Gastric metastasis subsequent to renal cell carcinoma surgery: a case report.

Gastric metastasis of renal cancer involves the dissemination of renal cancer cells to the stomach via hematogenous or lymphatic routes, leading to the formation of metastatic tumors. Renal cell carcinoma (RCC), a highly aggressive malignant tumor, exhibits a significant propensity for metastasis. Common metastatic sites include the lung, bone, liver, and brain.

Fruquintinib and sintilimab plus SOX as perioperative therapy for locally resectable advanced gastric/gastroesophageal junction adenocarcinoma: study protocol for a prospective, single-arm, phase II clinical trial.

Background: Locally advanced gastric/gastroesophageal junction (G/GEJ) adenocarcinoma faces high recurrence risks despite radical surgery. Perioperative chemotherapy (e.g.

Simple and flexible method for measuring the liquid refractive index with an electrically tunable lens.

In the parallel-plate-based method for measuring the liquid refractive index (RI), the parallel light passes sequentially through a convex lens and a parallel plate containing the tested liquid, resulting in axial displacement of the emitted light. By moving the camera, the axial displacement caused by the introduction of the tested liquid can be obtained and used to calculate the liquid RI. To avoid the mechanical adjustment of the system, a simple and flexible method for measuring the liquid RI via an electrically tunable lens (ETL) is proposed.

A Review on the Application and Mechanism of Low Temperature Plasma in the Field of Tumor Therapy.

Low temperature plasma (LTP), as an emerging cancer treatment technology, has shown significant therapeutic potential due to its unique physical and chemical properties and biological effects. This article reviews the basic characteristics of LTP and its multiple mechanisms of application in tumor treatment. LTP can induce various cell death modes, including apoptosis, pyroptosis, and autophagy, through its unique chemical and physical properties.

Simonsiella muelleri α2,6-sialyltransferase enables efficient biosynthesis of 6'-sialyllactose in both plasmid-dependent and plasmid-free engineered Escherichia coli.

6'-Sialyllactose (6'-SL) is the most abundant sialylated human milk oligosaccharide in breast milk. Its health effects have been widely confirmed, and its biological production attracts increasing attention. The metabolic pathway of 6'-SL synthesis is clear, and the typical metabolic engineering strategies for its production improvement have been reported.

HUC-MSC-derived exosomal miR-16-5p attenuates inflammation via dual suppression of M1 macrophage polarization and Th1 differentiation.

Nowadays mesenchymal stem cell-derived exosomes (MSC-Exos) have emerged as a promising cell-free therapeutic alternative to MSC-based therapies, demonstrating efficacy in treating degenerative diseases, inflammatory disorders, and autoimmune diseases. MSC-Exos transport bioactive cargoes such as proteins, lipids, mRNAs, and microRNAs (miRNAs) to the recipient cells, mediating intercellular communication to regulate immunomodulation and tissue repair. However, the exosomal miRNA profile varies dynamically based on the culture conditions and tissue sources.

PEPC-Net: Progressive Edge Perception and Completion Network for Precise Identification of Safe Resection Margins in Maxillofacial Cysts.

Maxillofacial cysts pose significant surgical risks due to their proximity to critical anatomical structures, such as blood vessels and nerves. Precise identification of the safe resection margins is essential for complete lesion removal while minimizing damage to surrounding at-risk tissues, which highly relies on accurate segmentation in CT images. However, due to the limited space and complex anatomical structures in the maxillofacial region, along with heterogeneous compositions of bone and soft tissues, accurate segmentation is extremely challenging.

Enhancement of Sialyllacto--tetraose a Biosynthesis in BL21(DE3) by Combinatorial Metabolic Engineering Strategies.

Sialyllacto--tetraose a (LST-a, Neu5Ac2,3Gal1,3GlcNAc1,3Gal1,4Glc) is a typical sialylated human milk oligosaccharide (HMO). Metabolic engineering synthesis of LTS-a should require the introduction of three key glycosyltransferases for the sequential glycosylation extension of lactose, including 1,3--acetylglucosaminyltransferase (1,3-GlcNAcT), 1,3-galactosyltransferase (1,3-GalT), and 2,3-sialyltransferase (2,3-SiaT). In this study, BL21(DE3) was engineered to produce LST-a using multilevel metabolic engineering strategies efficiently.

Correction: LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis.

[This corrects the article DOI: 10.32604/or.2024.

Serum Metabolomic Profiles Predict Sensitivity and Toxicity to Platinum-Fluorouracil Chemotherapy in a Gastric Cancer Xenograft Model.

Background: The mechanisms of chemotherapy sensitivity and toxicity are complex. Metabolomics can better reflect the status of anticancer drugs, tumors, and hosts simultaneously.

Aim: To identify metabolites and metabolic pathways linked to varying sensitivity and toxicity to chemotherapy in gastric cancer.

Efficient Biosynthesis of Sialyllacto--tetraose a by a Metabolically Engineered BL21(DE3) Strain.

Recently, the construction of metabolically engineered strains for the microbial synthesis of human milk oligosaccharides (HMOs) has attracted increasing attention. However, fewer efforts were made in the in vivo biosynthesis of complex HMOs, especially sialylated complex HMOs. In this study, we engineered BL21(DE3) to efficiently produce sialyllacto--tetraose a (LST-a) efficiently.

Highly Efficient Production of Sialyllacto--tetraose C via Screening of Beneficial β1,4-galactosyltransferase and α2,6-sialyltransferase.

Biological production of human milk oligosaccharides (HMOs) using metabolically engineered strains is a research hotspot in food biotechnology, but less effort has been made on the biological production of sialylated complex HMOs. Sialyllacto--tetraose c is the only monosialylated complex HMO in the top 15 HMOs. In this study, the metabolic pathway of LST c was constructed in BL21(DE3) by introducing three sequential glycosyltransferases: β1,3--acetylglucosaminyltransferase, β1,4-galactosyltransferase, and α2,6-sialyltransferase.

Large screen size transparent display using spectrum expanded light field holograms.

Holographic displays have the potential to reconstruct natural light field information, making them highly promising for applications in augmented reality (AR), head-up displays (HUD), and new types of transparent three-dimensional (3D) displays. However, current spatial light modulators (SLMs) are constrained by pixel size and resolution, limiting display size. Additionally, existing holographic displays have narrow viewing angles due to device diffraction limits, algorithms, and optical configurations.

Research on a Low-Cost, Low-Waste Method for Surface Flatness Improvement of WAAM Components Based on Tungsten Inert Gas Arc Remelting (TIGAR).

Wire-arc additive manufacturing (WAAM) has fully empowered the design and manufacturing of metals with its unparalleled efficiency and flexibility. However, the process has relatively poor shape control capabilities, often requiring machining post-processing. This study explores a tungsten inert gas arc remelting (TIGAR) process to improve the surface flatness of WAAM components at a low cost and significantly reduce machining waste (up to 76%), which is crucial for the sustainable development of the process.

Research Progress in Shape-Control Methods for Wire-Arc-Directed Energy Deposition.

Wire-arc-directed energy deposition (WA-DED) stands out as a highly efficient and adaptable technology for near-net-shaped metal manufacturing, with promising application prospects. However, the shape control capability of this technology is relatively underdeveloped, necessitating further refinement. This review summarizes the latest advancements in the shape control of WA-DED technology, covering four pivotal areas: the regulation of various process parameters, optimization of the deposition paths, control through auxiliary energy and mechanical fields, and synergy between additive and subtractive manufacturing approaches.

Imbalance of early-life vitamin D intake targets ROS-mediated crosstalk between mitochondrial dysfunction and differentiation potential of MSCs associated the later obesity.

Background: Obesity is characterized by excessive fat accumulation, which is related with abnormal pluripotency of mesenchymal stem cells (MSCs). Recently, there is growing evidence that the disorder of maternal vitamin D (VD) intake is a well-known risk factor for long-term adverse health outcomes to their offspring. Otherwise, less is known of its repercussion and underlying mechanisms on the different differentiation potential of MSCs.

LncRNA PCGEM1 facilitates cervical cancer progression via miR-642a-5p/KIF5B axis.

At present, the role of many long non-coding RNAs (lncRNAs) as tumor suppressors in the formation and development of cervical cancer (CC) has been studied. However, lncRNA prostate cancer gene expression marker 1 (PCGEM1), whose high expression not only aggravates ovarian cancer but also can induce tumorigenesis and endometrial cancer progression, has not been studied in CC. The objective of this study was to investigate the expression and the underlying role of PCGEM1 in CC.

Complete laparoscopic and Da Vinci robot esophagogastric anastomosis double muscle flap plasty for radical resection of proximal gastric cancer.

Objective: To investigate the application value of complete laparoscopy and Da Vinci robot esophagogastric anastomosis double muscle flap plasty in radical resection of proximal gastric cancer.

Method: A retrospective descriptive study was used. The clinicopathological data of 35 patients undergoing radical operation for proximal gastric cancer admitted to Liaoning Cancer Hospital from January 2020 to December 2023 were collected.

Highly efficient biosynthesis of 3'-sialyllactose in engineered Escherichia coli.

3'-Sialyllactose (3'-SL), one of the abundant and important sialylated human milk oligosaccharides, is an emerging food ingredient used in infant formula milk. We previously developed an efficient route for 3'-SL biosynthesis in metabolically engineered Escherichia coli BL21(DE3). Here, several promising α2,3-sialyltransferases were re-evaluated from the byproduct synthesis perspective.

Silkworm Hemolymph and Cocoon Metabolomics Reveals Valine Improves Feed Efficiency of Silkworm Artificial Diet.

Artificial silkworm diets significantly impact farm profitability. Sustainable cocoon production depends on the continuous improvement of feed efficiency to reduce costs and nutrient losses in the feed. This study used metabolomics to explore the differences in silkworm cocoons and hemolymph under two modes of rearing: an artificial diet and a mulberry-leaf diet.

Stimulated Human Umbilical Cord Mesenchymal Stem Cells Enhance the Osteogenesis and Cranial Bone Regeneration through IL-32 Mediated P38 Signaling Pathway.

Objective: Our previous study found that it could significantly increase the expression of IL32 after stimulating the human umbilical cord mesenchymal stem cells (S-HuMSCs). However, its role on the osteogenesis and cranial bone regeneration is still largely unknown. Here, we investigated the possible mechanism of this effect.

Gated CNN-Transformer Network for Automatic Cardiovascular Diagnosis using 12-lead Electrocardiogram.

12-lead electrocardiogram (ECG) is a widely used method in the diagnosis of cardiovascular disease (CVD). With the increase in the number of CVD patients, the study of accurate automatic diagnosis methods via ECG has become a research hotspot. The use of deep learning-based methods can reduce the influence of human subjectivity and improve the diagnosis accuracy.

Engineering MG1655 for Highly Efficient Biosynthesis of 2'-Fucosyllactose by GDP-Fucose Pathway.

2'-Fucosyllactose (2'-FL), the most typical human milk oligosaccharide, is used as an additive in premium infant formula. Herein, we constructed two highly effective 2'-FL synthesis producers a GDP-fucose pathway from engineered MG1655. First, and , two competitive pathway genes, were disrupted to block the invalid consumption of lactose and GDP-fucose, respectively.