Amyloid β (Aβ) inhibits retinal angiogenesis in Alzheimer's disease via LncRNA-XIST/miRNA-126-5p/VEGF axis.

Alzheimer's disease (AD) is often accompanied by retinal lesions, in which Amyloid β (Aβ) is a key mediator. Vascular dysfunction is always associated with the malignant progression of AD, but the precise mechanisms remain poorly understood. The aim of this study is to investigate how Aβ regulates retinal angiogenesis in AD models through the LncRNA-XIST/miR-126-5p/VEGF axis.

Ultra-Elastic, Transparent, and Conductive Gelatin/Alginate-Based Bioadhesive Hydrogel for Enhanced Human-Machine Interactive Applications.

Growing interest has focused on next-generation flexible adhesive sensors (FAS) integrated with deep learning for intelligent electronics. Existing conductive hydrogels, however, fail to concurrently achieve high stretchability, transparency, robust adhesion, and interactive precision. Here, a novel class of high-performance ionic hydrogels (AGG-M hydrogels, with M being Cu, Zn, Fe, and Zr) that synergistically combines the above features is developed.

[Effect of acupuncture on intracerebral mitochondrial function in mice with cerebral hemorrhage].

Objectives: To observe the effect of acupuncture on the recovery of neurological function in mice with intracerebral hemorrhage (ICH), so as to explore its mechanism of regulating mitochondria in brain tissue of ICH mice through the utilization of RNA sequencing and m6A methylation sequencing technology.

Methods: Twenty-four male C57BL/6J mice were ramondly divided into sham operation, model, and acupuncture groups (=8 in each group). The ICH model was established by injection of the rat's auto-blood (10 μL) into the right caudatum region.

PlantDeepMeth: A Deep Learning Model for Predicting DNA Methylation States in Plants.

Cytosine DNA methylation (5mCs) is an important epigenetic modification in genomic research. However, the methylation states of some cytosine sites are not available due to the limitations of different studies, and there are few tools developed to deal with this problem, especially in plants, which have more methylation types than animals. Here, we report PlantDeepMeth, a novel deep learning model that utilizes deep learning to predict DNA methylation states in plants.

[Mechanisms of acupuncture in inhibition of ferroptosis in intracerebral hemorrhage mice based on RNA m6A methylation sequencing].

Objectives: To observe the effect of acupuncture on N6-methyladenosine (m6A) methylation and ferroptosis in brain tissue of mice with intracerebral hemorrhage (ICH), so as to explore its mechanisms underlying promoting neural function recovery.

Methods: Fifty-four male C57BL/6J mice were randomly divided into sham operation, model and acupuncture groups, with 18 mice in each group. The ICH model was established by injection of the mouse's auto-blood (10 μL) into the right caudate nucleus.

Alteration of Whole Brain Amplitude of Low-Frequency Fluctuations and Fractional Amplitude of Low-Frequency Fluctuations in Patients With Depression After Acceptance and Commitment Therapy: A Resting-State Functional Magnetic Resonance Imaging Study.

Objective: This study aimed to explore the changes in brain functional activity before and after acceptance and commitment therapy (ACT) treatment in patients with major depressive disorder (MDD) and the correlation between brain functional changes and clinical symptoms.

Methods: We recruited 12 patients who met the Diagnostic and Statistical Manual of Mental Disorders, Fifth Edition, criteria for MDD. Patients underwent clinical assessments and resting-state functional magnetic resonance imaging (rs-fMRI) scans before and after ACT intervention.

Sustained release of migrasomes from a methacrylate-oxidized hyaluronic acid/methacrylated gelatin composite hydrogel accelerates skin wound healing.

Migrasomes are newly discovered organelles with diverse physiological and pathological functions. Recent studies have shown that the local injection of fibroblast-derived migrasomes can accelerate skin wound healing; however, their effects are short-lived. To extend their therapeutic effects, we developed a skin-mimicking hydrogel (OHG) composed of methacrylate-oxidized hyaluronic acid (O-HA-MA) and methacrylated gelatin (GelMA).

Functional connectivity gradients and neurotransmitter maps among patients with mild cognitive impairment and depression symptoms.

Background: Both depressive symptoms and neurotransmitter changes affect the characteristics of functional brain networks in clinical patients. We sought to explore how brain functional grading is organized among patients with mild cognitive impairment and depressive symptoms (D-MCI) and whether changes in brain organization are related to neurotransmitter distribution.

Methods: Using 3 T magnetic resonance imaging (MRI) we acquired functional MRI (fMRI) data from patients with D-MCI, patients with mild cognitive impairment without depression (nD-MCI), and healthy controls.

Fractional amplitude of low-frequency fluctuations in right dorsal cingulum bundle associated with depression symptoms in AD patients: effects of donepezil intervention.

Objectives: Our aim was to investigate the mechanisms of spontaneous brain activity of white matter functional signals in Alzheimer's disease (AD) patients after donepezil intervention.

Methods: We used resting-state functional magnetic resonance imaging and the fractional amplitude of low-frequency fluctuations (fALFF) approach to investigate changes in spontaneous brain activity of white matter functional signals in AD patients before and after donepezil intervention. A total of 32 subjects participated in the study, including 16 healthy subjects (HCs) and 16 AD patients.

Fishing net-inspired PVA-chitosan-CNT hydrogels with high stretchability, sensitivity, and environmentally stability for textile strain sensors.

Soft electronic products are being extensively investigated in diverse applications including sensors and devices, due to their superior softness, responsiveness, and biocompatibility. One-dimensional (1-D) fiber electronic devices are recognized for their lightweight, wearable, and stretchable qualities, thus emerging as critical constituents for seamless integration with the human body and attire, exhibiting great potential in wearable applications. However, wearable conductive hydrogel fibers usually face challenges in combining stretchability and excellent stability, notably in high-temperature environment.

Bioprinted, spatially defined breast tumor microenvironment models of intratumoral heterogeneity and drug resistance.

Cellular, extracellular matrix (ECM), and spatial heterogeneity of tumor microenvironments (TMEs) regulate disease progression and treatment efficacy. Developing in vitro models that recapitulate the TME promises to accelerate studies of tumor biology and identify new targets for therapy. Here, we used extrusion-based, multi-nozzle 3D bioprinting to spatially pattern triple-negative MDA-MB-231 breast cancer cells, endothelial cells (ECs), and human mammary cancer-associated fibroblasts (HMCAFs) with biomimetic ECM inks.

Naturally derived double-network hydrogels with application as flexible adhesive sensors.

Hydrogels have been widely used in the biomedical field, including wearable sensors and biological adhesives. However, achieving a balance between various functionalities, such as wet adhesion, stable conductivity, and biocompatibility, in one customized hydrogel has been a challenging issue. In this study, we developed a multifunctional hydrogel comprising recombinant human collagen (RHC) and aldehyde-modified sodium alginate (Ald-alginate), which was primarily crosslinked through a Schiff-base reaction and metal chelation.

The N545S and K717N substitution at the N-glycosylation sites of the S2 subunit of avian infectious bronchitis virus can significantly enhance viral pathogenicity.

The S2 subunit of infectious bronchitis virus (IBV) is a heavily glycosylated protein that can impact various characteristics of the virus. It is currently known that N-glycosylation modifications are predominantly located on the S2 subunit. However, the exact role of their N-glycosylation modification remains undisclosed.

Abnormal white matter integrity of anterior-inferior hypothalamus in mild cognitive impairment patients with depression symptoms.

Background: The hypothalamus is a key brain structure involved in the pathogenesis of depression, and its abnormal activity is considered an important pathological mechanism for the formation of depression. The presence of abnormalities in the white matter integrity of hypothalamic subregions in mild cognitive impairment with depressive symptoms (D-MCI) remains unknown.

Methods: In this study, we used diffusion tensor imaging (DTI) to explore the white matter integrity of hypothalamic subregions in D-MCI.

The increased effective connectivity from left middle occipital gyrus to right medial septum/diagonal bands in AD patients after donepezil intervention.

Introduction: Donepezil enhances the function of cholinergic nerves by increasing the concentration of acetylcholine, thereby improving clinical symptoms in patients with Alzheimer's disease (AD). However, the neural mechanisms of how donepezil modulates the effective connectivity (EC) network of cholinergic system in AD patients remain unknown. We speculated that the effective network of the cholinergic system changes in AD patients after donepezil intervention.

Predicting the efficacy of donepezil intervention in Alzheimer's disease patients using regional homogeneity in the inferior orbitofrontal cortex.

Background: Although donepezil is a commonly used drug for treating Alzheimer's disease (AD), the mechanisms by which it affects patients' functional brain activity, and thus modulates clinical symptoms, remain unclear.

Methods: In the present study, we used resting-state functional magnetic resonance imaging (MRI) and regional homogeneity (ReHo) to investigate the effects of donepezil on local brain activity in AD patients. Resting-state functional MRI data were collected from 32 subjects: 16 healthy controls and 16 AD patients.

Abnormally decreased functional connectivity of the right nucleus basalis of Meynert in Alzheimer's disease patients with depression symptoms.

Dysfunction of the basal forebrain is the main pathological feature in patients with Alzheimer's disease (AD). The aim of this study was to explore whether depressive symptoms cause changes in the functional network of the basal forebrain in AD patients. We collected MRI data from depressed AD patients (n = 24), nondepressed AD patients (n = 14) and healthy controls (n = 20).

3D Printing of Individualized Microfluidic Chips with DLP-Based Printer.

Microfluidic chips have shown their potential for applications in fields such as chemistry and biology, and 3D printing is increasingly utilized as the fabrication method for microfluidic chips. To address key issues such as the long printing time for conventional 3D printing of a single chip and the demand for rapid response in individualized microfluidic chip customization, we have optimized the use of DLP (digital light processing) technology, which offers faster printing speeds due to its surface exposure method. In this study, we specifically focused on developing a fast-manufacturing process for directly printing microfluidic chips, addressing the high cost of traditional microfabrication processes and the lengthy production times associated with other 3D printing methods for microfluidic chips.

The lack of negative association between TE load and subgenome dominance in synthesized allotetraploids.

Polyploidization is important to the evolution of plants. Subgenome dominance is a distinct phenomenon associated with most allopolyploids. A gene on the dominant subgenome tends to express to higher RNA levels in all organs as compared to the expression of its syntenic paralogue (homoeolog).

Research Note: A recombinant duck-derived H6N2 subtype avian influenza virus can replicate and shed in young chickens and cause disease.

The H6N2 subtype avian influenza virus (AIV) is commonly detected in the migratory waterfowl reservoirs. Previously, H6N2 AIV was believed to be nonpathogenic to young chickens and could not infect or shed in their respiratory tract under experimental conditions. However, in present study, a highly recombinant strain of duck-derived H6N2 AIV was discovered and isolated for pathogenicity tests.

Collagen-Hyaluronic Acid Composite Hydrogels with Applications for Chronic Diabetic Wound Repair.

Chronic diabetic wounds have become a major healthcare challenge worldwide. Improper treatment may lead to serious complications. Current treatment methods including biological and physical methods and skin grafting have limitations and disadvantages, such as poor efficacy, inconvenience of use, and high cost.