Cancer Therapy with Nanoparticle-Medicated Intracellular Expression of Peptide CRM1-Inhibitor.

Introduction: Peptides can be rationally designed as non-covalent inhibitors for molecularly targeted therapy. However, it remains challenging to efficiently deliver the peptides into the targeted cells, which often severely affects their therapeutic efficiency.

Methods: Herein, we created a novel non-covalent peptide inhibitor against nuclear export factor CRM1 by a structure-guided drug design method and targetedly delivered the peptide into cancer cells by a nanoparticle-mediated gene expression system for use as a cancer therapy.

Efficient degradation of polyacrylate containing wastewater by combined anaerobic-aerobic fluidized bed bioreactors.

Polyacrylate containing wastewater (PCW) is the typical sewage discharged by the textile industry. It has extremely poor biodegradability, and chemical methods were used conventionally as the only way for treating PCW. This study is demonstrating a novel biological method.

Inhibitory Effect of Immunologically Activated Mesenchymal Stem Cells on Lung Cancer Cell Growth and Metastasis.

Mesenchymal stem cells (MSCs) could inhibit the proliferation of lung cancer cells. The authors' study investigated the effects of immunologically activated human umbilical cord (HUC)-MSCs on A549 lung cancer cells. HUC-MSCs were separated from the umbilical cord using the adherence method.

Complete plastid genome sequence of (Schltr.) Szlach. (Orchidaceae), an endemic herb in China.

The species , belonging to the family Orchidaceae, is a small lithophytic herb endemic in south China with significant conservation values. The complete plastid genome sequence of reported here is 144,989 bp in length, with a large single copy (LSC) region of 83,920 bp, a small single copy (SSC) region of 13,063 bp, and a pair of inverted repeat (IRa and IRb) regions of 24,003 bp each. The plastome consists of 95 genes, including 72 protein-coding genes, 4 ribosomal RNA genes, and 19 transfer RNA genes.

Semisynthesis and Biological Evaluation of Platencin Thioether Derivatives: Dual FabF and FabH Inhibitors against MRSA.

The discovery and clinical use of multitarget monotherapeutic antibiotics is regarded as a promising approach to reduce the development of antibiotic resistance. Platencin (PTN), a potent natural antibiotic initially isolated from a soil actinomycete, targets both FabH and FabF, the initiation and elongation condensing enzymes for bacterial fatty acid biosynthesis. However, its further clinical development has been hampered by poor pharmacokinetics.

Baicalin attenuates XRCC1-mediated DNA repair to enhance the sensitivity of lung cancer cells to cisplatin.

Baicalin plays important roles in different types of cancer. A previous report showed that baicalin attenuates cisplatin resistance in lung cancer. However, its mechanism remains unclear.

Chiral Analysis of Lactate during Direct Contact Coculture by Single-Cell On-Probe Enzymatic Dehydrogenation Derivatization: Unraveling Metabolic Changes Caused by d-Lactate.

noncontact cell-based coculture models are frequently employed to study cell-to-cell communication. However, these models cannot accurately represent the complexity of signaling. d-Lactate is an unusual metabolite produced and released by cancer cells.

The effects of acarbose therapy on reductions of myocardial infarction and all-cause death in T2DM during 10-year multifactorial interventions (The Beijing Community Diabetes Study 24).

To investigate the potential benefits of acarbose therapy on cardiovascular events (CVD) in Type 2 diabetes (T2DM) in an urban community over 10-year follow-up. The study population of Beijing Community Diabetes Study (BCDS) were type 2 diabetes (T2DM) living in 21 communities in Beijing. All patients received comprehensive intervention in accordance with the Chinese guidelines for the prevention and treatment of diabetes.

Ferroptosis-Inhibitory Effect and Possible Mechanisms of Ellagitannin Geraniin.

The search for safe and effective ferroptosis-inhibitors has become an important topic. Geraniin, an ellagitannin bearing hexahydroxydiphenoyl (HHDP) and dehydrohexahydroxydiphenoyl (DHHDP) groups, was observed to inhibit erastin-induced ferroptosis in bone marrow-derived mesenchymal stem cells (bmMSCs). To determine the mechanism, geraniin was further analyzed using UV-vis spectra and several colorimetric assays, where its IC values were always much lower than that of the Trolox positive control.

Circular RNA FLNA acts as a sponge of miR-486-3p in promoting lung cancer progression via regulating XRCC1 and CYP1A1.

Significantly high-expressed circFLNA has been found in various cancer cell lines, but not in lung cancer. Therefore, this study aimed to explore the role of circFLNA in the progression of lung cancer. The target gene of circFLNA was determined by bioinformatics and luciferase reporter assay.

A novel manipulator with needle insertion forces feedback for robot-assisted lumbar puncture.

Background: The use of lumbar puncture for paediatric diagnosis and treatment, such as cerebrospinal fluid sampling and intracranial pressure measurements, is steadily increasing. However, the standard 'blind' technique makes it difficult to attain accurate needle insertion.

Methods: In this study, we developed a robot-assisted system to improve the precision of needle insertion during lumbar puncture.

Long-term exposure to ozone and sulfur dioxide increases the incidence of type 2 diabetes mellitus among aged 30 to 50 adult population.

Aims/hypothesis: Worldwide, the information regarding the associations between long-term exposure to ozone (O) and sulfur dioxide (SO) and the development of type 2 diabetes remains scarce, especially in Asia. This study aimed to investigate the long-term effects of exposure to ambient O and SO on the incidence of type 2 diabetes with consideration of other air pollutants in Taiwanese adults aged 30 to 50 years.

Methods: A total of 6,426,802 non-diabetic participants aged between 30 and 50 years old were obtained from the National Health Insurance Research Database between 2005 and 2016.

Pyrinap ligands for enantioselective syntheses of amines.

Amines are a class of compounds of essential importance in organic synthesis, pharmaceuticals and agrochemicals. Due to the importance of chirality in many practical applications of amines, enantioselective syntheses of amines are of high current interest. Here, we wish to report the development of (R,R)-N-Nap-Pyrinap and (R,S)-N-Nap-Pyrinap ligands working with CuBr to catalyze the enantioselective A-coupling of terminal alkynes, aldehydes, and amines affording optically active propargylic amines, which are platform molecules for the effective derivatization to different chiral amines.

Safety risk management for low molecular weight process-related impurities in monoclonal antibody therapeutics: Categorization, risk assessment, testing strategy, and process development with leveraging clearance potential.

Process-related impurities (PRIs) derived from manufacturing process should be minimized in final drug product. ICH Q3A provides a regulatory road map for PRIs but excludes biologic drugs like monoclonal antibodies (mAbs) that contain biological PRIs (e.g.

Covalent Bridging of Corilagin Improves Antiferroptosis Activity: Comparison with 1,3,6-Tri--galloyl-β-d-glucopyranose.

The ellagitannin corilagin and its analogue 1,3,6-tri--galloyl-β-d-glucopyranose (TGG) were found to protect bone marrow-derived mesenchymal stem cells (bmMSCs) against erastin-induced ferroptosis by cellular assays. However, the antiferroptosis bioactivity of corilagin was higher than that of TGG. Corilagin also exhibited higher antioxidant and Fe-chelation levels than TGG.

Stimuli-Responsive Micelles with Detachable Poly(2-ethyl-2-oxazoline) Shell Based on Amphiphilic Polyurethane for Improved Intracellular Delivery of Doxorubicin.

Polyurethanes (PUs) have various biomedical applications including controlled drug delivery. However, the incompletely release of drug at tumor sites limits the efficiency of these drug loaded polyurethane micelles. Here we report a novel polymer poly(2-ethyl-2-oxazoline)-SS-polyurethane-SS-poly(2-ethyl-2-oxazoline) triblock polyurethane (PEtOz-PU(PTMCSS)-PEtOz).

, a new species of (Begoniaceae) from Guangdong, China.

A new species of Begonia section Coelocentrum, W.H. Tu, B.

Mass Spectrometry-Based Discovery of New Chemical Scaffold Rearrangement Ions: Aza-biphenylene as a Novel Potent Biradical Agent in Cancer Chemotherapy.

Discovery of a new drug is time-consuming, laborious, and expensive. Herein, a novel integrative strategy for discovering potential new lead compounds has been developed, which was based on the characteristics of mass spectrometry (MS). MS was used to predict the potential forced degradation products (DPs) and metabolites of drugs by electrospray ionization and collision-induced dissociation (CID).

Acupoint stimulation and Chinese herbal medicines for the treatment of premature ovarian insufficiency: A systematic review and meta-analysis.

Background And Purpose: Acupoint stimulation and Chinese herbal medicines (CHM) are widely used in the treatment of premature ovarian insufficiency (POI), but the efficacy and safety remain controversial. This systematic review aims to evaluate the efficacy and safety of acupoint stimulation and CHM for POI.

Methods: Seven databases were searched and collected studies comparing acupoint stimulation and CHM with hormone replacement therapy (HRT) from inception to July 31, 2019.

Classification of Mild Cognitive Impairment from multi-domain features of resting-state EEG.

In recent years, electroencephalography (EEG) has emerged as a low-cost, accessible and objective tools for the early diagnosis of Alzheimer's disease (AD). AD is preceded by Mild Cognitive Impairment (MCI), typically refers to early-stage AD disease. The purpose of this study is to classify MCI patients from the multi-domain features of their electroencephalography (EEG).

Taxonomic revision of the genus (Phyllanthaceae) in Taiwan, China.

A comprehensive taxonomic revision of the genus J.R. Forst.

[Effects of 22q11 deficit syndrome on mental symptoms and cognitive function in children and adolescents with schizophrenia].

Objective: To investigate the expression of 22 q11 deletion syndrome in children and adolescents with schizophrenia and its effect on mental symptoms and cognitive function.

Methods: Case-control method was used. 134 cases of children and adolescents diagnosed with schizophrenia in hospital from April 2016 to December 2018 were selected as the case group, and the patients in the case group were divided into the two groups including 22 q11 deletion group and the non-deletion group.

Emerging functions of PRKDC in the initiation and progression of cancer.

The DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is encoded by the protein kinase, DNA-activated, catalytic polypeptide () gene. DNA-PKcs plays a major role in nonhomologous end joining DNA repair, and it has been identified to be an important factor in tumor progression and metastasis. DNA-PKcs may have opposite effects in diseases, depending on the cell and tissue types.

New Genomes From the China-Myanmar Border.

is increasingly the dominant species of malaria in the Greater Mekong Subregion (GMS), which is pursuing regional malaria elimination. lineages in the GMS are poorly characterized. Currently, reference genomes are scarce due to difficulties in culturing the parasite and lack of high-quality samples.