Enhanced biodegradation potential of Klebsiella michiganensis ES15 for acetochlor: Gene knockout, heterologous expression, molecular docking, and bioremediation.

Long-term large-scale application of acetochlor has led to its accumulation in soil, causing serious environmental pollution. In this study, Klebsiella michiganensis ES15 was isolated from the contaminated reactive sludge of an acetochlor pesticide plant and achieved 79.23 % degradation of acetochlor within 4 d after medium optimization using response surface methodology.

Discovery of Dual BCL-xL/BCL-w Degraders by Exploiting the Bis(sulfonyl)benzene Ring of ABT-263 as a Linkage Vector.

Targeting antiapoptotic proteins BCL-xL, BCL-2, and BCL-w has been extensively investigated for cancer treatment. However, robust inhibition of BCL-xL by conventional inhibitors, such as ABT-263, causes thrombocytopenia, a notable drawback that limits the clinical utility of this strategy. To overcome this on-target toxicity, BCL-xL-selective and BCL-xL/BCL-2 dual-targeting proteolysis targeting chimeras (PROTACs) have been developed as alternative therapeutic strategies.

Integrated transcriptomic and enzymatic characterization of the molecular insights into nicosulfuron biodegradation by Enterobacter ludwigii ES2.

Nicosulfuron residues pose a threat to agricultural ecosystems. Therefore, it is crucial to explore the molecular mechanisms underlying microbial degradation for effective remediation. This study elucidated the nicosulfuron degradation pathway by Enterobacter ludwigii ES2 and identified a key enzyme involved in its detoxification.

Research on gamma image reconstruction method for estimating radiation source distance using the dichotomy method.

To enhance the precision of locating unidentified radiation sources with a coded aperture camera, this study introduces a refined Maximum Likelihood Expectation Maximization (MLEM) algorithm. This algorithm incorporates the dichotomy method to estimate the distance to the radiation source, followed by a superimposition of the calculated system response matrix to predict the source's position within the imaging area. Initially, a system response matrix table is created, listing potential distances from the radiation source to the camera in ascending order.

Enhanced Biodegradation Potential of ES2 to Nicosulfuron Using Metabolomics Analysis, Functional Gene , and γ-Aminobutyric Acid.

ES2 is a microorganism responsible for the efficient degradation of nicosulfuron. The reactive oxygen species generated by nicosulfuron were scavenged by the activated antioxidant system, increase in antioxidant enzyme activity, and upregulation of related gene expression, indicating how strain ES2 responds to nicosulfuron stress. Metabolomics analysis revealed that γ-aminobutyric acid (GABA) promoted the degradation of nicosulfuron by ES2, achieving a degradation rate of 98.

Corporate litigation experience and innovation quality.

This article comprehensively examines the influence and mechanism of corporate litigation experience on the innovation quality of Chinese-listed non-financial firms in the Shanghai and Shenzhen stock markets from 2004 to 2020. The article finds that corporate litigation experience in China promotes higher innovation quality, a conclusion that remains robust after a series of robustness tests. This effect is stronger when enterprises engage in legal disputes with domestic firms, experience patent litigation, and act as plaintiffs.

Targeting Lineage-Specific Functions of NR4A1 for Cancer Immunotherapy.

Orphan nuclear receptor 4A1 (NR4A1, Nur77) plays a crucial role in regulating immune cell metabolism and function within the tumor microenvironment (TME), thus influencing cancer progression and serving as a potential therapeutic target for cancer immunotherapy. A comprehensive review discussing the multifaceted roles of NR4A1 in immune cells and the exploitation of that knowledge for therapeutic development is lacking in the field. This review explores diverse functions of NR4A1 in tumor-associated immune cells, including T cells, monocytes, natural killer cells, B cells, dendritic cells, macrophages, and neutrophils.

Hypoxia exposure impairs male fertility via inhibiting Septin2-mediated spermatogonial proliferation.

Study Question: What are the molecular mechanisms underlying hypoxia-induced male reproductive impairment?

Summary Answer: Hypoxia compromises Septin2 () transcription in spermatogonia, which impedes spermatogonial proliferation through protein phosphatase 2A (PP2A)-dependent AKT dephosphorylation.

What Is Known Already: Hypoxia is associated with impaired spermatogenesis and poor sperm parameters in men. Spermatogonia proliferation, a crucial early step in spermatogenesis, is essential for maintaining the spermatogenic cell population and ensuring sperm quality.

Elucidation of molecular mechanisms underlying degradation of nicosulfuron and its derivative by Klebsiella jilinsis 2N3 using multiomic analysis.

Nicosulfuron is a herbicide used in agricultural production. Its prolonged application causes significant ecological risks to soil and water environment. In this study, the molecular mechanisms underlying degradation of nicosulfuron and its derivative by Klebsiella jilinsis 2N3 was determined.

Spatial and Temporal Characteristics of Riverine Fungal Communities and Their Responses to Environmental Factors in China's Largest Jiang-Flavor Baijiu-Producing Region.

The Chishui River is the prime production area for Jiang-flavor Baijiu in China, with 85% of the country's production capacity. To understand the spatial and temporal characteristics of fungal communities in the water column of the Maotai reach of the Chishui River and the relationship between fungal communities and environmental factors as well as the brewing of Jiang-flavor Baijiu, we analyzed the fungal species richness and diversity, structural changes, community composition, and correlation with environmental factors in different seasons and river reaches of the Chishui River Maotai reach. It was found that species richness and diversity followed the following seasonal pattern: summer > autumn > winter ≈ spring.

Helicobacter pylori CagA elevates FTO to induce gastric cancer progression via a "hit-and-run" paradigm.

Background: Helicobacter pylori (H. pylori) infection contributes significantly to gastric cancer (GC) progression. The intrinsic mechanisms of H.

Novel microbial technologies for optimizing maize plant-soil systems: the multifunctional strain Enterobacter sp. ES1.

Background: In recent years, a new microbial technology has emerged to optimize maize field productivity and soil health. This is crucial because of the adverse effects of pesticide contamination and diseases on maize yields.

Results: Enterobacter sp.

Burden of Fungal Skin Diseases in Five East Asian Countries: An Analysis of the Global Burden of Disease Study 2021.

Introduction: The unique natural and social environments of East Asia may shape the characteristics of fungal skin diseases. However, there is a notable absence of thorough comparative analyses on this subject.

Methods: This research undertook a comprehensive analysis of the epidemiology and disease burden of fungal dermatoses across five East Asian countries (China, Japan, Republic of Korea, Democratic People's Republic of Korea and Mongolia) via the Global Burden of Disease (GBD) database from 1990 to 2021.

Proteolysis targeting chimera extracellular vesicles for therapeutic development treating triple negative breast cancer.

Proteolysis targeting chimeras (PROTACs) are an emerging targeted cancer therapy approach, but wide-spread clinical use of PROTAC is limited due to poor cell targeting and penetration, and instability in vivo. To overcome such issues and enhance the in vivo efficacy of PROTAC drugs, microfluidic droplet-based electroporation (µDES) was developed as a novel extracellular vesicle (EVs) transfection system, which enables the high-efficient PROTAC loading and effective delivery in vivo. Our previously developed YX968 PROTAC drug had shown the selectively degradation of HDAC3 and 8, which effectively suppresses the growth of breast tumor cell lines, including MDA-MB-231 triple negative breast cancer (TNBC) line, via dual degradation without provoking a global histone hyperacetylation.

Radioactive sources search method based on multi-robot and Voronoi partition.

In this paper, it is proposed to locate multiple unknown radioactive sources within a certain time limit through particle filtering and Voronoi partitioning. Firstly, with each robot as a Voronoi centroid, the entire area is partitioned. Then, the robots conduct source search concurrently through particle filtering.

Enhanced bioremediation of soils contaminated with nicosulfuron using the bacterial complex A12.

Aims: To construct an efficient bacterial complex to degrade nicosulfuron and clarify its degradative characteristics, promote the growth of maize (Zea mays), and provide a theoretical foundation for the efficient remediation of soil contaminated with nicosulfuron.

Methods And Results: Biocompatibility was determined by the filter paper sheet method by mixing Serratia marcescens A1 and Bacillus cereus A2 in a 1:1 ratio, yielding A12. The optimum culture conditions for the bacterial composite were obtained based on a three-factor, three-level analysis using response surface methodology, with 29.

Strengthening bioremediation potential: Enterobacter ludwigii ES2 for combined nicosulfuron and Cd contamination through whole genome and microbial diversity community analysis.

Nicosulfuron and Cd are common pollutants that pose significant threats to the environment and human health, particularly under combined stress. This study is the first to remediate environmental nicosulfuron and Cd under combined stress using microbiological techniques. Enterobacter ludwigii ES2 was isolated, characterized, and demonstrated to degrade 93.

GNAS knockout potentiates HDAC3 inhibition through viral mimicry-related interferon responses in lymphoma.

Despite selective HDAC3 inhibition showing promise in a subset of lymphomas with CREBBP mutations, wild-type tumors generally exhibit resistance. Here, using unbiased genome-wide CRISPR screening, we identify GNAS knockout (KO) as a sensitizer of resistant lymphoma cells to HDAC3 inhibition. Mechanistically, GNAS KO-induced sensitization is independent of the canonical G-protein activities but unexpectedly mediated by viral mimicry-related interferon (IFN) responses, characterized by TBK1 and IRF3 activation, double-stranded RNA formation, and transposable element (TE) expression.

A novel strategy for improving bowel preparation based on social software-enhanced education: A prospective, multicenter, randomized controlled study.

Background And Aim: The compliance and timeliness of oral laxatives have always been the key factors restricting bowel preparation (BP). We have constructed a novel enhanced-educational content and process based on social software (SS) for BP to optimize these issues.

Methods: A multicenter, prospective, randomized controlled study was conducted at 13 hospitals in China from December 2019 to December 2020.

Targeted Protein Degradation: Current and Emerging Approaches for E3 Ligase Deconvolution.

Targeted protein degradation (TPD), including the use of proteolysis-targeting chimeras (PROTACs) and molecular glue degraders (MGDs) to degrade proteins, is an emerging strategy to develop novel therapies for cancer and beyond. PROTACs or MGDs function by inducing the proximity between an E3 ligase and a protein of interest (POI), leading to ubiquitination and consequent proteasomal degradation of the POI. Notably, one major issue in TPD is the lack of ligandable E3 ligases, as current studies predominantly use CUL4 and CUL2.

Discovery of a Highly Potent and Selective HDAC8 Degrader: Advancing the Functional Understanding and Therapeutic Potential of HDAC8.

HDAC8 plays crucial roles in biological processes, from gene regulation to cell motility, making it a highly desirable target for therapeutic intervention. HDAC8 also has deacetylase-independent activity which cannot be blocked by a conventional inhibitor. In this study, we report the discovery of YX862, a highly potent and selective hydrazide-based HDAC8-proteolysis targeting chimera (PROTAC) degrader.

Discovery of GPR84 Fluorogenic Probes Based on a Novel Antagonist for GPR84 Bioimaging.

GPR84 is a promising therapeutic target and biomarker for a range of diseases. In this study, we reported the discovery of BINOL phosphate (BINOP) derivatives as GPR84 antagonists. By investigating the structure-activity relationship, we identified as a novel GPR84 antagonist.