98%
921
2 minutes
20
Non-invasive micro-test technology (NMT) reveals dynamic ionic/molecular concentration gradients by measuring fluxes of ions and small molecules in liquid media in 1D, 2D or 3D fashions with sensitivity up to pico- (10) or femto- (10) moles per cm per second. NMT has been applied to study metabolism, signal transduction, genes and/or proteins physiological functions related to transmembrane ionic/molecular activities with live samples under normal conditions or stress. Data on ion and/or molecule homeostasis (IMH) by NMT in biomedical sciences, plant and crop sciences, environmental sciences, marine and space biology as well as traditional Chinese medicine are reviewed.
Download full-text PDF |
Source |
---|---|
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035745 | PMC |
http://dx.doi.org/10.52601/bpr.2024.240009 | DOI Listing |
Biochem Biophys Res Commun
August 2025
Ministry of Education Key Laboratory of Cell Activities and Stress Adaptations, School of Life Sciences, Lanzhou University, Lanzhou, 730000, China; Key Laboratory of Gene Editing for Breeding, School of Life Sciences, Lanzhou University, Lanzhou, Gansu, 730000, China. Electronic address: xiaochb@lz
Ammonium (NH) toxicity significantly limits nitrogen use efficiency (NUE) in agriculture. Nitrate (NO) supplementation mitigates this toxicity, with the anion channel SLAH3 playing a central role by mediating NO efflux to counteract NH-induced rhizosphere acidification. SLAH3, a plasma membrane protein with ten transmembrane domains and cytosolic N- and C-termini, is intrinsically silent.
View Article and Find Full Text PDFPlant Physiol
August 2025
National Key laboratory for tea Plant Germplasm innovation and Resource Utilization, Anhui Agricultural University, 230036 Hefei, Anhui, P R China.
Plants employ sophisticated volatile-mediated signaling mechanisms to defend against herbivore attacks; however, the molecular pathways underlying these processes remain poorly understood. Herein, we unveil the molecular mechanism by which the herbivore-induced volatile (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT) activates jasmonic acid (JA) biosynthesis in tea plants (Camellia sinensis). We demonstrate that DMNT triggers early signaling events in tea plants, characterized by a rapid influx of Ca2+ in mesophyll cells, which subsequently initiates a signaling cascade involving the CsCAMTA3 (calmodulin-binding transcription activator, CAMTA).
View Article and Find Full Text PDFJ Hazard Mater
August 2025
Key Laboratory for Subtropical Wetland Ecosystem Research of MOE, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian 361005, China. Electronic address:
Cadmium (Cd), as a widely investigated heavy metal, demonstrates severe toxicity to plant growth and development. However, the roles of energy metabolism, and protein degradation caused by autophagy in plant Cd tolerance remain poorly characterized. This study reveals that Cd-exposure simultaneously affects both the glycolytic and autophagic in Arabidopsis thaliana.
View Article and Find Full Text PDFPlants (Basel)
June 2025
College of Biology and Oceanography, Weifang University, Weifang 261061, China.
Non-invasive Micro-test Technology (NMT) represents a pioneering approach in the study of physiological functions within living organisms. This technology possesses the remarkable capability to monitor the flow rates and three-dimensional movement directions of ions or molecules as they traverse the boundaries of living organisms without sample destruction. The advantages of NMT are multifaceted, encompassing real-time, non-invasive assessment, a wide array of detection indicators, and compatibility with diverse sample types.
View Article and Find Full Text PDF