Target-induced recycling and self-folding hairpin primer-mediated LAMP activation of CRISPR/Cas12a for highly sensitive aptamer-based therapeutic antibody assay.

Owing to their high affinity and specificity for antigen target molecules, therapeutic monoclonal antibodies (mAbs) have been increasingly used for the treatment of different diseases. The sensitive and accurate detection of mAbs is crucial for the evaluation of their efficacy and safety. With a new design of a thiophosphate-modified and self-folding hairpin primer, herein, we described the establishment of an aptamer-based, highly sensitive and simple fluorescent trastuzumab mAb assay method target-induced recycling and low-temperature LAMP activation of CRISPR/Cas12a signal amplifications.

The impact of digital technology use on EFL students' English academic performance: The mediating roles of emotional intelligence and learning engagement.

Background: For English as a Foreign Language (EFL) learners, English academic performance serves as a key determinant of their educational attainment and psychological well-being. While previous studies have pinpointed digital technology use, emotional intelligence, and learning engagement as key determinants of English academic performance, the specific mechanisms through which digital technology use impacts EFL students' English academic performance via emotional intelligence and learning engagement remain underexplored.

Methods: This study employed random sampling to investigate how digital technology use affects the academic performance of EFL students, with a particular focus on the mediating roles of emotional intelligence and learning engagement.

Exploring the link between BMI and physical fitness in Sichuan's ethnic minority primary school students: insights from quantile regression analysis.

Background: Although the nexus between Body Mass Index (BMI) and the physical fitness of the younger demographic has been well-documented, a comprehensive examination of this relationship among children from ethnic minority communities remains unexplored. This study aims to delineate the association between BMI and physical fitness among primary school students in Sichuan's ethnic minority regions, offering insights to inform health policy tailored to these communities.

Methods: Utilizing a randomized cluster sampling approach, the study was conducted across ethnic minority regions in Sichuan Province from September to October 2023, encompassing a cohort of 10,800 students from Grade 3 to Grade 6.

Assessment of resilience and key drivers of Tibetan villages in Western Sichuan.

This study employs an integrated analytical framework combining the Social-Ecological System (SES) and Driver-Pressure-State-Impact-Response (DPSIR) models, supplemented by quantitative methodologies including the Entropy Weight Method (EWM), Generalized Additive Model (GAM), Lasso regression, and shallow neural networks, to conduct a systematic resilience assessment of six representative Tibetan villages in western Sichuan based on longitudinal data from 2015 to 2022. The analysis reveals significant differences in resilience levels among the villages, primarily influenced by multiple factors. Disposable income and employment are key positive drivers that enhance adaptive capacity and resilience.

Physical activity levels and externalizing problem behaviors in Chinese adolescents using latent profile analysis.

The study aims to explore the relationship between physical activity (PA) levels and externalizing problem behaviors among Chinese adolescents, to inform personalized intervention strategies based on scientific evidence. This cross-sectional study was conducted from November 10 to December 15, 2024, among middle and high school students in Sichuan, Guangdong, Shandong, Henan, and Jiangxi provinces in China. A total of 1,718 valid questionnaires were collected (mean age = 14.

The relationship between job stress, resilience, emotional intelligence, and job satisfaction among college teachers.

College teachers face significant psychological challenges, such as high job stress and low emotional intelligence, which impact their job satisfaction and mental health. This study, guided by resilience theory and conservation of resources theory, explores how job stress and emotional intelligence affect job satisfaction, with resilience acting as a mediator and gender as a moderator. A cross-sectional study was conducted using an online questionnaire platform, with 1020 college teachers randomly selected to participate.

Genome-wide identification and characterization of the NF-Y proteins in .

Somatic embryogenesis from nucellar tissues is common in many plants. The nuclear factor Y (NF-Y) transcription factor is pivotal in this process. Despite its significance, the identification and functional analysis of the NF-Y transcription factor family in () remains unexplored.

Chrysosplenetin B suppresses the growth of human prostate cancer cells by inducing G1 cell cycle arrest.

Introduction: Prostate cancer (PCa) often progresses to castration-resistant prostate cancer (CRPC), which is linked to higher treatment resistance and recurrence rates. This highlights the urgent need for new therapeutic options. Natural products, especially flavonoids, have shown promise in reducing drug resistance and possess both antioxidant and anticancer effects.

Reinsurance-investment game between two α-maxmin mean-variance insurers.

This paper examines a non-zero-sum stochastic differential reinsurance-investment game between two competitive insurers under the [Formula: see text]-maximin mean-variance criterion. Both insurers can purchase proportional reinsurance and invest in a financial market consisting of one risk-free asset and one risky asset, and each insurer is concerned with its terminal surplus and relative performance compared to its competitor. The insurers aim to maximize the [Formula: see text]-maximin mean-variance utility, which allows them to exhibit different attitudes towards model ambiguity.

The Potential of MN-GPs (M = Mn, Fe, Co, Ni, Cu, Mo) as Adsorbents for the Efficient Separation of CH from CO and HS.

Carbon dioxide (CO) and hydrogen sulfide (HS) as harmful gases are always associated with methane (CH) in natural gas, biogas, and landfill gas. Given that chemisorption and physisorption are the key gas separation technologies in industry, selecting appropriate adsorbents is crucial to eliminate these harmful gases. The adsorption of CH, CO, and HS has been studied based on the density functional theory (DFT) in this work to evaluate the feasibility of transition metal (M = Mn, Fe, Co, Ni, Cu, Mo) porphyrin-like moieties embedded in graphene sheets (MN-GPs) as adsorbents.

Analysis of the response mechanism in lipid degradation of key gene LDH1 knockout strains of Saccharomyces cerevisiae under levulinic acid stress.

Levulinic acid (LA) is the main toxic by-product in the production of fuel ethanol, and its large-scale emission adversely affect the ecological environment. In order to effectively remove LA from the liquid waste, microbial degradation methods are adopted but the challenge is that microorganisms cannot fully tolerate LA in the waste. Therefore, it is particularly important to explore the tolerance mechanism of microorganisms to LA.

Combined effects of quinic acid and isochlorogenic acid B on LPS-induced inflammation and pyroptosis in MAC-T cells and mouse mammary glands.

Introduction: Quinic acid (QA) and isochlorogenic acid B (ICAB) have been demonstrated to possess antioxidant, anticancer, and anti-inflammatory properties. However, their combined efficacy in protection against mastitis requires further investigation. This study aims to examine the inhibitory effects and mechanisms of QA and ICAB combination on lipopolysaccharide (LPS)-induced inflammation and pyroptosis in bovine mammary epithelial cells (MAC-T) and mouse mammary tissue.

Associations Among Depressive and Anxious Symptoms, Fear of Missing Out, and Problematic Smartphone Use Severity Among Chinese Adolescents: A Three-Wave Random-Intercept Cross-Lagged Panel Analysis.

Purpose: Previous research has documented that problematic smartphone use (PSU) is associated with various psychological symptoms among adolescents, but temporal ordering and underlying mechanisms remain unclear.

Patients And Methods: This three-wave longitudinal study examined bidirectional relationships among depressive and anxious symptoms, fear of missing out (FOMO), and PSU in a sample of 402 Chinese adolescents (49.6% girls; = 12.

From quality of life to sleep quality in Chinese college students: stress and anxiety as sequential mediators with nonlinear effects via machine learning.

Object: This study examines how quality of life is associated with sleep quality among Chinese university students through sequential mediation by perceived stress and sleep anxiety, using machine learning to uncover nonlinear effects.

Methods: A cross-sectional sample of 7128 university students (aged 18-28) from Eastern, Central, and Western China was recruited via stratified random and snowball sampling for regional diversity. Ordinary least squares regression and bias-corrected bootstrapping (5000 resamples) assessed direct and sequential mediation effects of perceived stress and sleep anxiety, estimating indirect effects with 95 % confidence intervals.

Single-cell RNA sequencing uncovers cellular heterogeneity of granulosa cells and provides a signature for follicular development in chicken.

Functional differentiation of the granulosa cell (GC) layer plays an essential role in follicular development and oocyte maturation in birds. However, despite the importance of GCs, heterogeneity within the granulosa layer across poultry species remains poorly defined and functional transcriptomic signatures across distinct cellular subpopulations are lacking. In this study, single-cell RNA sequencing was used to identify GC types, uncover heterogeneity, and construct the developmental trajectories of chicken GCs at two developmental stages: the hierarchical follicle (HF)-GC and prehierarchical follicle (PHF)-GC stages.

Generate vector graphics of fine-grained pattern based on the Xception edge detection.

Harnessing the power of artificial intelligence(AI) approaches to innovatively generating the vector graphics of fine-grained patterns has become an important task in image edge extraction, particularly on the domain of intangible cultural heritage (ICH) images where they are typically fine-grained and having the complex edges. With higher autonomy, the machine learning algorithms are able to accurately extract the image information, understand and convey the concept contained in it. In this paper, we take Qiang embroidery patterns as an example due to containing fine-grained patterns, which is more suitable for the study of image processing and pattern recognition techniques.

C3-Formylation of Pyridines via Streptocyanine Intermediates.

Herein we develop a one-pot, multistep strategy for -selective C-H formylation of pyridines via streptocyanine intermediates. By combining aromatic nucleophilic addition, ring-opening, formylation, and ring-closing, nicotinaldehyde derivatives were efficiently synthesized. This method overcomes the limitations of traditional Vilsmeier-Haack reactions, offering a mild and versatile approach for functionalizing electron-deficient pyridines.

Identification of core microbial communities and their influence on flavor-oriented traditional fermented sour cucumbers.

Sour cucumber is a traditional fermented vegetable with global popularity, yet its fermentation process often leads to inconsistencies in quality and flavor due to the reliance on natural fermentation. This study identifies 12 core volatile organic compounds (VOCs) contributing to its unique flavor and investigates the key microbial species involved in the fermentation process. Using a synthetic microbial consortium constructed from core microbial species, we successfully replicated the flavor profile of naturally fermented sour cucumbers while enhancing safety by reducing nitrite levels.

Research on the capability, opportunity, and motivation of schools to conduct physical activities in China: a cross-group validation.

Background: Insufficient physical activity among adolescents is a major global public health concern, and schools are considered key venues for promoting physical activity. Teachers play a crucial role in the implementation of policies. This study adapted and validated the COM-PASS scale, based on the COM-B model, to assess its reliability and validity in the Chinese context.

Somatic symptom burden, PTSD, and dissociation: Cross-sectional findings from 995 international female mental health service users.

Objective: Somatic symptom burden is frequently linked to trauma-related psychopathology; Yet, the specific contributions of PTSD, disturbances in self-organization (DSO), and dissociation remain underexplored. Our study examined the prevalence of somatic symptom burden among female mental health service users and evaluated the associations between trauma-related symptoms and somatic symptom burden.

Methods: Female participants (N = 995) from international clinical settings completed validated self-report measures assessing somatic symptoms (SSS-8), childhood trauma (BBTS), PTSD and DSO symptoms (ITQ), and dissociation (MDI).

Isolation of highly efficient potassium solubilizing bacteria and their effects on nutrient acquisition and growth promotion in tobacco seedlings.

Background: Tobacco plants are typically high potassium (K)-demanding during growth and development, but the bioavailability of the nutrient in tobacco-growing soils is often limited.

Aim And Methodology: The present study aimed at screening highly efficient potassium-solubilizing bacteria (KSB) through in vitro experiments, and to grasp their potential role in mineral dissolution for nutrients release. The effects of these bacterial inoculants on soil nutrient bioavailability, macronutrient acquisition, soil bacterial community characteristics, and tobacco seedling biomass were investigated through a greenhouse pot experiment.

An alkali-halophilic laccase facilitates highly efficient conversion of lignin into polyhydroxybutyrate.

Lignin valorization is crucial for sustainable biorefineries, yet its complex structure challenges efficient conversion. Here, we report an alkali-halophilic laccase (Lacc) from Halomonas sp. Y3 that enables highly efficient lignin-to-polyhydroxybutyrate (PHB) conversion.

Research on equipment fault diagnosis model based on gan and inverse PINN: Solutions for data imbalance and rare faults.

In the field of medical imaging equipment, fault diagnosis plays a vital role in guaranteeing stable operation and prolonging service life. Traditional diagnostic approaches, though, are confronted with issues like intricate fault modes, as well as scarce and imbalanced data. This paper puts forward a fault diagnosis model integrating digital twin technology and Inverse Physics - Informed Neural Networks (Inverse PINN).

3D visualization design of digital intelligent landscape environment based on wireless network security.

The digital revolution is sweeping all areas of human life at an unprecedented speed. Landscape design, as an important discipline that affects people's quality of life, is also undergoing a digital transformation. This transformation has brought about different modes of thinking and expressions.