Detection of pathogens in dogs with respiratory disease during winter 2023-2024 using multiplex qPCR/RT-qPCR assays and next-generation sequencing.

Canine Infectious Respiratory Disease Complex (CIRDC), caused by a diverse range of viral and bacterial pathogens, is the leading cause of respiratory illness in dogs. In the winter of 2023-2024, the United States experienced a noticeable increase in cases consistent with CIRDC. This study investigated the potential association of emerging pathogens with CIRDC cases.

Bone health in cancer: perspective on the use of osteoanabolic agents.

Cancer negatively impacts bone health through various mechanisms, including treatment-induced bone loss and malignant bone lesions, often leading to increased fracture risk and higher morbidity and mortality. Antiresorptive agents (bisphosphonates and denosumab) are the current standard of care to reduce the risk of skeletal-related events and prevent treatment-related fragility fractures in patients with cancer. While there is strong evidence to support their benefits in cancer, there is potential room for further reduction in the risk of pathologic and fragility fractures.

Associations of urinary zinc with incident peripheral artery disease and amputation in the Strong Heart Study.

Background And Aims: Urinary zinc has been associated with cardiometabolic endpoints, including diabetes and cardiovascular disease, but evidence for peripheral artery disease (PAD) is limited. We evaluated prospective associations between urinary zinc and incident PAD and amputation events in the Strong Heart Study (SHS), a large epidemiological cohort of American Indian adults in the United States.

Methods: A total of 2,045 PAD-free and 2,180 amputation-free participants were included at baseline (1989-91), (mean age 56 years, 61% female).

Equine Sarcoid Arising From the Medial Caruncle With Invasion of the Third Eyelid in a Quarter Horse Gelding.

Objective: To describe the clinical and histologic features of a sarcoid arising from the medial caruncle with spread to the third eyelid of a horse.

Animal Studied: A 20-year-old Quarter Horse gelding.

Procedures: An incisional biopsy of the medial caruncle mass was initially performed, with histopathology suggesting a sarcoid or other spindle cell neoplasm.

Training of physical neural networks.

Physical neural networks (PNNs) are a class of neural-like networks that make use of analogue physical systems to perform computations. Although at present confined to small-scale laboratory demonstrations, PNNs could one day transform how artificial intelligence (AI) calculations are performed. Could we train AI models many orders of magnitude larger than present ones? Could we perform model inference locally and privately on edge devices? Research over the past few years has shown that the answer to these questions is probably "yes, with enough research".

Developing Validity Evidence for a Novel Pediatric Telemedicine Assessment Tool (PTAT) for Pediatric Residents: A Pilot Study.

Objectives: Telemedicine use among pediatric residents has grown in recent years, accompanied by the development of best practices and curricula that outline essential telemedicine skills. However, there is a notable lack of methods to assess these skills. Our work aims to address this gap by designing and building validity evidence for a novel pediatric telemedicine assessment tool, the Pediatric Telemedicine Assessment Tool (PTAT).

Diagnosis and treatment of inguinal midline abdominal wall herniation of the urinary bladder in three domestic rabbits (Oryctolagus cuniculus).

Inguinal herniation of the urinary bladder through the inguinal rings of domestic rabbits (Oryctolagus cuniculus) has been well-described in veterinary literature due to the inguinal rings remaining open throughout the lifespan of adult males. Midline body wall herniation has also been described in several veterinary species due to a variety of etiologies, including trauma, incisional dehiscence, and congenital anomalies. This phenomenon has not previously been described in rabbits.

Preoperative Testosterone Replacement Therapy is Associated with Increased Rates of PJI and Medical Complications after Total Shoulder Arthroplasty: A Propensity-Score Matched Analysis.

Background: The use of testosterone replacement therapy (TRT) has increased in recent years, however, its effect on surgical outcomes and long-term implant survival in total shoulder arthroplasty (TSA) remains unclear. This study aimed to assess the association between preoperative TRT and postoperative complications following TSA.

Methods: The TriNetX database was queried to identify patients undergoing TSA before 2020.

Rare variants in PRKCI cause Van der Woude syndrome and other features of peridermopathy.

Van der Woude syndrome (VWS) is an autosomal dominant disorder characterized by lower lip pits and orofacial clefts (OFCs). With a prevalence of ∼1 in 35,000 live births, it is the most common form of syndromic clefting. Most VWS is attributed to variants in IRF6 (∼70%) or GRHL3 (∼5%), leaving up to 25% of individuals without a molecular diagnosis.

Serogroup W invasive meningococcal disease outbreak in Qatar following travel to Umrah.

Invasive meningococcal disease (IMD) is associated with significant morbidity and mortality in all age groups. Globally, reported cases predominantly belong to serogroups A, B, and C. Although serogroup W is less prevalent with regional distribution, it demonstrates shifting epidemiology linked to travel.

Chemical Choreography at the Mn Oxide Interface: Protein Association Modulates Glyphosate Bonding Configurations and Favors the AMPA Oxidation Pathway.

Glyphosate, the most widely used herbicide, undergoes adsorption and abiotic degradation on environmental surfaces, with natural organic matter strongly influencing these processes. The role of organo-mineral associations in regulating glyphosate retention and transformation pathways, however, remains unclear. In this study, we employed time-resolved ATR-FTIR spectroscopy and microfluidic experiments coupled with LC-MS quantification to track glyphosate and its oxidation byproducts.

aspartyl protease 2 is required for host cell egress of merozoites and male gametes.

The parasite causes severe diarrheal disease that can be life-threatening, and effective treatments are sorely lacking. Recently, aspartyl proteases (ASP) have emerged as targets with significant therapeutic potential in several related parasites resulting in the development of multiple potent leads. ASPs are critical to the proteolytic activation and maturation of secretory proteins parasites rely on to invade, manipulate, and upon completion of their replication cycle exit the host cells in which they reside.

Block Grant Terminations at the Substance Abuse and Mental Health Services Administration in Early 2025: A Descriptive Analysis.

Objective: This study aimed to quantify and characterize the scope of SAMHSA grant terminations in early 2025 following a federal leadership change and departmental restructuring, with a focus on funding sources, recipient types, and geographic distribution.

Methods: Terminated grants from the Substance Abuse and Mental Health Services Administration (SAMHSA) were identified using the Tracking Accountability in Government Grants System (TAGGS) and cross-referenced with USAspending.gov.

Methotrexate-Associated Primary Cutaneous Epstein-Barr Virus-Positive Polymorphic B-Cell Lymphoproliferative Disorder With Metachronous T-Cell Clonally Related Angioimmunoblastic T-Cell Lymphoma: A Case Report and Review of the Literature.

Primary cutaneous Epstein-Barr virus (EBV)-positive polymorphic B-cell lymphoproliferative disorder (LPD) is a rare LPD associated with iatrogenic and endogenous immune dysregulation with the commonest risk factor being immunosuppressive therapy. We present a 55-year-old woman with rheumatoid arthritis, previously on methotrexate, who developed a waxing and waning papulonodular eruption on the chest and neck. Histopathology revealed a lymphohistiocytic infiltrate with atypical EBV+/CD30+ B cells, consistent with EBV+ polymorphic B-cell LPD.

Distribution of viral hemorrhagic septicemia virus in the nearshore fish community of the St. Lawrence River.

Objective: We investigated viral hemorrhagic septicemia virus dynamics in wild fish populations of the St. Lawrence River to compare host competency among five abundant native species and the invasive Round Goby Neogobius melanostomus following two decades of host-pathogen coevolution. To examine species' responses to the virus and identify principal hosts as viral reservoir species, we used viral surveillance among Round Gobies, Yellow Perch Perca flavescens, Rock Bass Ambloplites rupestris, Pumpkinseed Lepomis gibbosus, Bluegill L.

Hurricane Exposure and Risk of Long-Term Cardiovascular Disease Outcomes.

Importance: Hurricanes are associated with increased cardiovascular disease (CVD) risk, yet little is known about whether these risks extend into the long term and for how long.

Objective: To examine the association between hurricane-related flooding and CVD risk up to 5 years after landfall.

Design, Setting, And Participants: This cohort study included a 20% national sample of continuously enrolled Medicare fee-for-service beneficiaries from New Jersey, New York City, and Connecticut from January 1, 2010, to December 31, 2017.

Multi-Institutional Study of ALK-Positive Large B-Cell Lymphoma: Outcomes in the Era of ALK Inhibitors and Biologically Informed Therapies.

Anaplastic lymphoma kinase-positive large B-cell lymphoma (ALK+ LBCL) is a rare, aggressive subtype of diffuse large B-cell lymphoma with poor outcomes using standard chemotherapy. In this multi-institutional retrospective study, we analyzed 39 cases of ALK+ LBCL identified at six US academic centers from 2002 to 2024, with treatment including conventional cytotoxic regimens in frontline and biologically informed and nonchemotherapy-based strategies in the relapsed setting. Ninety-two percent of patients received frontline anthracycline-based chemotherapy; 43% received intensified regimens, and 15% underwent upfront autologous stem cell transplantation (ASCT).

Relationship Between Paralumbar Muscle Assessment Methods and Spinal Alignment in Adult Spinal Deformity.

Study designRetrospective cross-sectional study.ObjectivesParalumbar muscle degeneration is closely linked to adult spinal deformity (ASD). However, assessment methods vary widely, including quality via fat infiltration (FI) and quantity through paraspinal total cross-sectional area (PTCSA), psoas vertebral body index (PVBI), and normalized total psoas area (NTPA).

Biofluorescence as a tool to resolve crypsis in two bonefish species, Albula goreensis and Albula vulpes, with comments on other external morphology.

Bonefish Albula spp. in the Western Atlantic have historically been morphometrically and phenotypically indiscernible. We used biofluorescence in two sympatric, cryptic bonefish species, Albula goreensis and Albula vulpes, to develop a rapid and inexpensive in situ survey for species identification that can be used by managers and recreational anglers.

Phosphorus constrains global photosynthesis more than nitrogen does.

Global vegetation growth is thought to be limited by nitrogen (N) more than by other nutrients. Here we document a stronger phosphorus (P) limitation on global photosynthesis compared with N over the last four decades. On the basis of more than 80,000 field observations of foliar nutrients and a machine learning method, we generated a long-term global dataset of foliar N and P concentrations for the period 1980-2017.

Recommendations for disclosure of artificial intelligence in scientific writing and publishing: a regional anesthesia and pain medicine modified Delphi study.

Introduction: The use of artificial intelligence (AI) in the scientific process is advancing at a remarkable speed, thanks to continued innovations in large language models. While AI provides widespread benefits, including editing for fluency and clarity, it also has drawbacks, including fabricated content, perpetuation of bias, and lack of accountability. The editorial board of (RAPM) therefore sought to develop best practices for AI usage and disclosure.