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In this study, we have developed a dual-mode probe based on click chemistry for spherical nucleic acids (SNAs), achieving extremely specific fluorescence imaging and synchrotron X-ray microscopy of neuronal cells.
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http://dx.doi.org/10.1039/d5cc02645h | DOI Listing |
Colloids Surf B Biointerfaces
September 2025
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei 230026, China; Department of Biomaterials and Stem Cells, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Science (CAS), Suzhou 215163,
Bacterial infections and the associated inflammatory responses present significant challenges to public health, underscoring the need for innovative therapeutic strategies. In this study, novel carbon dots (QA-CDs) derived from quercetin (QU) and 4-aminophenol (4-AP) were synthesized using a one-step hydrothermal method. This approach merges the antimicrobial properties of phenolic compounds with the multifunctional advantages of carbon-based nanomaterials.
View Article and Find Full Text PDFProc Natl Acad Sci U S A
September 2025
Department of Chemistry and International Institute for Nanotechnology, Northwestern University, Evanston, IL 60208.
Genome editing with CRISPR-Cas systems hold promise for treating a wide range of genetic disorders and cancers. However, efficient delivery of genome editors remains challenging due to the requirement for the simultaneous delivery or intracellular generation of Cas proteins, guide RNAs, and, in some applications, donor DNAs. Furthermore, the immunogenicity and toxicity of delivery vehicles can limit the safety and efficacy of genetic medicines.
View Article and Find Full Text PDFNat Chem
September 2025
Department of Chemistry, University of Oxford, Oxford, UK.
The flexible and modular design of synthetic cells, comprising lipid vesicles capable of imitating the structure and function of living cells, facilitates their application as drug delivery devices. The ability to control the synthesis of biomolecules within synthetic cells using a tissue-penetrating stimulus opens up additional levels of functionality that has the potential to improve biological potency and circumvent drug leakage from preloaded vesicles. To this end, we have designed spherical nucleic acids comprising DNA promoter sequences decorating magnetic nanoparticle cores.
View Article and Find Full Text PDFSci Adv
August 2025
China National Center for Bioinformation, Beijing, China.
The measurement of three-dimensional genome folding in the nucleus, mostly through Hi-C methods, is expressed as contact frequencies between genomic segments, without anchoring to physical axes of the spherical nucleus. Here, we mapped the chromatin contacts along nuclear radial axis and built radial score by factoring in contact frequencies. The chromatin high-order structures exhibit rich diversity along radial axis.
View Article and Find Full Text PDFSmall
August 2025
Center for Molecular Imaging and Nuclear Medicine, School of Life Sciences & School for Radiological and Interdisciplinary Sciences (RAD-X) & The Second Affiliated Hospital of Soochow University, Soochow University, Suzhou, 215123, China.
Iron oxide nanoparticles (IONPs) have wide biomedical applications and are initially considered with minimal immunogenicity. Recent studies reveal that IONPs can activate the immune system through reactive oxygen species (ROS) or interferon regulatory factor (IRF) pathways. However, the exact mechanism remains unclear.
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