection with a lethal dose of of the K9-type, with complete eradication of the pathogen.

Immunomodulatory Effect of Phage Depolymerase Dep_kpv74 with Therapeutic Potential Against K2-Hypervirulent .

The emergence of multidrug-resistant hypervirulent (hvKp) has made it difficult to treat and control infections caused by this bacterium. Previously, the therapeutic effectiveness of phage-encoded depolymerase Dep_kpv74 in a mouse model of -induced thigh soft tissue infection was reported. In this study, the effect of Dep_kpv74 on blood parameters in mice, the proliferation and subpopulation composition of spleen lymphocytes, and the activity and stability of the enzyme at different pH and temperatures were further explored.

Live Plague Vaccine Development: Past, Present, and Future.

During the last 100 years, vaccine development has evolved from an empirical approach to one of the more rational vaccine designs where the careful selection of antigens and adjuvants is key to the desired efficacy for challenging pathogens and/or challenging populations. To improve immunogenicity while maintaining a favorable reactogenicity and safety profile, modern vaccine design must consider factors beyond the choice of target antigen alone. With new vaccine technologies currently emerging, it will be possible to custom-design vaccines for optimal efficacy in groups of people with different responses to vaccination.

Genetically Engineered Bacterial Ghosts as Vaccine Candidates Against Infection.

Bacterial ghosts (BGs), non-living empty envelopes of bacteria, are produced either through genetic engineering or chemical treatment of bacteria, retaining the shape of their parent cells. BGs are considered vaccine candidates, promising delivery systems, and vaccine adjuvants. The practical use of BGs in vaccine development for humans is limited because of concerns about the preservation of viable bacteria in BGs.

Evaluation of 16S rRNA genes sequences and genome-based analysis for identification of non-pathogenic .

16S rRNA genes sequencing has been used for routine species identification and phylogenetic studies of bacteria. However, the high sequence similarity between some species and heterogeneity within copies at the intragenomic level could be a limiting factor of discriminatory ability. In this study, we aimed to compare 16S rRNA genes sequences and genome-based analysis (core SNPs and ANI) for identification of non-pathogenic .

Effect of Soaking Treatments in Acid and Salt Solutions on Physicochemical, Structural, Thermal and Rheological Properties of Porang ( Blume) Flour.

Research Background: Porang ( Blume) contains a high amount of starch, glucomannan and Ca-oxalate. Soaking porang tuber in acid (citric acid) and salt (sodium chloride) solutions affects the Ca-oxalate content, functional, rheological and thermal properties of porang flour. The aim of this study is to thoroughly investigate the effect of soaking treatments in acid and salt solutions at different temperatures on the physicochemical, rheological and thermal properties, functional groups, molecular mass and morphology of porang flour.

Isolation and characterization of native fungal strains for the bioremediation of BTEX pollutants.

Benzene, toluene, ethylbenzene, and xylene (BTEX) exposure is known to be carcinogenic and neurotoxic chemicals to humans. This study investigates the potential of fungal native strains for the bioremediation of BTEX compounds. Fungal isolates were obtained from BTEX-enriched soil, and their ability to degrade these pollutants was evaluated.

Expanding the Variety of Pyridinium-Based Bis-QACs with Antimicrobial Properties: Investigation into Linker Structure-Activity Correlation.

For decades quaternary ammonium compounds (QACs) have served as main component of a top antiseptic and disinfectant compositions. Among them, bis-QACs are the most prominent and effective class of biocides. Although mono-QACs still dominate the antiseptic market, their activity against Gram-negative bacteria is largely inferior to bis-QACs.

Lateral Flow Immunosensing of Typhimurium Cells in Milk: Comparing Three Sequences of Interactions.

To ensure the safety of foodstuffs, widespread non-laboratory monitoring for pathogenic contaminants is in demand. A suitable technique for this purpose is lateral flow immunoassay (LFIA) which combines simplicity, rapidity, and productivity with specific immune detection. This study considered three developed formats of LFIA for Typhimurium, a priority pathogenic contaminant of milk.

Exploring Compactness and Dynamics of Apomyoglobin.

Hydrogen-deuterium exchange mass spectrometry (HDX-MS) approach has become a valuable analytical complement to traditional methods. HDX-MS allows the identification of dynamic surfaces in proteins. We have shown that the introduction of various mutations into the amino acid sequence of whale apomyoglobin (apoMb) leads to a change in the number of exchangeable hydrogen atoms, which is associated with a change in its compactness in the native-like condition.

RNA-Binding S1 Domain in Bacterial, Archaeal and Eukaryotic Proteins as One of the Evolutionary Markers of Symbiogenesis.

The RNA-binding S1 domain is a β-barrel with a highly conserved RNA-binding site on its surface. This domain is an important part of the structures of different bacterial, archaeal, and eukaryotic proteins. A distinctive feature of the S1 domain is multiple presences (structural repeats) in proteins and protein complexes.

Development of Naphthalene-Derivative Bis-QACs as Potent Antimicrobials: Unraveling Structure-Activity Relationship and Microbiological Properties.

While the pandemic is behind us, the world community faces a global threat of bacterial resistance outbreak. One of the key ways to combat the spread of multi-resistant bacteria is infection prevention and control tactics using modern antiseptic and disinfectant compositions. Herein, we continue the path to unravel the structure-activity relationship (SAR) of potent pyridine-derived biocide class bis-quaternary ammonium compounds (QACs).

Enhanced biosorption of cadmium ions on immobilized surface-engineered yeast using cadmium-binding peptides.

A new type of cadmium (Cd) ion cell surface adsorbent was developed by integrating bacteriophage display peptide library technology with cell surface display technology. Cd chelating resin served as the target molecule in screening experiments, leading to the identification of four Cd -binding peptides. These peptides were introduced into via the pYD1 plasmid using lithium acetate heat shock transformation.

The genome sequence of a non-capsular natural strain of A.Br.001/002 canSNP group isolated from permafrost in Yakutia, Russia.

We present the results of the whole-genome sequencing of a strain isolated from a permafrost sample collected in Yakutia, Russia. This strain was named YakM12. Phylogenetic analysis showed that YakM12 belongs to the canSNP group A.