GreenCells: A comprehensive resource for single-cell analysis of plant lncRNAs.

Long non-coding RNAs (lncRNAs) play crucial roles in plant growth, development, and stress responses. With the advancement of single-cell RNA sequencing (scRNA-seq) technology, it is now possible to investigate lncRNA expression and function at single-cell resolution. Although several plant single-cell transcriptome databases have been established, they predominantly focus on protein-coding genes while largely overlooking lncRNAs.

Manganese accumulation characteristics and rhizosphere decontamination mechanisms of Phytolacca icosandra L.

The role of microbial metagenomics in understanding ecological changes associated with rhizosphere heavy metal decontamination by plants has often been overlooked. The aim of this study was to scrutinize the structural, enzymological and metagenomic mechanisms leading to manganese (Mn) decontamination in the rhizosphere by Phytolacca icosandra. Seedlings of P.

The polyphasic approach reveals two new species and two new records of (Apiosporaceae, Amphisphaeriales) associated with from China.

This study isolated endophytic fungi of the genus from (Chinese agarwood) in Guangxi, China. Through rigorous morphological comparisons and molecular phylogenetic analyses, we have identified two new species ( and ) and two new host-recorded species ( and ). The morphological analysis revealed that the new species align with the genus-level definition of in colony morphology, conidiophores, and conidial characteristics.

A global meta-analysis of floral longevity reveals widespread plasticity and ecological correlates.

Plasticity of floral longevity (FL) in response to pollination enables plants to maximize opportunities for pollen receipt and export under unfavorable pollination conditions, while minimizing costs under favorable ones, playing a crucial role in plant pollination. However, the prevalence of floral longevity plasticity (FLP) across the angiosperms remains unclear. Using data for FL consisting of 397 comparisons from 188 species, we provide the first global, cross-species quantification of FLP and assess its association with a range of biotic and abiotic factors.

Chromosome-level genome assembly of Hippophae salicifolia.

Hippophae salicifolia, a dioecious small tree species endemic to the Himalayan region, holds great potential in both ecological conservation and industrial applications. In this study, we employed PacBio HiFi long reads, Illumina short reads, and Hi-C technology to construct a high-quality, chromosome-level reference genome. The assembled genome is approximately 1.

High-resolution imaging of plant delayed luminescence.

Delayed luminescence (DL) is a quantized signal that is characteristic of photoexcited molecules entering a relaxed state. Studying DL provides critical insight into photophysical mechanisms through the analysis of specific spatiotemporal dynamics. In this study, we developed a high-sensitivity DL imaging system using a quantitative scientific complementary metal-oxide-semiconductor (qCMOS) camera and a single-photon counting resolution.

New Insights into the Structural Profiling of Polyphenol Aggregates from Pu-erh Tea and Their Anti-Inflammatory Activity the TLR4/MyD88/NF-κB Signaling Pathway and Gut Microbiota.

Pu-erh tea polyphenol aggregates (PTPAs), macromolecular complexes analogous to theabrownin formed during microbial fermentation, are key bioactive components in this tea. Although their bioactivities are documented, critical gaps persist regarding both precise structural features and molecular mechanisms underlying the anti-inflammatory efficacy across biological models. To address these limitations, we systematically analyzed PTPAs using integrated methods, including alkaline hydrolysis, acid hydrolysis, and benzyl mercaptan-mediated degradation.

Structurally diverse diterpenoids from cones of Taxodium ascendens inhibit colorectal cancer proliferation via regulating mitophagy.

Taxascendins A - D (1-4), four unprecedented hetero-oligomeric terpenoids, and taxascendins E - K (5-11), seven new diterpenoids with five distinct and highly modified abietane-types frameworks, along with sixteen known analogues (10-27) were obtained from Taxodium ascendens. Their structures were determined by extensive spectroscopy, single-crystal X-ray diffraction, and quantum chemical calculations. Bioactivity screening indicated that the isolated compounds exhibited inhibitory activity in colorectal cancer cells.

Molecular and Morphological Evidence Reveals Four New Species from Dragon Trees in Yunnan Province, China.

() is a globally distributed fungal genus, traditionally recognized as a group of plant pathogens, with most members known to cause severe plant diseases. However, recent studies have demonstrated that many of these fungi can also colonize plants endophytically, with certain strains capable of promoting plant growth and stimulating the production of secondary metabolites. In this study, 13 strains of were isolated from the stems and leaves of and in Yunnan Province, China.

Omics Integration Reveals Hormonal Adjustments in Irrigated and Nonirrigated Rice Varieties Upon Mythimna separata Feeding.

Freshwater consumption is the biggest challenge in irrigated rice cultivation (Oryza sativa L.). Nonirrigated farmland rice cultivation is a strategy to mitigate the water resource shortage associated with traditional irrigated rice farming.

Deinococcus lichenicola sp. nov., A Bacterium Isolated from a Lichen Sample.

A Gram-stain-positive bacterium, designated as YIM 134068, was isolated from a lichen. Strain YIM 134068 was non-motile, non-spore forming, coccus shaped, oxidase, and catalase positive. Cells could grow at 10-40 °C (optimum 28 °C) and pH 7.

Ericoid mycorrhizal fungal metacommunity facilitates closely related Rhododendron species coexistence.

The structural and ecological significance of ericoid mycorrhizal (ErM) fungi is well recognized. Yet, it remains unknown whether the coexistence of ericaceous plants is facilitated by their symbioses with ErM fungi. Here, we use Rhododendron (Ericaceae) as a model to investigate how phylogenetic and functional limiting similarity, or facilitation through ErM fungal metacommunity, influences their co-occurrence in mountain ecosystems.

ZmPP2C45 and ZmBELL4 suppress maize biochemical defense against insect herbivores.

Benzoxazinoids (BZX) are the most abundant defensive metabolites of maize (Zea mays). Genetic fine-tuning of BZX metabolism holds the potential to enhance maize resistance against insect herbivory. Natural variation in BZX abundance has been associated with genetic polymorphism in ZmPP2C45.

The ethnomedicinal, pharmacological, and phytochemical potential of Ferula sinkiangensis K.M.Shen: An updated and comprehensive review.

Ethnopharmacological Relevance: Ferula sinkiangensis, commonly known as "Chinese Awei" or "Chinese Ferula", has attracted considerable attention due to its diverse ethnobotanical uses and phytochemical complexity. However, lacking an updated and comprehensive review addressing ethnobotany, phytochemistry, pharmacology, and threats facing this critically endangered medicinal plant of Xinjiang, China.

Aim: This comprehensive review aims to provide an updated, meticulous study of F.

HEP14-activated PKC-ERK1/2 pathway boosts HEP14-empowered hADSCs for ovarian regeneration and functional restoration.

Premature ovarian insufficiency (POI) and age-related natural-aging ovarian insufficiency (ARNA-OI) pose pressing global health challenges, necessitating effective therapeutic strategies and a deep understanding of their underlying mechanisms. This study investigates how HEP14, a PKC pathway activator, boosts the regenerative potential of human adipose-derived stem cells (hADSCs) for ovarian regeneration. Transcriptome analysis reveals that HEP14 modulates gene expression profile in hADSCs, enhancing their regenerative capacity.

Noncanonical terpene cyclases for the biosynthesis of diterpenoids regulating chlamydospore formation in plant-associated Trichoderma.

Terpene cyclases catalyze exquisite and complicated cyclization reactions to generate diverse terpenoid skeletons. Trichoderma fungi are important biocontrol agents, characteristic of producing complex bioactive tetracyclic diterpenoids named harzianes and trichodermanins, but their biosynthesis and biological functions have long been enigmatic. Here we identify TriDTCs, an unprecedented family of terpene cyclases in Trichoderma, responsible for cyclizing geranylgeranyl diphosphate (GGPP) into major diterpenes harzianol I and wickerol A, via heterologous expressions, gene deletion, and in vitro assays.

Irregular Terpenoids From the Medicinal Herb Gentianella pygmaea.

Three new irregular terpenoids, gentianellins A-C (1-3), along with two known analogues (4 and 5), were isolated from the whole plants of the medicinal herb Gentianella pygmaea. Their structures were elucidated by extensive spectroscopic analysis (1D and 2D NMR and HRMS) and quantum chemical calculations. Moderate anti-inflammatory activity of the new isolates was observed via suppressing the secretion of cytokines TNF-α and IL-6 in LPS-induced macrophages RAW264.

Elucidation of the biodegradation pathways of polyurethane and polyethylene terephthalate by a Fusarium strain enriched from soil-plant systems.

Fungi are emerging as a promising solution for bioremediation. Several fungi have been identified to degrade polyester, but the specific molecular mechanisms involved remain poorly understood. In this study, we screened for polyester-degrading fungus, characterised its ability to depolymerise both polyurethane (PU) and polyethylene terephthalate (PET), and employed a multi-omics approach to identify key proteins involved in PU and PET hydrolysis.

Immunosuppressive spirocyclic labdane diterpenoids from Holocheila longipedunculata.

Phytochemical study on Holocheila longipedunculata has revealed twenty previously undescribed spirocyclic labdane diterpenoids, named hololabdanes (1-20). Their chemical structures were elucidated through comprehensive spectroscopic analysis, and the absolute configuration of hololabdane A (1) was determined via single-crystal X-ray diffraction. Notably, hololabdane I (17) and 16-epi-hololabdane I (18) feature a rare pyrrolo [2,1-b] oxazolidinone moiety, while hololabdane J (19) and 2'-epi-hololabdane J (20) possess a distinctive peroxide bridge.

Characterization of the complete plastid genome sequence of Á.J. Pérez & E. Rea (Magnoliaceae), a newly described species endemic to the Ecuadorian Andes.

Á.J.Pérez & E.

A new revision of the lichen genus (Acarosporales, Acarosporaceae) in China reveals two new species.

Based on a combination of morphological, chemical, and phylogenetic analyses, we present a new revision of the lichen genus in China. and are proposed as new species to science, both included in a distinctive clade (clade 1) together with . These three species are characterized by the absence of fatty acids, acaranoic and acarenoic acids, and share similar ecological traits.

Discovery of lignans from leaves of Phyllanthus emblica L. and their antioxidant-antiinflammatory activity.

Seventeen lignans (1-17), including five new ones (1-5), were identified from the ethanolic extract of Phyllanthus emblica leaves, ten of which were isolated for the first time from the titled plant. Their structures were meticulously elucidated through comprehensive analysis utilizing UV, IR, NMR (1D and 2D), HRESIMS, ECD calculations, and comparison with previous literature data. The lignans 5, 8, 10, and 15-17 exhibited DPPH radical scavenging activity with IC values of 10.

DNA methylation valley as a distinguishing feature occurs in root-specific expressed nicotine-related genes in .

Introduction: Nicotine, the main defense alkaloid of species, is synthesized exclusively in the roots. Several studies have shown that changes in DNA methylation patterns are associated with altered expression of genes involved in the biosynthesis of some secondary metabolites. It remains unknown whether DNA methylation pattern of nicotine-related genes differs in root and leaf tissues.

 (Polypodiales, Athyriaceae), a new fern species from southern Yunnan, China.

A new species in Athyriaceae, (section Anisogonium) is described and illustrated from southern Yunnan, China. exhibits the greatest morphological similarity to , particularly in lamina division, but can be reliably differentiated, based on lamina width and lobe shape. Molecular phylogenetic analysis demonstrates that the new species is closely related to , despite low morphological concordance.