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Article Abstract
Infectious diseases caused by lead to massive death of fish, compose a serious threat to the global aquaculture industry, and constitute a risk to humans who deal with raw fish. In order to realize the early diagnosis of , and control the outbreak and spread of disease, it is of great significance to establish fast, sensitive, and convenient detection methods for . In the present study, two methods of real-time MIRA (multienzyme isothermal rapid amplification, MIRA) and MIRA-LFD (combining MIRA with lateral flow dipsticks (LFD)) for the gene of were established, which could complete amplification at a constant temperature of 42 °C within 20 min. Real-time MIRA and MIRA-LFD assays showed high sensitivity (97 fg/μL or 7.6 × 10 CFU/mL), which were consistent with the sensitivity of real-time PCR and 10 times higher than that of PCR with strong specificity, repeatability simplicity, and rapidity for originating from . In summary, real-time MIRA and MIRA-LFD provide effective ways for early diagnosis of in aquaculture, especially for units in poor conditions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10178759 | PMC |
| http://dx.doi.org/10.3390/ijms24097733 | DOI Listing |
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