Multi-system toxicity of lead (Pb) in Procambarus clarkii: Integrated analysis of tissue damage, immune dysfunction, oxidative stress, and microbial dysbiosis via multi-omics approaches.

The element lead (Pb) exhibits significant toxicity throughout aquatic habitats. Crustaceans sit at the top of the aquatic food chain and therefore are especially susceptible to Pb-associated toxicity. Nevertheless, there is limited information on the crustaceans exposed to Pb.

Effects of beer addition on the fermentation quality and flavor development of pickled peppers.

This study explored the effects of beer as a fermentation medium additive, with beer addition levels of 25 % and 50 %, on the fermentation quality and flavor development of pickled peppers. Beer addition resulted in higher pH, reducing sugar, and nitrite levels, as well as improved textural crispness, while reducing total acidity and salinity. Microbial community analysis indicated that Firmicutes, Proteobacteria, and Ascomycota were the dominant phyla.

Metabolomics analysis of dynamic quality changes in beef during low-voltage electrostatic field combined with partial freezing storage.

The quality and shelf life of beef are significantly affected by storage conditions, emphasizing the urgent need for efficient preservation technologies to extend shelf life and reduce waste. This study evaluated the quality changes of beef during storage under low-voltage electrostatic field (LVEF) combined with partial freezing and under partial freezing alone. The results showed that during the 0-24 day storage period, LVEF effectively slowed the increases in pH, thiobarbituric acid reactive substances, total volatile basic nitrogen, and total viable counts, maintained tenderness, and delayed protein oxidation.

One-step purification using MWCNTs@chitosan composite adsorbents for effective matrix interference removal in determination of 49 Pesticides in aquatic products.

Multi-walled carbon nanotubes (MWCNTs) are known for their excellent purification properties in QuEChERS-based pesticide analysis, but their potential release and non-recyclability pose environmental and economic challenges. In this study, a novel strategy is proposed to immobilize MWCNTs with chitosan, forming the MWCNTs@chitosan composite, which enables the recyclability of MWCNTs. This composite was employed in a one-step pressure filtration purification method to simultaneously purify extracts of 49 pesticides from aquatic products.

Withdrawal Time Estimation and Dietary Risk Assessment of Sulfamethoxazole in GIFT Tilapia (GIFT ) After Oral Administration.

Sulfamethoxazole (SMZ), a widely used broad-spectrum antibiotic in aquaculture, lacks comprehensive research on its residual elimination kinetics in tilapia. This study investigated SMZ residue depletion, withdrawal periods, and dietary risks in 1-year-old GIFT tilapia (Genetically Improved Farmed Tilapia ) weighing 500 ± 50 g, following oral gavage administration of a loading dose (200 mg/kg BW on day 1) and then 100 mg/kg BW daily for 6 more days, at 22 ± 2 °C. Tissue samples (plasma, muscle, skin, liver, kidney, gill, and remaining tissues) were collected from five fish per time point at intervals from 0.

Analysis of dynamic changes and correlation between microbial community and flavor quality during fermentation.

Unlabelled: This study elucidated the succession of microbial community structures and their influence on the physicochemical parameters and volatile compounds in fermented grains (FG) of . 14 dominant microorganisms were identified. gradually replaced and , while declined at day 6 of tank fermentation (TF6).

Determination of nitrofuran metabolites in fish samples by boronate affinity extraction coupled to HPLC-MS/MS using cis diol-modified mesoporous polymer (GMA-EGDMA) microspheres as adsorbents.

In this study, we present a simple and rapid boronate affinity extraction methodology for the HPLC-MS/MS analysis of four nitrofuran metabolites (NFMs) including SEM, AOZ, AHD and AMOZ in fish samples. To enhance the efficacy of this approach, mesoporous poly(GMA-EGDMA) microspheres, abundant in cis-diols, were synthesized for boronate affinity extraction. Meanwhile, 4-fluoro-3-formylphenyl boronic acid was utilized for the derivatization of NFMs, facilitating the immobilization of boronate ligands onto their molecular structures.

Development of a semi-automated one-step purification method for determination of 63 pesticides in fish samples by HPLC-MS/MS using natural loofah sponge as a novel adsorbent.

In this study, we developed a miniaturized semi-automatic purification method based on pressure-filtration purification for sensitive HPLC-MS/MS monitoring of pesticides in aquatic products. For the first time, loofah sponge was demonstrated and utilized as a novel green, low-cost, and effective one-step purification adsorbent for fish sample. The developed method consisted of two main steps: first, target analytes were extracted from fish samples using an acetonitrile-based QuEChERS extraction method.

Determination of diazepam and its active metabolites in aquatic products and aquaculture environments using modified QuEChERS-based UPLC-MS/MS.

In recent years, the residue of psychotropic drugs such as diazepam in aquatic products has attracted widespread attention and is one of the important hidden dangers to the quality and safety of agricultural products. This study developed a modified QuEChERS method combined with UPLC-MS/MS to determine the residues of diazepam and its active metabolites, nordiazepam, oxazepam, and temazepam, in aquatic products and aquaculture environments. The important variables of the QuEChERS procedure were screened and optimized through single-factor experiments and response surface methodology.

Metatranscriptomics reveals the effect of fermentation by Pediococcus acidilactici and Rhizopus oryzae on the quality of mutton sausages and the mechanism of odor metabolism.

4-alkyl-branched chain fatty acids (vBCFAs) are key flavor-impact compounds in mutton and its derived products. Pediococcus acidilactici and Rhizopus oryzae were applied to ferment mutton sausages, and GC-MS/SIM and metatranscriptomics were employed to investigate the effects of different fermentation strategies on the quality of mutton sausages and the metabolism of 4-methyloctanoic acid (4-MOA) and 4-ethyloctanoic acid (4-EOA). The results demonstrated that inoculation with the mixed starter cultures exhibited lower pH, thiobarbituric acid, and peroxide values.

Analysis of fermentation characteristics in fermented grains across seven rounds of sauce-flavored Baijiu: Microbial communities structure, physicochemical parameters, volatile and non-volatile flavor compounds.

Pit fermentation is the critical stage for flavor formation in sauce-flavored Baijiu (SFB). This study aimed to elucidate the fermentation characteristics in fermented grains at the start (EP) and at the end (OP) of pit fermentation across seven rounds of SFB. and gradually replaced and in EP, while and maintained dominance in OP.

Isolation, structural characterization and multiple activity of a novel exopolysaccharide produced by Gelidibacter sp. PG-2.

Microbial exopolysaccharides have been extensively explored due to their distinctive structural features and physiological activities, making them suitable for diverse applications in the food and environmental applications. Current studies mainly focus on the structural and functional characterization of exopolysaccharides from known strains, with limited exploration of novel strains. In this study, a novel exopolysaccharide was produced by Gelidibacter sp.

Metabolomics-based study on the effect of low-voltage electrostatic field treatment on the storage quality of postharvest square bamboo shoots.

Low-voltage electrostatic field (LP) enhances the freezing quality of food by increasing water supercooling and improving ice crystal morphology. This study explored the effects of LP treatment on the storage quality of square bamboo shoots using physicochemical, gas chromatography-mass spectrometry, and metabolomics methods. Results showed that with prolonged storage, the LP-treated group had lower activities of peroxidase, phenylalanine ammonia-lyase, and lower levels of malondialdehyde, cellulose, and lignin compared to the control group, while superoxide dismutase and catalase activities and shear force values were higher.

Activation of microbial community and enhancement of quality by inoculating Zygosaccharomyces rouxii during the post-fermentation stage of Doubanjiang.

The prolonged post-fermentation stage of Doubanjiang imparts unique flavors but may reduce microbial vitality and increase contamination, affecting quality. This study investigated the effect of Zygosaccharomyces rouxii inoculation during post-fermentation. Results showed a 46.

TMT-based quantitative proteomics reveals the effects of electromagnetic field and freezing preservation techniques on mutton quality.

This study investigated the effects of electromagnetic field preservation (EP) and freezing storage (FS) on the quality of northern Qianbei Ma mutton. Using tandem mass tagging (TMT)-labeled quantitative proteomics and bioinformatics, it was observed that EP more effectively inhibited pH increase and maintained a* and b* values compared to FS. Furthermore, the EP group was able to better maintain the water-holding capacity and tenderness of the mutton under prolonged storage.

Investigating the metabolism of four sulfonamides in grass carp via HPLC-MS for screening candidate materials of matrix reference material.

An efficient, sensitive, and precise method for investigating the metabolism of four sulfonamides in grass carp has been established using high-performance liquid chromatography-mass spectrometry. By optimizing the experimental conditions, the method achieved a coefficient of determination above 0.999, with detection and quantification limits set at 0.

Eco-friendly chitosan microspheres as a novel one-step sorbent for the rapid purification and determination of pesticides and veterinary drug multi-residues in aquatic products with HPLC-MS/MS.

A modified QuEChERS method was developed to determine multi-class pesticide and veterinary residues in aquatic products. Chitosan microspheres were conveniently synthesized and utilized as the cleanup adsorbent in the QuEChERS procedure, showcasing rapid filtration one-step pretreatment ability for the determination of drug multi-residues in aquatic products. Compared to conventional synthetic sorbents, chitosan microspheres not only have good purification performance, but also have renewable and degradable properties.

-glycoproteomic profiling reveals structural and functional alterations in yellow primary preserved egg white under saline-alkali treatment.

The posttranslational -glycosylation of food proteins is important to their structure and function. However, the -glycoproteomics of yellow preserved egg white were rarely reported. This study explored the changes of -glycoproteome in yellow preserved eggs white after salt and alkali treatment.

Exploring the heterogeneity of community and function and correspondence of "species-enzymes" among three types of Daqu with different fermentation peak-temperature via high-throughput sequencing and metagenomics.

The enzyme activity of Daqu is an important prerequisite for defining it as a Baijiu starter. However, little is known about the functional species related to enzymes in different types of Daqu at the metagenomic level. Therefore, we analyzed the differences in enzymatic properties, microbial composition and metabolic function of three types of Daqu, namely high-, medium- and low-temperature Daqus (HTD, MTD and LTD), by combining chemical feature and multi-dimensional sequencing.

Retraction notice to "Study on the quality formation mechanism of Zao chili with enhanced fermentation by 5-1" [Food Chemistry: X 17 (2023) 100626].

[This retracts the article DOI: 10.1016/j.fochx.

Bioaccumulation and elimination, acute toxicity analysis and risk assessment of diflubenzuron in common carp (Cyprinus carpio).

Diflubenzuron has been applied in agriculture and aquaculture, and its residues in ecological environment and food chain could result in chronic exposure and long-term toxicity effects for human health. However, limited information is available regarding diflubenzuron levels in fish and associated risk assessment. This study performed the analysis for dynamic bioaccumulation and elimination distribution of diflubenzuron in carp tissues.

Study on the quality formation mechanism of Zao chili with enhanced fermentation by 5-1.

Zao Chili (ZC) is a traditional fermented pepper, which plays an important role in Chinese cooking. The aim of this study was to elucidate the effect of 5-1 on the physicochemical properties, metabolite and microbiota profiling of ZC. The physicochemical factors changed regularly with the fermentation time.

Study on the correlation between microbial communities with physicochemical properties and flavor substances in the Xiasha round of cave-brewed sauce-flavor Baijiu.

The Chishui River basin is the main production area of the sauce-flavor Baijiu. Due to the particularity of sauce-flavor Baijiu technology, a large site of workshops needs to be built for brewing and storage. Therefore, used the natural karst caves of Guizhou province to manufacture the sauce-flavor Baijiu, which has enriched the connotation of sauce-flavor Baijiu and saved valuable land resources.

Analysis of the formation mechanism of volatile and non-volatile flavor substances in corn wine fermentation based on high-throughput sequencing and metabolomics.

The purpose of this study was to reveal the relationship between core microorganisms and flavor substances in the fermentation process of corn wine. Microbial diversity, volatile and non-volatile flavor substances were detected by high-throughput sequencing (HTS), headspace solid phase micro-extraction gas chromatography-mass spectrometry (HS-SPME/GC-MS) and gas chromatography time of flight mass spectrometry (GC-TOF-MS). High performance liquid chromatography (HPLC) was used to detect organic acids in corn wine fermentation, and its physiochemical properties were tracked.

Quantitative proteomics study on the changes of egg white of yellow preserved primary chicken eggs soaked in alkali solution.

In order to investigate the changes of egg white of primary chicken eggs after being soaked in alkali solution, the tandem mass tags (TMT)-labeled quantitative proteomic technology combined with bioinformatics was conducted in this study. The results indicated that 100 differentially expressed proteins (DEPs) in yellow preserved primary egg white (YPPEW), 75 of which were highly and significantly correlated with the quality traits of YPPEW (| r | ≥ 0.9000, P < 0.