Molecular characterization and green synthesis of iron oxide nanoparticles from Ureibacillus chungkukjangi for methylene blue degradation.

Synthetic dyes, such as methylene blue (MB), are increasingly becoming sources of water pollution and require better treatment strategies. This study describes an eco-friendly method for methylene blue degradation using green synthesized iron oxide nanoparticles form Ureibacillus chungkukjangi. This bacterium was isolated from clinical samples and identified using 16S rRNA gene amplification and sequenced using Sanger sequencing technology.

Mechanisms of the Ershiwuwei Guijiu Pill in Treating Postmenopausal Osteoporosis Based on Network Analysis and Experimental Validation.

BackgroundThe Tibetan medicine Ershiwuwei Guijiu Pill (EWGP), a classic Tibetan medicine prescription for the treatment of postmenopausal osteoporosis (PMOP) in the Qinghai-Tibet region, has attracted extensive attention due to its curative effects on gynecological diseases. However, its chemical ingredients and molecular mechanism are still unclear.Aim of the studyTo analyze the chemical constituents and effective serum chemical metabolites of EWGP and to explore the molecular mechanism of EWGP in treating PMOP through network analysis and experimental validation.

Trends and disparities in heart failure and heart failure with obesity mortality among U.S. adults: A 1999-2020 analysis of geographic, gender, and racial variations using CDC WONDER data.

Background: Heart failure (HF) is a leading cause of hospitalization and mortality, and it poses a significant burden on healthcare systems globally. Obesity is a considerable risk factor for HF and contributes to increased cardiac stress and metabolic demands.

Methods: Data from the CDC WONDER database were examined from 1999-2020 for HF and HF with obesity-related mortality in adults aged 25 years and older at the time of death, using ICD-10 codes.

Wealth and regional disparities in child undernutrition: insights from national demographic and health survey.

Introduction: In developing countries like Pakistan, the prevalence of malnutrition embodies a multifaceted development challenge, intricately linked to structural inequalities, with disproportionate burdens among socioeconomically and geographically disadvantaged populations.

Methods: Drawing on the most recent Pakistan Demographic and Health Survey (2017-18), this study examines the magnitude of child undernutrition disparities across wealth quintiles and geographic regions. This study employs the Concentration Index (CI) with decomposition analysis, alongside Oaxaca-Blinder decomposition as robust.

Biochar-microbe synergy enhances auxin-mediated soil-plant interactions for canola productivity in alkaline calcareous soil.

Biochar and microbial bio-fertilizers, such as endophytic fungi and plant growth-promoting endophytes (PGPEs), offer sustainable alternatives to chemical fertilizers by enhancing soil fertility and plant performance. However, their synergistic effects particularly those involving auxin (IAA) biosynthesis and nutrient uptake, remain underexplored in calcareous soils. This study investigates how the integration of biochar with auxin-producing microbial inoculants influences soil-plant interactions and canola productivity.

Computational exploration of natural inhibitors against toxin-associated proteins in Naegleria fowleri Karachi strain.

Naegleria fowleri, a thermophilic, free-living amoeba, is the causative agent of Primary Amoebic Meningoencephalitis (PAM), a rare but nearly always fatal brain infection. The rising number of PAM cases in Karachi, Pakistan, particularly linked to a unique local strain, underscores the urgent need for effective therapeutic interventions. In this study, a computational approach was employed to identify potential natural inhibitors targeting toxin-producing proteins from the N.

Interplay between the morphology, crystal facets and silver modifications of titanium dioxide nanomaterials in the context of photocatalysis.

Titanium dioxide (TiO) having the morphology of nanofibers (TNFs), nanorods (TNRs), and nanograss (TNGs) nanostructures were prepared. Synthetic procedures had a great impact on the obtained morphology and phase. Nanomaterials were prepared under neutral conditions in the presence of ethylene glycol for TNFs, while those obtained under strongly basic conditions during the recrystallization process of the TiO powder exhibited the morphology of TNRs.

Colchicine as an Adjunct Therapy Postablation for Atrial Fibrillation: A Systematic Review and Meta-analysis.

Atrial fibrillation (AF) recurrence following catheter ablation remains a significant clinical challenge, often attributed to postprocedural inflammation. Colchicine, an anti-inflammatory agent, has emerged as a potential therapeutic strategy to reduce recurrence. This meta-analysis evaluates the efficacy and safety of colchicine in preventing AF recurrence after ablation.

Chronic Kidney Disease and Ischemic Heart Disease Mortality in the United States: A 25-Year Review of Racial and Geographic Disparities.

Chronic kidney disease (CKD) and ischemic heart disease (IHD) are leading causes of death in the United States, with a bidirectional relationship exacerbating morbidity and mortality. Despite advancements in treatment, disparities persist across demographic and geographic groups. This study examines trends in CKD- and IHD-related mortality from 1999 to 2023, assessing variations by age, sex, race/ethnicity, and geographic location.

Whole-Genome analysis of Bacillus subtilis MBBL2 genomic characterization and comparative genomics.

This study presents the whole-genome sequencing and comprehensive analysis of Bacillus subtilis MBBL2, a strain isolated from soil samples in Lahore, Pakistan. The genome consists of 4,574,405 base pairs, with a GC content of 42.99%, and is assembled into 908 contigs.

Role of metabolomic changes in diagnosis and assessment of treatment response in acute myeloid leukemia.

Acute Myeloid Leukemia (AML) is an aggressive cancer characterized by the rapid proliferation of abnormal myeloid cells. Despite advancements in treatment, patient outcomes remain variable, underscoring the need for more accurate diagnostic and prognostic tools. Metabolomics has gained attention for its potential to offer new insights into disease mechanisms and progression.

Stalled progress and emerging disparities in acute myocardial infarction mortality among U.S. older adults (> 65 Years) with renal failure, 1999-2023.

Background: Older adults with chronic kidney disease (CKD) experience disproportionately high mortality after acute myocardial infarction (AMI). Contemporary national trends-and their variation across demographic and geographic strata remain poorly defined.

Methods: Using the CDC WONDER Multiple Cause-of-Death files, we identified U.

Marine Drug Design for Epitheliocytis Rendering AI-Assisted Bioisosteric Replacement of Salicin.

Epitheliocystis is a distinctive and relatively understudied bacterial infection of finfish skin and gill epithelia that occurs intracellularly and results in the enlargement of the host cells. The Candidatus Syngnamydia salmonis is a bacterial pathogen that affects fish, especially salmon species like trout and salmon. The production of Epitheliocystis by Candidatus Syngnamydia salmonis poses several challenges to the aquaculture industry.

Polysaccharides Ameliorate DSS-Induced Colitis by Restoring Intestinal Barrier Function and Reprogramming Immune Homeostasis via the Gut-Liver Axis.

Ulcerative colitis (UC) is a chronic inflammatory bowel disease driven by immune dysregulation, microbiota imbalance, and intestinal barrier dysfunction. Despite its global burden, effective therapies remain limited. This study explores the therapeutic potential of polysaccharides (ACP) in a dextran sulfate sodium (DSS)-induced murine colitis model.

Thyroid Hormone Therapy for Potential Heart Donors: A Comprehensive Review of Clinical Trials.

Due to neurohormonal disturbances that occur following brain death, thyroid hormone therapy has been proposed as a means to enhance cardiac function in brain-dead organ donors. However, it remains unclear whether thyroid hormone administration improves clinical outcomes in potential heart donors. A comprehensive review of clinical trials was conducted to evaluate the impact of thyroid hormone therapy on heart viability and transplantation outcomes.

Advanced satellite-based remote sensing and data analytics for precision water resource management and agricultural optimization.

This study presents a novel integration of the Water Ratio Index (WRI), Normalized Difference Chlorophyll Index (NDCI), Land Use/Land Cover (LULC) mapping, and Cellular Automata-Markov (CA-Markov) modeling with temperature fluctuations to monitor irrigated land dynamics using high-resolution (30m) satellite imagery in South Africa's North West Province between 2016 to 2023, revealing critical challenges to agricultural sustainability and water resource management. Satellite imagery and geospatial analysis show irrigated lands concentrated in the region, which declined from 25,732 km to 24,322 km, while urbanization expanded built-up areas from 4,146 to 6,581 km, competing for arable land. The CA-Markov model predicts further agricultural loss by the year 2033, with barren land dominating (62.

Combination of Withania coagulans and Fagonia cretica ameliorates hyperuricemia by re-modulating gut microbiota-derived spermidine and traumatic acid.

Background: Hyperuricemia (HU), characterized by elevated serum uric acid (UA) levels, is associated with metabolic disorders, renal diseases, gout and gut dysbiosis. Withania coagulans (WC) and Fagonia cretica (FC) are medicinal plants traditionally used in Pakistan for their antioxidant and anti-inflammatory properties; however, their potential in HU management remains unexplored.

Purpose: This study investigates the efficacy of WC and FC crude extracts in ameliorating HU and explores the underlying mechanisms in mice models with HU.

Impact of diabetes mellitus type-2 on the outcomes following mitral transcatheter edge-to-edge repair (TEER): A meta-analysis.

Background: Diabetes mellitus (DM) has been linked to unfavorable outcomes in patients undergoing Mitral Transcatheter Edge-to-Edge Repair (TEER). Nevertheless, the literature contains conflicting data. This meta-analysis aimed to assess the impact of DM on outcomes following Mitral TEER.

In vitro and in silico study and pharmacokinetic analysis of the acaricidal effectiveness of Cersium arvense extract against Rhipicephalus microplus.

Rhipicephalus microplus, a tick species, causes significant economic losses in livestock and presents control challenges due to the emergence of resistance to conventional acaricides. This underscores the urgent need for effective and eco-friendly alternatives. This study evaluates the acaricidal potential of Cirsium arvense using adult immersion and larval packet tests.

Trends and disparities in ischemic heart disease mortality in the United States: an analysis of CDC WONDER database, 1999-2020.

Background: Ischemic heart disease (IHD) remains a leading cause of mortality globally and has a high prevalence in the USA, necessitating an understanding of long-term trends to inform interventions. Considering demographic and geographic disparities, this study examines IHD-related mortality trends among US adults from 1999 to 2020.

Aim: This study aimed to evaluate patterns and geographical variations in mortality associated with IHD among adults in the USA.

Localized brain stimulation with mild magnetic hyperthermia promotes microglia activity towards reactive and autophagic phenotypes in vivo.

The principle of magnetic hyperthermia is to generate localized heating on target proteins, cells and tissue that are targeted by magnetic nanoparticles (MNPs) upon stimulation by remotely applied high frequency alternating magnetic field (AMF). Beyond its traditional applications in hyperthermia therapy, recent studies demonstrated the feasibility of magnetic hyperthermia as a new strategy for neural stimulation. The objective of this study is to examine the feasibility of localized magnetic hyperthermia (i.

In silico investigation of ketamine and methylphenidate drug-drug conjugate for MDD and ADHD treatment using MD simulations and MMGBSA.

Major depressive disorder (MDD) and Attention-deficit hyperactivity disorder (ADHD) are pervasive psychiatric disorders characterized by impaired memory, sustained attention/motivation, and depression. FDA-approved Ketamine is an antidepressant drug but it affects cognitive abilities. Methylphenidate is the drug used for the treatment of ADHD.

Computational Design of a Multi Epitope Vaccine Against Staphylococcus warneri for Combatting Recurrent UTIs and Skin Infections.

Staphylococcus warneri is a pathogenic bacterium that causes multiple life-threatening diseases, such as urinary tract and skin infections globally, with approximately one million new cases reported each day. The World Health Organization (WHO) has classified it as a "superbug," and no licensed vaccine is currently assessable. In this study, we designed a vaccine employing B and T-cell epitopes from two highly antigenic membrane proteins of S.