Identification of as a potential source of antimycobacterial components.

is a medicinal plant from the Solanaceae family, having medicinal properties and some toxic effects. It is widely distributed across Asia, Africa, Europe, the Americas, and other tropical and subtropical regions, where it is utilized by local pharmaceutical industries. In this study, bioassay-guided fractionation and LC-MS/MS analysis were used for the identification of secondary metabolites with anti-tuberculosis activity in methanolic leaf extracts of .

Nutritional evaluation of extra virgin olive oil extracted from olive cultivars grown in Pothwar region of Pakistan.

There is negligible data available regarding nutritional evaluation of extra virgin olive oil (EVOO) obtained from olive cultivars from Pothwar region of Pakistan. The aim of this study was to profile nutritional parameters of EVOOs extracted from 10 olive cultivars of Pothwar. This was an experimental quantitative research design.

Urea adulteration alters raw milk composition and induces renal tissue damage: a molecular and histopathological study.

This study investigates the impact of urea adulteration in raw milk on milk quality, safety, and renal tissue integrity, addressing a significant public health concern, particularly in low-to-middle-income countries where over 76.6% of milk samples have compromised quality and 77.89% are adulterated.

Discovery of antitubercular potential of trans-3-indoleacrylic acid and its derivatives targeting Mycobacterium tuberculosis: A combined in vitro and in silico investigation.

The persistent global health challenges posed by Mycobacterium tuberculosis (Mtb) underscore the urgent need to identify novel antitubercular agents from natural origin. The objective of this research is to assess the antitubercular potential of phytocompounds from Datura innoxia against Mtb through in vitro and in silico methods. Eleven natural compounds were screened for antitubercular activity.

Unravelling anti-cancer properties of solanaceous extracts using GC-MS and HPLC.

Cancer is a complex health issue that today's medical science is dealing with, which has a mortality rate that is almost identical to that of cardiac disorders. Because of the adverse effects of the majority of the medications used in its therapy, managing it remains a major concern despite the availability of numerous remedies. This article attempts to contribute to the scientific developments in the Solanaceae family's anti-cancer field.

TRAIL mediated apoptosis ruling and anticancer trigger by fine-tuned nano spheres of Fagonia cretica methanolic extracts as novel cancer regime.

Fagonia cretica L. is a tropical plant of family Zygophyllaceae with wide range of medicinally important secondary metabolites. The low cellular uptake of the polar compounds in the extract of the plant limits its biological application.

Exogenous Application of Green Titanium Dioxide Nanoparticles (TiO NPs) to Improve the Germination, Physiochemical, and Yield Parameters of Wheat Plants under Salinity Stress.

Agriculture is the backbone of every developing country. Among various crops, wheat ( L.) belongs to the family Poaceae and is the most important staple food crop of various countries.

Designing of pretreatment filter technique for reduction of phenolic constituents from olive-mill wastewater and testing its impact on wheat germination.

Olive oil extraction produces a great volume of olive mill wastewater (OMW), which is considered a serious ecological challenge. In this study, we have designed and tested a trickling filter consisting of seven different layers of natural material, including (coarse gravel, fine gravel, lime (Ca (OH)), sand (SiO), carbon char, sponge/mesh), to treat OMW. The filter process involved physical separation, filtration, coagulation and adsorption with the removal of COD (69.

Natural plant extracts mediated expression regulation of TGF-β receptors and SMAD genes in human cancer cell lines.

Background: Transforming growth factor beta (TGF-β) superfamily has key role in cell proliferation which leads to tumor promoting activities at metastatic stage of cancer. Inhibition of transforming growth factor beta receptor (TGFβR) signaling pathway can provide better therapeutic strategy to control cancer. Natural products are best known for their safety, less toxic nature, antioxidant characteristics making them a promising candidate to inhibit TGFβR signaling pathway.

Nutraceutical profiling of elite onion germplasm and breeding hybrids with improved nutraceutical quality.

Onion (Allium cepa L) is a major reservoir of important nutraceutical ingredients. Herein, nutraceutical profiling of elite germplasm was assessed and hybrids with improved nutraceutical quality were selected. The nutraceutical components were screened through Fourier Transform Infrared Spectroscopy (FTIR) analysis (scan range 4000-400cm-1) followed by spectrophotometric/colorimetric quantification in oven dried bulb samples.

Biostimulation potential of biochar for remediating the crude oil contaminated soil and plant growth.

Crude oil contamination is a serious environmental threat to soil and plants growing in it. Biochar has the potential of biostimulation for remediation of crude oil-contaminated soil. Therefore, the current research was designed to analyze the bio-stimulatory impact of biochar for remediating the crude oil contaminated soil (10%, and 15%), and growth of maize under glasshouse conditions.

Correlation of C-reactive protein levels, gene polymorphism and platelets count in Dengue infection.

Objective: To determine the correlation of polymorphism in C-reactive protein gene with variation in serum levels in dengue patients.

Methods: The cross-sectional study was conducted at Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan, from October 2017 to October 2018, and comprised blood samples from dengue patients which were used to measure the serum levels of C-reactive protein. Deoxyribonucleic acid extraction followed by tetra amplification-refractory mutation system polymerase chain reaction was used to analyse the genotype variation T>G for single nucleotide polymorphism rs199953854 using allele-specific primers.

Titanium dioxide nanoparticles elicited agro-morphological and physicochemical modifications in wheat plants to control Bipolaris sorokiniana.

The current study involves the biogenesis of titanium dioxide nanoparticles (TiO2 NPs) by using Moringa oleifera Lam. aqueous leaf extract for the reduction of titanium dioxide salt into TiO2 nanoparticles. The biosynthesized TiO2 nanoparticles were observed by using the UV-visible spectrophotometry, SEM, EDX and XRD analytical methods.

Isolation of Oxyberberine and -Sitosterol from Royle Root Bark Extract and Cytotoxicity against Liver and Lung Cancer Cell Lines.

Royle has been traditionally used to cure rheumatism, eye and ear diseases, malarial fever, diabetes, stomach disorders, and skin diseases. There is a least amount of data available on cytotoxic capacity of from Pakistani origin, so on this basis, the present study was aimed to screen root bark extracts for cytotoxicity against cancer cell lines and isolation of chemical constituents from the most cytotoxic extract. Initial screening of extracts was performed on HepG2 cells at 100 g/mL for 72 hours of treatment by using an MTT assay.

Optimization of soya lecithin and Tween 80 based novel vitamin D nanoemulsions prepared by ultrasonication using response surface methodology.

Vitamin D nanoemulsions were fabricated using ultrasonic homogenization approach. Response surface methodology (RSM) was used to optimize the preparation conditions for mixed surfactants (Soya lecithin and Tween 80; 2:3) based nanoemulsions. The effects of homogenization time (3.

Bioactivity evaluation and HPLC UV-VIS based quantification of antioxidant secondary metabolites from extract and fractions of Bistorta amplexicaulis rhizome.

Bistorta amplexicaulis is a popular medicinal plant and reported as rich source of antioxidant compounds. The present study was designed for antioxidant and anticancer potential of polarity based fractions of B. amplexicaulis and its correlation to the secondary metabolites quantified by HPLC-UV/VIS.

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress.

This study was organised to check the effect of silver nanoparticles and silver nitrate on rice growth against biotic stress. Silver nanoparticles were synthesised by using plant extract as reducing agent, followed by characterisation through UV Vis spectroscopy, XRD, EDS and SEM. Aspergillus application significantly reduced rice plant fresh mass (0.

Optimization of mixed surfactants-based β-carotene nanoemulsions using response surface methodology: An ultrasonic homogenization approach.

In the present study, food grade mixed surfactant-based β-carotene nanoemulsions were prepared without using any co-surfactant. Response surface methodology (RSM) along with central composite design (CCD) was used to investigate the effect of independent variables (surfactant concentration, ultrasonic homogenization time and oil content) on response variables. RSM analysis results revealed that experimental results were best fitted into a quadratic polynomial model with regression coefficient values of more than 0.

Knowledge, attitude and practices (KAP) of the families of b-thalassaemia children in thalassaemia centers of Rawalpindi and Islamabad, Pakistan.

The present study was designed to assess the Knowledge, Attitude and Practices (KAP) of the parents of b-thalassaemia children (410) selected from public (73.2%) and private (26.8%) thalassaemia centers of Rawalpindi-Islamabad.

Natural Agents-Mediated Targeting of Histone Deacetylases.

In the past few years, basic and clinical scientists have witnessed landmark achievements in many research projects, such as those conducted by the US National Institutes of Health Roadmap Epigenomics Mapping Consortium, the International Human Epigenome Consortium, The Cancer Genome Atlas Network and the International Cancer Genome Consortium, which have provided near-complete resolution of epigenetic landscape in different diseases. Furthermore, genome sequencing of tumors has provided compelling evidence related to frequent existence of mutations in readers, erasers and writers of epigenome in different cancers. Histone acetylation is an intricate mechanism modulated by two opposing sets of enzymes and deeply studied as a key biological phenomenon in 1964 by Vincent Allfrey and colleagues.

The In Vitro and In Vivo Biological Activities of the Leaf of Cape Myrtle, Myrsine africana L.

The cape myrtle, Myrsine africana L., is a widely used medicinal plant, which has not been well investigated. We assessed the in vivo hepatoprotective and in vitro antiproliferative and antioxidant effects of leaf extracts of M.

Effect of leaf extracts of Taraxacum officinale on CCl4 induced hepatotoxicity in rats, in vivo study.

Taraxacum officinale L is a medicinal plant, which has enormous medicinal values against various types of liver disorders and it has traditionally been used for the treatment of liver problems by people from the South East Asia. Previously we have screened the crude methanolic extract of T. officinale against cytotoxicity induced by CCl4.

Antioxidant and Cytotoxic Activities and Phytochemical Analysis of Euphorbia wallichii Root Extract and its Fractions.

Euphorbia wallichii a perennial herb growing mainly in Himalayas has been widely used in folk medicines for its medicinal properties. In the present study, the crude methanolic root extract (CME) and its fractions; n-Hexane Fraction (NHF), n-Butanol Fraction (NBF), Chloroform Fraction (CHF), Ethyl acetate Fraction (EAF) and Aqueous Fraction (AQF) of this plant specie were investigated for antioxidant and cytotoxic activities and phytochemical analysis. Antioxidant activity was determined by using 2,2-diphenyl-1-picryl-hydrazyl free radical (DPPH) and DNA protection assay performed on pBR322 plasmid DNA.

Structure elucidation and inhibitory effects of self-assembled organotin(IV) esters of p-tolyl acetic acid on bacterial, fungal, brine shrimp, and potato tumor cells.

Biologically active new organotin(IV) acetates (1-6) of p-tolyl acetic acid have been synthesized and characterized by multinuclear nuclear magnetic resonance ((1)H, (13)C) and mass spectrometry. The X-ray crystallographic data of compounds (3 and 4) were also collected. These studies show that in diorganotin(IV) compounds (compounds 1, 2, 3, and 6), tin exists in skew trapezoidal geometry with four strong and two weaker bonds, whereas triorganotin(IV) carboxylates (compounds 4 and 5) have four and five coordinated geometries in solution and solid form, respectively.