The MdGLK1-MdBZR1 Module Integrates Ethylene and Strigolactone Signals to Regulate Cold Tolerance via a CBF-Dependent Pathway in Apple.

Although ethylene and strigolactone (SL) are key regulators of cold tolerance in plants, the molecular crosstalk between their signalling pathways is poorly understood. Here, we identified the transcription factor GOLDEN2-LIKE1 (MdGLK1) as a central integrator of ethylene and SL signalling during the apple (Malus × domestica) cold stress response. MdGLK1 enhanced cold tolerance by recruiting BRASSINAZOLE-RESISTANT1 (MdBZR1), a core component of brassinosteroid signalling, thereby promoting MdBZR1-mediated transcriptional activation of the cold-responsive genes C-REPEAT BINDING FACTOR1 (MdCBF1) and MdCBF2.

Non-Invasive Micro-Test Technology in Plant Physiology Under Abiotic Stress: From Mechanism to Application.

Non-invasive Micro-test Technology (NMT) represents a pioneering approach in the study of physiological functions within living organisms. This technology possesses the remarkable capability to monitor the flow rates and three-dimensional movement directions of ions or molecules as they traverse the boundaries of living organisms without sample destruction. The advantages of NMT are multifaceted, encompassing real-time, non-invasive assessment, a wide array of detection indicators, and compatibility with diverse sample types.

Generation of High-Brilliance Polarized γ-Rays Via Vacuum Dichroism-Assisted Vacuum Birefringence.

High-brilliance circularly polarized γ-photon beams are of great significance for a wide range of applications. However, their generation through nonlinear Compton scattering must require a high-density longitudinally-spin-polarized electron beam and consequently is still a great challenge. Here, a novel method is proposed to generate such γ-photon beams via the vacuum dichroism (VD)-assisted vacuum birefringence (VB) effect, only utilizing a well-established unpolarized electron beam.

Efficient Expression of an Engineered Heparan Sulfate 2--Sulfotransferase with Improved Catalytic Properties.

Heparin, as a type of highly sulfated polysaccharide, is crucial for various physiological and pathophysiological functions. Heparan sulfate 2--sulfotransferase (2ST) is responsible for the second sequential sulfation modification in heparin biosynthesis. However, challenges such as low expression and poor enzyme activity performance limit its application.

Involvement of the miR156/SPLs/NLP7 modules in plant lateral root development and nitrogen uptake.

This study unravels a molecular mechanism of miR156/SPL module in regulating lateral root development and nitrogen uptake in apple. Nitrogen is critical in controlling lateral root development such as the availability or lack of nitrogen nutrients which can affect lateral root formation. The miR156/SPL module plays a pivotal role in regulating many aspects of plant development, including the timing of vegetative phase change, floral induction, shoot branching and root development.

MhIDA small peptides modulate the growth and development of roots in Malus hupehensis.

MhIDA small peptides promote apple root growth by enhancing auxin synthesis and cell wall remodeling gene expression, revealing a peptide-based strategy to improve root architecture. Although small peptides have been well documented as crucial regulators of plant growth and development, the molecular mechanisms underlying lateral root morphogenesis in Malus hupehensis remain poorly understood. In this research, exogenous application of 1 µM MhIDA-Like family peptides increased primary root (PR) length by 14.

Apple MIEL1/ABI5-MAX2 regulatory module links strigolactone and abscisic acid signals.

In apple (Malus domestica), the abscisic acid (ABA)-responsive factor ABA INSENSITIVE5 directly activates MORE AXILLARY GROWTH2 (MdMAX2), an important strigolactone signaling component; an abscisic acid-restricted E3 ubiquitin ligase modulates MdMAX2 turnover, thus linking strigolactone and abscisic acid signaling.

Heterologous expression of a highly thermostable L-asparaginase from Thermococcus zilligii in Aspergillus niger for efficient reduction of acrylamide in French fries.

L-asparaginase (L-ASNase) can hydrolyze L-asparagine, a precursor to acrylamide, thereby reducing toxic acrylamide formation in fried foods. Currently, commercial L-ASNases are primarily produced by wild-type (WT) filamentous fungi; however, these enzymes often exhibit rapid activity loss during high-temperature processing due to limited thermal stability. In this study, we screened a thermostable L-ASNase gene from thermophile bacteria and expressed it in Aspergillus niger to reduce acrylamide content in French fries.

Role of -Glycosylation in Gastrointestinal Cancers.

Gastrointestinal cancers pose a significant global health challenge. -glycosylation modulates various cellular processes, including key cancer-related mechanisms. Elucidating its involvement in the onset and advancement of these cancers can offer critical insights for enhancing diagnostic and therapeutic approaches.

[Heterologous expression of bacterial tyrosinase and its applications in biological hair dyeing and DOPA modification of hydrolyzed silk fibroin].

Tyrosinase is a copper-containing polyphenol oxidase widely applied in the food, cosmetics, pharmaceutical, and other industries. Currently, the production of commercial tyrosinase primarily relies on extraction from fungi, which has high costs, low purity, low specific activity, and poor stability. The objective of this study is to obtain highly expressed bacterial tyrosinase with potential for industrial applications.

Immobilized high-performance heparin lyase III for efficient preparation of low molecular weight heparin.

Heparin lyase III has garnered widespread attention due to its high specificity and minimal loss of anticoagulant activity during the preparation of low molecular weight heparin (LMWH), a crucial anticoagulant drug in clinical practice. However, low expression levels and complex preparation processes limit its practical application. To address these challenges, high-performance Bacteroides thetaiotaomicron heparin lyase III (Bhep III) variants were engineered and immobilized for LMWH preparation.

Engineering artificial cross-species promoters with different transcriptional strengths.

As a fundamental tool in synthetic biology, promoters are pivotal in regulating gene expression, enabling precise genetic control and spurring innovation across diverse biotechnological applications. However, most advances in engineered genetic systems rely on host-specific regulation of the genetic portion. With the burgeoning diversity of synthetic biology chassis cells, there emerges a pressing necessity to broaden the universal promoter toolkit spectrum, ensuring adaptability across various microbial chassis cells for enhanced applicability and customization in the evolving landscape of synthetic biology.

Microfluidic Chip Coupled with MALDI-TOF MS for Multitarget Detection of Allergens in Crucian Carp ().

The goal of the present study was to establish a rapid, simple method for simultaneous allergy testing of sera from multiple fish-allergic patients. Sera from fish-allergic patients were pooled and used for capturing allergens in fish muscle of crucian carp (), which was studied as a fish model. Sarcoplasmic proteins of crucian carp () were extracted for the analysis of allergens.

The MdNAC72-MdABI5 module acts as an interface integrating jasmonic acid and gibberellin signals and undergoes ubiquitination-dependent degradation regulated by MdSINA2 in apple.

Jasmonic acid (JA) and gibberellin (GA) coordinately regulate plant developmental programs and environmental cue responses. However, the fine regulatory network of the cross-interaction between JA and GA remains largely elusive. In this study, we demonstrate that MdNAC72 together with MdABI5 positively regulates anthocyanin biosynthesis through an exquisite MdNAC72-MdABI5-MdbHLH3 transcriptional cascade in apple.

Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A.

Chondroitin sulfate (CS) stands as a pivotal compound in dietary supplements for osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of environmentally friendly and safe CS production. Enzymatic synthesis of CS for instance CSA has been considered as one of the most promising methods. However, the bottleneck consistently encountered is the active expression of chondroitin 4-O-sulfotransferase (C4ST) during CSA biosynthesis.

Enzymatic construction of a library of even- and odd-numbered heparosan oligosaccharides and their N-sulfonated derivatives.

Low-molecular-weight heparins (LMWHs), especially the specific-sized heparin oligosaccharides, are attractive for the therapeutic applications, while their synthesis remains challenging. In the present study, unsaturated even-numbered heparosan oligosaccharides were firstly prepared by cleaving high-molecular-weight heparosan using recombinant heparinase III (HepIII). The conversion rates of the unsaturated disaccharides, tetrasaccharides, hexasaccharides, octasaccharides, and decasaccharides were 33.

MdbHLH162 connects the gibberellin and jasmonic acid signals to regulate anthocyanin biosynthesis in apple.

Anthocyanins are secondary metabolites induced by environmental stimuli and developmental signals. The positive regulators of anthocyanin biosynthesis have been reported, whereas the anthocyanin repressors have been neglected. Although the signal transduction pathways of gibberellin (GA) and jasmonic acid (JA) and their regulation of anthocyanin biosynthesis have been investigated, the cross-talk between GA and JA and the antagonistic mechanism of regulating anthocyanin biosynthesis remain to be investigated.

Manipulation of Giant Multipole Resonances via Vortex γ Photons.

Traditional photonuclear reactions primarily excite giant dipole resonances, making the measurement of isovector giant resonances with higher multipolarities a great challenge. In this Letter, the manipulation of collective excitations of different multipole transitions in even-even nuclei via vortex γ photons is investigated. We develop the calculation method for photonuclear cross sections induced by the vortex γ photon beam using the fully self-consistent random-phase approximation plus particle-vibration coupling (RPA+PVC) model based on Skyrme density functional.

Engineering sulfonate group donor regeneration systems to boost biosynthesis of sulfated compounds.

Sulfonation as one of the most important modification reactions in nature is essential for many biological macromolecules to function. Development of green sulfonate group donor regeneration systems to efficiently sulfonate compounds of interest is always attractive. Here, we design and engineer two different sulfonate group donor regeneration systems to boost the biosynthesis of sulfated compounds.

Production of different molecular weight glycosaminoglycans with microbial cell factories.

Glycosaminoglycans (GAGs) are naturally occurring acidic polysaccharides with wide applications in pharmaceuticals, cosmetics, and health foods. The diverse biological activities and physiological functions of GAGs are closely associated with their molecular weights and sulfation patterns. Except for the non-sulfated hyaluronan which can be synthesized naturally by group A Streptococcus, all the other GAGs such as heparin and chondroitin sulfate are mainly acquired from animal tissues.

Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides.

Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical physiological functions. CS is widely applied in medical and clinical fields. Currently, the supply of CS relies on traditional animal tissue extraction methods.

Exploring the proteins and metabolites associated with male antennae responses to female exposure of Antheraea pernyi (Lepidoptera: Saturniidae) moths.

Detection of sex pheromones of insects relies on the antennae. The female pheromone signal transmission in the male antennae ultimately initiates the courtship and mating behaviors of males. To investigate the proteins and metabolites involved in this neural transduction, integrative proteomics and metabolomics analysis including tandem mass tag (TMT) proteomic quantification and liquid chromatography tandem mass spectrometry (LC/MS)-based metabolomics was adopted for comparing proteomic and metabolic changes between the antennae of male moths following stimulation by females and the non-stimulated males of Antheraea pernyi (Guérin-Méneville, Lepidoptera: Saturniidae) in this study.

The Spliceosome Factor EFTUD2 Promotes IFN Anti-HBV Effect through mRNA Splicing.

Methods: Using a CRISPR/Cas9 gene-editing system, single allele knockout HepG2.2.15 cells were constructed.