Exploring the Behavioral Intentions of Hemodialysis Nursing Staff Using Intelligent Information Systems.

This study aims to explore the intention of nursing staff at a dialysis center in a regional hospital in Taiwan to use a smart information system for hemodialysis. A cross-sectional study design was adopted, and data were collected through a questionnaire survey. The questionnaire covered variables such as overall satisfaction with the system, user experience, interface design, functional evaluation, and years of service.

Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas: a rare case report and review of the literature.

Background: Undifferentiated carcinoma with osteoclast-like giant cells of the pancreas (UC-OGCP) is an exceedingly rare malignant tumour that accounts for less than 1% of all pancreatic nonendocrine neoplasms.

Case Presentation: We present a case of UC-OGCP in a 46-year-old male patient who was referred to our hospital after the incidental identification of a cystic tumour in the pancreas. Computed tomography (CT) revealed a 1.

Influence of prolonged exposure to acetamiprid on the toxicology of the decapod crustacean Pacific white shrimp Penaeus vannamei.

Neonicotinoid insecticides have raised increasing concerns due to their toxic effects on aquatic invertebrates, potentially contributing to population declines and aquatic food web disruption. This study investigated the chronic toxicity of the first-generation neonicotinoid acetamiprid on Penaeus vannamei using physiological, biochemical, and transcriptomic approaches. After 28 days of exposure, significant reductions in survival rate, growth performance, and hepatosomatic index were observed, with a calculated 96 h LC of 60.

Mechanical force-induced oncostatin M secretion by Jun-positive neutrophils promotes craniofacial bone regeneration for midface hypoplasia treatment.

Background: Midfacial hypoplasia is a common craniofacial deformity. Trans-sutural distraction osteogenesis (TSDO), which applies mechanical force to stimulate bone formation at the zygomaticomaxillary sutures (ZMS), has emerged as an effective therapeutic strategy. However, the underlying mechanisms of TSDO-induced osteogenesis remain unclear, resulting in prolonged treatment durations and limited clinical application.

Three-dimensional visualization measurement and analysis of human hepatic pedicle based on Laennec's capsule.

Background: Laennec's capsule serves as a crucial anatomical landmark in liver surgery, yet imaging-based morphometric studies of this fibrous membrane remain limited. This study introduces a novel 3D reconstruction technique to visualize and measure the hepatic pedicle via Laennec's capsule, with validation focused on the left portal branch and development of a 3D-printed model for surgical planning.

Methods: 30 patients undergoing left hemihepatectomy were recruited to validate the accuracy of a Laennec's capsule-based 3D reconstruction system (Hisense CAS).

Ecological risks under combined pollution: Toxicological effects of clothianidin and microplastics on Penaeus vannamei.

In real-world conditions, multiple pollutants coexist, making it insufficient to study a single pollutant's effects. Clothianidin, a widely present neonicotinoid insecticide, poses significant ecological risks due to its unique mechanism of action. Similarly, microplastics, another prevalent pollutant, have notable ecological impacts.

Are There High-Density Deep States in an Atomic-Layer-Deposited Indium-Gallium-Zinc Oxide Thin Film?

It has been well recognized that high-density deep states exist in indium-gallium-zinc oxide (IGZO) thin films. Many of the device characteristics of IGZO transistors, such as negative bias illumination stability (NBIS), are understood to be related to these deep states. However, in this work, it was found that the deep-state density () of atomic-layer-deposited (ALD) IGZO transistors can be an ultralow value (<2.

Protocol to Mine Unknown Flanking DNA Using PER-PCR for Genome Walking.

Genome walking, a molecular technique for mining unknown flanking DNAs, has a wide range of uses in life sciences and related areas. Herein, a simple but reliable genome walking protocol named primer extension refractory PCR (PER-PCR) is detailed. This PER-PCR-based protocol uses a set of three walking primers (WPs): primary WP (PWP), secondary WP (SWP), and tertiary WP (TWP).

Combined exposure effects: Multilevel impact analysis of cycloxaprid and microplastics on Penaeus vannamei.

In real environments, multiple pollutants often coexist, so studying the impact of a single pollutant does not fully reflect the actual situation. Cycloxaprid, a new neonicotinoid pesticide, poses significant ecological risks due to its unique mechanism and widespread distribution in aquatic environments. Additionally, the ecological effects of microplastics, another common environmental pollutant, cannot be overlooked.

Additional Lesion Sets in Ablation of Outflow Tract Premature Ventricular Contractions: A Randomized Clinical Trial.

Importance: Recurrence remains a challenge after ablation of outflow tract premature ventricular contractions (OT-PVCs). Although adding additional lesions next to the index effective ablation site is sometimes performed to reinforce the ablation, it remains uncertain whether this approach is effective.

Objective: To test the hypothesis that additional ablation lesions would reduce the recurrence rate compared with single-point ablation at the index effective site for the ablation of OT-PVCs.

GelMA loaded with exosomes from human minor salivary gland organoids enhances wound healing by inducing macrophage polarization.

Non-healing skin wounds pose significant clinical challenges, with biologic products like exosomes showing promise for wound healing. Saliva and saliva-derived exosomes, known to accelerate wound repair, yet their extraction is difficult due to the complex environment of oral cavity. In this study, as a viable alternative, we established human minor salivary gland organoids (hMSG-ORG) to produce exosomes (MsOrg-Exo).

Perioperative and oncologic outcomes of robot-assisted versus open surgery for pancreatic ductal adenocarcinoma: a systematic review and meta-analysis.

This systematic review and meta-analysis aimed to compare perioperative and oncologic outcomes in patients with pancreatic ductal adenocarcinoma (PDAC) treated with robotic-assisted surgery versus open laparotomy. The study followed the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. Randomized controlled trials (RCTs) and cohort studies up to June 15, 2024, were identified using PubMed, EMBASE, and Google Scholar.

Distraction force promotes the osteogenic differentiation of Gli1 cells in facial sutures via primary cilia-mediated Hedgehog signaling pathway.

Background: Trans-sutural distraction osteogenesis (TSDO) involves the application of distraction force to facial sutures to stimulate osteogenesis. Gli1 cells in the cranial sutures play an important role in bone growth. However, whether Gli1 cells in facial sutures differentiate into bone under distraction force is unknown.

Developmental toxicity of the neonicotinoid pesticide clothianidin to the larvae of the crustacean Decapoda, Penaeus vannamei.

The developmental toxicity effects of neonicotinoid pesticides such as clothianidin have not been fully explored in agricultural applications. This is particularly noteworthy because such pesticides significantly impact the survival rates of invertebrates, with arthropod larvae being particularly vulnerable. This study aimed to address this research gap by specifically investigating the toxicological effects of clothianidin on the developmental stages of the larvae of the economically important aquaculture species Penaeus vannamei.

Next-generation neonicotinoid: The impact of cycloxaprid on the crustacean decapod Penaeus vannamei.

Cycloxaprid, a new neonicotinoid pesticide, poses ecological risks, particularly in aquatic environments, due to its unique action and environmental dispersal. This study investigated the ecotoxicological effects of various concentrations of cycloxaprid on Penaeus vannamei over 28 days. High cycloxaprid levels significantly altered shrimp physiology, as shown by changes in the hepatosomatic index and fattening.

Primer extension refractory PCR: an efficient and reliable genome walking method.

Genome walking, a molecular technique for obtaining unknown flanking genomic sequences from a known genomic sequence, has been broadly applied to determine transgenic sites, mine new genetic resources, and fill in chromosomal gaps. This technique has advanced genomics, genetics, and related disciplines. Here, an efficient and reliable genome walking technique, called primer extension refractory PCR (PER-PCR), is presented.

Dinotefuran exposure alters biochemical, metabolomic, gut microbiome, and growth responses in decapoda pacific white shrimp Penaeus vannamei.

Dinotefuran, a neonicotinoid insecticide, may impact nontarget organisms such as Decapoda P. vannamei shrimp with nervous systems similar to insects. Exposing shrimp to low dinotefuran concentrations (6, 60, and 600 μg/L) for 21 days affected growth, hepatosomatic index, and survival.

Protocol to retrieve unknown flanking DNA sequences using semi-site-specific PCR-based genome walking.

Here, we describe a protocol based on semi-site-specific primer PCR (3SP-PCR) to access unknown flanking DNA sequences. We specify the guidelines for designing primers for 3SP-PCR. We also describe experimental procedures for the 3SP-PCR, along with PCR product purification and subsequent sequencing and analysis.

Objective and subjective evaluation of the efficacy of trans-sutural distraction osteogenesis: A correlation analysis between computed tomography measurements and FACE-Q scores.

Previous studies on the efficacy of trans-sutural distraction osteogenesis (TSDO) to treat midface hypoplasia caused by cleft lip and palate (CLP) have mainly focused on objective measurements while ignoring the subjective feelings of patients. This study aimed to analyse the changes in and correlation between computed tomography (CT) measurements and FACE-Q scores in patients who underwent TSDO by performing a comprehensive evaluation from both objective and subjective perspectives. This retrospective study included 25 patients with an average age of 10.

Coexpression analysis of angiogenesis, proliferation, apoptosis, autophagy and SHH pathway genes involved in skin expansion.

Skin and soft tissue expansion is a widely used technique in plastic surgery. However, the regulatory mechanisms associated with cellular processes involved in skin expansion are not well elucidated. In the present study, we aimed at exploring the transcriptome changes associated with skin expansion and profiling the difference in gene expression between the skin tissue in the top of the dilator and the skin tissue in the side of the dilator.

Negative pressure wound therapy for flap closed-incisions after 3D-printed prosthesis implantation in patients with chronic osteomyelitis with soft tissue defects.

Background: The flap closed-incisions healing after 3D-printed prosthesis implantation in Chronic Osteomyelitis with Soft Tissue Defects (COSTD) is critical. This study aimed to explore the safety and effectiveness of Negative Pressure Wound Therapy (NPWT) in promoting flap closed-incisions healing.

Methods: Retrospective analysis of clinical data was performed, including baseline, surgical and hospitalization information.

Regenerative peripheral nerve interface reduces the incidence of neuroma in the lower limbs after amputation: a retrospective study based on ultrasound.

Background: Amputees suffer from symptomatic neuroma and phantom limb pain. Regenerative peripheral nerve interface (RPNI) has recently been regarded as an effective method to prevent neuroma after amputation. However, the verifications of RPNI efficacy are mostly based on subjective evaluation, lacking objective approaches.

Human umbilical cord mesenchymal stem cell-derived exosomes combined with gelatin methacryloyl hydrogel to promote fractional laser injury wound healing.

To investigate whether human umbilical cord mesenchymal stem cell-derived exosomes combined with gelatin methacryloyl (GelMA) hydrogel are beneficial in promoting healing of laser-injured skin wounds in mice. Supernatants of cultured human umbilical cord mesenchymal stem cells (HUC-MSCs) were collected to obtain human umbilical cord MSC-derived exosomes (HUC-MSCs-Exos), which were combined with GelMA hydrogel complex to treat a mouse fractional laser injury model. The study was divided into PBS group, EX (HUC-MSCs-Exos) group, GEL (GelMA hydrogel) group and EX+GEL (HUC-MSCs-Exos combined with GelMA hydrogel) group.