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We present a water-soluble fluorescence/MR dual-modality probe NBD[Gd] for HS detection. It showed 88.4% of fluorescence quenching and a 36% reduction in upon exposure to HS. It is readily uptaken by cells to sense the endogenous HS. Besides providing a bimodal HS imaging probe, our study also confirms the high HS level in CT26 cells and its functional consequence in promoting cell growth.
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http://dx.doi.org/10.1039/d4cc05042h | DOI Listing |
Mol Pharm
September 2025
Center for Orthopedic Surgery, The Third Affiliated Hospital of Southern Medical University, Guangzhou 510630, China.
Myocardial fibrosis, a key pathological feature of hypertensive heart disease (HHD), remains diagnostically challenging due to limited clinical tools. In this study, a FAPI-targeted uptake mechanism previously reported by our group, originally developed for tumor imaging, is extended to the detection of myocardial fibrosis in HHD using [F]F-NOTA-FAPI-MB. The diagnostic performance of this tracer is compared with those of [F]F-FDG, [F]F-FAPI-42, and [F]F-NOTA-FAP2286, and its potential for fluorescence imaging is also evaluated.
View Article and Find Full Text PDFEur J Nucl Med Mol Imaging
August 2025
Department of Nuclear Medicine and Minnan PET Center, Xiamen Key Laboratory of Radiopharmaceuticals, The First Affiliated Hospital of Xiamen University, School of Medicine, Xiamen University, Xiamen, 361003, China.
Purpose: Accurate delineation of tumor margins and maximal safe resection are critical for successful curative oncologic surgery. However, fibroblast activation protein (FAP)-targeted probes suitable for fluorescence imaging remain limited. Here, we developed novel FAP-targeted fluorescent probes to accurately delineate tumor margins and enable rapid intraoperative identification of tumor boundaries in resected specimens.
View Article and Find Full Text PDFBiomed Opt Express
August 2025
School of Medical Engineering and School of Mathematical Medicine, Xinxiang Medical University, Xinxiang 453003, China.
Lymphoma is one of the most common malignancies globally, making early diagnosis crucial for improving survival. This study introduces an electrochemical-assisted scattering imaging system (ESIS) for lymphoma cell classification. The system integrates scattering imaging with electrochemical measurements, using a fiber-optic probe for scattering excitation and a 3D rGO-TiC-MWCNTs composite electrode to simultaneously monitor HO release.
View Article and Find Full Text PDFChem Commun (Camb)
August 2025
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Key Laboratory of Micro Interface Physics and Detection, Chinese Academy of Sciences, Shanghai 201800, China.
In this study, we have developed a dual-mode probe based on click chemistry for spherical nucleic acids (SNAs), achieving extremely specific fluorescence imaging and synchrotron X-ray microscopy of neuronal cells.
View Article and Find Full Text PDFAnal Chem
July 2025
Department of Radiology, Xinhua Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200092, China.
Cancer-associated fibroblasts (CAFs), characterized by the expression of fibroblast activation protein (FAP), play a pivotal role in tumor progression and therapy resistance. FAP-targeted molecular imaging offers a powerful tool for the precise visualization and quantification of CAFs within tumors. In this study, guided by molecular docking analyses, we rationally designed and synthesized two novel FAP-targeted probes, FAPtp1 and FAPtp2, by conjugating FAP-targeted peptides, Mn-NOTA, and indocyanine green (ICG).
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