A Set of Fluorescent Protein-Based Markers for Major Vesicle Coat Proteins in Yeast.

In eukaryotic cells, vesicle-mediated transport interconnects the endomembrane system. These vesicles are formed by coat proteins via deformation of donor membranes. Here, we constructed a set of fluorescent protein-based markers for major coat protein complexes in the yeast model system, and examined their subcellular localization patterns.

Rational spatial rewiring of key enzymes enhances α-santalene production in Saccharomyces cerevisiae.

Spatial compartmentalization in eukaryotic cell factories often constrains the efficiency of metabolic pathways. Here, we systematically mapped the subcellular localization of nine core enzymes in the α-santalene biosynthetic pathway of Saccharomyces cerevisiae, identifying metabolic bottlenecks associated with nuclear and endoplasmic reticulum (ER) localization. Through rational spatial engineering, including bioinformatically guided HMG1 truncation to achieve ER release and nuclear export signal (NES) tagging of key enzymes, we successfully rewired enzyme localization to enhance pathway flux.

Polyphenol Conductive Nanozyme-Enhanced Redox Hydrogel Coupled with Vagus Nerve Electrical Stimulation for Diabetic Wound Repair.

Diabetic wound repair is complicated by excessive oxidative reactions, bacterial infection, and persistent inflammation. Nanozyme-incorporated hydrogels have shown potential to facilitate diabetic wound repair. This study developed a polyphenol-conductive nanozyme-enhanced redox hydrogel composed of dopamine-grafted fish gelatin, methacrylated silk fibroin, and dopamine-mediated poly(3,4-ethylenedioxythiophene) (PDA-Fe-PEDOT) conductive nanozymes.

Recruitment of Atg1 to the phagophore by Atg8 orchestrates autophagy machineries.

Autophagy-related (Atg) proteins catalyze autophagosome formation at the phagophore assembly site (PAS). The assembly of Atg proteins at the PAS follows a semihierarchical order, in which Atg8 is thought to be quite downstream but still able to control the size of autophagosomes. Yet, how Atg8 coordinates multiple branches of autophagy machinery to regulate autophagosomal size is not clear.

Asgard Arf GTPases can act as membrane-associating molecular switches with the potential to function in organelle biogenesis.

Inward membrane budding, i.e., the bending of membrane towards the cytosol, is essential for forming and maintaining eukaryotic organelles.

Polyphenol Microneedles for Dermatological Therapy.

Dermatological diseases represent one of the most prevalent health concerns globally, with associated care and treatment costs having consistently increased over the past decade. Current therapeutic modalities, including topical medications, oral pharmaceuticals, and physical therapies, frequently face challenges such as inadequate drug absorption and substantial adverse effects. Microneedles, recognized as minimally invasive drug delivery systems, effectively penetrate the cutaneous barrier to enhance drug permeability and therapeutic efficacy.

Study of the mechanical compression properties of Rosa sterilis S.D. Shi based on FEM.

Understanding the mechanical properties of Rosa sterilis S.D. Shi is important for the design and improvement of related mechanical equipment for planting, picking, processing, and transporting Rosa sterilis S.

Inhibition of mitochondrial energy production leads to reorganization of the plant endomembrane system.

Mitochondria have generated the bulk of ATP to fuel cellular activities, including membrane trafficking, since the beginning of eukaryogenesis. How inhibition of mitochondrial energy production will affect the form and function of the endomembrane system and whether such changes are specific in today's cells remain unclear. Here, we treated Arabidopsis thaliana with antimycin A (AA), a potent inhibitor of the mitochondrial electron transport chain (mETC), as well as other mETC inhibitors and an uncoupler.

Blocking the p38 MAPK Signaling Pathway in the Rat Hippocampus Alleviates the Depressive-like Behavior Induced by Spinal Cord Injury.

Patients with spinal cord injury (SCI) may develop depression, which can affect their rehabilitation. However, the underlying mechanism of depression in SCI patients remains unclear. Previous studies have revealed increased p38 MAPK phosphorylation in the rat hippocampus after SCI, accompanied by depression-like behaviors.

An improved reverse genetics system for rotavirus vaccine strain LLR using five plasmid vectors.

Species A rotaviruses (RVs), which belong to the family and contain a genome of 11 segmented dsRNA segments, are a leading cause of severe acute gastroenteritis in infants and children younger than 5 years of age. We previously developed a strategy to recover rotavirus vaccine strain LLR from 11 cloned plasmids. Here, we report an improved reverse genetics system for LLR by combining two or three transcriptional cassettes in a single plasmid, which substantially enhances rescue efficiency from 66.

Temporal dissection of the roles of Atg4 and ESCRT in autophagosome formation in yeast.

Autophagosomes are formed by the enlargement and sealing of phagophores. This is accompanied by the recruitment and release of autophagy-related (Atg) proteins that function therein. Presently, the relationship among factors that act after the initial emergence of the phagophore is unclear.

Willingness of medical students to work on the COVID-19 frontline during the pandemic in China: A nationwide population-based cross-sectional study.

Background: The World Health Organization declared that coronavirus disease 2019 (COVID-19) constitutes an international public health emergency, which has strained health resources. In this study, we aimed to understand medical students' willingness to join the workforce fighting against the COVID-19 pandemic and identify factors associated with their decisions.

Methods: We conducted a nationwide cross-sectional study using the Wen-Juan-Xing platform and a pre-designed questionnaire from 23 March to 19 April 2021.

Arachnoid Cyst Associated With Traumatic Chronic Subdural Hemorrhage in Children.

Introduction: It was reported that most arachnoid cyst (AC)-associated chronic subdural hematoma (CSDH) was spontaneous. However, AC with CSDH caused by trauma was extremely scarcely reported. In this paper, the authors reported a successful surgical treatment of a case of AC associated with CSDH after trauma.

Establishment of a Reverse Genetics System for Rotavirus Vaccine Strain LLR and Developing Vaccine Candidates Carrying VP7 Gene Cloned From Human Strains Circulating in China.

Human rotavirus A (RVA) causes acute gastroenteritis in infants and young children. The LLR RVA vaccine, which licensed in 2000 and widely used in China, significantly reduced rotavirus disease burden in China. With the changing of RV circulating strains and the emergence of new genotypes, the LLR vaccine against RVGE needed to be upgraded.

Template plasmids optimized for deletion of multiple genes in yeast .

Unlabelled: For , gene knockout is routinely performed by transformation with a linear DNA cassette consisting of a selection marker gene flanked by upstream and downstream sequences homologous to a target gene. Over the years, several plasmid sets containing a variety of selection marker genes have been developed. Targeting fidelity under this strategy was high when performing the first gene knockout in a strain.

Oxygen-Propelled Dual-Modular Microneedles with Dopamine-Enhanced RNA Delivery for Regulating Each Stage of Diabetic Wounds.

Diabetic wounds are characterized by the disruption and cessation of essential healing stages, which include hemostasis, inflammation, proliferation, and remodeling. However, traditional treatments for diabetic wounds concentrate on individual stages of the healing process. Herein, this study utilizes mask-mediated sequential polymerization and varied cross-linking techniques to develop dual-modular microneedles (MNs) with fast- and slow-module, exhibiting varying degradation rates tailored for the full spectrum of diabetic wound healing.

Assessing the Presence of Phosphoinositides on Autophagosomal Membrane in Yeast by Live Cell Imaging.

The formation of autophagosomes mediating the sequestration of cytoplasmic materials is the central step of autophagy. Several phosphoinositides, which are signaling molecules on the membrane, are involved in autophagy. However, it is not always clear whether these phosphoinositides act directly at the site of autophagosome formation, or indirectly via the regulation of other steps or pathways.

Image processing based modeling for Rosa roxburghii fruits mass and volume estimation.

The mass and volume of Rosa roxburghii fruits are essential for fruit grading and consumer selection. Physical characteristics such as dimension, projected area, mass, and volume are interrelated. Image-based mass and volume estimation facilitates the automation of fruit grading, which can replace time-consuming and laborious manual grading.

Dietary calcium intake at breakfast is associated with a lower risk of cognitive impairment than at dinner in Chinese adults: the CHNS cohort study.

Background And Objectives: If the proportion of calcium intake over a whole day is related to the risk of cognitive impairment in adults is still largely unknown. This research aimed to examine the relation of dietary calcium intake at dinner versus breakfast with the risk of cognitive impairment by using data from the China Health and Nutrition Survey (CHNS).

Methods And Study Design: A total of 2,099 participants (including 668 cognitive impairment) in the CHNS (1997-2006) were included.

Elevated HSPB1 Expression Is Associated with a Poor Prognosis in Glioblastoma Multiforme Patients.

Background:  Glioblastoma multiforme (GBM) is a highly aggressive form of brain cancer. This study investigated the clinical predictive value of heat shock protein β1 (HSPB1) in patients with GBM.

Methods:  A correlation was established between HSPB1 expression and GBM progression using data from The Cancer Genome Atlas (TCGA) dataset, Chinese Glioma Genome Atlas dataset, Gene Expression Omnibus dataset, and Human Protein Atlas database.

Spatiotemporal Epidemiological Trends of Mpox in Mainland China: Spatiotemporal Ecological Comparison Study.

Background: The World Health Organization declared mpox an international public health emergency. Since January 1, 2022, China has been ranked among the top 10 countries most affected by the mpox outbreak globally. However, there is a lack of spatial epidemiological studies on mpox, which are crucial for accurately mapping the spatial distribution and clustering of the disease.