Advancing Enzyme-Based Detoxification Prediction with ToxZyme: An Ensemble Machine Learning Approach.

The aaccurate prediction of enzymes with environment detoxification functions is crucial, not only to achieve a better understanding of bioremediation strategies, but also to alleviate environmental pollution. In the present study, a novel machine learning model was introduced which classifies enzymes by their toxin degradation ability. In this model, two different sets of data were used which include enzymes that can catalyze the toxin degradation as a positive dataset and non-toxin-degrading enzymes as a negative dataset.

Investigation of standardized ileal amino acid digestibility of rice protein meal in sexed broilers.

Rice protein meal (RPM), a byproduct of the rice industry, has emerged as a potential alternative for poultry feed, especially in rice-producing regions in the South Asia region. The aim of this study was to evaluate the standardized ileal amino acid (AA) digestibility of RPM produced from two distinct rice varieties in sexed broiler chickens, with or without the inclusion of a commercial protease enzyme. Treatments were structured in a 2 × 2 × 2 factorial arrangement, including two sources of RPM (Basmati and IRI), sex (male and female), and protease levels (0 and 500 units per ton of feed).

Extraction and Analysis of Natural Color From Beetroot ( L.) Using Different Techniques, and Its Utilization in Ice Cream Manufacturing.

A rich source of natural color, beetroot (Beta vulgaris L. subsp. vulgaris) extracts and colorants offer deep red color to the dishes and boost their nutritional and antioxidant value.

Bi-allelic pathogenic variants in TRMT1 disrupt tRNA modification and induce a neurodevelopmental disorder.

The post-transcriptional modification of tRNAs plays a crucial role in tRNA structure and function. Pathogenic variants in tRNA-modification enzymes have been implicated in a wide range of human neurodevelopmental and neurological disorders. However, the molecular basis for many of these disorders remains unknown.

Optimization of a multiplex PCR assay for simultaneous detection of key bovine respiratory infections.

The control of respiratory infections is integral to a healthy livestock farm. The current study was conducted to optimize a multiplex PCR assay for simultaneous detection of Pasteurella multocida (P. multocida), Staphylococcus aureus (S.

Thymoquinone loaded zinc oxide Nanoformulations synthesis, characterization and evaluation of their efficacy against carbon tetrachloride induced Hepatorenal toxicity in rats.

Thymoquinone (THQ), a strong antioxidant and anti-inflammatory bioactive compound has been reported in numerous studies to prevent the hepatorenal toxicity caused by various xenobiotics. Similarly, the zinc oxide nanoparticles (ZnONPs) have been used to protect against the hepatorenal damages caused by oxidative stress due to their potent antioxidant properties. The aim of this study was to synthesize and investigate the possible protective effects of THQ, ZnONPs and THQ-loaded ZnONPs against CCl induced hepatorenal toxicity in albino rats.

A new level of RNA-based plant protection: dsRNAs designed from functionally characterized siRNAs highly effective against Cucumber mosaic virus.

RNA-mediated crop protection increasingly becomes a viable alternative to agrochemicals that threaten biodiversity and human health. Pathogen-derived double-stranded RNAs (dsRNAs) are processed into small interfering RNAs (siRNAs), which can then induce silencing of target RNAs, e.g.

Molecular epidemiology and antibiotic resistance profiling of Staphylococcus aureus isolates from camel mastitis.

Mastitis is considered one of milk-producing animals' most widespread infectious diseases. The present study evaluated the prevalence of antibiotic-resistant isolates of Staphylococcus aureus (S. aureus) including methicillin-resistant S.

Effect of Hepatitis C Infection on Semen Parameters of Men From Rawalpindi and Islamabad, Punjab, Pakistan.

Hepatitis C virus (HCV) poses a global health challenge, especially due to its genetic diversity and capacity to infect 71 million people worldwide. This infection is prevalent in Pakistan, with 15% of the population affected, with varying rates across provinces. HCV primarily targets liver cells but can also impact other bodily systems, including the reproductive system.

Immunomodulatory and growth-promoting effects of root powder in broiler chicks challenged with Gallinarum.

Background: Amid growing concern about antimicrobial resistance due to the irrational use of antibiotics in treating common poultry diseases, particularly which is a foodborne pathogen in humans. This study investigates the effects of ethnoveterinary supplementation of (L. Benth.

-Carrageenan Manganese Oxide Bionanocomposites as a Promising Solution to Agricultural Challenges.

Agriculture faces numerous challenges: infectious diseases through phytopathogens and soil nutrient deficiencies hinder plant growth, reducing crop yields. Biopolymer nanocomposites offer promising solutions to these challenges. In this work, we synthesize and characterize novel bionanocomposites (-CG-Mn) of manganese (hydr)oxide nanoparticles (approx.

Unravelling anti-cancer properties of solanaceous extracts using GC-MS and HPLC.

Cancer is a complex health issue that today's medical science is dealing with, which has a mortality rate that is almost identical to that of cardiac disorders. Because of the adverse effects of the majority of the medications used in its therapy, managing it remains a major concern despite the availability of numerous remedies. This article attempts to contribute to the scientific developments in the Solanaceae family's anti-cancer field.

Fragment optimized chalcone derivatives targeting OmpA protein as a therapeutic approach against multidrug resistant Acinetobacter baumannii.

Acinetobacter baumannii is a notorious pathogen associated with life-threatening infections, with its outer membrane protein A (OmpA) being a key contributor to its pathogenicity by targeting epithelial cell apoptosis. The study presents an in silico analysis of chalcone derivatives as potential therapeutic agents against the outer membrane protein A (OmpA) of Acinetobacter baumannii. We performed molecular docking to evaluate the binding interactions, revealing that isobavachalcone exhibited the highest binding affinity.

The measurement of microplastics in surface water and their impact on histopathological structures in wading birds of district Lahore.

Plastics are globally considered a significant threat, particularly to metropolitan areas, due to the extensive use of plastic products. This research is the first of its kind to document microplastics contamination and its effects on Red wettled lapwing (Vanellus indicus). The concentration of microplastics (MPs) was measured from surface water at different locations including canals and drains, which are the primary sources of MPs pollution in the metropolitan city Lahore, Pakistan.

The Impact of Chemotherapy on the Nutritional Status of Breast Cancer Patients.

Background: Breast cancer is one of the most common cancers among Pakistani women. It is mostly diagnosed at stage 2, requiring chemotherapy in certain cases. Chemotherapy is of two types: adjuvant and neoadjuvant.

Effects of dietary garlic () and papaya () leaf powder on production performance, ruminal methanogen levels, gut parameters, and meat quality in goats.

Background And Aim: Several approaches have been employed to mitigate methane emissions from livestock, with varied results. This study evaluated the effects of shade-dried ground garlic leaf (GL) powder and papaya leaf (PL) powder as crop waste on feed intake, growth performance, ruminal microbial counts, gut epithelial barrier functions, and meat quality in goats.

Materials And Methods: Forty male adult Beetal goats were randomly divided into five treatment groups: (1) Control (basal diet only); (2) basal diet supplemented with 6% bromodichloromethane (BCM); (3) basal diet supplemented with 30% GL powder; (4) basal diet supplemented with 26% PL powder; and (5) basal diet supplemented with 30% GL powder and 26% PL powder (GP).

Cyto-Genotoxic Assessment of Sulfoxaflor in Allium cepa Root Cells and DNA Docking Studies.

Sulfoxaflor (SFX) is an insecticide that is commonly used for the control of sap-feeding insects. Since SFX is extensively applied globally, it has been implicated in the substantial induction of environmental toxicity. Therefore, in this study, Allium cepa roots have been employed to elucidate the potential cytogenotoxic effects of SFX in non-target cells by examination of mitotic index (MI), chromosomal aberrations (CAs), and DNA damage.

Effect of human chorionic gonadotropin on oocyte maturation and developmental competence in buffalo.

We hypothesized that human chorionic gonadotropic (hCG) could replace LH in the maturation media for buffalo oocytes, and hCG administration before ovum pick-up (OPU) enhances in-vitro development of buffalo oocytes. Objectives were 1) to investigate the effect of hCG supplementation on nuclear maturation, oocyte development, and granulosa cell mRNA abundance of genes related to growth and antioxidant pathways and 2) to determine the effect of hCG administration before OPU on in-vitro oocyte development. In Experiment 1, buffalo oocytes retrieved from slaughterhouse ovaries were maturated in the media supplemented with 0.

A Novel Homozygous Loss-of-Function Variant in GPR156 Delineates Non-syndromic Hearing Loss.

Non-syndromic hearing loss (NSHL) is a genetically heterogeneous disorder accounting for almost 70% of the total congenital hearing loss. The implementation of rapid advanced sequencing methods has significantly contributed to the correct molecular diagnosis for several rare genetic disorders, including NHSL. Features of two probands with NHSL were clinically and genetically evaluated.

Antimicrobial resistance, virulence profiling, and drug repurposing analysis of Staphylococcus aureus from camel mastitis.

Camel mastitis especially caused by Staphylococcus aureus (S. aureus), is a major risk to animal health and milk production. The current investigation evaluated the antibiotic susceptibility and virulence factors of S.

Clinical and genetic characterization of a progressive RBL2-associated neurodevelopmental disorder.

Retinoblastoma (RB) proteins are highly conserved transcriptional regulators that play important roles during development by regulating cell-cycle gene expression. RBL2 dysfunction has been linked to a severe neurodevelopmental disorder. However, to date, clinical features have been described in only six individuals carrying five biallelic predicted loss-of-function (pLOF) variants.

MS -Pushing the Limits for Biomolecular Mass Spectrometry.

Electrospray mass spectrometry has become indispensable in many disciplines including the classic "omics" techniques such as proteomics or lipidomics, as well as other life science applications in molecular, cellular, and structural biology. However, a limiting factor that often arises for the detection of biomolecular analytes is their poor ionization efficiency in the ion source. Here, we present an add-on device for the electrospray source, termed MS (MS Spectral Impurity Eliminator & Value Enhancer), which is placed between the electrospray needle and the cone of the mass spectrometer.

Designing a multi-epitope construct using immuno-informatic tools to prepare a messenger RNA vaccine against ticks.

Background And Aims: Ticks are blood-feeding ectoparasites that transmit pathogens to animals and humans. One of the most important hard ticks in animals is , which transmits and spp. Although many potential tick vaccine candidates have been identified, no effective vaccine that can provide sterile immunity against tick infestations has been developed.

A comparative study of the microbiomes of the ticks Rhipicephalus microplus and Hyalomma anatolicum.

Hyalomma anatolicum and Rhipicephalus microplus are tick species that are important vectors of numerous pathogens affecting both humans and livestock. Endosymbionts, such as Coxiella-like endosymbionts (CLE), Francisella-like endosymbionts (FLE), and Candidatus Midichloria, play a crucial role in the physiology and vector competence of these ticks. In this study, we investigated the microbial composition of H.

Extracellular lipase production from Bacillus cereus by using agro-industrial waste.

Lipases are crucial biocatalysts in various industrial applications, and there is considerable interest in developing sustainable methods for their synthesis. This study focuses on the isolation, screening, and comparison of Bacillus cereus strains to produce extracellular lipases utilizing agro-industrial waste through solid-state fermentation. The results indicate that B.