Unveiling the protective role of recombinant snow flea antifreeze protein expressed by Komagataella phaffii in cryopreservation of EA.hy926 cells.

Cryopreservation is pivotal for preserving the viability and functionality of cells, tissues, and organs, which is crucial for medical research and therapeutic applications. In this study, recombinant snow flea antifreeze protein (rSfAFP) was produced using Komagataella phaffii GS115 and evaluated for cryopreservation of human umbilical vein endothelial cells (EA.hy926).

Cognitive Emotion-Regulation and Depressive Symptoms in a Group of Healthcare Workers During the COVID-19 Pandemic: A Person-Based Approach.

This study aimed to identify profiles of cognitive emotion regulation strategies and to examine the association between emotion regulation profiles and depressive symptoms using a sample of healthcare workers under the impact of COVID-19. A total of 3477 Chinese healthcare workers participated in the online survey. Latent profile analysis was used to identify cognitive regulation patterns using nine strategies.

Heterologous expression of a highly thermostable L-asparaginase from Thermococcus zilligii in Aspergillus niger for efficient reduction of acrylamide in French fries.

L-asparaginase (L-ASNase) can hydrolyze L-asparagine, a precursor to acrylamide, thereby reducing toxic acrylamide formation in fried foods. Currently, commercial L-ASNases are primarily produced by wild-type (WT) filamentous fungi; however, these enzymes often exhibit rapid activity loss during high-temperature processing due to limited thermal stability. In this study, we screened a thermostable L-ASNase gene from thermophile bacteria and expressed it in Aspergillus niger to reduce acrylamide content in French fries.

Predicting major adverse cardiac events using radiomics nomogram of pericoronary adipose tissue based on CCTA: A multi-center study.

Background: The evolution of coronary atherosclerotic heart disease (CAD) is intricately linked to alterations in the pericoronary adipose tissue (PCAT). In recent epochs, characteristics of the PCAT have progressively ascended as focal points of research in CAD risk stratification and individualized clinical decision-making. Harnessing radiomic methodologies allows for the meticulous extraction of imaging features from these adipose deposits.

Effect of peracetic acid generation in hydrogen peroxide-acetic acid pretreatment on production of xylooligosaccharides from poplar by organic acid hydrolysis.

Hydrogen peroxide-acetic acid (HPAA) compositions affect the peracetic acid generation and subsequent delignification of lignocellulosic materials. However, the effects of HPAA compositions on lignin removal and poplar hydrolyzability after HPAA pretreatment are not fully elucidated yet. In this work, different volume ratios of HP to AA were used to pretreat poplar, AA and lactic acid (LA) hydrolysis of delignified poplar to produce XOS was compared.

New strategy to elucidate the positive effects of extractable lignin on enzymatic hydrolysis by quartz crystal microbalance with dissipation.

Background: The presence of lignin normally affects enzymatic saccharification of lignocellulose detrimentally. However, positive effects of lignin on enzymatic hydrolysis have been recently reported. Enzyme-lignin interactions could be the key to reveal the underlying mechanism of their discrepant behaviors.