Pseudouridine synthase 1-targeted therapy activates antiviral immunity to boost cancer immunotherapy.

Pseudouridine is the most abundant epitranscriptomic modification, but its cellular functions remain poorly understood. Here, we identify pseudouridine synthase 1 (PUS1) as a key driver of tumor immune evasion. Specifically, we find that PUS1 is aberrantly overexpressed in tumors and correlates with tumor malignant progression.

2-Ethylhexyl Diphenyl Phosphate Inhibited C2C12 Myoblast Differentiation by Regulating ACTC1.

2-ethylhexyl diphenyl phosphate (EHDPHP), a ubiquitous organic phosphorus flame retardant, is extensively employed in food packaging, flame retardancy applications, and other industrial processes as a crucial chemical. However, recent discoveries have underscored its diverse toxic effects, posing a severe potential threat to biological health. Skeletal muscle, vital for locomotion, relies heavily on proper differentiation during development and maintenance for strength and functionality.

Tailored Crystallization and Defect Control via Acetamide Derivatives for Efficient Tin Perovskite Solar Cells.

Tin perovskite solar cells (PSCs) have emerged as a highly promising lead-free alternative, attracting significant research interest owing to their reduced toxicity and excellent optoelectronic characteristics. However, their photovoltaic performance is severely restrained by the uncontrollable crystallization process and the resultant high defect density in the perovskite films. To address these issues, two acetamide derivatives, i.

Artificial intelligence for automatic FLS credentialing: highlighting and addressing current limitations.

Background: Artificial Intelligence (AI) can automate technical skills assessment. Applied to the Fundamentals of Laparoscopic Surgery (FLS®), AI-driven credentialing could enhance evaluation consistency while reducing costs associated with human proctoring. However, existing studies fall short of demonstrating the reliability required for high-stakes assessments, often due to limited validation and suboptimal modeling strategies.

Efficient and selective leaching of nickel and cobalt from nickel laterite ore by a combined atmospheric acid leaching-ferric chloride solution leaching process: optimization and mechanism.

Low-selectivity leaching of nickel and/or cobalt is a major problem for atmospheric acid leaching processes, while harsh leaching conditions make the pressurised acid leaching process costly. Thus, the development of an efficient and selective leaching process of cobalt and nickel under mild conditions is required. Here, a combined atmospheric acid leaching-ferric chloride solution leaching process was developed to leach cobalt and nickel selectively and efficiently over iron from nickeliferous laterite ores.

Age-specific childhood obesity and adult cholelithiasis: association and shared transcriptomic bases.

Objectives: The association between obesity and cholelithiasis has been identified. However, the causal relationship between age-specific childhood obesity and adult cholelithiasis remains unclear. In addition, the biological basis for the association between childhood obesity and adult cholelithiasis is poorly understood, which poses a challenge for preventing adult cholelithiasis in specific biological pathways.

Lipid-Modified Bilirubin Nanocrystals for the Repair of Ischemia-Reperfusion-Induced Acute Kidney Injury.

Ischemia-reperfusion (IR)-induced acute kidney injury (AKI) is a life-threatening form of AKI frequently observed in clinical settings, such as hemorrhagic shock, kidney transplantation, and cardiovascular surgery. Targeting renal antioxidant therapy may represent an effective strategy against IR-induced AKI. Bilirubin (BR), a specific superoxide anion scavenger, faces limitations in clinical application due to its poor water solubility and hepatic metabolism pathway.

A novel carbon-negative side-stream MBMBR process for municipal wastewater reclamation driven by biopolymer and nutrient recovery.

With the rapid evolution of global climate change and resource shortages, there is a growing need for technological innovation to achieve energy and carbon-neutral wastewater reclamation. In this study, a high-rate side-stream moving bed membrane bioreactor (MBMBR) process was used for municipal wastewater reclamation via COD capture for biopolymer harvesting and nutrient recovery. The results revealed excellent COD capture ability (organic conversion loading of 2.

Combining antibody-drug conjugates with immune checkpoint inhibitors: A new paradigm for breast cancer therapy.

The use of antibody-drug conjugates (ADCs) and immune checkpoint inhibitors (ICIs) has revolutionized the treatment of breast cancer. ADCs deliver cytotoxic payloads to tumor cells via antigen-targeted monoclonal antibodies, triggering direct cytotoxicity and immunomodulatory effects such as immunogenic cell death (ICD), antibody-dependent cellular cytotoxicity (ADCC), and dendritic cell activation. Preclinical and clinical studies highlight the synergistic effect of combining ADCs with ICIs: ADCs enhance tumor immunogenicity by releasing neoantigens, while ICIs reinvigorate T-cell-mediated antitumor responses by blocking the PD-1/PD-L1 or CTLA-4 pathways.

Comparison of Oliceridine and Morphine in Postoperative Analgesia in Laparoscopic Total Hysterectomy, a Randomized Double-Blind Controlled Trial.

Purpose: Compare the analgesic efficacy and safety profiles of oliceridine and morphine in patient-controlled intravenous analgesia (PCIA) after laparoscopic total hysterectomy.

Materials And Methods: This prospective, double-blind trial randomly allocated 60 patients undergoing elective laparoscopic total hysterectomy in a 1:1 ratio to receive either oliceridine (group O) or morphine (group M) via PCIA. The primary endpoint was cough numerical rating scale (NRS) scores 6 h postoperatively.

Molecular-Level Understanding of Membrane Disruption Behaviors of Antimicrobial Peptides by Gradient Boosting Algorithm-Assisted Raman Spectroscopy.

Antimicrobial peptides (AMPs) can rapidly kill bacteria via disrupting the integrity of the cytoplasmic membrane. Although the understanding of the interaction between AMPs and phospholipid membrane is essential for related drug designs, the observation of this process is still challenging, especially at the molecular level. Herein, we develop a new analytical methodology named gradient boosting algorithm-assisted Raman spectroscopy (GB-Raman) for revealing the membrane disruption mechanism of AMPs.

Strategic incorporation of metal ions in bone regenerative scaffolds: multifunctional platforms for advancing osteogenesis.

Bone serves as a critical structural framework, enabling movement and protecting internal organs. Consequently, maintaining skeletal health is a pivotal objective in bone tissue engineering. Bioactive metal ions, such as magnesium, strontium, zinc and copper, play essential roles in bone metabolism by participating in key physiological processes that sustain bone health and support regeneration.

Residual nephrogenic rests affect the long-term prognosis of Wilms tumor with bilateral nephrogenic rests.

Background/objectives: The progression of nephrogenic rests (NRs) can lead to the development of Wilms tumor (WT). The aim is to explore the factors affecting the poor prognosis associated with WT combined with bilateral NRs.

Methods: We retrospectively analyzed the clinical data of patients with WT combined bilateral NRs admitted to our center from January 2008 to December 2022.

A novel approach to sesame oil aroma generation: Synergy of enzyme-modified lipids and the Maillard reaction.

This study aimed to develop a novel method for creating roasted sesame oil (SO) flavoring under mild conditions. Fresh SO (FSO) was hydrolyzed by lipase to obtain ESO, which was further oxidized by lipoxygenase to obtain ESO. Compared with ESO or FSO, adding ESO to the Maillard reaction (MR) model comprising arginine, lysine, cysteine, serine, and glucose enhanced the consumption of glucose and amino acids, browning intensity, and formation of aroma-active alkylpyrazines, alkylfurans, thiazoles, thiophenes, MR-induced ketones and acids, while reducing lipid-derived ketones, aldehydes, and acids (p < 0.

Predictive Value of Baseline CT Radiomics for Jianpibushen Prescription Efficacy in Femoral Head Necrosis.

Objectives: To explore the predictive value of baseline CT radiomics for the 6-month and 12-month treatment efficacy of the Jianpibushen Prescription in femoral head necrosis (FHN), with the goal of optimizing treatment strategies.

Methods: Retrospectively, ARCO stage 2-4 FHN patients who underwent hip joint CT scans before receiving Jianpibushen Prescription treatment from September 2016 to December 2023 were collected. 315 patients (M/F = 210/105, median age 39.

Dual-Functional Nanoparticles to Reverse Osteoblast Senescence and Enhance Calcium Supplementation for Alleviating Senile Osteoporosis.

Senile osteoporosis (SOP) primarily arises from an imbalance between bone formation and bone resorption. The tightly regulated coupling between osteoblasts and osteoclasts limits the therapeutic efficacy of conventional anti-resorptive agents and anabolic agents. Anti-aging therapy offers a potential strategy to modify the senescent phenotype of bone-associated cells, restore cellular function, and re-establish homeostasis between bone resorption and formation.

Tranexamic acid-fatty alcohol polyoxyethylene ether conjugation/PVA foam for venous sclerotherapy vascular damage and inhibiting plasmin system.

Venous system diseases mainly include varicose veins and venous malformations of lower limbs and the genital system. Most of them are chronic diseases that cause serious clinical symptoms to patients and affect their health and quality of life. Sclerotherapy has become the first-line therapy for venous system diseases.

Mesenchymal stem cell-secreted KGF ameliorates acute lung injury via the Gab1/ERK/NF-κB signaling axis.

Background: The epithelial sodium channel (ENaC) situated in the apical membrane of alveolar epithelial type 2 (AT2) cells is beneficial to edematous fluid reabsorption in acute lung injury (ALI). Recently, mesenchymal stem cells (MSCs), particularly their secretome, has emerged as a novel approach for treating pulmonary diseases. Among these secreted factors, keratinocyte growth factor (KGF) plays a critical role in mediating alveolar epithelial repair during ALI by enhancing epithelial cell proliferation, restoring epithelial integrity, and alleviating pulmonary edema, making it a promising candidate for therapeutic strategies.

17β estradiol activates autophagy and attenuates homocysteine mediated inflammation in endothelial cells through PI3K AKT MTOR signaling.

The accumulation of plasma Hcy has been linked to vascular inflammation, which can result in vascular dysfunction. Autophagy has been the subject of extensive research in the context of hypertension-related vascular inflammation injury. As demonstrated in a preceding study, the protective effect of estradiol against vascular injury is attributable to its impact on autophagy.

Identification and verification of feature biomarkers associated with CD14 monocytes in type 1 diabetes.

Aims/introduction: Monocytes contribute to the immune dysregulation of pancreatic islets in Type 1 diabetes mellitus (T1D). This study aims to identify the feature genes of CD14 monocytes in T1D, which might offer a new perspective on immune dysregulation and potential therapeutic targets in T1D.

Materials And Methods: Two CD14 monocyte-related datasets were integrated for DEGs and WGCNA analysis.

FK506 reverses neuropathology and cognitive impairment in APPswe/PS1dE9 mice.

Background: Alzheimer's disease (AD), a chronic and progressive neurodegenerative disease, is the most common cause of dementia. An important pathological basis for AD lesions is the excessive generation and deposition of β-amyloid (Aβ) caused by increased expression of the β-secretase, known as the β-site amyloid precursor protein cleaving enzyme 1 (BACE1). Effective suppression of the BACE1 overexpression has become a key AD treatment.

Characterization of pathogenic microorganisms in diabetic foot infections and development of a risk prediction model.

This study aimed to investigate the distribution of pathogenic microorganisms in diabetic foot infections (DFIs) and develop a nomogram to predict DFIs. It included 136 diabetic foot (DF) patients hospitalized at Henan University Huaihe Hospital from November 2020 to November 2024, with 86 (63.23%) having confirmed infections.

Paxlovid for the treatment of severe or critical COVID-19 in children.

Background: Paxlovid, known for its efficacy against SARS-CoV-2, is currently limited in its use for treating pediatric COVID-19, particularly in severe or critical cases.

Methods: We conducted a study within a single-center, prospective cohort of 450 children diagnosed with COVID-19 between December 2022 and May 2023. This study included 30 pediatric patients who received Paxlovid and 60 matched controls who did not, based on factors such as age, disease severity, and underlying health conditions.

Gut microbiota-derived isovaleric acid ameliorates influenza virus infection via gut-lung axis.

H9N2 influenza virus infections represent a significant respiratory health concern, yet the functional role of gut microbiota during infection progression remains poorly understood. Here, we show that H9N2 infection causes dose-dependent alterations in gut microbial communities in a mammalian infection model, particularly the depletion of Prevotella species. Prophylactic administration of Prevotella copri improved survival and clinical outcomes in infected mice by restructuring the gut microbiome, promoting beneficial bacteria, and suppressing pathogens.