MnOx nanoflower-based ultrasound-responsive drug delivery for sonodynamic therapy of wound infections.

The rapid spread of drug-resistant bacterial infections has become a major global health challenge, particularly in the treatment of deep organ abscesses, which often lead to severe and life-threatening infections. Traditional light-responsive and microenvironment-responsive nanoparticle drug delivery systems (DDSs) have limitations in treating deep abscesses. In contrast, ultrasound (US)-driven sonodynamic therapy (SDT), with its non-invasive, targeted radiation and excellent tissue penetration capabilities, offers great potential for eradicating deep bacterial infections.

Chitosan-based double-network hydrogel with synergistic photothermal/nitric oxide therapy for methicillin-resistance Staphylococcus aureus infected wound healing.

The development of advanced wound dressings that incorporate synergistic antibacterial strategies is essential for addressing antibiotic-resistant infections and facilitating tissue regeneration. This study presents a near-infrared (NIR)-responsive double-network hydrogel (Gel@HS) that combines photothermal therapy (PTT) and nitric oxide (NO) gas therapy for the treatment of methicillin-resistant Staphylococcus aureus (MRSA)-infected wounds. The hydrogel was engineered with acrylamide as the primary network and chitosan, a carbohydrate polymer, as the secondary network, capitalizing on chitosan's natural tissue adhesion and antibacterial properties.

Thermoresponsive Biodegradable Hydrogel Combined with Photothermal and Chemodynamic Therapies To Eliminate Biofilms and Accelerate Infected Wound Healing.

Bacterial wound infection tends to cause intense inflammation, while a lack of oxygen can lead to the destruction and contraction of blood vessels in the wound microenvironment. Here, a degradable hydrogel (denoted as PBCB hydrogel) was constructed with gelatin methacrylate (GelMA), tannic acid (TA), and polyphosphate (PolyP) by photopolymerization, and mesoporous polydopamine nanoparticles (MPDA) with berberine (BR), along with Cu and Bi nanoparticles grown in situ, were loaded into the hydrogel. PBCB hydrogel exhibits a high photothermal conversion efficiency (68.

ORL@Cu-MOF Boost Cuproptosis and Suppress Fatty Acid Metabolism for Cancer Lymph Node Metastasis Synergistic Therapy.

Lymph node metastasis (LNM) is one of the significant characteristics of poor prognosis in oral squamous cell carcinoma (OSCC), strongly associated with high mortality rates. Immunotherapy has emerged as a crucial treatment modality for OSCC LNM, yet its limited response rate severely restricts its clinical application. Cuproptosis, a newly discovered immunogenic cell death (ICD), is often hindered by lipid metabolic reprogramming in tumor cells, which also contributes significantly to the lack of response to immunotherapy.

Transcriptome Sequencing Reveals Survival Strategies and Pathogenic Potential of Under Gastric Acid Stress.

As a common food-borne pathogen, comes into direct or indirect contact with gastric acid after ingestion. However, the mechanisms by which passes through the gastric acid barrier, recovers, and causes pathogenicity remain unclear. In this study, static in vitro digestion simulation experiments showed that some strains can pass through the gastric acid barrier by utilizing microacid tolerance mechanisms and altering their survival state.

Metal-Phenolic Network Hydrogel Vaccine Platform for Enhanced Humoral Immunity against Lethal Rabies Virus.

Rabies, caused by rabies virus (RABV), is a zoonotic disease with a high mortality rate that has attracted global attention with the goal of eradication by 2030. However, rabies can only be prevented by appropriate and multiple vaccinations, which impede widespread vaccination in developing countries due to its high expenditure. Designing single-dose vaccines is a pressing challenge in the prevention of rabies and other infectious diseases.

Photothermal-Driven α-Amylase-Modified Polydopamine Pot-Like Nanomotors for Enhancing Penetration and Elimination of Drug-Resistant Biofilms.

Biological enzyme-functionalized antibacterial nanoparticles, which can degrade biofilm and kill bacteria under mild reaction conditions, have attracted much attention for the elimination of deep-seated bacterial infections. However, the poor diffusion and penetration capabilities of recently developed biological enzyme-functionalized antibacterial nanoparticles in biofilm severely impair the eradication efficacy of deep-seated bacteria. Herein, a photothermal-driven nanomotor (denoted as APPNM) is developed for enhancing the elimination of drug-resistant biofilms and the eradication of deep-seated bacteria.

Tetramethylpyrazine attenuates sodium arsenite-induced acute kidney injury by improving the autophagic flux blockade via a YAP1-Nrf2-p62-dependent mechanism.

With increased application, sodium arsenite (AS III)-induced acute kidney injury (AI-AKI) is becoming a new clinical challenge, but its potential pathogenesis remains poorly studied. Our previous data demonstrated that inducing autophagy and mitochondrial dysfunction in renal tubular cells are important links of AI-AKI and could be inhibited by tetramethylpyrazine (TMP). Recently, co-transcription factor YAP1 is reported to control autophagy and is mandatory to stimulate autophagic flux.

Dahuang chuanxiong decoction against contrast-induced nephropathy: Multi-omics, crosstalk between BNIP3-mediated mitophagy and IL-17 pathway.

Background: Contrast-induced nephropathy (CIN), also known as contrast-induced acute kidney injury (CI-AKI), represents a prevalent form of hospital-acquired renal injury. However, the mechanisms underlying its pathogenesis remain unclear. Based on our previous research findings, the Dahuang Chuanxiong decoction (DCH), composed of Radix et Rhizoma Rhei (DH) and Rhizoma Chuanxiong (CX), has demonstrated efficacy for inhibiting CI-AKI by attenuating oxidative stress and apoptosis in renal tubular epithelial cells.

Effects of low-salt stress on biological characteristics and transcriptomic profiles of Vibrio parahaemolyticus.

Studies have proved that halophilic Vibrio parahaemolyticus is widely detected in freshwater environments (salinity <0.5 %). However, the growth and colonization of V.

Antimicrobial materials based on photothermal action and their application in wound treatment.

Considering the increasing abundance of antibiotic-resistant bacteria, novel antimicrobial approaches need to be investigated. Photothermal therapy (PTT), an innovative noninvasive therapeutic technique, has demonstrated significant potential in addressing drug-resistant bacteria and bacterial biofilms. However, when used in isolation, PTT requires higher-temperature conditions to effectively eradicate bacteria, thereby potentially harming healthy tissues and inducing new inflammation.

Cobalt-Catalyzed Asymmetric Migratory Nozaki-Hiyama-Kishi Coupling.

Selective functionalization of ubiquitous C-H bonds based on 1,-metal migration provides an attractive and sustainable route to access complex molecules from readily available precursors. Herein, we report a Co-catalyzed asymmetric reductive migratory Nozaki-Hiyama-Kishi (NHK) coupling between two readily available electrophiles, aryl (pseudo)halides and aldehydes, via an unprecedented through-space aryl-to-alkenyl 1,4-cobalt/hydride shift. The judicious choice of ligands is crucial for selectivity, leading to either ipso- or migratory NHK products with exquisite control of regio-, /-, and enantioselectivity.

A two-in-one molybdenum disulfide-chitosan nanoparticles system for activating plant defense mechanisms and reactive oxygen species to treat Citrus Huanglongbing.

Citrus Huanglongbing (HLB) poses an enormous challenge to Citrus cultivation worldwide, necessitating groundbreaking interventions beyond conventional pharmaceutical methods. In this study, we propose molybdenum disulfide-chitosan nanoparticles (MoS-CS NPs) through electrostatic adsorption, preserving the plant-beneficial properties of molybdenum disulfide (MoS), while enhancing its antibacterial effectiveness through chitosan modification. MoS-CS NPs exhibited significant antibacterial efficacy against RM1021, and the closest relatives to Candidatus Liberibacter asiaticus (CLas), Erwinia carotovora, and Xanthomonas citri achieved survival rates of 7.

Fabrication of nanogels to improve the toxicity and persistence of cycloxaprid against Diaphorina citri, the vector of citrus huanglongbing.

Introduction: Diaphorina citri is the most serious pest of citrus worldwide because it is the natural insect vector of huanglongbing. Cycloxaprid (Cyc) was highly toxic to D. citri.

Efficacy and Safety of Insulin Degludec/Insulin Aspart versus Biphasic Insulin Aspart 30 in Patients with Type 2 Diabetes: A Meta-Analysis of Randomized Controlled Trials.

Background: We systematically reviewed and analyzed the efficacy and safety of insulin degludec/insulin as-part (IDegAsp) versus biphasic insulin aspart 30 (BIAsp 30) in patients with type 2 diabetes (T2D).

Methods: We used computers to search the Embase, PubMed, Clinical Trials, and the Cochrane Library database, and collected randomized controlled trials (RCTs) on the treatment of IDegAsp versus BIAsp 30 in T2D patients. The research period was from the establishment of the database to May 19, 2023.

Self-assembled carbon monoxide nanogenerators managing sepsis through scavenging multiple inflammatory mediators.

Sepsis, a life-threatening syndrome of organ damage resulting from dysregulated inflammatory response, is distinguished by overexpression of inflammatory cytokines, excessive generation of reactive oxygen/nitrogen species (RONS), heightened activation of pyroptosis, and suppression of autophagy. However, current clinical symptomatic supportive treatment has failed to reduce the high mortality. Herein, we developed self-assembled multifunctional carbon monoxide nanogenerators (Nano CO), as sepsis drug candidates, which can release CO in response to ROS, resulting in clearing bacteria and activating the heme oxygenase-1/CO system.

Uropathogenic infection: innate immune disorder, bladder damage, and Tailin Fang II.

Background: Uropathogenic (UPEC) activates innate immune response upon invading the urinary tract, whereas UPEC can also enter bladder epithelial cells (BECs) through interactions with fusiform vesicles on cell surfaces and subsequently escape from the vesicles into the cytoplasm to establish intracellular bacterial communities, finally evading the host immune system and leading to recurrent urinary tract infection (RUTI). Tailin Fang II (TLF-II) is a Chinese herbal formulation composed of botanicals that has been clinically proven to be effective in treating urinary tract infection (UTI). However, the underlying therapeutic mechanisms remain poorly understood.

HO self-supplying and GSH-depleting nanosystem for amplified NIR mediated-chemodynamic therapy of MRSA biofilm-associated infections.

Reactive oxygen species (ROS) has emerged as potent therapeutic agents for biofilm-associated bacterial infections. Chemodynamic therapy (CDT), involving the generation of high-energy ROS, displays great potential in the therapy of bacterial infections. However, challenges such as insufficient hydrogen peroxide (HO) and over-expressed glutathione (GSH) levels within the microenvironment of bacterial biofilms severely limit the antibacterial efficacy of CDT.

Tetramethylpyrazine attenuates renal tubular epithelial cell ferroptosis in contrast-induced nephropathy by inhibiting transferrin receptor and intracellular reactive oxygen species.

Contrast-induced nephropathy (CIN) is a leading cause of hospital-acquired acute kidney injury (AKI). Recently, ferroptosis was reported to be crucial for AKI pathogenesis. Our previous studies indicated antioxidant tetramethylpyrazine (TMP) prevent CIN in vivo.

Pore- and Core-Scale Recovery Performance of Consortium Bacteria from Low-Permeability Reservoir.

Performance evaluation of microorganisms that have emulsifying and degrading effects on crude oil has been extensively conducted in the laboratory. However, the ultimate goal of microbial enhanced oil recovery is field application, so the pilot simulation experiments are crucial. In this study, a micro-visualization model and the real cores were chosen to investigate the actual recovery efficiency and the mechanism of the consortium bacteria B-ALL, which has been proven to have good emulsification and degradation effects in lab studies in porous media.

EEGNet-based multi-source domain filter for BCI transfer learning.

Deep learning has great potential on decoding EEG in brain-computer interface. While common deep learning algorithms cannot directly train models with data from multiple individuals because of the inter-individual differences in EEG. Collecting enough data for each subject to satisfy the training of deep learning would result in an increase in training cost.