Impact of remote follow-up under an intelligent medical collaboration model on health promotion and clinical outcomes in patients with urinary calculi.

Objectives: Urinary calculi are characterized by a high recurrence rate, and patients' adherence to self-management after discharge directly affects health outcomes. Traditional offline follow-up models often face problems such as poor compliance and uneven allocation of medical resources, making it difficult to meet individualized health management needs. Remote follow-up provides a novel solution to optimize long-term management, improve health literacy, and enhance clinical outcomes.

The public's perceptions and attitudes of male nursing postgraduate professional identity on Chinese social media: Qualitative study based on machine learning.

Background: Professional identity plays a critical role in the career development of male postgraduate nursing students, particularly in contexts where gender imbalance and social stereotypes persist.

Objective: This study explores how the clinical professional identity of male nursing postgraduates is perceived and constructed through social media discourse in China.

Design: A qualitative study using content analysis of social media discourse, supported by sentiment classification and clustering algorithms.

The Impact of Periodontitis on Cardiovascular Disease: Mechanisms, Evidence, and Therapeutic Implications.

Periodontitis, a highly prevalent chronic inflammatory disease caused by bacteria. Cardiovascular disease (CVD) is responsible for more than 17 million deaths globally each year. Both periodontitis and CVD are global noncommunicable diseases that share common risk factors.

A study on the mechanism of Bushen Kaiqiao Formula in modulating microglial activation to alleviate neuroinflammation in ADHD.

Background: Growing evidence suggests that dysregulated microglial activation and neuroinflammation in the prefrontal cortex may underlie the pathophysiology of Attention Deficit Hyperactivity Disorder. Utilizing spontaneously hypertensive rats-a well-validated animal model of ADHD-this study aimed to characterize ADHD-like behavioral phenotypes and prefrontal cortical neuronal development and elucidate the neuroimmunological mechanisms through which the Bushen Kaiqiao Formula (BSKQF) exerts its therapeutic effects by modulating microglial activation states.

Methods: Ultra-high-performance liquid chromatography coupled with a tandem mass spectrometer (UHPLC-MS/MS) was used to qualitatively analyze the chemical composition of Bushen Kaiqiao Formula, and network pharmacology was applied to predict its potential therapeutic targets and ADHD-related pathways ADHD-like behaviors, including hyperactivity, impulsivity, spatial memory, and attention, were assessed using the Open Field Test, Elevated Plus Maze, and Y-Maze Test at weeks 2 and 4.

Research progress of dissolving microneedles in the field of component administration of traditional Chinese medicine.

In recent years, dissolving MNS technology, as an emerging transdermal drug delivery technology, has shown unique advantages and broad application prospects in the fields of transdermal drug delivery, transcutaneous immunity, beauty and skin care, food testing, and disease diagnosis. The active ingredients of traditional Chinese medicine have shown remarkable efficacy in treating various diseases. However, the mode of administration of traditional Chinese medicine (TCM) limits its potential for clinical application and promotion to a certain extent.

Engineered Biomimetic Nanovesicles Derived From Bone Marrow Stromal Cells With Innate Homing Capability for Targeted Delivery.

Precise delivery of pharmaceuticals administered to bone marrow for various bone diseases is challenging, given the bone marrow-blood barrier (MBB). Bone marrow stromal cells (BMSCs) derived from bone marrow can naturally infiltrate the MBB and home to bone tissue. Here, biomimetic nanovesicles (namely mNVs) engineered with the extracted cell membrane from BMSCs are reported for homing delivery of different core nanomedicines to bone marrow.

Effects of different exercise modes on the risk factors of arteriosclerosis in postmenopausal women: A systematic review and network meta-analysis.

Arteriosclerosis is one of the most common diseases that progresses to cardiovascular disease in ageing postmenopausal women. Early changes away from the poor lifestyle choices and the active management of risk factors can improve the survival of postmenopausal women. A network meta-analysis was performed to compare the effects of different exercise modes on the risk factors for arteriosclerosis in postmenopausal women.

PDSI_CMIP6: an ensemble CMIP6-projected self-calibrated Palmer drought severity index dataset.

Drought, characterized by below-average water supply, profoundly affects regional water resources and various ecosystem services. The Palmer Drought Severity Index (PDSI) is a widely used metric for drought monitoring and climate change assessments but suffers from inherent climatic inconsistencies and lacks comprehensive and reliable estimates under changing climate conditions. Here we develop a monthly multi-model and multi-scenario dataset of self-calibrated PDSI for the period 1850-2094, derived from 11 climate model outputs within the Coupled Model Intercomparison Project 6 (PDSI_CMIP6).

Effect of different size suspended particles on the bioaccumulation of polycyclic aromatic hydrocarbons in zebrafish tissues.

Polycyclic aromatic hydrocarbons (PAHs) are easily bound with suspended particles (SPS) in rivers, and this will further affect their bioaccumulation. However, it is unclear whether SPS will affect the PAH bioaccumulation in different fish tissues. In the present work, we studied the effect of different size SPS on the PAH bioaccumulation in different zebrafish tissues.

AAV6 vectors provide superior gene transfer compared to AAV9 vectors following intramyocardial administration.

Cardiac gene therapy using adeno-associated viral (AAV) vectors holds great promise for treating heart diseases but would benefit from more potent AAV vectors. Vectors based on the AAV serotypes 6 and 9 have been used in pre-clinical gene therapy studies, yet the therapeutic outcomes varied depending on the experimental model and delivery route used. Here, we evaluated the transduction efficiency of AAV6, AAV9, and AAV9-derived MyoAAVs for local cardiac delivery.

Multi-omics data reveal that SAA1 + fibroblasts exacerbate periodontitis by regulating macrophage inflammation and chemotaxis.

Background: Traditional techniques are limited in their ability to analyze the complex interaction mechanisms among multiple cell types within the periodontal microenvironment, thereby restricting the development of targeted therapies for periodontitis (PD). Utilizing multiomics technologies to investigate the interaction networks of key cell clusters can systematically uncover regulatory mechanisms and identify critical therapeutic targets.

Methods: Through integrative analysis of single-cell RNA sequencing (scRNA-seq), spatial transcriptomics, and bulk transcriptome datasets from periodontal tissues, we systematically characterized the spatial architecture and intercellular communication networks within the inflammatory periodontal microenvironment, identifying a functionally serum amyloid A1 + fibroblasts (SAA1 + Fib) that critically drives disease progression.

Entanglement of spin transition and structural adaptability: manipulating the slow spin equilibrium by the guest-mediated fine-tuning of elastic frustration.

A comprehensive analysis of physical and chemical properties using the same family of spin-crossover complexes is crucial for understanding and designing structure-property relationships. However, finding an appropriate system remains challenging. Here, a series of guest-saturated states based on the 2D Hofmann-type framework [Fe(prentrz)Pd(CN)]·guest (prentrz = (1,2)-3-phenyl--(4-1,2,4-triazol-4-yl)prop-2-en-1-imine; 1·guest) is reported, exhibiting a guest-manipulated slow dynamic effect on spin equilibrium in an incomplete two-step spin-crossover (SCO) process.

Optimized cesarean techniques, IVF use, and foster strain selection enhance germ-free mouse production efficiency.

This study aims to refine sterile cesarean section techniques, in vitro fertilization (IVF) and foster mother selection strategies to enhance the efficiency of germ-free (GF) mouse production. We conducted the following analyses: (1) comparing different cesarean section methods, (2) evaluating the impact of natural mating versus in vitro fertilization (IVF) for obtaining donor mice, and (3) assessing maternal care among three inbred strains (C57BL/6J, BALB/c, NSG) and one outbred strain (KM) as GF foster mothers. The results showed that optimizing surgical methods which preserve the female reproductive tract during cesarean section (FRT-CS), significantly improved fetal survival rates while maintaining sterility.

Construction of the Lactone-Bridged Scaffold of Alstoscholactine through an Acid-Catalyzed Prins Cyclization/Lactonization Cascade.

Described herein is an acid-catalyzed Prins cyclization/lactonization cascade reaction of malonate-tethered inol-3-ylmethanol-olefin substrates, which rapidly constructs the lactone-bridged scaffold of the monoterpenene indole alkaloid alstoscholactine. This reaction demonstrates a wide substrate scope and functional group tolerance, and a catalytic asymmetric reaction provides high enantioselectivity. This work provides a powerful method to access the key framework of alstoscholactine and supports the proposed plausible biogenetic pathway.

Intercalation Chemistry Awakens Transition Metal Hydroxide for Boosted and Sustained Electrocatalytic Sulfion Oxidation.

Electrocatalytic sulfion oxidation reaction (SOR) is a sustainable approach to treat sulfion-rich sewage for S recovery. Transition metal hydroxide (TMOH), utilized in various electrocatalysis, yet is clumsy in SOR due to overstrong adsorption toward S-containing intermediates from unsuitable electronic structure. Herein we report, for the first time, that the SOR performance of TMOH can be awakened by intercalation chemistry.

The hepatic expression status of hepatocyte nuclear factor 4α in subclinical and clinical ketotic dairy cows and its impact on lipid metabolism in hepatocytes.

Due to sustained lipolysis, excessive nonesterified fatty acid (NEFA) concentrations are a common pathological factor of ketosis in peripartal cows. The NEFA provide an energy source to the liver during the transition period, but when in excess they can induce hepatic steatosis and liver dysfunction. In nonruminants, hepatocyte nuclear factor 4α (HNF4α) is involved in lipid metabolism processes in the liver.

Transcriptomic analysis reveals differential gene expression in spleen cells of Chinese soft-shelled turtle infected with Bacillus cereus BC12.

Bacillus cereus infection has emerged as a leading cause of high mortality of Chinese soft-shelled turtle (Pelodiscus sinensis) in aquaculture. Elucidating the complex physiological processes and differentially expressed genes (DEGs) of P. sinensis in response to bacterial pathogens will help us explore strategies to combat bacterial infection.

Contrasting Roles of Intraspecific Trait Variability and Species Turnover in Shaping Functional Composition During Vegetation Restoration on the Loess Plateau.

Understanding how ecological communities respond to environmental variation is one of the fundamental goals in ecology. Both intraspecific trait variability (ITV) and species turnover contribute to shaping community functional composition, yet their relative importance in mediating community responses remains insufficiently resolved. We investigated the roles of ITV and species turnover in influencing community functional composition under natural and plantation restoration on the Loess Plateau.

The Effects of UV-LED Technology on the Quality of Ready-to-Eat Pomegranates: Epigenetic Indicators and Metabolomic Analysis.

Pomegranates are rich in nutrients and classified among ready-to-eat fruits and vegetables. Although this ready-to-eat produce offers convenience, it presents risks associated with pathogenic microorganisms, highlighting the need for pre-sale disinfection. Ultraviolet light-emitting diodes (UV-LEDs) constitute an innovative non-thermal processing technology for food products, offering reduced heat generation and lower energy consumption compared to traditional ultraviolet (UV) irradiation methods.

Cumulative genetic effects of HPA axis on social phobia scrutiny fear: psychoticism and social face as mediators.

Background: Social phobia scrutiny fear is a stress response triggered by perceptions of social evaluation. However, the role of genetic polymorphisms in the Hypothalamic Pituitary Adrenal (HPA) axis in shaping this fear is not well understood. This study investigates how the cumulative genetic score of the HPA axis influences social phobia scrutiny fear.

Tumor-educated platelets: A new field in breast cancer diagnosis.

Tumor-educated platelets (TEPs) represent a novel approach in liquid biopsy, offering new perspectives for diagnosing, treating, and monitoring breast cancer. Unlike traditional histopathological biopsies-the current diagnostic gold standard-TEP-based detection is minimally invasive, requiring only peripheral blood samples and overcoming limitations of repeated invasive procedures. TEPs reflect a dynamic interplay between tumor cells, platelets, and the tumor microenvironment: tumors alter platelet expression profiles while TEPs provide comprehensive molecular information about tumor genetics.

Polyethylene microplastic: impacts on ryegrass seed germination and seedling development.

Microplastic pollution has emerged as a critical global environmental concern, particularly within agricultural ecosystems where its impact on forage production is highly significant. This study used a hydroponic system to investigate the potential effect of polyethylene (PE) microplastics at different concentrations (20, 50, 100, 200, 500, and 1000 mg/L) and particle sizes (200 nm, 25 μm, and 200 μm) on the growth of perennial ryegrass (Lolium perenne L.), with no added PE microplastics (0 mg/L) as a control.

Unsupervised SAM segmentation of zebrafish body: Application to melanin analysis.

Zebrafish have always been a valuable model for studies on human health. Their transparency makes it highly suitable for observing melanin synthesis. Moreover, their high genetic similarity to humans facilitates the study of human diseases, including pigmentation disorders.

Asymmetric shifts in precipitation due to urbanization across global cities.

Urbanization alters precipitation patterns by modifying thermal, dynamic, and chemical processes in the atmosphere. However, its effect on precipitation regimes, particularly at the sub-daily scale, is poorly understood. In this work, we use a high-resolution, spatially continuous satellite precipitation dataset to examine urbanization-induced shifts across precipitation intensities over global cities.